1.Role of IL-6 induced human peripheral blood monocytes tissue factor expression in the pathogenesis of chronic rejection
Jun LI ; Mei HONG ; Tie-Cheng PAN ;
Chinese Journal of Organ Transplantation 2005;0(10):-
Objective To study the induction of IL-6 on expression and activity of tissue factor (TF)in peripheral blood monocytes(PBMCs).Methods rhIL-6 100 ng/L and rhIL-6 100 ng/L+ rhIL-6 MoAb 10?g/L were used respectively to stimulate the PBMCs for 24 h,PBMCs without any stimulation as control group,to study the changes of antigen content,mRNA expression and the ac- tivity of the TF.Results The antigen content,mRNA expression and activity of TF were increased significantly in 100 ng/L rhIL-6 group as compared with rhIL-6 100 ng/L+rhIL-6 MoAb 10?g/L and control groups(P<0.01).Conclusions rhIL-6 can induce the increase of antigen expression,activity and mRNA expression in PBMCs,and these effects can be suppressed successfully by rhIL-6 MoAb. This study demonstrated that there was potential relations between cytokines and thrombogenesis, which may play an important role the pathogenesis of chronic rejection.
2.Repair of ear deformity: a study of different methods
Xuwu WANG ; Xiuyun HU ; Hui LI ; Xiaohua CHENG ; Mei LI
Chinese Journal of Medical Aesthetics and Cosmetology 2001;0(05):-
Objective To evaluate the surgical effects and influencing factors of ear malformation.Methods 408 cases were reviewed,including surgical methods and its effect in different condition of malformation,in which autogenous rib cartilage was used in reconstruction of ear.Meanwhile,349 cases(401 ears) double-lobe skin flap and 29 cases(29 ears) retroauricular groove flap were designed according to different condition of malformation.All the patients were treated with canalplasty,and 84 cases(84 ears) with mastoid process of pneumatic type were treated with tympanoplasty and canalplasty.Results months and more follow-up obtained in 335 cases (82%),and 6 months to 5 years follow-up in 273 cases(67%).Surgical effect was satisfactory,the shape of reconstructive ear was verisimilitude,and skull auricle angle was suitability;hearing was improved after operation in 335 ears,especially in 84 ears with canalplasty and tympanoplasty,but hearing was not improved after operation in 45 ears(
3.Experience in the teaching of child neurologic training doctors
Min ZHONG ; Mei LI ; Min CHENG ; Siqi HONG ; Li JIANG
Chinese Journal of Medical Education Research 2011;10(7):891-893
Based on their own characteristics and learning purposes of the child neurologic training doctors and to full use of the advantage of our hospital in faculties, facilities and patients, the plan was designed and focused on better understanding of clinical related basic theoretical knowledge, improving the ability of clinical research and thesis writing, strengthening communication skills between patients and doctors, and medical ethics training and health regulations learning. The training doctors achieved good results in the aspects of medical skills, research ability and comprehensive professional quality.
4.An analysis of hospitalization rates of patients with goiter in Lianyungang City Jiangsu Province in 2002-2010
Jian-mei, DONG ; Wei-wei, LI ; Xu-cheng, QIN
Chinese Journal of Endemiology 2013;32(4):419-423
Objective To observe the changes of hospitalization rates of patients with goiter in Lianyungang City in a coastal area with mild iodine deficiency in 2002-2010.Methods Medical reports of patients with goiter from county hospitals were studied at county level with a retrospective method.In accordance with the National Iodized Salt Monitoring Program,nine townships(towns) were taken from each county (district) [total of five counties(districts)]; four villages were selected from each township (town); salt samples were taken from eight households in each village,and salt iodine was determined using direct titration of sodium thiosulfate.Results The hospitalization rate of patient with goiter increased from 1.87/10 million in 2002 to 7.05/10 million in 2009,and then fluctuated to 6.06/10 million in 2010(x2 =281.91,P< 0.01).Among them,female's hospitalization rates were significantly higher than that of male 's(The variation range of x2 values were 35.23-116.04,all P < 0.01),and the hospitalization rates of people over the age of 40 years were significantly higher than that of people less than 40 years of age(The variation range of x2 values were 33.04-263.04,all P < 0.01),and the hospitalization rates of people in urban areas were significantly higher than that of people in rural areas (The variation range of x2 values were 18.35-140.00,all P < 0.01).Average salt iodine was 27.61-30.13 mg/kg in 2002-2010.The coverage rate of iodized salt and the consumption rate of qualified iodized salt both increased year by year (x2 =183.75,211.99,P < 0.01).There was a positive correlation between hospitalization rate of patient with goiter and coverage rate of iodized salt(spearman correlation coefficient was 0.83,P < 0.05).There was a positive correlation between hospitalization rate of patient with goiter and the consumption rate of qualified iodized salt too (spearman correlation coefficient was 0.93,P < 0.05).Conclusions In Lianyungang City,the hospitalization rate of patient with goiter,the coverage rate of iodized salt and the consumption rate of qualified iodized salt all show upward trend in 2002-2010.The relationship among them is worth further study.
