1.Research on current education situation and demand of network teaching for health service management course
Chinese Journal of Medical Education Research 2013;(6):561-563
Objective To explore the current education situation and demand of network teaching for health service management course so as to provide recommendations for improvement.Methods Questionnaire survey was conducted among 232 undergraduates from majors of preventive medicine,stomatology and clinical medicine.Totally 204 valid questionnaires were organized and recorded into database and SPSS 12.0 was employed to make descriptive statistical analysis of the data.Statistical results were used to show the current education situation and demand of network teaching for health service management course.Results 52.94% (108/204) of the students interviewed were dissatisfied with current teaching mode.87.25% (178/204) of the students believed the course website would be beneficial for learning.For the construction of the course website,health insurance system,health policy analysis and health organization system were considered both important and difficult.59.31% (121/204) of the students showed that the course website should be updated at least once a week.Conclusions It is necessary to explore the network teaching mode for health service management course.Teaching reform depending on different professions and curriculums should be carried out for network teaching.
4.RELATIVE BIOLOGICAL AVAILABILITY OF IRON IN THE BLOOD AND THE LIVER PASTE OF SWINE
Chengyu HUANG ; Maoyu ZHANG ; Shusheng PEAG
Acta Nutrimenta Sinica 1956;0(04):-
In the present work the relative biological availability (RBA) of iron in the blood and the liver paste of swine has been studied by means of the hemoglobin regeneration technique in chickens. One day-old chickens were first made anemic on a low iron basal diet for 3 weeks, and were then divided into groups to which basal diets with various iron levels (10, 20, 40 ppm Fe) from different iron sources (ferrous sulfate reference standard, whole blood meal of swine and liver paste of swine) were subsequently fed respectively.After 16 days on test diets hemoglobin levels and hematocrit values in the blood of the chickens were measured. The chickens were then sacrificed for measurement of the iron concentration in their livers.Regression lines of each iron source were fitted by plotting final hemo- globin concentrations against the corresponding iron concentrations of the diet fed. Let the slope of the regression line from ferrous sulfate be 100, (i.e. relative bioavailability of ferrous sulfate), the RBA of the other 3 iron sources was as follows; processed whole blood meal of swine was 90.2; raw blood meal of swine 64.6; and the live paste of swine 78.4; but the differences between any two of them are statistically non-significant; i.e. their effects on hemoglobin regeneration are of the same extent.
5.THE INFLUENCE OF KONJAC FOOD ON HUMAN LIPID METABOLISM
Maoyu ZHANG ; Chengyu HUANG ; Xu WANG ; Junrong HONG ; Shusheng PENG
Acta Nutrimenta Sinica 1956;0(01):-
With food containing konjac flour, a 45-day-long feeding trial on 110 old people suffering from hyperlipidemia was conducted to observe the effect of konjac food on lipid metabolism of human beings.The konjac flour was from Amorphophallus konjac, a kind of traditional food, with trace protein and very low energy, but rich in dietary fibre composed mainly of gluco-mannan.The results obtained were as follows;1.After having konjac flour 5g/day as a supplement of their regular meal for 45 days, concentration of TG, TC and LDLC in the serum of the experimental subjects decreased significantly (P
6.STUDY ON COLONIC MICROFLORA IN LAC TOSE INTOLERANTS WITH FLUORESCENCE IN SITU HY BRIDIZATION
Yan ZHONG ; Chengyu HUANG ; Rj VONK ; Hmj HARMSEN
Acta Nutrimenta Sinica 1956;0(04):-
