Objective To investigate the clinical application value of peripheral blood lymphocyte subsets expression levels in autoimmune disease(AID) among the patients with antinuclear antibody(ANA) positive .Methods 200 patients with ANA positive and 196 patients with ANA negative were selected as the experimental group and control group respectively .The experimental group adopted indirect immunofluorescence assay (IFA) and immunoblotting assay(LIA) for detecting ANA ,moreover divided into the IFA group and LIA group according to the detection results .Meanwhile the flow cytometry was adopted to detect peripheral blood T lymphocytes ,helper T lymphocytes ,cytotoxic T lymphocyte ,NK lymphocytes and B lymphocytes absolute values of each group .The detection results were statistically analyzed .Results Helper T lymphocytes ,NK lymphocytes and B lymphocytes absolute values in the experimental group were significantly lower than those in the control group ,the differences were statistically significant(P＜0 .01);in the experimental group ,helper T lymphocytes and NK lymphocytes absolute values in the IIF group were significantly lower than those in the LIA group ,the differences were statistically significant (P＜0 .01) .Conclusion Peripheral blood lymphocytes subsets can serve as the important detection indicators during the diagnosis and treatment process of AID .

Organ specific autoantibodies have important clinical value in the diagnosis , differential diagnosis, disease assessment, treatment monitoring and prognosis of organ specific autoimmune disease .In recent years , many organ specific autoantibodies have been discovered , and although there are many challenges in clinical application , its important clinical value has been gradually recognized .

Objective To explore the co-infection rate,immune function and drug resistance status among tuberculosis patients co-infected with HBV,HCV and HIV in Yunnan.Methods A total of 2271 TB patients in Yunnan province admitted to the Third People's Hospital of Kunming from April 2020 to March 2023 were collected for HBV,HCV,HIV serological tests,lymphocyte tests and drug resistance gene detection,and analysis of TB infection of HBV,HCV,HIV infection,immune function and resistance.Results Among the 2271 TB patients,499 cases were infected with TB and HBV,the infection rate was 21.97%.196 cases were infected with HCV,the infection rate was 8.63%.166 cases were infected with HIV,the infection rate was 7.31%.There were 1410 patients with simple tuberculosis,accounting for 62.09%.The infection rates of tuberculosis combined with HBV,HCV and HIV were statistically significant in different age groups(P<0.01).The absolute number expression of CD3+,CD4+ and CD8+T cells was higher in TB patients with HBV and HCV,and the differences between the two were statistically significant(P<0.05).The absolute number expression of CD3+ and CD4+T cells was lower in TB patients with HIV,and the absolute number expression of CD8+T cells was increased,and the difference between the two was statistically significant(P<0.01).The absolute number of CD3+,CD4+ and CD8+T cells was higher in female patients with tuberculosis and HIV,and the differences were statistically significant(P<0.05).In all age groups,the absolute number of CD3+ and CD8+T cells in tuberculosis patients with HIV was the lowest in 46-65 years old group,and the differences were statistically significant(P<0.05).Among the 2271 TB patients,391 were resistant to any first-line drug(single drug resistance),and the overall drug resistance rate was 17.22%.Among them,52 cases were combined with HBV drug resistance,the resistance rate was 10.42%.There were 10 cases with HCV drug resistance,the drug resistance rate was 5.10%.40 cases were combined with HIV drug resistance,the drug resistance rate was 24.10%.Drug resistance in 289 patients with simple tuberculosis was 20.50%.Among them,33 cases were single drug resistant rifampicin,accounting for 19.88%.Conclusion Tuberculosis has the highest rate of co-infection with HBV,and the immune function of TB patients co-infected with HIV is the worst.The drug resistance rate of TB patients co-infected with HIV is also the highest,with resistance to rifampicin being the most common.TB is a chronic wasting disease,with poorer immune function compared to the general population,making it more susceptible to HBV infection.TB patients who are also infected with HIV tend to have worsened immune function and increased drug resistance.

To explore the focus and trends in real-world studies in Chinese through knowledge mapping method, databases CNKI, VIP, Wanfang and Sinomed were retrieved, with 1 757 relevant articles published before September 30rd, 2020 finally included, whose bibliographical records were imported into NoteExpress to avoid duplication and check relativity. VOSviewer, a bibliometric analysis tool, was used to analyze their development. It was found that real-world studies have mainly taken shape after 2010, in which traditional Chinese medicine research plays an important role.
Bibliometrics ; China ; Databases, Factual ; Publications