Point of care testing(POCT)is undergoing tremendous change in recent years driven by biotechnology and laboratory medicine.Attributed to its high performance,rapidity,convenience and instantaneity,POCT has been widely used in more and more fields.In this article,the categories,principles and applications of POCT in current military operations,disasters and emergency responses are elaborated.Furthermore,the future dcvelopment trends of military and emergency responses POCT are discussed.

Objective To design a micro-traumatic way that can correct the elbow valgus or varus deformity in children. Methods Thirty-eight cases were treated with the self-made Kirschner with saw, the diameter of 1.5 mm. To observe and analyse the clinical effect in the healing of the bone, the improvement of the carrying angle, the function of the elbow and the complications. Results All the patients were followed up 6 months to 3 years.The areas of the cut bone healed without injuries of nerve, vascular and epiphysis. The carrying angle recovered from 8° to 15° (mean 13.5°). During the follow-up, there were no lost of the carrying angle and no herniation of external condyle of humerus and no statistical difference in appearance.According to Cassebaum marking standard, there were 29 cases excellent,8 cases good, 1 case fair, with good rate of 97.37%(37/38). Conclusions The new technique is micro-traumatic,safe and easy to operate.It is an ideal treating method and worthy of clinical application.

Salivary gland adenoid cystic carcinoma is one of the most common malignant salivary gland tumors.Its invasion and metastasis are multi-stage and multifactorial processes,in which complex mechanisms and multiple signaling pathways are involved.Here,this article reviews the research progress in signaling pathways of salivary gland adenoid cystic carcinoma.

Objective To retrospectively analyze the expression of SARS-CoV (IgG) in the serum of hospitalized children who were not suffering from SARS, and retrospectively evaluate the difference of generation of the antibody between the children and adults. Methods Serum samples were collected from 85 hospitalized children, 500 health-check adults, 976 hospitalized adults, and 156 adults recovered from severe acute respiratory syndrome (SARS), and SARS-CoV (IgG) in the specimens was determined by ELISA and biologic chip. Results The level of serum SARS-CoV (IgG) in childhood patients was significantly higher than that in adult patients (17.6% vs 6.3%, P

Objectives To investigate antimicrobial resistance and beta-lactamase production of Moraxella catarrhalis isolates from respiratory tract in children and to understand the characteristics of BRO beta-lactamase gene. Methods From June 2011 to Sep-tember 2012, 401 Moraxella catarrhalis isolates were obtained from respiratory tract in children. Minimum inhibitory concentrations (MIC) of commonly-used antibiotics were determined by microbroth dilution assay, and beta-lactamase production was detected by Nitroceifn disk test. PCR combining restriction endonuclease analysis was employed to do the BRO genotyping. Results 96.5%iso-lates were beta-lactamase positive (387/401), MIC (MIC50/MIC90) values and resistant rates of beta-lactamase producing isolates were higher than those of non beta-lactamase producing isolates for ampicillin, cefaclor and cefuroxime (P<0.05). The positive rate of BRO gene was 99.2%in beta-lactamase producing isolates (384/387), consisting of 93.0%BRO-1 isolates and 7.0%BRO-2 isolates. MIC50 and MIC90 values of BRO-1+isolates were higher than those of BRO-2+isolates for ampicillin, cefaclor, cefuroxime and azithromycin. Conclusions The beta-lactamase production rate is high in Moraxella catarrhalis isolates from respiratory tract in children. BRO-1 type was the dominant genotype of beta-lactamase producing isolates, having more inlfuence than BRO-2 type in the inlfuence on some beta-lactams and macrolides.

Objective To investigate p38 MAPK signal transduction pathway of cytokine release in co-culture of BEAS-2B cells and eosinophils.Methods Eosinophils in human peripheral blood were isolated using anti-CD16 magnetic micro beads.Co-culture of BEAS-2B cells and eosinophils was taken as experimental model to investigate the inhibitive effect of SB 203580,a selective inhibitor of p38 MAPK,on release of IL-6,IL-8,IP-10 and MIG in culture supernatant.IL-6 was determined by ELISA kit and IL-8,IP-10 and MIG were measured using cytometric bead array(CBA)kit.Results SB 203580 could inhibit IL-6 and IL-8 release from BEAS-2B cells(P

