Objective To study the antigenicity of RNA polymerase and the relationship between the protein and the SARS-CoV replication. Methods The fragment was amplified by PCR, ligated with the prokaryotic expression vector pET32a+ and transformed into E. coli BL-21 .The expressed fusion protein identified by Western-blot and ELISA was used to detect the anti-SARS CoV IgG in different sera. Results The fusion protein was expressed successfully in E. coli BL-21. Detected by ELISA, the positive percentage of the anti-SARS IgG in the healthy donors, patients, infected animals and the rhesus administrated with the inactivated-virus were 0%, 95%, 100% and 0%, respectively. Conclusion Good antigenicity was shown in the expressed RNA polymerase. It can be used to diagnose the infection and to demonstrate the replication of SARS-CoV virus.

The blood vessels of the M.tensor fasciae latae,the iliotibial band and itsoverlying skin were dissected and measured in 30 lower limbs of 15 adult fresh ca-davers,the results were as follows:1.The branches of the lateral femoral circumflex artery may be classified intofour types:three-branch type(60.00%?8.94),dispersion type(20.00%?7.30),poly-branch type(10.00%?5.48)and two-branch type(10.00%?5.48).Amongthem,the three-branch type,which is divided into the ascending,transverse,anddescending branches,is the most common one.The caliber of the ascending branchis 2.22?0.09mm with a length of 2.70?0.22cm.The caliber of transverse branchis 2.16?0.07 mm with a length of 2.12?0.17cm.The caliber of descending branchis 2.87?0.11 mm with a length of 6.95?0.68cm.All of the three accompanyingveins consisted of vanae comitantes,the caliber and length of the vein are approxi-matly the same as those of the artery.2.The M.tensor fasciae latae is supplied by its main artery,which comes fromthe ascending branch of the lateral circumflex artery(90.00%?25.49).In a fewcases,it comes from the transverse branch(6.66%?4.55)or the deep femoralartery(3.33%?3.27).The caliber of the main artery of M.tensor fasciae latae is1.64?0.05 mm,and its length is 2.71?0.24 cm.The ascending branch and themain artery together make the vascular pedicle which length is 5.55?0.32 cm.Thecaliber and the length of the pedicle is large enough for ideal microsurgical vasanastomoses.3.The iliotibial band is supplied by the tractocutaneous artery,which comesfrom the perforating branch of myocutaneous artery of vastus lateralis and thedescending branches of lateral circumflex artery.It perforates the iliotibial bandand reaches to the subcutaneous tissues to supply the iliotibial band and its overly-ing skin.4.The skin territory of the M.tensor fasciae latae and iliotibial band issupplied by the pure cuteneous artery,the perforating branches of myocutaneousartery and the tracto-cutaneous artery.The main artery enters the muscle and sendsperforating branches into the overlying skin.All the three kinds of cutaneousarteries form abundant anastomoses in subcutaneous tissues.For this reason,if themain artery anastomoses with the artery of the host,an area in dimensions up to15?35 cm~2 of skin would survive in the anterolateral thigh.

Thirty two upper limbs of 16 adult fresh cadavers were studied.Suspension ofbarium sulfate in red latex were injected prior to medial arm flap dissection.Thearm flaps were observed and measured under the magnifying spectacles(3X),and X-ray photographs were taken.The main results obtained are summarized as follows:1.In 30 specimens,there are 386 branches arising from the brachial artery——pure cutaneous branches(67,17.36?1.93%),pure muscular and myocutaneous branches(186,48.182?2.54%)and mixed branches(133,34.46?2.42%).2.The brachial profundal artery arises from the brachial artery about 2 to 3cm.below the lower margin of pectoralis major.The caliber of the artery is 2.22?0.14mm.It supplies the triceps brachii and the skin of medial and dorsal surfaceof the upper part of arm.3.The superior ulnar collateral artery arises from the brachial artery about 6cm,below the lower margin of the pectoralis major.The caliber of the artery is1.71?0.08mm.It supplies the long and medial head of Triceps Brachii and the skinof middle and lower part of arm.It is a main artery of medial arm flap.4.The inferior ulnar collateral artery arises from the brachial artery about 18cm,below the lower margin of pectoralis major.The caliber of the artery is 1.50?0.07mm.5.The myocutaneous artery of biceps brachii arises from the brachial arteryabout 8cm,below the lower margin of pectoralis major.The caliber of the artery is1.63?0.13mm.It gives off a larger cutaneous artery.6.An abundance of anastomoses is formed by these cutaneous arteries and arich network of arteries may be seen.The caliber of some of the cutaneous arteries isabove 1mm.7.The venous drainage consisted of venae comitants.The medial arm flap is generally thin,soft and non-hair-bearing,the caliberand length of the pedicles of the arteries are large enough for microsurgical vasoanas-tomosis.It is a suitable donor site for free transfer of skin flap and the optimaldimension is 8?20cm.

As a rehabilitation strategy in the project of community development, community-based rehabilitation (CBR) can improve quality of life effectively in the community disabled, possessing the characteristics of wide coverage, convenience, being economical and effectiveness, so it has been developed and promoted in our country. At present, there are various problems in CBR, which could not meet the diverse demand of people with disablities in community. As a result, it is important to propagandize rehabilitation concept, built rehabilitation team, strengthen multidisciplinary cooperation and emphasize the development of the disabled rehabilitation system in rural community.

Objective To introduce the procedures and to evaluate the application value of the optical navigating interventional MR-guided percutaneous biopsies and therapies. Methods Thirty-five of 54 patients (33 males, 21 females) underwent percutaneous biopsies and 19 of 54 patients underwent percutaneous therapies under optical navigating interventional MR-guidance. The age ranged from 9 months to 78 years (mean 38.8 years). All patients were performed under a 0.23 T open MRI system (Proview, Philips Medical System) with optical navigating system (iPath 200, Philips Medical System). The needle and bone biopsy drill were MR-compatible (Daum, Germany). Results Punctures were accurate in all patients (54/54). 33 of 35 biopsies got the pathological diagnosis. All therapies (19/19) got the definitive curative effect. All patients had no obvious complications, such as severe hemorrhage, viscera or nerve injury.Conclusion iPath 200 interventional MR-guided percutaneous biopsies and therapies are accurate and safe techniques.

OBJECTIVE: To evaluate the usefulness of monitoring transcranial electrically stimulated motor evoked potential (MEP) and its impact on postoperative motor function after surgical clipping of intracranial aneurysms. METHODS: A total of 69 aneurysm patients were monitored for MEP during surgery. The postoperative and preoperative neurological function variation and the correlation between them were compared. RESULTS: MEP deteriorated in 9/68 patients, 6 of the deteriorated MEP returned to normal within 1-40 min, and no new motor deficit emerged. 3 of MEP failed to return to the baseline, which were consistent with postoperative motor function deficit. CONCLUSION Changes in MEP could serve as early indication of the cerebral ischemia, predicting postoperative motor function and providing a guide to a safe time for temporary clipping. FNMEP monitoring is a safe and reliable tool for the integrity of facial nerve pathway in giant aneurysm surgery.
Adolescent ; Adult ; Evoked Potentials, Motor ; physiology ; Female ; Forecasting ; Humans ; Intracranial Aneurysm ; physiopathology ; surgery ; Male ; Middle Aged ; Motor Neurons ; physiology ; Neurosurgical Procedures ; instrumentation ; methods ; Postoperative Period ; Surgical Instruments ; Young Adult