Since 2007,in order to comply with the hospital's strategic development and construction of the subjects,our hospital treat the research project management as the starting point,through the establishment of a sound project management and funding approaches; strengthen project process management,implement the total quality control; use of information technology tools to improve project management effectiveness.After several years of implementation,the number of our hospital's research projects above provincial and ministerial level has gradually increased,manyhigh level of SCI papers are emerging,and discipline construction has also been effectively carried out,all of which have promotedour research strength,the development of medical teaching and research.Therefore,we will continue to strengthen project management,and fully mobilize the initiative staff awareness of service,make full use of scientific information systems in future scientific research management to boost hospital disciplines.

Allergy and Immunology ; Ethnic Groups ; Humans ; Hypersensitivity ; immunology

Objective To investigate the employment pressure among graduates from nursing school,and explore influencing factors,in order to make suggestions on education management,career guidance and psychological intervention.Methods Students graduated from Nanyang Medical College in 2012 who majored in nursing and nursing graduates from Nanyang Institute of Technology were surveyed with medical students employment pressure questionnaire designed by DU Tian-jiao from China Medical University.SPSS13.0 was used to establish database and analyze data.Results The total employment pressure of all students was M=3.2.Seven stressors scores from high to level were:post requirements,social environment,family support,personal qualities,the school effect,professional requirements,and career guidance.Multiple regression analysis showed that whether or not the student leaders,different sources and the education background were the influencing factors of employment pressure.Conclusions This research suggests that different psychological intervention should be given to students majored in different specialty and sources in order to release employment pressure and anxiety.

The nonstructural protein 5A (NS5A) of the hepatitis C virus (HCV) has been shown to interact with a variety of cellular proteins and implicated in the regulation of cell growth, interferon resistance, and other cellular signaling pathways. Using the yeast-two hybrid method, we have isolated a clone that encodes a novel NS5A--associated binding protein: NS5ABP37. Reverse transcription polymerase chain reaction (RT-PCR) method was employed to amplify the full fragment,and the plasmid pGADT7-NS5ABP37 with the Saccharomyces cerevisiae vector pGADT7 was constructed. To prove the interaction, yeast cell Y187 transformed with pGADT7-NS5ABP37 was mated with yeast cell AH109 containing pGBKT7-NS5A to verify the interaction between the novel protein coded by the new gene NS5ABP37 and NS5A.

Objective HCV NS3 protein plays an important role in disease caused by HCV. We investigate the gene expression of HCV NS3 in yeast for future study of the function of the protein. Methods PCR was performed to amplify the gene of HCV NS3 from the plasmid pBRTM/HCV containing the whole fragment of HCV and the gene was cloned into pGEM T vector. Thereafter, HCV NS3 gene was cut from pGEM T vector and cloned into yeast expression plasmid pGBKT7, and recombinant pGBKT7∶NS3 was transformed into yeast AH109. The yeast protein was isolated and analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and Western blotting. Results HCV NS3 gene was successfully cloned into pGBKT7. The results of SDS PAGE and Western blotting assay showed that the molecular weight of the expressed product was about 22000 Da and HCV NS3 protein was existed within yeast cells.Conclusions HCV NS3 was successfully expressed in yeast expression system.

Protein protein binding is the basis of virus and host cell interactions. With the application of technology of studying of protein interactions, more knowledge of replication and pathogenesis of hepatitis C virus (HCV) could be acquired. Non structure protein NS5A is one of the important regulatory factors in virus replication , transcription and signal transduction, but there are controversy in effect on HCV pathogenesis and resistance to interferon ?(IFN?). In order to describe the relationship between NS5A and host proteins, we use yeast two hybrid system 3 to screen the gene encoding proteins that could interact with NS5A from human hepatocyte library. Thirty five clones were obtained including apo A1, apo A2, apo B100, haplotype mitochondrion complete genome, phosphatidic acid phosphatase type 2B, albumin similar to tumor endothelial marker 5 precursor, matrix metalloproteinase 14, and three of the Homo sapiens hypothetical proteins. The study paved a way for further studies on the pathogenesis of HCV NS5A

To clone the genes of hepatocyte protein interacting with hepatitis C virus NS3 protein, "bait" plasmids of hepatitis C virus NS3 were constructed. After verifying that hepatitis C virus NS3 protein could be steadily expressed in AH109 yeast strain, yeast two hybrid assay was berformed by mating AH109 with Y187 that pre transformed with liver cDNA library plasmids pACT2, and the diploidy yeast cells were plated on quadruple dropout (QDO) medium and assayed for X ? gal activity. Nineteen yeast colonies that could grow on QDO and had ? gal activity were obtained, then the library plasmids were extracted and sequenced. The gene sequences from the 19 positive colonies were aligned with the genes deposited in GenBank. It was found hepatitis C virus NS3 protein could interact with some proteins which have different functions.

Aged ; Anemia, Diamond-Blackfan ; complications ; Aza Compounds ; adverse effects ; Female ; Fluoroquinolones ; Humans ; Quinolines ; adverse effects ; Torsades de Pointes ; chemically induced ; complications

Objective To investigate the effect of ginsenoside Rg1 on apoptosis after spinal cord ischemia-reperfusion injury (SCII) in rats. Methods Forty-eight adult Sprague-Dawley rats were randomly divided into sham group (n=8), ischemia group (n=8), ischemia-reper-fusion group (n=16) and drug group (n=16). Fogarty catheter was put in the thoracic aorta of the rats and the blood flow wasn't blocked in the sham group. The rats in the ischemia group were sacrificed 30 minutes after spinal cord ischemia. The drug group was injected with gin-senoside Rg1 30 mg/kg 30 minutes before and after SCII. The same volume of normal saline was injected in the ischemia-reperfusion group at the same time. The expression of Bcl-2 and survivin was detected with immunohistochemistry at six hours, 24 hours after reperfusion in the ischemia-reperfusion group and drug group, 30 minutes after ischemia in the ischemia group and in the sham group. The change of cells was observed in each group with HE staining. Results The cells were damaged in the ischemia group, the ischemia-reperfusion group and the drug group, in which the drug group was better than the other groups. The expression of survivin and Bcl-2 was higher in the ischemia group, the ischemia-reperfusion group and the drug group than in the sham group (t>3.896, P<0.01), and were significantly higher six hours and 24 hours after reperfusion in the drug group than in the reperfusion group (t>6.693, P<0.001). Conclusion Ginsenoside Rg1 can reduce the neurons damage and increase the expression of the Bcl-2 and survivin, that inhibit cells apoptosis after SCII in rats.

To investigate different effects of fiscal health expenditure, household spending on health and social health expenditure on narrowing the gap between urban and rural health resource allocation. Methods: With the relevant data of China’ s medical and health through 1985-2011 years, taking methodology of the state space model to estimate the varying-time elasticity of different types of expenditures on urban and rural health resource allocation gap. Results: For narrowing the gap, household health expenditure played the leading role, fiscal health expenditure played smaller role and the social health expenditure played the supplementary role; the elastic of different health expenditure proportion was fluctuated before 2002, which became stable after 2002; it is easy to improve the “hard conditions” rather than the “soft conditions” . Conclusion: To accelerate the process of urban and rural medical security system integration, it is inevitable to establish an efficient configuration mechanism for urban and rural health expense, balanced develop urban and rural medical insurance system and scientifically guide social health investment.