Objective: To study the relationship between colonic microflora and lactose intolerance symptoms. Methods: 42 volunteers were chosen and divided into lactose intolerance(LI) and lactose malabsorption groups according to the 25 g lactose-challenge test and symptoms questionnaires. Fresh fecal samples were collected from the volunteers, and the fluorescence in situ hybridization (FISH) technique with group-specific 16S rRNA-targeted oligonucleotide probes was used to quantify the total bacterial and predominant groups of bacteria in fecal samples. The effect of milk-drinking habit on the number of Bifidobacteria was analyzed. Results: It showed that there was significant difference in the number of total bacteria and Bifidobacteria between lactose intolerance groups and malabsorption group(P
7.EFFECTS OF ANIMAL BLOOD AND LIVER ON THE STATUS OF IRON NUTRITION IN PRESCHOOL CHILDREN
Chengyu HUANG ; Maoyu ZHANG ; Junrong HONG ; Shusheng PENG ;
Acta Nutrimenta Sinica 1956;0(02):-
In the present article the effects of animal blood and liver on the status of iron nutrition in preschool children were studied.71 apparently normal children in a kindergarten, aged 3-7 years, were selected for feeding trial. All of them were undergone physical examination to verify the absence of hookworm infestation and chronic diseases, but having a bit lower hemoglobin concentration (Hb
8.EFFECTS OF VITAMIN B 1, C AND ORANGE JUICE ON HUMAN LUNG CANCER CELLS IN VITRO
Xiaona WU ; Chengyu HUANG ; Yongtao YANG ; Tao HE ;
Acta Nutrimenta Sinica 1956;0(03):-
Objective: To investigate the effects of vitamin B 1, C and orange juice on human lung cancer cell proliferation in vitro. Methods: Vitamin C degradation in culture medium was evaluated. The methods included: methy thiazolyl tetrazolium (MTT) assay, colony forming assay and 3H TdR incorporation test. The final concentrations of factors in medium were: orange juice (vitamin C 30,60,120 ?g/ml), vitamin C(30,60,120 ?g/ml), vitamin B 1 (0.01,0.1,1 ?g/ml). Results: (1) In MTT test, orange juice at each level had significant inhibitory effect on the growth of lung cancer cells (P
9.DETERMINATION OF VITAMIN C IN SERUM BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Xiaona WU ; Chengyu HUANG ; Aimin SUN ; Yongtao YANG ;
Acta Nutrimenta Sinica 1956;0(03):-
Objective: To assay the content and stability of vitamin C(VC) in serum by a simple, specific method. Methods: Serum samples were extracted by metaphosphoric acid, and analyzed on a column C 18 of HPLC with DAD detector at 234 nm. Mobile phase was 100 mmol/L KH 2PO 4, pH3.00. The flow rate was 1 ml/min. Results: Under this condition, the retention time of VC was 5.76 min. The within run and between run reproducibilities tests were 0.42% and 2.11% respectively, and the recovery rate ranged from 82% to 88.5%. Stored at -70 ℃ and compared to that of its metaphosphoric acid extract, serum VC content was stable only for two days, and then decreased rapidly, whereas it did not decrease in metaphosphoric acid extract until the fifth day. Conclusion: A simple, sensitive and reliable method of HPLC with DAD detector is established for measuring VC in either serum or fruit, such as orange juice. VC should be determined immediately or should be extracted with metaphosphoric acid as soon as possible, then the extracts are kept at -70 ℃, and analysed within 5 d.
10.ASSESSMENT OF IRON BIOAVAILABILITY OF IRON BIOFORTIFIED MAIZE USING IN VITRO DIGESTION/CACO-2 CELL CULTURE MODEL
Mingqiu ZHANG ; Kangning WANG ; Ji LEI ; Xiangfeng YUE ; Chuanxiao XIE ; Chengyu HUANG
Acta Nutrimenta Sinica 2004;0(06):-
Objective To assess iron bioavailability of three iron-biofortified maizes using an in vitro digestion/ Caco-2 cell culture model. Method Three maize varieties rich in iron (Zhongtie 2, 3 and 4) and two maize varieties with lower content of iron (Zhengda 818 and 619) were pulverized, and the content of iron and phosphorus in each maize variety was measured. After digestion the iron bioavailability per g food was determined by using Caco-2 cell ferritin formation per mg cell protein as indicator. Results The iron content (33.250 mg/kg), iron bioavailability and the iron bioavailability per g food of Zhongtie 2 maize was the highest among all varieties, but its phosphorus content was lower. The maize varieties with the next higher iron bioavailability were Zhongtie 3 and Zhengda 818, and the lowest was Zhengda 619. Conclusion Zhongtie 2 maize contained the hightest content and bioavailability of iron,so it was optimal to use for the following human trials.