1. The impact of meisoindigo on apoptosis and proliferation of SET2 cell line by JAK-STAT pathway
Chenglan LYU ; Jinqin LIU ; Meng CHEN ; Bin CHEN ; Zhijian XIAO
Chinese Journal of Hematology 2019;40(1):29-34
Objective:
To observe the effect of meisoindigo on apoptosis and proliferation of JAK2/V617F heterozygous mutation cell line-SET2 cell line to further explore the role of JAK-STAT pathway in this effect.
Methods:
Cell apoptosis after treated with different concentration of meisoindigo (0, 5, and 10 μmol/L) was evaluated by flow cytometry at different time points (24, 48, 72 h). Cell proliferation with CCK8 test was evaluated at different time points (24, 48, 72, 96 h) after administered with different concentration of meisoindigo (0, 5, 10, and 20 μmol/L). After treatment with different concentration of meisoindigo (0, 5, 10, and 20 μmol/L), SET2 cells were collected after 12 h, and then cultured in incomplete methylcellulose-based medium for clone formation. JAK-STAT signaling pathway and apoptosis related protein by Western blot test were evaluated 12 h after administered with different concentration of meisoindigo (0, 5, 10, and 20 μmol/L).
Results:
At different time points after treated with meisoindigo, the apoptosis rate of SET2 cell lines increased (