Objective To explore the signification and method of Cut-off verification and gray zone setting in chemiluminescent assay.Methods NCCLS EP-12 A2 document defines that C50 is the analyte concentration of cut off value for immunology qualitative test and C5-C95 interval is the range of analyte concentration that yields 5％ positive results to 95％ positive results for immunology qualitativc test.The C50 and C5-C95 interval of HBeAg in ARCHITECT i2000 were worked out according to the cut off value provided by HBeAg reagent calibrated in ARCHITECT i2000,which were verified to approve the character declaimed by manufactory or not.Gray zone was set and the procedure of cut off verification and gray zone set in chemiluminescent were built; A set of quality control was detected 20 times with two different lot HBeAg reagent kits,S/CO was caculated and compared with t test.Results C50 and C5-C95 interval of reagent (lot 06087L100,96378HN00) were 0.171 PEI U/ml,0.125 PEI U/ml; ＞0.154 PEI U/ml to 0.188 PEI U/ml,0.119 PEI U/ml to ＜0.150 PEI U/ml,respectively.S/CO of negative quality control and positive quality control were (0.550 ±0.038),(2.422 ±0.084) and(0.334 ±0.063),(3.587 ±0.321),respectively.They all approved the character (the sensitivity at cut off was less than 0.5 PEI U/ml)declaimed by manufactory,and the results of S/CO between two lot kits were obvious difference (t =9.944,15.499,P ＜0.01).Conclusion C50 and C5-95 interval can be used to verify cut off value and set gray zone in chemiluminescent assay;They may vary in different lot reagents and they must be verified to approve the character declaimed by manufactory.

Objective To evaluate the diagnostic accuracy of imprint cytology for intracranial tumors during operation. Methods 150 cases of intracranial tumors were diagnosed by imprint cytology. The imprint was prepared by touching the specimens several times with clean slide, and then the slides were fixed and stained with HE. The diagnostic results obtained by imprint cytology were compared with those made by frozen and routine paraffin sections. Results The accurant rates of diagnosing for non-glial tumors, classifying for incracranial tumors and grading for glial tumors were 100%, 91.3% and 88.3%, respectively. In addition, the morphological features of glial tumors, meningioma, pituitary adenoma on imprint cytology were briefly described. Conclusion The accurant rate of imprint cytology is almost equal to that of frozen section, and both the detection methods have a supplementary effect to each other.

Objective In order to understand the mechanism of solute transperitoneal transport, we studied the relationship between IL-8 level and protein transperitoneal transport. Method 12 New Zealand White rabbits were randomly divided into experimental and control groups. 2 5% glucose peritoneal dialysis solution (40ml/kg)contained 3?10 9 CFU staphylococcus aureus 1ml was injected into the abdominal cavity of experimental NZW rabbits and 2 5% glucose peritoneal dialysis solution (40ml/kg)contained 0.9%NaCl solution 1ml was injected into the abdominal cavity of controlling NZW rabbits. The plasma and effluent concentrations of blood creatinine ,glucose,total proteins and albumine were determined respectively, the D/P or D/Do values of creatinine , total proteins ,albumine or glucose were calculated respectively. Results The D/P ratios of creatinine, total proteins and albumin were increased significantly, while the D/Do of glucose was decreased in experimental group on different dwelling time points ,there was a significantly difference,as compared with the control group (P0 05),while there was significantly correlation after 60 mins(P

Adult ; Humans ; Hydrochloric Acid ; poisoning ; Male ; Middle Aged ; Smoke Inhalation Injury

Diagnosis, Differential ; Drugs, Chinese Herbal ; therapeutic use ; Extracellular Matrix Proteins ; biosynthesis ; Humans ; Liver ; metabolism ; pathology ; Liver Cirrhosis ; drug therapy ; metabolism ; pathology ; Medicine, Chinese Traditional ; Phytotherapy

Objective To understand the current status and the changes in main disability reasons in the elderly with physical disabilities during the past 20 years in China. Methods　The data of the elderly with physical disabilities aged 60 years and over that were from the national sample survey on disability in 1987 and 2006 were analyzed and compared by epidemiology methods. Results　In 2006.the main disability reasons in the elderly with physical disabilities aged 60 and over were disease factors,injury factors,other factors and congenital diseases and eccyliosis.The cause-specific disability prevalences were 5.262%,1.885%,1.000% and 0.200%respectively.Cerebrovaseular diseases showed the highest cause-specific disability prevalence of 2.552% in all the causes.Compared with the national sample survey in 1987,the cause-specific disability prevalences of disease factors and injury factors were obviously increased.The top five disability reasons in the elderly with physical disabilities aged 60 years and over in China were cerebrovascular diseases,arthropathy,miscellaneous traumas,other causes and unknown causes in the national sample survey in 2006;while vascular diseases,other traumas,other causes,unknown causes and pyogenic infection were the top five disability reasons in the national sample survey in 1987.The age-specific top five reasons were basically the same with the total top five disability reasons of the elderly with physical disabilities aged 60 years and over,and also the same relationship between sex-specific top five reasons and Iotal top five disability reasons in 1987 and 2006. Conclusions　Disease factors are the main disability reasons of the elderly with physical disabilities aged 60 years and over,and cerebrovascular diseases have the highest cause specific disability prevalence.The prevention and cure of cerebrovascular diseases,arthropathy and other traumas become the key points to prevent physical disability in the elderly.

Bronchoscopes ; Bronchoscopy ; instrumentation ; methods ; Child ; China ; Humans

Abdominal drainage is the most common technique applied in the abdominal surgery. According to the aim of drainage, it can be divided into curative drainage and preventive drainage, but there is no obvious difference between the 2 drainages. Abdominal drainage is not necessary after parenchymal viscera operation, but necessary after spleenectomy in preventing infection. For cavity viscera operation, abdominal drainage is applied according to the infectious condition, but scholars at home and abroad have different opinions on this point. Surgeons should pay attention to the placement of the drainage tube in patients who received preventive drainage.

To retrospectively analyze the clinical data of 305 cases with apoplectic sequela admitted at our general geriatric nursing ward from January 2000 to December 2011.The subjects were divided into 2 groups:hypoalbuminemia and non-hypoalbuminemia.Advanced age (OR =5.624),chronic heart failure (OR =2.298),conscious disturbance (OR =1.575),dysphagia (OR =1.565),complete bedridden (OR=2.874),pneumonia (OR =3.725) and bedsore (OR =5.336) were risk factors for hypoalbuminemia in the patients with apoplectic sequela.And hyperglycemia(OR =0.066)was a protective factor for it.

Colorectal polyps are common digestive system diseases in children. The important role of endoscopy in the di-agnosis and treatment of colorectal polyps in children has been recognized. With the continuous development of endoscopic techniques, the diagnosis and treatment of colorectal polyp has become safer, more convenient and more effective. In this re-view, the progress in endoscopy for diagnosis and treatment of colorectal polyps in children has been summarized.