The early diagnosis of breast cancer is crucial for improving the prognosis of patients.Some new diagnostic techniques can improve the early diagnosis rate.Studies show that microRNA,proteins in salivary,urinary polyamines could be used as novel tumor markers,with high sensitivities and specificities.With the help of optical technology and computers,the traditional diagnostic imaging technique also make a great breakthrough.Currently,image-guided minimally invasive biopsy techniques such as core needle biopsy and vacuum assisted biopsy have the advantages of accuracy,economy and little trauma,and can provide more samples,which have been widely applied in clinical practice.

OBJECTIVE:To evaluate the effect of adult pharmacy students in higher education on the teaching of professional courses in our school. METHODS:Questionnaire survey and Likert 5 point measurement method were used for the 2012-2014 grades of adult pharmacy students majoring pharmacy and drug management. RESULTS:Totally 260 questionnaires were sent out, and 236 were recyled with effective recovery of 90.8%. The average score of students for teaching attitude in professional courses was 3.58;the average score for teaching methods was 3.30;the average score for teaching ability satisfaction was 3.08;the aver-age satisfaction of students for teaching of professional courses was 78.98%;the average satisfaction of teaching attitudes,teaching methods and teaching abilities were 84.15%,78.13% and 76.65% respectively. CONCLUSIONS:The survey not only help estab-lishing a good interaction between students and teachers,promoting teaching and learing,but also play a role in monitoring and evaluating the teaching quality and effects of professional courses,which is conducive to promoting the process of teaching quality evaluation reform of adult higher learing.

It has been shown that powerful Saussurea involucrata spirit has effects of expelling wind and removing dampness, anti-inflammation and alleviating pain, and activating blood flow and diminishing swelling. The pharmacokinetic findings show that this drug has advantages of slow-absorption, long-action and high-bioavailability.

Epstein-Barr Virus Infections ; Humans ; Lymphoproliferative Disorders ; diagnosis ; genetics

Objective To observe the clinical therapeutic effect of Wenxintang in treating unstable angina pectoris. Methods Sixty patients of Yangqikuixu, Tanyuzuluo type suffered with unstable angina pectoris were randomly divided into two groups. 30 patients in the control group were treated with IsMo-20, Metoprolol and Aspirin, while another 30 patients in the treated group were treated with Wenxintang include the three medicines above. Each group was observed for 4 weeks respectively. Results The total effective rate in relieving angina in the treated group and the control group was 86.67% and 66.67% respectively, that in improving ECG changes was 69.23% and 47.82% respectively, the difference of the two parameters between the two groups was significant (P

Objective To reach parameter on sterilization wrap of medical instrument. Methods The parameter of one hundred twenty sterilization wraps with size 30 cm?40 cm?60 cm and weight 20kg was tested by Belimed sterilizer. Results There are 155 masculine gender and 445 negative in 600 quick reading biological indicators of 120 sterilization wraps and 53 masculine in bacteria detection, 9 cases failure in 1243 indicator, 52 wet wraps. Conclusion The parameter was to take three times negative pressure pulse and five times pressure pulse, sterilization temperature 134℃, sterilization time 11min and dry time 11min, matching to sterilization request of "Technical Standard For disinfection".

Objective To construct prokaryotic expression vector of NS5A transactivating protein 7 of hepatitis C virus (NS5ATP7), induce the expression of NS5ATP7 in E. coli, and to predict its structure and function by bioinformatics analysis. Methods NS5ATP7 gene was amplified by reverse transcription PCR (RT-PCR) using HepG2 cells mRNA as template, and was ligated into pGEM-T cloning vector. After verifying the sequence of the inserted fragment by restriction enzyme digestion identification and sequencing, NS5ATP7 was cloned into inducible prokaryotic expression vector pET-32a(+) and transfected into competent E. coli BL21. The protein expression was induced with IPTG and the product was analyzed by SDS-PAGE and Western blotting hybridization. The structure and function of NS5ATP7 were predicted using bioinformatics techniques. Results NS5ATP7 gene with about 891bp was amplified by RT-PCR, which was coincident with that we expected, and it was then inserted into expression vector pET-32a(+). Under the induction of IPTG, the recombinant NS5ATP7 was expressed successfully. SDS-PAGE and Western blotting assay showed that this recombinant protein possessed good immunogenicity. Bioinformatics method such as ExPASy, TMHMM and SignalP analysis indicated that NS5ATP7 was full of helices, had neither transmembranous structure nor signal peptide. Conclusions The recombinant NS5ATP7 gene could be successfully expressed in prokaryotic expression system of E. coli, which might lay the foundation of studying the immunogenicity and biological charactersitics of the NS5ATP7. Bioinformatics analysis may provide a new method to analyze the structure and function of a new protein.

