1.Effects of urokinase -type plasminogen activator on high glucose-induced rat mesangial cells proliferation and phenotype transformation
Hui CHENG ; Guohua DING ; Cheng CHEN ; Ming SHI ; Hongxia YANG
Chinese Journal of Nephrology 2011;27(7):515-519
Objective To explore the effects and mechanisms of urokinase-type plasminogen activator (uPA) on high glucose-induced rat mesangial cells proliferation and phenotype transformation. Methods Rat mesangial cells were cultured and incubated in media containing either 5 mmol/L D-glucose or 30 mmol/L D-glucose with or without addition of wortmannin, or uPA (105 U/L) for different time periods. At the end of the incubation period, mesangial cells proliferation was assessed by MTT assay and flow cytometric analysis. Cyclin-dependent kinase 2 (CDK2) and p27kip1 expression and activation of Akt were evaluated by Western blotting and Akt kinase assay respectively. Furthermore, the expression and distribution of α-SMA were detected with laser confocal microscopy. Results MTT assay and flow cytometric analysis demonstrated that high glucose induced mesangial cells proliferation (P<0.05) and an incresed proportion of cells in G2/M+S stage after 24 h incubation (P<0.01), which were attenuated by uPA or wortmannin (P<0.01). High glucose induced the enhance of Akt activity after 3 h (P<0.05), and the effect was inhibited by wortmannin or uPA (P<0.01). High glucose did not alter CDK2 expression (P>0.05),but significantly inhibited p27kip1 expression (P<0.05), which was attenuated by wortmannin or uPA (P<0.01). High glucose induced the up-regulation of α-SMA expression and perinucleus location in mesangial cells after 24 h (P<0.01), which were alleviated by wortmannin or uPA (P<0.01). Conclusion uPA up-regulates p27kip1 expression and counteracts high glucose-induced mesangial cells proliferation and phenotype transformation via blocking PI3K-Akt signaling pathway.
2.Comparison of decellularizative procedures of bovine pericardium tissue-engineering heart valve scaffold
Min YANG ; Changzhi CHEN ; Shaofei CHENG
Chinese Journal of Thoracic and Cardiovascular Surgery 2003;0(06):-
Objective Compared with different decellularization procedures for their potential of cell removal and the ability to preserve the matrix. Methods Specimens of bovine pericardiums were treated by 3 approaches (detergent and enzyme extraction、trypsin 、Triton-X 100 and sodium-deoxycholate). Tissue samples were then observed by HE staining and scanning electron microscopy to confirm the removal of cells. Von Gieson(VG) staining and Gomori staining were used for showing the integrity of collagen and elastin. DNA content was examined by the method of DNA extraction. Tissue shrinkage temperature and mechanical properties were also studied. Results Completely decellularization were achieved in 3 groups. While trypsin、Triton-X 100 and sodium-deoxycholate caused severe structural destruction and declined of mechanical properties of the matrix. In contrast, detergent and enzyme extraction achieved completely deeellularization and effectively preservation the matrix structure. Conclusion This research demonstrated detergent and enzyme extraction could achieve both complete decellularization and preservation of the matrix structure. This approach may provide an ideal platform for the construction of tissue-engineering heart valves.
3.Screening of binding protein of surface protein promoter Ⅱ DNA of hepatitis B virus by phage display from human liver cDNA library
Yanjie YANG ; Jun CHENG ; Dongfeng CHEN
Medical Journal of Chinese People's Liberation Army 1982;0(01):-
Objective To screen the HBV SPⅡ promoter DNA-binding protein, and to investigate its potential role in the regulation of replication and expression of HBV DNA. Methods By using HBV SPⅡ biotinylated promoter DNA as a selective molecule, the T7 select human liver cDNA library was biopanned and the positive clones were selected. After screening, amplification of positive plaques was performed for inserted DNA fragment and then they were cloned into the pGEM-Teasy vector. Results Four positive plaques were chosen for DNA sequencing. The binding protein of HBV SPⅡ promoter was demonstrated as nicotinamide adenine dinucleotide (NADH) dehydrogenase 4 by BLAST. Conclusion The result suggests that this approach may provide a new tool for the study of replication and expression mechanism of HBV DNA.
