1.Orthogonal Experimental Study on Dryness of Weishu Powder by Microwave Technique
Xiaobin XU ; Xiangping LI ; Yiying DU ; Chenghao ZHENG
China Pharmacy 1991;0(06):-
OBJECTIVE: To select the optimal microwave technique for drying Weishu powder. METHODS: An orthogonal experiment was carried out to investigate the content of water in Weishu powder by taking drying time, microwave power, and granularity of the powder as indices. RESULTS:Weishu powder crushed and filtered through the sieve with 24 meshes then dried discontinuously for 6min(1.5min?4)with low-power microwave(800W?58%)achieved the best results. CONCLUSION: The optimal technique is effective and time saving .
2.Clinical Observation of Needling QIN's Eight Head Acupoints for Insomnia
Yufang HONG ; Chenghao DU ; Hong XU ; Weimin NI ; Liangfu QIN
Shanghai Journal of Acupuncture and Moxibustion 2017;36(6):715-718
Objective To observe the therapeutic efficacy of needling QIN's eight head acupoints in treating insomnia.Method Sixty patients were randomized into QIN's eight head acupoints group (30 cases) and a conventional acupuncture group (30 cases). The modified Spiegel sleep questionnaire was evaluated respectively after 4-week and 8-week treatment.Result After 4-week treatment, there was a significant difference in comparing the modified Spiegel sleep questionnaire between the two groups (P<0.05), and QIN's eight head acupoints group was superior to the conventional acupuncture group; after 8-week treatment, there was no significant difference between the two groups in comparing the modified Spiegel sleep questionnaire (P>0.05).Conclusion QIN's eight head acupoints group had a more significant therapeutic efficacy compared to conventional acupuncture group after 4-week treatment.
3.Treatment of Toothache by Puncturing Hegu (LI 4)
Bing WANG ; Cuiying ZHANG ; Junqi ZHANG ; Yihuan SU ; Chenghao NI ; Wenbo LI ; Xin XU ; Yao XU
Journal of Acupuncture and Tuina Science 2007;5(5):314-316
Objective: To observe the clinical effect in the treatment of toothache by puncturing Hegu(LI 4). Methods: Two hundred and seventy-eight cases of the patients were randomly divided into the treatment group (139 cases) and the control group (139 cases). The treatment group was treated by puncturing Hegu (LI 4) with electric stimulation and the control group, by puncturing Chenshan (BL 57) with electric stimulation, for 3 days as one course of the treatment,to grade pain and observe the anesthetic effect respectively. Results: There was no significant difference in the remarkable effective rate between the two groups before withdrawal of the needle in the treatment for one day (P>0.05). In the other times, the remarkable effective rate was obviously higher in the treatment group than in the control group (P<0.05). Conclusion:Acupuncture on Hegu (LI 4) is effective in treating toothache.
5.Optimization of parameters of passive cutaneous anaphylaxis in rats
Wenpei CHEN ; Ziyang ZHANG ; Chenghao ZHOU ; Han LIU ; Tong XU ; Yulin GUO ; Baoqin LIN
Chinese Journal of Pharmacology and Toxicology 2016;30(7):747-753
OBJECTIVE To optimize the parameters of passive cutaneous anaphylaxis(PCA)in rats immunized by ovalbumin(OVA). METHODS 1-2 month-old Sprague-Dawley rats were immu?nized by ip injection of OVA(0.2,1.0 and 5.0 mg per rat)mixed with complete Freund′s adjuvant once every other day 3 times. Serum was collected on the 12th-16th days after final immunization. Then the rats were intracutaneously injected with sensitized serum and then stimulated by iv injection of the same dose of OVA mixed with Evans blue after a latent period of 0.5,1.5,3,6,12,24,36,48 and 60 h. Finally,the diameters of blue spots in the skin were measured at stimulation. RESULTS Serum total-IgE(T-IgE)and OVA-specific IgE(sIgE)levels increased significantly and reached the peak on the 3rd-7th days and 12th-16th days after final immunization,respectively. There was no correlation between the serum T-IgE level and OVA-sIgE level when the rats were immunized with OVA at OVA 0.2-5.0 mg per rat. The rats experienced PCA after injection of OVA 1.0 and 5.0 mg per rat. Diameters of blue spots in the skin reached the maximum value after rats were sensitized for 0.5-3 h. Moreover,the shape,color and size of blue spots were better 30-60 min after stimulation. CONCLUSION Optimized PCA is as follows:1-2 month-old rats are immunized on the 1st,3rd and 5th days by ip injection of OVA 1.0-5.0 mg. The immunizing serum is collected at 12-16 d after final immunization. The rats are stimulated by OVA and Evans blue after a latent period of 0.5-3 h. Diameters of blue spots in rats′ skin are then measured 30-60 min after stimulation.