Objective To clone the human gene of Hepatitis B virus e antigen binding protein 1 (HBEBP1), which was screened with yeast two-hybrid system and bioinformatics techniques, to construct prokaryotic expression vector of pET-32a(+)-HBEBP1, and to induce the expression of recombinant protein in E. coli BL21. Methods The DNA fragment of HBEBP1 of about 372bp was amplified by reverse transcription PCR (RT-PCR), in which the mRNA was taken from HepG2 cells as the template, and cloned into pGEM-T vector. After restriction enzyme digestion identification and sequencing, the correct target DNA fragment was inserted into inducible prokaryotic expression vector pET-32a(+) and then transformed into competent E. coli BL21. By restriction enzyme digestion and PCR, the positive transformed clones were identified and induced with IPTG to obtain fusion protein. The HBEBP1 fusion protein was analyzed by Western blotting hybridization. Results The 372bp DNA fragment of HBEBP1 was amplified by RT-PCR. The recombinant expression vector pET-32a(+)-HBEBP1 was constructed successfully. After transformation with pET-32a(+)-HBEBP1 and induction with IPTG, the recombinant target protein of about 33kD was obtained, which was consistent with our anticipation. Western blotting assay showed that the protein had good specificity. Conclusions The recombinant prokaryotic expression vector pET-32a(+)-HBEBP1 is constructed, and the HBEBP1 gene is cloned successfully. The HBEBP1 fusion protein could be expressed in prokaryotic expression system of E. coli. These results lays a foundative for studying the immunogenicity and the biological characteristics of the HBEBP1 protein.

Objective To study the interaction between HCV core protein and HCBP6 in mammlian cells using CheckMateTM Mammalian Two-Hybrid System.Methods cDNA fragments encoding HCV core protein and HCBP6 were amplified by PCR and subsequently cloned into pGEM-T vector.After verified by sequencing,the target fragments were subcloned into mammalian two-hybrid plasmids,pBIND and PACT,respectively.The recombinant plasmids,pBIND-core and pACT-HCBP6 were co-transfected into HepG2 cells with reporter plasmid pG5luc.pBIND+pACT were induced as background controls,pBIND-Myod+pACT-Id as positive controls,and pBIND-core+pACT,pBIND+pACT-HCBP6 as blank controls.The expression of G5luc,which indicated the interaction between HCV core protein and HCBP6 in mammlian HepG2 cells,was assayed through Dual-Luciferase Report Assay System and Turner Biosystems Veritas Microplate Lunimometer.Results The recombinant vectors pBIND-core and pACT-HCBP6 were successfully constructed.When co-transfected into HepG2 cells with reporter plasmid pG5luc,there were significant differences in the luciferase activity in the pBIND-core and pACT-HCBP6 groups compared with every control group.Conclusions HCBP6 can interact with HCV core protein in HepG2 cells,which provides clues for further study on the function of HCBP6 and core proteins,and on the mechanism of HCV core in cell apoptosis and cancer transformation.

Objective To know the drinking water quality in the rural areas in Shanxi province in order to enhance the management of drinking water and provide the scientific basis for water improvement in the rural areas. Methods 11 counties were selected based on the geographical condition in Shanxi province and 10 investigation sites were selected in each county based on the population proportion of different drinking-water types. 14 indicators were determined for every water sample. Results In the rural areas, most drinking water sources were ground water and the type of water supply was mainly non-central water supply. The indexes that exceeded the related standard limit were fluoride,arsenic and bacteria indicators. The hygienic state of central water supply was much better(?2=4.13, P