5.Cloning and bioinformatic analysis and expression analysis of beta-glucuronidase in Scutellaria baicalensis.
Shuang-shuang GUO ; Lin CHENG ; Li-min YANG ; Mei HAN
China Journal of Chinese Materia Medica 2015;40(22):4370-4377
The β-Glucuronidase gene (sbGUS) cDNA firstly from Scutellari abaicalensis leaf was cloned by RT-PCR, with GenBank accession number KR364726. The full length cDNA of sbGUS was 1 584 bp with an open reading frame (ORF), encoding an unstable protein with 527 amino acids. The bioinformatic analysis showed that the sbGUS encoding protein had isoelectric point (pI) of 5.55 and a calculated molecular weight about 58.724 8 kDa, with a transmembrane regions and signal peptide, had conserved domains of glycoside hydrolase super family and unintegrated trans-glycosidase catalytic structure. In the secondary structure, the percentage of alpha helix, extended strand, β-extended and random coil were 25.62%, 28.84%, 13.28% and 32.26%, respectively. The homologous analysis indicated the nucleotide sequence 98.93% similarity and the amino acid sequence 98.29% similarity with S. baicalensis (BAA97804.1), in the nine positions were different. The expression level of sGUS was the highest in root based on a real-time PCR analysis, followed by flower and stem, and the lowest was in stem. The results provide a foundation for exploring the molecular function of sbGUS involved in baicalcin biosynthesis based on synthetic biology approach in S. baicalensis plants.
Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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Computational Biology
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Glucuronidase
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chemistry
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genetics
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metabolism
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Molecular Sequence Data
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Open Reading Frames
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Phylogeny
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Plant Proteins
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chemistry
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genetics
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metabolism
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Protein Structure, Secondary
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Scutellaria baicalensis
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chemistry
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enzymology
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genetics
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Sequence Alignment
6.Antibody titre quantitation method by ELISA without standard substance based on kinetics of enzyme-catalyzed reactions
Cheng FANG ; Zhuo CHEN ; Li LIU ; Zhiqiang MEI
Chongqing Medicine 2015;44(12):1662-1663,1666
Objective To establish antibody titre quantitation method by ELISwithoustandard substance .MethodThe tesgroupof non-specifi,negative control ,specifiand total antibodiewere sefodetecting serantibodieby ELIS.Aftelineafitting of the time-absorbance datof early developing ,the fitted slopewere used athe velocity of absorbance changing , which were denoted by ν0 ,νC,νS,νT.Based on thathe ν-value and the concentration ? of determinand were linearelationship while the substrate waexcessive ,the function with parameteof ν-valueforeflecting the multiple proportionof antibodiecon-centrationbetween specifiand negative control groupcould be deduced .The assessmenof specifiIgG antibodiein serof KM mice immunized with fish collagen waused aan instance .ResultThe function C/C= (ν-ν0 )/(νC-ν0 ) could calculate the mul-tiple proportion(titre) of antibodieconcentrationbetween specifiand negative control group.Conclusion The above method of antibody titre quantitation isuitable fosemi-quantitative analysiwithoustandard substance .
7.Survey on the knowledge and willingness of genetic counseling and testing in blood relatives of breast cancer patients
Xiaolin CHENG ; Zhengdong LI ; Xiaoyin SUN ; Zhangyi MEI ; Zhigang ZHUANG
Fudan University Journal of Medical Sciences 2017;44(3):312-318
Objective To investigate the knowledge and willingness of genetic counseling and testing in blood relatives of breast cancer patients.Methods A total of 922 blood relatives of breast cancer patients finished our questionnaire.Data were devided into different groups according to age,family history of tumor for statistical analysis.Results Most of the respondents were unaware of genetic counseling and genetic testing.However,after a brief introduction,major of them were willing to accept genetic counseling,breast cancer risk evaluation and screening.Specifically,79.8% of them were willing to accept genetic counseling,and 62.3% were willing to accept genetic testing.Most of the respondents would accept inexpensive early genetic screening.For the genetic testing with higher prices,only 37.9% of them would accept it.Supposing a positive genetic testing result,most of them were willing to perform prevention through close follow-upscreening,31.3% of them would choose prophylactic surgery or drugs.Despite being told the confidentiality of the test results,32.9% of them worried about the adverse effects of genetic test.Conclusions Most of the blood relatives of breast cancer patients were unaware of counseling and genetic testing,but had apparent willingness to accept them.Misunderstanding of genetic characteristics,costs and concerning about discrimination are obstacles for the respondents to accept genetic counseling,genetic testing and related screening prevention.
