Objective To explore the signification and method of Cut-off verification and gray zone setting in chemiluminescent assay.Methods NCCLS EP-12 A2 document defines that C50 is the analyte concentration of cut off value for immunology qualitative test and C5-C95 interval is the range of analyte concentration that yields 5％ positive results to 95％ positive results for immunology qualitativc test.The C50 and C5-C95 interval of HBeAg in ARCHITECT i2000 were worked out according to the cut off value provided by HBeAg reagent calibrated in ARCHITECT i2000,which were verified to approve the character declaimed by manufactory or not.Gray zone was set and the procedure of cut off verification and gray zone set in chemiluminescent were built; A set of quality control was detected 20 times with two different lot HBeAg reagent kits,S/CO was caculated and compared with t test.Results C50 and C5-C95 interval of reagent (lot 06087L100,96378HN00) were 0.171 PEI U/ml,0.125 PEI U/ml; ＞0.154 PEI U/ml to 0.188 PEI U/ml,0.119 PEI U/ml to ＜0.150 PEI U/ml,respectively.S/CO of negative quality control and positive quality control were (0.550 ±0.038),(2.422 ±0.084) and(0.334 ±0.063),(3.587 ±0.321),respectively.They all approved the character (the sensitivity at cut off was less than 0.5 PEI U/ml)declaimed by manufactory,and the results of S/CO between two lot kits were obvious difference (t =9.944,15.499,P ＜0.01).Conclusion C50 and C5-95 interval can be used to verify cut off value and set gray zone in chemiluminescent assay;They may vary in different lot reagents and they must be verified to approve the character declaimed by manufactory.

Objective In order to understand the mechanism of solute transperitoneal transport, we studied the relationship between IL-8 level and protein transperitoneal transport. Method 12 New Zealand White rabbits were randomly divided into experimental and control groups. 2 5% glucose peritoneal dialysis solution (40ml/kg)contained 3?10 9 CFU staphylococcus aureus 1ml was injected into the abdominal cavity of experimental NZW rabbits and 2 5% glucose peritoneal dialysis solution (40ml/kg)contained 0.9%NaCl solution 1ml was injected into the abdominal cavity of controlling NZW rabbits. The plasma and effluent concentrations of blood creatinine ,glucose,total proteins and albumine were determined respectively, the D/P or D/Do values of creatinine , total proteins ,albumine or glucose were calculated respectively. Results The D/P ratios of creatinine, total proteins and albumin were increased significantly, while the D/Do of glucose was decreased in experimental group on different dwelling time points ,there was a significantly difference,as compared with the control group (P0 05),while there was significantly correlation after 60 mins(P

Objective To evaluate the diagnostic accuracy of imprint cytology for intracranial tumors during operation. Methods 150 cases of intracranial tumors were diagnosed by imprint cytology. The imprint was prepared by touching the specimens several times with clean slide, and then the slides were fixed and stained with HE. The diagnostic results obtained by imprint cytology were compared with those made by frozen and routine paraffin sections. Results The accurant rates of diagnosing for non-glial tumors, classifying for incracranial tumors and grading for glial tumors were 100%, 91.3% and 88.3%, respectively. In addition, the morphological features of glial tumors, meningioma, pituitary adenoma on imprint cytology were briefly described. Conclusion The accurant rate of imprint cytology is almost equal to that of frozen section, and both the detection methods have a supplementary effect to each other.

The configuration of the air cleaner and operating principle of high-voltage static electrical field (HVEF) are introduced. The primary bactericidal mechanism and broad-spectrum effect are also mentioned.

Objective:To evaluate the application status and change trends of insulin and its analogs in Nanjing area during 2011 to 2013. Methods:The varieties, sales amount and DDDs of insulin and its analogs in 43 hospitals of Nanjing during 2011 to 2013 were analyzed. Results:The use of insulin and its analogs was increased year by year from 2011 to 2013 with the average annual growth rate of 16%. Glargine, pre-mixed insulin 30R, aspart 30, human insulin and aspart ranked the top five in the sum of money with the proportion above 80%. Pre-mixed insulin 30R, aspart 30, glargine, human insulin and common insulin ranked the top 5 in DDDs. Conclusion:The application of insulin and its analogs is wide in clinics, and the usage and sum of money both show rapid growth trend.

Autonomic nervous system(ANS) activity plays an important role in the pathogenesis of cardiovascular disease,including organic cardiovascular disease(such as hypertension,coronary artery disease,etc) and functional cardiovascular disease (such as vasovagal syncope,postural orthostatic tachycardia syndrome,etc).Many methods was used to evaluate ANS function.Heart rate variability is a widely accepted method to assess cardiac autonomic nerve function.Autonomic nervous affected cardiac action potential and let electrocardiogram changes.Through the sympathetic and parasympathetic neurotransmitters which acting on the corresponding receptors.Electrocardiogram changes reflects autonomic nervous function.Present researches suggest that electrocardiogram has an important significance in the evaluation of autonomic nerve function in functional cardiovascular disease.

0.05).Conclusion:HLA-DQA1*0102 and DQA1*0104 were associated with protection from chronic HBV infection and development of LC,respectively.There was no significant association between HLA-DQA1 and development of HCC in chronic HBV infection patients.

Objective:To explore the prevalence of posttraumatic stress symptoms among preschool children and its related factors after the Ludian earthquake.Methods:A total of 62 children's guardians were visited.They were interviewed with the Posttraumatic Stress Disorder Semi-Structured Interview for Infants and the Young Children and the Child Behavior Checklist-Posttraumatic Stress Disorder scale (CBCL-PTSD) consisted of 15 items from the CBCL.All of the participantsresided in Longtoushan settlement and Longtoushan town (the epicenter).They were randomly visited from west to east in that region.Results:The rate of scored 9 (or more) on the CBCL-PTSD among children was 51.9％ (27/52) in 1-2 months after the earthquake.However,based on the Semi-Structured Interview,the rate of the children's posttraumatic stress symptoms was 26.9 ％ (14/52).The numbers of posttraumatic stress symptoms,intrusion symptoms,avoidance symptoms,hyperarousal symptoms,the new symptoms and the CBCL-PTSD scores were positively correlated (0.30-0.73,P ＜0.05),except the relation between intrusion symptoms and hyperarousal symptoms.The rate of intrusion symptoms was significant higher among those who experienced earthquake than those who didn't (96.2％ vs.60.0％,P ＜0.01).And the rate of intrusion symptoms was also higher among those who was injured during the earthquake than those who wasn't (100％ vs.84.6％,P ＜ 0.05).And the other symptoms were not difference in children under different conditions (P ＞ 0.005).Conclusion:The preschool children who experienced earthquake or were injured suffer more posttraumatic stress symptoms.

Objective To analyze the characteristics and diagnosis of children aged

Bronchoscopes ; Bronchoscopy ; instrumentation ; methods ; Child ; China ; Humans