Objective To study the effects of iodine on the proliferation activity of fibroblasts at different doses. Methods The cultured fibroblasts were treated with different dose iodine(100 ?g/L, 500 ?g/L, 1 000 ?g/L, 3 000 ?g/L, 5 000 ?g/L, 7 000 ?g/L, 9 000 ?g/L, 11 000 ?g/L)for 24 h and observe the morphology of fibroblast. MTT colorimetry was used to detect the fibroblast proliferation activity. Results Iodine could significantly increase the fibroblast proliferation activity at certain range of concentration (7 000 ?g/L). The proliferation activity was highest from 5 000 ?g/L to 7 000 ?g/L, the differences were significant compared with the iodine free control. Conclusion Iodine, the exposure level is 7 000 ?g/L.

Objective To investigate the character and risk factors of coronary artery disease in prediabetes. Methods The 193 patients with coronary artery disease were divided into 3 groups according to the American diabetes associations (ADA) standard of diabetic diagnoses. (1) Normal glucose tolerance group(NGT group,122 cases). (2) Impaired glucose tolerance group (IGT group,39 cases). (3) Diabetes mellitus group (DM group,32 cases). All patients were checked by the coronary angiographic semiquantitative (CAG) and the 75g oral glucose tolerance test. Blood glucose, insulin, TG, TC, HDL C, blood pressure, BUN and Cr were determined. The coronary artery changes were analysed by CAG, and the severity of coronary artery stenosis were evaluated by AHA standards. The risk factors in coronary artery disease were analysed by pluralized straight line statistics. Results The coronary multivessel changes and the severity of coronary artery stenosis and calcification were more frequent in IGT group than in NGT group (66 5% vs 35 1%,8 9?3 6 vs 6 5?3 9,6 6?6 1 vs 3 9?4 1,P

AIM: To investigate differentiation of CD34~+ cells in human umbilical blood into eosinophils under the condition of cell culture in vitro.METHODS: CD34~+ cells were separated and purified from human umbilical blood.The cells were divided into negative group,IL-5 group and allergic rhinitis serum group.The differentiation ability of the cells was measured by flow cytometry,HE staining and electron microscope at the first day,second day, 7th day,14th day and 28th day culture.RESULTS: The proportion of CD34~+ cells in IL-5 group and allergic rhinitis serum group were decreased at the second day.The proportion in allergic rhinitis serum group was lower than that in IL-5 group significantly.The typical structure of eosinophils was observed at the second day.CONCLUSION: The allergic patient serum and IL-5 induce differentiation of CD34~+ cells in human umbilical blood to eosinophils.

Objective Minimally invasive technique was used in the treatment of pectus carinatum. Indication, techni-cal details and perioperative complication are discussed in this paper. Methods From March 2008 to January 2009, 6 patients with pectus carinatum underwent minimal invasive operation by using the principle of Nuss procedure in which pressure applied through a curved steel bar that was placed subcutaneously anterior to the sternum, via lateral thoracic incisions. Preoperative CT scan were performed and Hailer index was used as operative indication. Results The average age of patients was (13.6 ± 1.94) years (range, 10 - 16 years). They were all males. The mean operation time was (104.17 ± 15.63) minutes. The av-erage blood loss was (5.17±2.56) ml. The mean hospital stay was (5.67±0.82) days. Postoperative follow-up was 1 -11 months. Displacement of the stablizer was the only complication that was corrected by further fixation procedure. COnClusion The minimally invasive technique for the treatment of peetus earinatum is safe and effective with minimal complications. It can be selected as an alternative techniques and the appropriate age for this procedure is 10-16 years.

