Objective To investigate the effect of naftopidil in the treatment of prostatitis in the elderly on MIP-2 and MIP-1αin serum and succus prostaticus.Methods 78 elderly patients with chronic prostatitis were randomly divided into the control group and the observation group, 39 cases in each group.The control group were treated with Diosmin routine treatment, the observation group were treated on the basis of the control group combined with naftopidil tablets in the treatment,2 groups were treated continuous for 8 weeks.MIP-2 and MIP-1αin serum and prostatic fluid before and after treatment were compared, and the maximum urine flow rate and clinical efficacy were compared after the treatment.Results compared with pre-treatment, MIP-2, MIP-1αin serum and prostate fluid in 2 groups after treatment decreased, NIH-CPSI and QOL scores in 2 groups decreased, the maximum urinary flow rate increased (P<0.05);compared with the control group, the levels of MIP-2, MIP-1αin the serum and prostatic fluid, NIH-CPSI and QOL of observation group were lower, and the maximum urine flow rate was higher (P<0.05);Compared with the control group, the total efficiency of the observation group was higher (P<0.05).Conclusion Naftopidil can significantly reduce the elderly patients with chronic non bacterial prostatitis and serum of patients with prostatic fluid MIP-2, MIP-1αlevel and improve pain in urination, dysuria symptoms.

In this study, the expression of IL-24 at maternal-fetal interface and the roles in extravillous trophoblast (the TEV-1 cell line) invasion were examined. Immunohistochemistry was used to detect the expression of IL-24 in villi and decidual tissue. The proliferation of TEV-1 cells under the effect of IL-24 was measured by MTT assay. The invasiveness of TEV-1 cells under the effect of recombinant IL-24 (rhIL-24) was examined by transwell system. Immunohistochemical detection showed that IL-24 was expressed in the villi and decidual tissue, and distributed in villous column, trophoblasts, stroma and blood vessels. The proliferation of TEV-1 cells was not inhibited by rhIL-24 of various concentrations. The examination of invasion in vitro showed that rhIL-24 could inhibit the invasion of TEV-1 cells in a concentration-dependent manner. The results suggested IL-24 could inhibit the invasion of TEV-1 cells. Therefore, IL-24 produced by maternal-fetal interface in human first trimester pregnancy may influence the invasion of trophoblasts and is involved in normal pregnancy.

Agriculture ; Humans ; Occupational Exposure ; adverse effects ; analysis ; Radiation Dosage ; Radon ; adverse effects ; analysis ; Seasons

Objective:To investigate the hepatitis C virus (HCV) infection in hemodialysis patients.Methods:One hundred and fifty hemodialysis patients were tested for HCV-RNA using a reverse transcription-polymerase chain reaction (RT-PCR) assay and for anti-HCV IgG using an enzyme-linked mmunosorbent assay (ELISA).Result:The positive rate of anti-HCV IgG was 24%.The positive rate of HCV-RNA was 26.7%;the total positive rate of HCV markers was 35.3%.Conclusion:HCV infection rate in hemodialysis patients is higher than that of general population.The first-class risk factors for HCV infection is transfusion of blood,while the cross using of dialyzer and dialysis pipe-line is also one of the risk factors.

Objective To construct prokaryotic expression vector of NS5A transactivating protein 7 of hepatitis C virus (NS5ATP7), induce the expression of NS5ATP7 in E. coli, and to predict its structure and function by bioinformatics analysis. Methods NS5ATP7 gene was amplified by reverse transcription PCR (RT-PCR) using HepG2 cells mRNA as template, and was ligated into pGEM-T cloning vector. After verifying the sequence of the inserted fragment by restriction enzyme digestion identification and sequencing, NS5ATP7 was cloned into inducible prokaryotic expression vector pET-32a(+) and transfected into competent E. coli BL21. The protein expression was induced with IPTG and the product was analyzed by SDS-PAGE and Western blotting hybridization. The structure and function of NS5ATP7 were predicted using bioinformatics techniques. Results NS5ATP7 gene with about 891bp was amplified by RT-PCR, which was coincident with that we expected, and it was then inserted into expression vector pET-32a(+). Under the induction of IPTG, the recombinant NS5ATP7 was expressed successfully. SDS-PAGE and Western blotting assay showed that this recombinant protein possessed good immunogenicity. Bioinformatics method such as ExPASy, TMHMM and SignalP analysis indicated that NS5ATP7 was full of helices, had neither transmembranous structure nor signal peptide. Conclusions The recombinant NS5ATP7 gene could be successfully expressed in prokaryotic expression system of E. coli, which might lay the foundation of studying the immunogenicity and biological charactersitics of the NS5ATP7. Bioinformatics analysis may provide a new method to analyze the structure and function of a new protein.

