Objective To explore the effect of gastrodia elata on learning and memory abilities and synaptic transmission protein(P38,Ca2+ -CaMK Ⅱ α,CREB)expression of hippocampus in model rats with Alzheimer's disease(AD).Methods 24 adult Wistar rats were randomly divided into control group,test group and intervention group.A dose of Aβ1-40 was injected into the hippocampus of rats on test group and intervention group,the control rats were injected with normal saline.When the models were successful,the rats of control group and test groups were given with sodium carboxymethyl cellulose(50g/kg),the rats of intervention group were given with gastrodia elata powder( 50 g/kg),lasting 15 days,Morris water maze test was used for learning and memory abilities study.The expression of P38,Ca2+-CaMK Ⅱ α and CREB protein were detected by immunohistochemistry method.Results Behavioral tests showed the mean escape latencies and search time of test group were obviously increased than those of control group and intervention group,the percentage of search distance on test groups was less than that of control group and intervention group(P ＜ 0.01 ).Immunohistochemistry results showed P38,Ca2+- CaMK Ⅱ α and CREB positive cells and optical density in hippocampus CA1 on test group were less than those of control group and intervention group ( all P ＜ 0.01 ) ( P38:58.92 ± 10.82,0.208 ± 0.037 ; Ca2+-CaMK Ⅱ α:72.38 ± 14.67,0.174 ± 0.036 ; CREB:53.86 ±5.31,0.161 ±0.043 in test group;P38:87.32 ±9.56,0.371 ±0.046 ; Ca2+ -CaMK Ⅱ α:98.16 ± 16.29,0.283 ± 0.051 ; CREB:86.76 ± 7.73,0.356 ± 0.052 in intervention group; P38:102.54 ± 16.73,0.563 ± 0.078 ; Ca2 + -CaMK Ⅱ α:123.46 ± 17.65,0.436 ± 0.057 ; CREB:125.43 ±9.16,0.524 ± 0.057 in control group ).Conclusion Gastrodia elata can treat AD by increasing expression of P38,Ca2 + -CaMK Ⅱ α and CREB.

Objective To explore the relationship between the expression level of p27 KIP1 and p57 KIP2 and the development and prognosis of thyroid carcinoma. Methods p27 KIP1 and p57 KIP2 expression in 6 nodular goitres, 10 thyroid adenomas and 35 thyroid carcinoma were detected by immunohistochemical method, and the relationship between p27 KIP1 and p57 KIP2 expression and clinicopathological characteristics were analyzed. Results The positive rate of nuclear expression of p27 KIP1 and p57 KIP2 in nodular goitres and thyroid adenomas was obviously higher than that in thyroid carcinomas, in papillary carcinoma and follicular carcinoma was significantly higher than that in medullary carcinoma and undifferentiated carcinoma, and in the thyroid carcinomas of stages I and II was also significantly higher that in the thyroid carcinomas of stages III and IV. The frequency of cytoplasmic expression of both p27 KIP1 and p57 KIP2 in thyroid carcinoma with lymph node metastasis was obviously higher than that in ones without lymph node metastasis. Conclusion The nuclear expression of both p27 KIP1 and p57 KIP2 in thyroid carcinoma were significantly associated with clinical stage, lymph node metastasis and local invasion. The low nuclear expression of p27 KIP1 and p57 KIP2 may indicate the poor prognosis of the patients, and the cytoplasmic expression may indicate the lymph node metastasis.

Objective: To study the gender differences in ERPs recorded during a recognition task for Chinese words. Methods: 15 male and 15 female healthy volunteers received a Chinese words recognition task and the EEG signals were recorded from 20 scalp sites simultaneously. The stimuli were meaningful words consisting of two Chinese characters. Results: Both men and females showed a positive-going parietal old/new effect, but females demonstrated larger P500 amplitudes and shorter P500 latency than males. Furthermore, the parietal old/new effect of males was significantly left-lateralized, while that of females showed more bilateral pattern. Only males elicited obvious frontally distributed old/new effect between 250 and 350 ms, which was negative-going and right-lateralized. Conclusion: Gender differences do exist in the recognition processing of Chinese words. Some of the gender differences could be interpreted as reflecting different strengths and speed of brain activation and others could be interpreted as reflecting the involvement of different neural structures.