Objective To study the anatomical location and pathology of membranous obstruction of the inferior vena cava(IVC)in Budd-Chiari syndrome(BCS)with research on the etiologic mechanism and pathology.Methods Analysis of 100 normal adults was performed including the gross anatomy of IVC segment from the level of diaphragm up to right atrium.The conventional,microscopic pathologic examination of the biopsy sampling IVC obstruetice mambrane tissue in 70 cases toghther with the complete resected membrane from the radical therapy for 20 cases of BCS,were collected and under investigation.Results The macroscopic examination revealed the obstructive membrane in one case(1%)localizing at the diaphragmatic level,approximately 28 mm,away from the IVC entrance into the right atrium and a newly found valvula was seen on the left lateral wall of the upper part of the hepatic vein orifice.Simultaneously,47% adults showed Eustachiun valve existing in IVC near the entrance to right atrium.Microscopy confirmed all the forementioned membranes consisting of vascular valvular structures.Among them(21/70),30% showed additional organized thrombus formations,and 9%(6/70)with a few amount of inflammatory cellular infiltrations.The total intact resection membrane was continuous with the vascular wall under microscopic examination.Conclusions The first newly report of the existence of a special valvula at the diaphragmatic level of IVC reveals the possibility of high correlation with the occurance of IVC membranous obstruction type in BCS.

Objective: To investigate the effects of different concentrations of vitamin E (VE) on angiogenesis and its possible mechanism. Methods: The rings of rat aorta were embedded in gels of collagen and cultured in a serum free medium for 28 days. Curves of microvessels growth were generated by counting the number of newly formed microvessels every day with an inverted microscope. Photos were taken at the same time. Expression of factor FVIII related antigen (FVIII RAg) in newly formed microvessels was detected by immunohistochemistry. Concentration change of FVIII R in medium was evaluated by ELISA. Results: Both 0.2 g/L and 0.1 g/L VE showed significant inhibitory effect on angiogenesis (P0.05). The inhibitory effect of VE was expressed in the phases of microvessel growth and decline (10-25 d). Conclusion: 1.Three dimensional collagen gels culture of microvessel in serum free medium can be used as a sensitive assay for study of soluble and solid phase angiogenic agonists and antagonists. 2. Both 0.1 g/L and 0.2 g/L VE inhibit angiogenesis, and VE has no toxicity and no drug resistance.

The corrosion rates of TiNi, CoCrNiW and CoCrNiMo were measured in Tyrode's solution with potentiodynamic linear polarization, fore-point weak polarization, Cao Chunan weak polarization, transient linear polarization and atomic absorption spectroscopy. Results indicated that corrosion rates of these three alloys were very low due to their excellent corrosion resistance and the corrosion resistance of CoCrNiMo was the best. Corrosion rates of TiNi, CoCrNiW and CoCrNiMo were 0.691, 0.0595, 0.0490 micron/a and 0.0528, 0.0383, 0.0387 micron/a, respectively. The results measured by the first three methods were about ten times of those by the latter two methods, this was related to the applicability of each method and the alloy surface state. Transient linear polarization technique can determine low corrosion rate conveniently and quickly. Atomic absorption spectroscopy method, determining directly the concentration of ion in solution, and thus provide reference for material biocompatibility. And these two methods are properly used in measuring corrosion rates for biomedical materials.
Biocompatible Materials ; chemistry ; Cobalt ; chemistry ; Corrosion ; Materials Testing ; methods ; Nickel ; chemistry ; Spectrophotometry, Atomic ; Titanium ; chemistry

Electrochemical constant current linear polarization and atomic absorption spectroscopy were used to measure the corrosion rate of coronary stents made of 316L and 317L stainless steel in 30 degrees C Tyrode's solution. The results indicated that the corrosion rate of 316L and 317L stainless steel was 21 X 10(-3) microm/a, 9.8 X 10(-3) microm/a and 0.8 X 10(-3) m/a, 0.6 X 10(-3) microm/a, respectively. All corrosion rates were lower than the medical materials corrosion rate criteria, i.e. 0.25 microm/a. Moreover the corrosion resistance of 317L stainless steel was much higher than that of 316L stainless steel. The results from atomic absorption spectroscopy may correctly reflect the quantity of releasing metal ions in the solution.
Biocompatible Materials ; chemistry ; Corrosion ; Materials Testing ; Spectrophotometry, Atomic ; Stainless Steel ; chemistry ; Stents ; Surface Properties

Adolescent ; Adult ; Antigens, CD34 ; blood ; Case-Control Studies ; Eosinophils ; Female ; Humans ; Interleukin-5 ; blood ; Leukocyte Count ; Male ; Middle Aged ; Rhinitis, Allergic, Perennial ; blood ; Young Adult