4.Current Strategies for Polypeptide Fusion Tags Removal
Hao XIE ; Cheng YANG ; Lie CHEN
Progress in Biochemistry and Biophysics 2006;0(10):-
Using gene fusion technology, polypeptide fusion tags can be engineered into target proteins at the genetic level.The resultant recombinant proteins may possess the biochemical properties of the imported fusion tags.Therefore, it is possible to take advantage of fusion tags to improve and evaluate protein expression, to detect and track protein targets, and to purify and characterize proteins.However, it is necessary to eliminate any influence of the fusion tag in structural characterization experiments or in isolating pharmaceutical proteins.Scientists must therefore remove fusion tags prior to structural and functional analyses when fusion tags are suspected of interfering with the biological activity of a protein or influencing its behavior.The fusion tag can be removed by several methods including harsh chemical treatment, mild enzymatic cleavage by endoprotease or exoprotease, and intein-mediated self-cleavage.Here the literature is reviewed in relation to principles, applications, and approaches of each method.
5.Pharmacokinetics and Safety of Drugs in Mother and Fetus During Pregnancy
Yong YANG ; Cheng CHEN ; Xinxia LIU
Herald of Medicine 2017;36(9):951-955
In order to favor the growth and development of the fetus,the maternal-placental-fetal unit is characterized by profound physiologic changes.These adaptations may affect maternal and fetal absorption,distribution,placental transfer,metabolism and excretion of drugs.When evaluating drug use in pregnancy,understanding both pregnancy physiology and the gestation-specific pharmacokinetics of different drugs is necessary to achieve effective treatment and limit maternal and fetal risk.This topic will focus on the pharmacokinetics and safety of drugs during pregnancy in light of changes in maternal physiology and function of placental.
6.STUDY ON RELATIONSHIP BETWEEN ras, p16, p53, HELICOBACTER PYLORI AND GASTRIC CARCINOMA
Lixin CHEN ; Liufang CHENG ; Yunshen YANG
Medical Journal of Chinese People's Liberation Army 1982;0(03):-
Biopsies were randomly taken from 160 patients through endoscopy. Among them 46 patients suffered from chronic superfacial gastritis, 39 patients from chronic atrophic gastritis, 39 patients from gastric ulcer, and 36 patients from gastric cancer. Ras, p16, and p53 genes were analysed with polymerase chain reaction single strand conformation polymorphism (PCR SSCP). Helicobacter pylori (HP) was examined with RUT. Result: ① the positive rates of ras and mutant p53 in gastric cancer were significantly higher than that in gastritis and gastric ulcer( P
7.Development of ZJ-XD-I multifunctional internal sterilization machine for medical instrumentation
Ning CHEN ; Cheng YANG ; Zhengying FU ; Lin QU ; Fan CHEN
Chinese Medical Equipment Journal 1989;0(03):-
ZJ-XD-I multifunctional internal sterilization machine for medical instrumentation is applied in medical instrumentations with circulatory loop such as anesthetic machine and respirator. The machine can generate ozone and hydrogen peroxide, and then the two gases are mixed and introduced into the internal loop to eliminate adnexed hydrogen peroxide such as the virus and germ, and thus the nosocomial infection due to repeated uses of medical instrumentations. The machine is composed of the gas compression component, atomization unit, gas extraction component, control circuit and the filtrating unit.