Administration, Inhalation ; Humans ; Respiratory Therapy

0.05).Conclusions RFA treatment of PHC patients does not lead to hematogenous tumor dissemination.

Cancer is considered as one of the major diseases endangering human health in the world, it is urgent to find a safer and more efficient treatment for cancer therapy. Gene therapy with ribonucleic acid (RNA) drugs could regulate the expression of tumor related genes, and exhibit good anti-tumor therapeutic potential in preclinical and clinical trials. Based on the differences between tumor tissues and normal tissues in microenvironment signal characteristics such as pH, specific enzyme concentration or redox gradient, various microenvironment responsive nanocarriers had been studied and developed to deliver RNA drugs to tumor tissues and cells, improving the anti-tumor efficacy of RNA drugs and reducing toxic and side effects. This paper reviews the pathophysiological characteristics of tumor microenvironment and various strategies of tumor microenvironment responsive nanocarriers, in order to provide reference for the design of safe and efficient RNA drug delivery system for cancer therapy.

Dentin-Bonding Agents ; Water

Parsed the ECG descriptions in DICOM 3.0 standard and accomplished a DICOM-ECG file which conforms to the DICOM standard by a toolkit DCMTK. The DICOM-ECG file can communicate with systems which support DICOM standard directly.
Electrocardiography ; instrumentation ; methods ; Information Storage and Retrieval ; Software

Objective To explore the features of high-frequency ultrasonography and contrast-enhanced ultrasonography (CEUS) of papillary thyroid microcarcinoma (PTMC).Methods The CEUS data and ultrasound data of 147 PTMCS which were reconfirmed by pathology were analyzed retrospectively,and the CEUS and ultrasonic characteristics of them were summarized.Results Among 147 nodules,144 (97.9％) nodules were hypoechoic,and 3 nodules were isoechoic.Vague edge was found in 136(92.5％) PTMCs,and 126(85.7％) PTMCs were irregular in shape.Totally 92(62.6％) PTMCs were A/T ＞ 1,microcalcifications were found in 81 (55.1％) PTMCs.Besides,26(74.2％) PTMCs were found microcalcification in 35 PTMCs combined with Hashimoto's thyroiditis (HT),while 55 (49.1％) PTMCs were found microcalcification in 112 PTMCs combined with HT.There were significant differences between them (P ＜ 0.05).The blood distribution of 129 (87.8％) nodules was type Ⅱ.The contrast-enhanced pattern of 147 (100.0％) PTMCs showed in-homogeneous enhancement in 144 (97.9％) nodules,hypoenhancement in 136(92.5％) nodules,and all the nodules without amicula.Conclusions The typical PTMCs are hypoechoic,irregular shapeand vague edge,usually were found as A/T ＞ 1,microcalcification,and type Ⅱ blood distribution.With the method of contrast-enhanced ultrasonography,these nodules usually without amicula showed inhomogeneous and hypoenhancement.The incidence of microcalcification is more common when patients with Hashimoto's disease coexisting PTMC.

Background and purpose:The theory of cancer stem cell offers us a new thought about tumors, more and more kinds of cancer stem cell were isolated and indentif ied from corresponding cancer tissue. Our aim was to investigate the content of side population cells in SW480 human colorectal cancer ce11 line and to enrich cancer stem- like cells in SW480 through serum-free medium (SFM) culture. Methods:The percentage of side population cells in human colorectal cancer cell line SW480 was detected with ? ow cytometry. SW480 cell line was cultivated in serum- free medium(SFM) supplemented with growth factors and the cancer stem-like cells reforming into ? oating spheres were isolated. The isolated cancer stem-like cells were identifi ed by limited-dilution assay, differentiation assay, self- renewal assay, and alternative cultivation assay. Results:The percentage of SP cells was 1.2% in SW480,In the absence of serum, a minority (0.54%-0.62%) of cancer stem-like cells in SW480 cells survived, proliferated and formed into the suspended tumor cell spheres. SW480 cancer stem-like cells possessed proliferative, self-renewal and differentiation potential, which were responsible for the ? oating tumor clone. Serum addition into SFM resulted in the proliferation of cancer stem-like cells; after several generations and alternated cultivation in SSM and SFM, cancer stem-like cells maintained their characteristics. Conclusions:SW480 cell line contains a tiny minority of SP cells with stem cell properties.The cancer stem-like cells in SW480 line can be maintained in SFM using a floating culture method.