6.Therapeutic Observation of Electroacupuncture at Cervical Jiaji (EX-B2) Points plus Behavioral Intervention for Cervical ;Spondylosis
Deli SUN ; Lizhong XU ; Chenghao NI ; Dalong CHEN ; Pengyuan CAO ; Haifeng MA ; Hong XU ; Jian PEI ; Yi YAO
Shanghai Journal of Acupuncture and Moxibustion 2016;35(12):1451-1454
Objective To observe the clinical efficacy of electroacupuncture at cervical Jiaji (EX-B2) points plus behavioral intervention in treating cervical spondylosis. Method The cervical spondylosis patients were randomized into two groups at a ratio of 3:1, 90 cases in the electroacupuncture group and 30 cases in the medication group. The patients who received electroacupuncture were also given cupping and behavioral intervention (raising head for 1 min every 20-30 min and correcting sleep habits). The clinical efficacy was evaluated according to the symptoms and body signs assessment scale. Result Respectively after 4-week, 8-week, 4-month and 6-month treatments, the clinical control rate, markedly control rate and total effective rate in the electroacupuncture group were significantly higher than that in the medication group. Conclusion Electroacupuncture at Jiaji points plus behavioral intervention is an effective solution to prevent and treat cervical spondylosis.
7.Pathogenic features and risk factors of hospital-acquired pneumonia in patients with acute spontaneous intracerebral hemorrhage in intensive care unit
Xuan LU ; Meng XU ; Wenqing MENG ; Zhenghui PAN ; Chenghao LI ; Wenjing ZHAO
Chinese Journal of Clinical Infectious Diseases 2017;10(5):347-352
Objective To analyze the pathogenic features and risk factors of hospital-acquired pneumonia in patients with acute spontaneous intracerebral hemorrhage (sICH) in intensive care unit (ICU).Methods The clinical data of 110 patients with sICH admitted in ICU during January 2015 and February 2017 were collected.Patients were divided into hospital-acquired pneumonia group (HAP group,n =66) and non-HAP group (n =44).Multivariate Logistic regression was used to study the risk factors of HAP,and pathogen distribution and drug susceptibility were analyzed.Results Multivariate Logistic regression demonstrated that long-term mechanical ventilation (OR =1.028,95% CI 1.012-1.044,P < 0.01),lower score of glasgow coma scale (GCS) (OR =1.550,95% CI 1.148-2.093,P < 0.01),prolonged hospital stay (OR =1.131,95% CI 1.046-1.224,P <0.01) and underlying diseases more than two forms (OR =9.793,95% CI 1.012-1.044,P < 0.01) were the independent risk factors of HAP,while high plasma albumin level was protective factor for HAP (OR =0.897,95% CI O.811-0.992,P < 0.05).One hundred and eighty-three bacterial strains were isolated from 66 patients,the top 4 pathogens were Acinetobacter baumannii (28.96%,53/183),Klebsiella pneumonia (15.85%,29/183),Pseudomonas aeruginosa (13.11%,24/183) and Staphylococcus aureus (12.02%,22/183).Acinetobacter baumannii,Klebsiella pneumoniae and Pseudomonas aeruginosa were highly resistant to the majority of antibiotics,some of which even reached 100%.Staphylococcus aureus showed high resistance to macrolides,fluoroquinolones and β-lactam antibiotics.Conclusions There is high incidence of HAP in patients with sICH,and the pathogenic bacteria are mainly gram-negative bacteria.Effective prevention and treatment measures should be taken to reduce the incidence of HAP for patients with sICH in ICU.
8.Correlation of length of vascular segment in vitro with endothelin-1 metabolism of endothelial cells--study on accumulative effect of tensile stress in the upper endothelial cell membrane. III.
Guixue WANG ; Chenghao XU ; Shaoxi CAI ; Qiang FENG ; Yuanliang WANG
Journal of Biomedical Engineering 2002;19(4):606-610
A few of flow experiments were utilized to verify a theoretical hypothesis proposed by Fung and coworker which showed that the tensile stress in the upper cell membrane of the vascular endothelium could accumulate upstream against the direction of blood flow. Endothelial cells from replicate human umbilical vein segment (HUVSEC) in vitro with length of 11 cm and 21 cm were exposed to the same pulsatile laminar shear stress averaged of 0.12 N/m2 for 42 hours. The average production rate of endothelin-1(ET-1), at 11 cm segment is 50% lower than that at 21 cm segment(16.93 +/- 0.89) vs. (26.13 +/- 1.79) pg/cm2.h respectively. The average production rate of ET-1 under pulsatile laminar flow was significantly higher than that under steady laminar flow. It showed that, high correlation of the length of HUVSEC with their ET-1 metabolism exists, suggesting that the tensile stress in the upper endothelial cell membrane could accumulate.