8.Effects of grape seed proanthocyanidin extracts on advanced glycation end product receptor and connective tissue growth factor in the myocardium of diabetic rats
Mei CHENG ; Baoying LI ; Qian WANG ; Ling XU ; Haiqing GAO
Chinese Journal of Geriatrics 2011;30(11):958-961
ObjectiveTo investigate the effects of grape seed proanthocyanidin extracts (GSPE) on advanced glycation end product receptor (RAGE),NF-Κb and connective tissue growth factor (CTGF) in the myoeardium of diabetic rats.MethodsTotal 30 streptozotocin (STZ) induced diabetic rats were randomly divided into 2 groups:diabetic group (DM1,n-15) and GSPE (250 mg/ kg,i.g) treated diabetic group (DM2,n= 15).Another two control groups:normal rats(C1,n= 10)and normal rats treated with GSPE (250 mg/kg,i.g) (C2 group,n= 10) were also observed.After 24 weeks,blood was collected to measure fasting plasma glucose (FBG) and RAGE.The protein expression of NF-Κb was determined in myocardial tissue by immunohistochemical staining and Western blot.The protein expressions of RAGE and CTGF were measured by Western blot.Results The levels of FBG and RAGE were significantly higher in diabetic rats than in control rats (P<0.05).After GSPE treatment,RAGE level significantly reduced (P<0.05),but FBG had no change in diabetic rats.The protein expressions of RAGE,NF-Κb and CTGF in the myocardial tissue of diabetic rats had marked increase compared with control rats (P< 0.05),however,their levels significantly reduced after GSPE treatment (P<0.05).ConclusionsGSPE may protect diabetic rats against cardiomyopathy,possibly by decreasing the protein expressions of RAGE,NF-Κb and CTGF.
9.Effects of ginkgolide (Gin) on cerebral water content, Na+, K(+) -ATPase activity, MDA, lactic acid of rats during acute hypoxia condition.
Jian-Cheng LI ; Shu-Yi JIN ; Xiao-Mei WU
Chinese Journal of Applied Physiology 2003;19(3):239-273
Animals
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Brain
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metabolism
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Female
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Ginkgolides
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pharmacology
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Hypoxia
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metabolism
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Lactic Acid
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metabolism
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Male
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Malondialdehyde
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metabolism
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Rats
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Rats, Sprague-Dawley
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Sodium-Potassium-Exchanging ATPase
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metabolism
10.The value of Gd-EOB-DTPA enhanced magnetic resonance imaging in the quantitative evaluation of liver function
Lili HUANG ; Zhibin CHENG ; Mei LI ; Yanli JIANG ; Jing ZHANG
Chinese Journal of Hepatobiliary Surgery 2014;20(5):324-327
Objective To explore the value of Gd-EOB-DTPA enhanced magnetic resonance imaging (MRI) in the quantitative evaluation of liver function.Methods Between June 2012 and June 2013,we prospectively included 8 patients with cirrhosis and 6 patients with primary hepatic carcinoma (PHC).The Child-Pugh classification,indocyanine green retention rate at 15 minutes (ICGR15),and Primovist enhanced MR imaging were performed in all the patients.Using the deconvolution method to calculate hepatic extraction fraction (HEF),the correlations between HEF and Child-Pugh and ICGR15 were analyzed.Results For cirrhosis,there was a significant correlation between the HEF and the ICGR15 by the Pearson correlation coefficient (r =-0.971,P < 0.05).For hepatocarcinoma,there was also a significant correlation (r =-0.855,P < 0.05).The average HEF of PHC was 21.80% (14.89% ~ 35.90%).Conclusions The Gd-EOB-DTPA enhanced MRI accurately evaluated liver function quantitatively.It may be used for patients with PHC for preoperative examination.