Hepatocyte growth factor ( HGF),a multifunctional cytokine,plays a biological role through acting on the cell surface transmembrane receptor c-Met.The growth,invasion and metastasis of many tumors are associated with the abnormalities of HGF-c-Met signaling pathway.Studies found that HGF and its receptor c-Met have close relations with the occurrence,metastasis,invasion and prognosis of multiple myeloma.

BACKGROUND:Bone formation and development are reported to be regulated by bone morphogenetic protein2(BMP2)-induced Osterix expression. OBJECTIVE:To investigate the regulatory effect of BMP2/Osterix signaling pathway on differentiation of preosteoblasts in mice. METHODS:mRNA and protein expression of Osterix wasdetermined by real-time RT-PCR and western blot assay, respectively at various time points after mouse preosteoblasts were treated with BMP2. pcDNA3.1/myc-Osterix eukaryotic expression vector was constructed and transducted into preosteoblasts, and then mRNA expression of alkaline phosphatase, bone sialoprotein, and matrix extracelular phosphoglycoprotein wasdetected by real-time RT-PCR after transduction and BMP2 treatment. RESULTSANDCONCLUSION:Osterix mRNA expression was up-regulated when treated with BMP2 in mouse preosteoblasts, and reached the peak at 24 hours. In addition, the protein expression of Osterix was increased after BMP2 treatment. Alkaline phosphatase, bone sialoprotein, and matrix extracelular phosphoglycoprotein mRNA expression wasup-regulated after transfection of mouse preosteoblasts with pcDNA3.1/myc-Osterix eukaryotic expression vector and BMP2 treatment. Our results indicate that BMP2 regulates the synthesis of genetic markers of osteogenesis,such asalkaline phosphatase,matrix extracelular phosphoglycoproteinviaup-regulating Osterix expression in mouse preosteoblasts, suggesting BMP2/Osterix signaling pathway plays a critical role in bone development.

AIM:To evaluate the prophylactic effect of azasetron in different juncture injection on postoperative nausea and vomiting(PONV)during patients controlled intravenous ananlgesia(PCIA).METHODS:One hundred and twenty patients,scheduled for selective abdominal operations,were randomly divided into four groups:patients received an preoperative intravenous injection of 10 mg azasetron(Group A,n=30),patients received an intravenous injection of 10 mg azasetron during abdominal cavity(Group B,n=30),patients received an intravenous injection of 10 mg azasetron when the operation were finished(Group C,n=30),patients with no azasetron as control(Group D,n=30).The incidence rate and score of PONV,the visual analogue scales(VAS)and Ramsay scores were recorded at 4,8,12,24,48 h postoperatively.RESULTS:There was significant difference between the postoperative VAS during 12 to 48 h and that of 4 h postoperatively(?0.05).CONCLUSION:A preoperative intravenous injection of 10 mg azasetron can be used effectively to prevent PONV during PCIA.

AIM:To explore the changes of excitatory amino acids and N-methyl-D-aspartate receptor (NMDAR) in brain undergoing ischemia-reperfusion injury following hepatic portal occlusion (HPO) in vivo.METHODS: The electrolytes and pH of portal vein blood, the content of Glu and Asp in brain, and the expression of NMDAR mRNA were studied with blood-gas analysis, HPLC and semi-quantitative RT-PCR method in two groups(HPO or sham), respectively. Three time points(6 h,12 h and 24h after reperfusion) were included.RESULTS: HPLC measure showed that the content of cortex Glu and Asp in group HPO elevated significantly after reperfusion compared with sham group, the peaks were at 6 h and 24 h Glu:(349?145) ?g?g-1wt,(456?203) ?g?g-1wt vs (238?24) ?g?g-1wt,(225?59) ?g?g-1wt;Asp:(134?50) ?g?g-1wt,(166?50) ?g?g-1wt vs (99?24) ?g?g-1wt,(71?20) ?g?g-1wt.Moreover, semi-quantitative RT-PCR analysis discovered that the expression of NMDAR mRNA increased significantly , the subunit NR1 mRNA was higher in reperfusion 6 h and last to 12 h(1.63?0.06 vs 1.18?0.05; 1.73?0.06 vs 1.17?0.03), the peak of NR2B mRNA was in 12 h (1.75?0.04 vs 1.18?0.05) ,but they did not further increase after reperfusion for 24 h.CONCLUSION: HPO increases the content of Glu and Asp and the expression of NMDAR in brain cortex. The Glu-NMDAR pathway plays a role in the mechanism for the brain injury after HPO. The products of IR injury might be the key factor to cause EAAs acumulation in synapse space.