Obsjective To investigate the effect of signal transduction pathway of p38 mitogen-activated protein kinase(p38MAPK) in epithelial cells activated by eosinophils on the expression and release of inflammatory mediators during co-culturing of two types of cells.Methods Eosinophils were isolated from human peripheral blood with magnetic micro beads conjugated CD16 antibody,LS separation column and magnetic cell sorting system(MCSS);The proteins of phospho-p38 MAPK and phospho-ATF-2 in BEAS-2B cells were analyzed by Western blot and enzyme-linked immuno sorbent assay(ELISA);The activity of phospho-p38MAPK was assayed by micro-bead Immunoprecipitatioin assay and Western blot.Results The interaction of eosinophils and BEAS-2B cells in co-culturing was found to elevate the protein expression and the activity of phospho-p38 MAPK in BEAS-2B cells;Phosphorylation of ATF-2 protein,a transcription factor that could regulate genes to express in cells and a substrate of phospho-p38 MAPK in the cascades of p38 MAPK signal transduction pathway was increased and it could be effectively suppressed by SB 203580,a selective inhibitor of p38 MAPK;eosinophils fixed with paraformaldehyde could also activate BEAS-2B cells to increase the phosphorylation of p38 MAPK protein.Conclusion To summarize the above results,it is showed that BEAS-2B cells activated by co-culturing with eosinophils to express and release inflammatory mediators are,at least partly,regulated by p38 MAPK signal transduction pathway.

Objective To investigate the value of the flow cytometry(FCM)for detecting platelet associated antibody in the di-agnosis of immune thrombocytopenia(ITP).Methods Platelet associated antibody,expression rate and the fluorescence intensity of platelet-associated immunoglobulin(PAIg)were measured in 51 patients with ITP(23 cases of newly diagnosed ITP and 28 cases of persistent ITP),21 patients with non-ITP and 60 healthy individuals.The correlation between the detection results with the platelet and megakaryocyte counts was performed;the expression rates of PAIg before and after treatment were compared.Results The fluorescence positive percentage and mean fluorescence intensity of IgG,Ig A and IgM in the patients group were significantly high-er than those in the control group with statistical difference(P <0.01).Compared with the non-ITP group,the difference had statis-tical significance(P <0.05).Compared with the autoimmune disease group,the difference had no statistical significance(P >0.05);the difference between the autoimmune disease group and the healthy control group had statistical significance(P <0.01 );in the persistent ITP group,PAIg in the complete response group had statistical difference between the groups before treament.PAIg in the response group also had statistical difference between the groups before treament (P <0.05).Conclusion FCM for detecting PAIg can be used in the diagnosis,differential diagnosis and the disease condition monitoring of ITP.

With the technology progress of human genome sequencing and biomedical analysis and the emergence of big data analysis tools,the new concept of precision medicine has been put forward.Clinically,the application of precision medicine becomes more and more widely in personalized medicine,genetic disease analysis and disease prediction,etc,which will be the trend of disease diagnosis and treatment in the future,however,precision medicine is based on the precise detection.The requirement of the precise detection promotes the rapid development of new detection methods.Clinical laboratory is the carrier to achieve accurate detection,we need to strengthen the construction of clinical laboratory and improve the management level of the laboratory,so as to achieve accurate detection,to provide a more effective basis for the diagnosis and treatment of precision medicine.

Objective Puerarin can remove inflammatory mediators, but the underlying mechanisms are not yet clear.The purpose of this study is to observe the synthesis of IL-6 in co-cultured bronchial epithelial (Beas-2B) and neutrophil cells, and to investigate the inhibitory effect of puerarin on IL-6.Methods Beas-2B and neutrophil cells were extracted and divided into six groups: neutrophil cells cultured alone (NC), Beas-2B cells cultured alone (BC), Beas-2B and neutrophil cells co-cultured (BC+NC), and Beas-2B and neutrophil cells co-cultured with puerarin at 50 μg/mL (BC+NC+P50), 100 μg/mL (BC+NC+P100) and 200 μg/mL (BC+NC+P200).The concentrations of IL-6 in different groups were quantitatively measured by ELISA and the expression of the IL-6 gene detected by real-time PCR.Results After 18 hours of culturing, the concentration of IL-6 was significantly increased in the BC+NC group ([280.409±21.340] pg/mL) as compared with those in BC ([240.002±23.727] pg/mL), NC ([22.771±1.146] pg/mL), BC+NC+P50 ([244.205±9.335] pg/mL), BC+NC+P100 ([218.168±18.635] pg/mL), and BC+NC+P200 ([179.539±7.340] pg/mL) (P<0.05), but lower in the BC+NC+P200 than in the BC+NC+P50 and BC+NC+P100 groups (P<0.05).The expression of the IL-6 gene was markedly up-regulated in the BC+NC group (1.515±0.013) in comparison with those in BC (1.000±0.215), BC+NC+P50 (0.398±0.024), BC+NC+P100 (0.332±0.016), and BC+NC+P200 (0.306±0.015) (P<0.05), but lower in the BC+NC+P200 than in the BC and BC+NC+P50 groups (P<0.05).Conclusion Direct-contact co-culture of Beas-2B and neutrophil cells significantly enhances the synthesis and release of the inflammatory mediator IL-6, which can be inhibited by puerarin.