Objective To investigate the therapeutic mechanisms of Qingyi decoction on acute pan-creatitis in rats.Methods Forty-eight healthy male SD rats,weighing 250 ~300g,were randomly divided into 4 groups(n =12 each):sham operation group(group A),model group(group B),Qingyi decoction group(group C),and Sandostatin group(group D).AP models were established by injecting 5% sodium taurocholate(1ml/kg)into the biliopancreatic duct in group B,C,D.Qingyi decoction was administered oral-ly 1ml/100g)after model in group C and repeated after 12h.Sandostain (20μg/kg)was administrated by subcutaneous injection after model in group D and repeated after 12h.Groups A and B received equal vol-ume of normal saline.All rats were sacrificed 24 hours after AP modeling,At the same point time,the lev-els of serum amylase,tumor necrosis factor-α(TNF-α),interleukin-8 (IL-8)of the rats were measured, and the histopathological changes in pancreatic tissues were observed.Results The levels of serum amyl-ase,TNF-αand IL-8 were remarkably lower in Qingyi decoction group comparing with other group (P <0.05),When compared with the model group,the pathological manifestations in the pancreatic tissues were significantly attenuated in the Qingyi decoction group (P <0.05).Conclusions It indicated that Qingyi decoction can inhibit the release of TNF-αand IL-8 to reduce inflammation of SD rats with AP.

Objective To analyze the distribution and antibiotic resistance of the clinical bacterial isolated from the intensive care unit (ICU) of Tongren Hospital during 2013-2017.Methods All the bacterial isolates were identified by Vitek-2 Compact system.Antimicrobial susceptibility testing was carried out using Kirby-Bauer method or automated systems.The susceptibility data were analyzed using WHONET 5.6 software.Results were interpreted according to CLSI M100-S27th breakpoints.Results A total of 1 049 clinical isolates were collected,including 331 (31.6％) strains of gram positive bacteria and 718 (68.4％) strains of gram negative bacteria.The overall prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Staphylococcus aureus and methicillin resistant coagulase negative staphylococci (MRC-NS) in coagulase-negative Staphylococcus were 84.5％ and 80.2％.No Staphylococcus strains were found resistant to vancomycin or linezolid.The resistance rates of E.faecalis strains to most drugs tested were lower than those of E.faecium.Polymerase chain reaction (PCR) amplification and sequencing analysis indicated that the only one vancomycin-resistant strains isolated from E.faecium was vanM type.The resistance rates to carbapenems of Klebsiella pneumoniae,Pseudomonas aeruginosa,and Acinetobacter baumannii respectively were 50.7％,50.4％,and 82.7％.Conclusions Antibiotic resistance is still very serious in ICU.It is necessary to maintain good practice in surveillance of bacterial resistance,and strengthen management of clinical use of antimicrobial agents and hospital infection control.