Cell Membrane
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physiology
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Endothelin-1
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metabolism
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Endothelium, Vascular
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cytology
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physiology
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Humans
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In Vitro Techniques
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Infant, Newborn
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Stress, Mechanical
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Umbilical Veins
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anatomy & histology
9.Analysis of missed diagnosis and risk factors in patients with negative initial prostate biopsy with PI-RADS score>3
Liangyong ZHU ; Xuefei DING ; Yang LUAN ; Tianbao HUANG ; Shengming LU ; Chenghao GUO ; Yaozong XU ; Fei WANG
Chinese Journal of Urology 2020;41(9):667-671
Objective:To analyze the risk of missed diagnosis in patients with PI-RADS score>3 and negative prostate initial biopsy and to explore its risk factors.Methods:The clinical data of 268 patients with negative prostate biopsy in Northern Jiangsu People's Hospital from May 2013 to December 2018 were retrospectively analyzed. The patients were divided into observation group (PI-RADS score>3) and control group (PI-RADS score≤ 3) according to different PI-RADS scores. There were insignificant differences in age [(67.4(60.0, 74.0)years and 65.6(66.5, 72.0)years], prostate volume of initial biopsy [62.4(40.0, 72.0)ml and 60.8(38.0, 77.0)ml], biopsy cores [ 20.6(18.0, 22.0)cores and 20.4(18.0, 22.0)cores] between the observation group (n=124) and the control group(n=144)(all P>0.05). But there were significant differences in PSA [17.5(6.5, 23.0)ng/ml and 11.5(6.3, 12.0)ng/ml], PSAD[0.316(0.128, 0.363)ng/ml 2 and 0.211(0.106, 0.256)ng/ml 2], prostate inflammation of the initial biopsy [70 (56.5%) and 32 (22.2%)] between the observation group and the control group(all P<0.05). According to the follow-up results after the initial biopsy, the two groups of repeated biopsy were compared.Furthermore, Logistic regression was used to conduct univariate and multivariate analysis to explore the risk factors of patients with PI-RADS>3 for positive repeated biopsy. At the same time, the receiver operating characteristic curve (ROC curve) was used to analyze the accuracy of the risk factors. Results:There were significant differences in repeated biopsy rate [ 27.4%(34/124)and 14.6%(21/144)], CsPCa detection rate[ 41.4%(14/34) and 4.8%(1/21)]between the observation group and the control group(all P<0.05). The positive rate of repeated biopsy in the observation group (41.1%) was higher than that in the control group (23.8%), but there was no statistical difference ( P=0.248). The risk of positive repeated biopsies in the observation group was 2.24 times than that in the control group. Univariate analysis found repeated biopsy PSA ( P =0.02, OR=1.438, 95% CI 1.161-1.896), PSA ratio (repeated biopsy PSA/initial biopsy PSA) ( P=0.011, OR=10.087, 95% CI 1.714-59.36) were risk factors for positive of repeated biopsy in patients with PI-RADS score >3. Multivariate analysis also found that repeated biopsy PSA ( P=0.017, OR=1.15, 95% CI 1.076-2.123), PSA ratio ( P=0.032, OR=10.2, 95% CI 0.883-116.168) were risk factors for positive repeated biopsy. ROC curve analysis, the accuracy of repeated biopsy PSA (AUC=0.971, P<0.001, 95% CI 0.926-1.000), PSA ratio (AUC=0.839, P=0.001, 95% CI0.707-0.971) to predict positive of repeated biopsy were high. The cut-off values were 21.3 ng/ml and 1.4, respectively. The accuracy was higher when combines repeated biopsy PSA with PSA ratio (AUC=0.993, P<0.001, 95% CI 0.974-1.000). Conclusions:Patients with negative PI-RADS score > 3 have a higher risk of missed diagnosis of CsPCa than those with PI-RADS score≤3. When PSA>21.3 ng/ml and PSA ratio>1.4 during follow-up, the possibility of missed diagnosis in the initial biopsy is high.
10. Application of modified transperineal template-guided prostate biopsy in the diagnosis of prostate cancer
Xuefei DING ; Yang LUAN ; Fei WANG ; Yaozong XU ; Tianbao HUANG ; Chenghao GUO ; Liangyong ZHU ; Guangchen ZHOU ; Xiao GU
Chinese Journal of Urology 2019;40(10):763-767
Objective:
To investigate the clinical value of modified transperineal template-guided prostate biopsy (mTTPB) in the detection of prostate cancer.
Methods:
A total of 217 patients were enrolled in this study. All the patients were randomly divided into 2 groups. The control group (