Objective To disclose the inter-species differences among Papaver somniferum L, Papaver rhoeas L and Cannabis sativa L using AFLP analysis. MethodsThe root, stem, foliage, flower, seed of Papaver somniferum L, foliage of Papaver rhoeas L and Cannabis sativa L were collected respectively. DNA was isolated with AxyPrep Kit, and double-digested by EcoR I and Mse I, then oligonucleotide adapters were ligated. After Pre-amplification, selective amplification was performed using 6 pairs of fluorescent primer. The DNA fragments were analyzed using a CEQ 8000 DNA Fragment Analyzer. Results27 to 46, 5 to 20 and 4 to 31 pieces of amplified products were detected from Papaver somniferum L, Cannabis sativa L and Papaver rhoeas L respectively, and significant difference was observed among these three groups of products. The identical species-specific peaks were identified from root, stem, leaf, flower and seed DNA from the same Papaver somniferum L. ConclusionThe diacritical results from Papaver somniferum L, Papaver rhoeas L and Cannabis sativa L, as well as the identical results of different parts from the same plant demonstrated that AFLP analysis could be used possibly to determine the species origin of unknown plants samples.

Objective To discuss the value of mammotome biopsy of breast cakified lesions under the real time guidance of mammography.Methods The data of 42 patients with 44 mammary gland calcification(2 patients with bilateral lesions)who underwent mammotome biopsy under the real time guidance of a mammography from Jan.2011 to Feb.2012 in the First Affiliated Hospital of Chongqing Medical University were retrospective analyzed.Rapid paraffin pathology was conducted.Results Among the 44 lesions,34 were benign(27 hyperplasia,2 fibroadenoma,1 lipoma,1 cyst,3 others)and 10 were breast cancer(a benign lesion diagnosed as breast cancer by postoperative biopsy was included).Among the 42 patients,32 patients had benign lesions,accounting for 76.2％ (32/42),10 patients had breast cancer,accounting for 23.8％ (10/42).All lesions were completely resected during the operation.Malignant lesions were given treatment such as modified radical mastectomy,breast conservation surgergery,chemotherapy and so on.A single lesion resection took about 20 minutes.During the follow-up of 3 months,no significant event happened.Conclusions Through the qualitative diagnosis of mammotome biopsy under the real time guidance of mammography,we can get early diagnosis of breast cancer.Early diagnosis and early treatment can improve the life quality and survival time.It is more important to detect early breast cancer which was considered benign lesions clinically.Mammotome biopsy under the real time guidance of mammography has several advantages including easy to operate,accurate positioning and less complications,making it the preferred approach in managing mammary gland calcification especially suspicious malignant calcification.

Objective To study the induction of IL-6 on expression and activity of tissue factor (TF)in peripheral blood monocytes(PBMCs).Methods rhIL-6 100 ng/L and rhIL-6 100 ng/L+ rhIL-6 MoAb 10?g/L were used respectively to stimulate the PBMCs for 24 h,PBMCs without any stimulation as control group,to study the changes of antigen content,mRNA expression and the ac- tivity of the TF.Results The antigen content,mRNA expression and activity of TF were increased significantly in 100 ng/L rhIL-6 group as compared with rhIL-6 100 ng/L+rhIL-6 MoAb 10?g/L and control groups(P＜0.01).Conclusions rhIL-6 can induce the increase of antigen expression,activity and mRNA expression in PBMCs,and these effects can be suppressed successfully by rhIL-6 MoAb. This study demonstrated that there was potential relations between cytokines and thrombogenesis, which may play an important role the pathogenesis of chronic rejection.

Echinacoside has various pharmacological effects, such as antioxidative, antisenescence, neuroprotection, antiinflammation, promotion of cicatrization, hepatoprotection, promotion of bone formation, and antitumor activity. There are some progress in its pharmacokinetics study. Echinacea has therapeutic effect on diseases in various systems. It has great significance to further research and develop echinacoside.

AIM:To perform a new method for orbital implant and contracted socket through one time and its results. ·METHODS:Totally 114 patients 114 eyes, from January 2008 to June 2014, with contracted socket participated in this study. We incised the bulbar conjunctiva horizontally and excised scar tissue, then implanted the hydroxyapatite in the four extraocular muscles and tightly sutured the Tenon ' capsule. After that, we put the superior and inferior conjunctival petals backwards and sutured them to the Tenon ' s capsule. All the patients were divided into four groups according to the vertical diameter length of the conjunctival defect area:GroupⅠ:≤5mm; Group Ⅱ: 6-10mm; Group Ⅲ: 11-15mm; and Group Ⅳ: ≥16mm. These patients were followed up for 6mo to 3y to observe the conjunctival sac shaping and growth of conjunctiva. ·RESULTS:There were 64 cases in GroupⅠ, 31 cases in Group Ⅱ, 16 cases in Group Ⅲ and 3 cases in Group Ⅳ. All patients ' conjunctival defect was covered by new conjunctiva and scar tissue 4 to 6wk after surgeries. Ten cases had contracted socket; 2 cases had orbital implant exposure, requiring reoperation. Of the 114 cases, 8 had contracted socket and could use a smaller conformer, 106 could use a normal size conformer. ·CONCLUSION: When the conjunctival defect was ≤15mm, this new method can address the orbital implant and contracted socket at the same time. While it was ≥16mm, flap transplantation is necessary.