Extensive informed consent is a special form of informed consent.In foreign countries,the applica-tion and implementation of extensive informed consent has enhanced the circulation rate of biological samples in fu-ture research and promoted the development of the clinical research of biological samples.But it also has caused some ethical issues because of weakening informed such as:obtaining the information about the subject not in time,the research risks unknown and quiting research not in time.At present,Europe and the United States have conducted a lot of research in the implementation conditions,informed content and framework,providing a strong support for the practice of extensive informed consent.The practice of extensive informed consent has become the development direction of the biological samples research in our country.The author compared the social environ-ment and theoretical research at home and abroad and thought that our country must practice and implement it on the basis of social recognition,the improvement of ethical review mechanism and legislative protection.

With the rapid development of network technology,the data sharing and health research provides an efficient and convenient way for medical research and clinical guidance.In order to know patients' opinions on informed consent and data sharing,we investigated first-class hospitals in the coastal and inland areas with a total of 397 patients participating the questionnaire on clinical health and data sharing,reusing the remaining clinical blood samples in scientific researches,and the acceptance degree of broad consent investigation and related influencing factors.It was found that patients with strong consciousness of informed consent right tended to choose a simpler way of informed consent based on the analysis.Patients preferred social benefits than personal benefits with a strong spirit of dedication to the community.Medical institutions in China rarely applied broad consents and took relervant summative researhes,compared with the European and American countries' promotions at the national level.The research afforded the evidence,from most of patients' point of view to ethics committee in the review of such kind of studies to understand the public data sharing in privacy protection and broad consent of the individual opinion,in order to facilitate the rational development and promotion of health data sharing in China ethically.

Rapidly and accurately quantitative analysis of whole-cell sugars in some strains of actinomycetes by GC-MS was performed in this paper. After the polysaccharides were hydrolyzed, deoxidized and esterified , the alditol acetate derivates was analyzed by GC-MS. The derivates of rhamnose, ribose, arabinose , xylose, madurose, mannose, glucose, galactose were separated commendably under this condition. The ratio of the peak area was calculated to get the relative percentage of the various sugars.

OBJECTIVE:To determinate the contents of quercetin and kaempferol in Platycladus orientalis carbonisatus by RP - HPLC.METHODS:The determination was performed on Lichrospher C_(18)(250 mm?4.6 mm,5?m) with mobile phase consisted of methanol-0.2%phosphoric acid solution(55:45) at a flow rate of 1.0 mL?min~(-1).The column temperature was set at 25℃and the detection wavelength was set at 368 nm.RESULTS:The linear ranges of quercetin and kaempferol were 0.24～1.68?g(r =0.999 9) and 0.052～0.624?g(r = 0.999 8),respectively.The average recovery rates of quercetin and kaempferol were 100.91%(RSD = 0.80%,n = 6)and 99.59%(RSD = 1.07%,n = 6),respectively.CONCLUSION:The method is simple,accurate,reproducible,and applicable for the quality control of Platycladus orientalis carbonisatus.

Object To study the variation of total flavonoids in Erigeron breviscapus (Vant.) Hand.-Mazz. with the development periods, the months, the affection of the environment and genetical factors on the total flavonoids. Methods The total flavonoids in organs of E. breviscapus during the different deve-lopment periods and the months were determined. The total flavonoids of E. breviscapus in the sunny and shady habitats and the total flavonoids of E. breviscapus with different colors were compared. Results The total flavonoids of E. breviscapus mainly existed in the ground part. The total flavonoids of E. breviscapus are the highest in its flourishing period, and the lowest in its withering period. The variety of the total flavonoids in every organs of E. breviscapus in flourishing period from April to June was not significant. Illumination intensity had little affection to the total flavonoids of E. breviscapus. The colors of E. breviscapus flowers could not be the selected markers for the total flavonoids. The genetic factor played an important role in deciding the difference of the total flavonoids among the individuals. Conclusion It will be optimistic to improve the total flavonoids in E. breviscapus by genetic breeding.

Objective Hepatocellular carcinoma with bile duct tumor thrombus (BDTT) is rare,and surgical treatment is currently considered as the most effective treatment.Whether resectional surgery should be carried out on these patients remains controversial.Therefore,this Meta-analysis aimed to find out the long-term survival after resectional surgical treatment.Methods We conducted a literature search on PubMed,Embase and Web of Science from inception to September 2016.11 studies were included which involved 5295 patients.Each study was evaluated using the Newcastle-Ottawa Scale.The pooled effect was calculated and the associations between BDTT and overall survival (OS) or disease-free survival (DFS)were reevaluated using Meta-analysis with hazard ratio (HR) and 95％ confidence interval (CI).Results The HR for OS and DFS was 2.34 and 1.81,the 95％ CI were 1.26 ～ 4.36 and 1.17 ～ 2.78,respectively.Conclusion HCC patients with BDTT had a bad prognosis after hepatic resection or liver transplantation.