Objective:To investigate the effect of multiple-modality exercise on symptom clusters and nutritional status of nasopharyngeal carcinoma patients with radiotherapy.Methods:A total of 92 nasopharyngeal carcinoma patients who received radiotherapy in the Panzhihua Center hospital were randomly divided into research group and control group, each group contained 46 cases. The control group received routine nursing and the research group received 4-week multiple-modality exercise. The scores of M.D. Anderson symptom inventory-head & neck module (MDASI-H&N), patient-generated subjective global assessment (PG-SGA) and other biochemical nutrition index were compared before and after intervention between two groups.Results:After intervention, the scores of fatigue, poor appetite, dry mouth, mouth/throat mucus, dysgeusia were 3.77±1.30, 3.48±1.22, 3.81±1.48, 4.24±0.83, 4.94±1.63 in the research group and 4.36±1.09, 4.52±1.25, 4.39±1.24, 4.24±0.83, 6.03±2.00 in the control group, respectively. Those scores were significantly decreased in the research group compared to the control group ( t value was 1.996-3.993, P<0.05). After 4 weeks of intervention, the PG-SGA total socres, body mass index, prealbumin, hemoglobin were 2.78±0.60, (22.08±2.61) kg/m 2, (199.60±27.16) mg/L, (105.59±17.62) g/L, those indexes were 4.14±1.08, (20.63±2.63) kg/m 2, (193.42±25.57) mg/L, (102.77±18.57) g/L, the total scores of PG-SGA were significantly decreased in the research group compared to the control group ( t value was 7.356, P<0.01). Conclusions:Multiple-modality exercise can alleviate symptom burden and promote nutritional status of nasopharyngeal carcinoma patients with radiotherapy.

Biodegradable polyamines have long been studied as potential recombinant viral gene vectors. Spermine (SPE) is an endogenous tetra-amine with excellent biocompatibility yet poor gene condensation capacity. We have previously synthesized a polyspermine based on SPE and poly(ethylene glycol) (PEG) diacrylate (SPE-alt-PEG) for enhanced transfection performance, but the synthesized SPE-alt-PEG still lacked specificity towards cancer cells. In this study, folic acid (FA) was incorporated into SPE-alt-PEG to fabricate a targeted gene delivery vector (FA-SPE-PEG) via an acylation reaction. FA-SPE-PEG exhibited mild cytotoxicity in both cancer cells and normal cells. FA-SPE-PEG possessed higher transfection efficiency than PEI 25 K and Lipofectamine(®) 2000 in two tested cancer cell lines at functional weight ratios, and its superiority over untargeted SPE-alt-PEG was prominent in cells with overexpressed folate receptors (FRs). Moreover, in vivo delivery of green fluorescent protein (GFP) with FA-SPE-PEG resulted in highest fluorescent signal intensity of all investigated groups. FA-SPE-PEG showed remarkably enhanced specificity towards cancer cells both in vivo and in vitro due to the interaction between FA and FRs. Taken together, FA-SPE-PEG was demonstrated to be a prospective targeted gene delivery vector with high transfection capacity and excellent biocompatibility.