The competitive inhibition of s-GOT was made by the amber-acid(the final concen-tration was 4.6?10~(?) mol/l).m-GOT was measured by Reitman's method.The recoveryrate was 102%.The range of 10～80 KU of m-GOT was linear.The mean value of m-GOTfor 155 healthy adults was 2.8?2.7((?)+SD),76.8% of them≤5 KU.There was no diffe-rence in sex.The accuracy,normal value were similar to those in the column chromatog-raphy and immuno-assay method.The result of 66 patients revealed that m-GOT could beused as the parameter of the degree of hepatic cellular damage in virus hepatitis and inmyocardial destruction.