8.Midterm outcome of a half-blind, random, control research : a new therapy that treat hypovascular liver cancer with balloon catheter to block the main artery temporarily, and then pressurize chemoembolization
Yongbo YANG ; Hongyan CHENG ; Liang ZHANG ; Yufang CHEN
Chinese Journal of Radiology 2012;46(8):724-729
Objective To investigate efficacy and effect on liver function of the experimental therapy with balloon catheter to block the main artery temporarily and then pressurize chemoembolization to treat hypovascular liver cancer. Methods Eighty patients with hypovascular liver cancer requiring interventional therapy were randomly divided into two groups.The experimental group was treated with the new therapy and the control group was treated with traditional therapy. The lipiodol-filling status and maximum diameter of the tumor was analyzed for a midterm outcome,and the change of AFP and liver function were evaluated.Mann-Whitney test was used for data between two groups,Friedman test was used for data of each group,and Spearman nonparameter relevant analysis was used for efficacy indexes.Results ( 1 ) All Patients have confirmed diagnosis of hypocvascular liver cancer and got balanced baseline. ( 2 ) Lipiodol-filling status:the clinical efficacy and benefit rates of patients from experimental group were higher than that from control group and showed statistically significant difference in 1,3,12 months (Z =-2.135,- 2.939,- 2.686 ; P =0.034,0.004,0.007 ),but no statistically significant difference in 6 month ( Z =- 1.170,P =0.242 ).The status of lipiodol-filling of experimental group ( x2 =2.593,P =0.459 ) was more stable than control group ( x2 =10.886,P =0.012).(3) Maximum diameter of the tumor:the clinical efficacy and benefit rates of patients from experimental group were higher than that from control group and showed statistically significant difference in 3,12 months ( Z =- 2.734,- 2.733 ; P =0.006,0.006),but no statistically significant difference in 1,6 month ( Z =- 1.692,- 1.895 ;P =0.091,0.058). But neither of two groups showed statistically significant difference in change of maximum diameter of the tumor ( x2 =5.500,P =0.139 ; x2 =6.509,P =0.089 ).Relation between lipiodol-filling and maximum diameter showed positive correlation in 3 month ( r =0.257,P =0.035 ). (4) Stratified analysis was used for data of AFP according to AFP value before therapy,and two groups showed no statistically significant difference for patients belonging to 20-1000 μg/L by Pearson Chi-square test. (5)Two groups showed no statistically significant difference for data of liver function before therapy and in 1,3,6months ( Z =- 1.073,- 1.314,-0.518,-0.549;P=0.308,0.189,0.604,0.583).Conclusions According to the midterm result of this experiment,the experimental therapy increased lipiodol-filling and decreased maximum diameter of the tumor significantly in 3 and 12 months correspondingly,but no significant difference was observed in AFP and liver function between groups yet. So the long-term efficacy and its influence to lung metastasis and survival rate need further research.
9.Diagnosis and treatment of primary small bowel tumors
Yongqiang WENG ; Aiqun CHENG ; Jie ZHU ; Haobin CHEN ; Jianying YANG
Chinese Journal of General Surgery 2001;10(3):253-255
Objective To study the clinical manifestation of small bowel tumors and the prognostic factors. Methods Retrospective analysis of the clinical data were made on 42 cases of primary small bowel tumors (except periampullary tumors) admitted to our hospital from 1974 to 1999. Results In this series, there were 6 cases of benign small bowel tumors, 5 of them were leiomyoma and 1 adenoma. Of the 36 cases of malignant tumors, there were malignant lyphoma in 17 cases (47.2%), adenocarcinoma in 11 (30.6%), leiomyosarcoma in 6(16.7%), carcinoid tumor in 1 (2.8%) and neurofibrosarcoma in 1 (2.8%). There was no characteristic clinical sign in small bowel tumors; endoscopy and barium series were the main diagnostic methods. About 50 per cent of patients with malignancy had metastasis, and only 18 cases (50%) received radical resection. Follow-up were available on 36 cases. The overall 5-year survival rate in the patients with malignant small bowel tumors was 47.3%. Multivariate analysis showed that metastasis and diameter of tumors were the main prognostic factors of the 5-year survival. Conclusions Early diagnosis and early treatment play the key roles in improving the prognosis of malignant small bowel tumors. Radical resection should be taken if no metastasis is found.
10.Progress in the study of tubulin inhibitors.
Hai SHANG ; Li PAN ; Shu YANG ; Hong CHEN ; Maosheng CHENG
Acta Pharmaceutica Sinica 2010;45(9):1078-88
Microtubule is one of the key components of the cytoskeleton and plays an important role in the maintenance of cell shape and the process of signal transduction and mitosis. Due to the extreme importance of microtubule in the process of mitosis, tubulin becomes one of the most important targets for development of new anticancer drugs and tubulin inhibitors are used for the treatment of cancer nowadays. These inhibitors have antitumor activity by inhibiting or promoting the assembly of tubulin to microtubules and interfering the process of cell mitosis. This review summarized the research progress of the tubulin inhibitors, especially the introduction of the tubulin inhibitors of pharmacological activities and the progress of clinical research. Also, the development trend of these inhibitors is discussed.