Highly active antiretroviral therapy (HAART) can effectively suppress thehuman immunodeficiency virus (HIV) replication andsignificantly reduce morbidity and mortality of HIV-infectedpatients,which howevercan't completely remove the virus,and eventually progressinto chronic viral-infection disease.Chronic HIVinfection destroys host immune system,leading to intestinal barrier damage,intestinal mucosal dysfunction,microbial translocation,and further accelerates the disease progress.The reconstruction of intestinalmicroflora balance and improvement of intestinal mucosa function areessential to reestablish the host immune system.This paper will review the current research advanceson intestinal barrier damage of HIV infection and gut-target therapy of AIDS.The aim is to provide valid evidences for further research targeting improvement of treatment strategiesandreduction of morbidity and mortality in HIV infection.

Objective To transduct with human telomerase reverse transcriptase(hTERT) to Schwann cells via electransfection technique to prolong the life span and enhance the proliferative capacity of Schwann cells.Methods The hTERT RNA was derived from esophagus cancer tissue and pcDNA3.1-hTERT vector was built.With electransfection technique, we tmnsfected vector into ADSCs.The PCR,TRAP-PCR, and PI-annexin V were tested to prove the expression of hTERT in ADSCs.Results After transduction the hTERT RNA in Schwann ceils, TRAP-PCR test, PI-annexin V dying and S100 dying was positive.Conclusion Telomerase catalytic subunit(hTERT) is transduct into Schwann cells, and it can to enhance the prolifertative capacity.

PurposeTo study the pharmacokinetic characteristics of omeprazole in Chinese extensive and poor metabolizers.Methods The pharmacokinetics of omeprazole was studied in eighteen healthy volunteers. After a single oral dose of omeprazole capsule(40 mg), the plasma concentrations of omeprazole and its two metabolites, 5-hydroxyomeprazole and omeprazole sulfone, were determined with reversed HPLC method. The plasma concentration-time data were analyzed to estimate the pharmacokinetic parameters. Results Both plasma omeprazole concentration and the pharmacokinetic parameters exhibited marked interindividual variation. The metabolic ratio (MR= plasma omeprazole/5-hydroxyomeprazole) obtained 3.5 h after medication was used to distinguish between extensive and pcr)r metabolizers (EMs, PMs). The variances of AUC(0-12) caused by the two metabolizer phenotypes accounted for 75.4 % of the total interindividual variances. AUC(0-12) of omeprazole was (1 971.78 ±1 221.78)ng·h/ml in EMs( n=12) and (8 587.18±2 855.48) ng·h/ml in PMs (n = 6),respectively (P<0.01),and CL in EMs and PMs was estimated as (16.00±9.71) and (4.79±1.32) L/h (P<0.01). Accordingly,significantly lower level of plasma 5-hyclroxyomeprazole was found in PMs, revealing a slower hydroxylation rate compared with EMs. Conclusions The results suggest that individualized dose regimen of omeprazole, based on identification of metabolizer phenotype, can be of great benefit from the viewpoint of pharmacoeconomics.

To investigate the clinical efficacy of massage manipulation reposition for treatment of child hip-joint semiluxation, sixty-seven cases of child hip-joint semiluxation were treated by massage manipulation reposition. Sixty-two cases were cured and 5 cases improved. The total effective rate was 100%. Massage manipulation reposition is an effective method for treating child hip-joint semiluxation.

Objective To assess the value of high-resolution ultrasonography in diagnosis of cubital tunnel syndrome. Methods Forty-two patients (43 elbows) initially diagnosed as cubital tunnel syndrome underwent ultrasonography (US), while 15 healthy contralateral elbows of these patients taken as self controls, and 15 healthy volunteers as normal controls underwent US of the ulnar nerve. The findings of US measurements were compared with that of intra-operative results and pre-operative electromyography. Results High-resolution US displayed changes and some etiological factors of cubital tunnel syndrome. The measurements of ulnar nerve at the proximal part of the compression were higher than those in the control groups. The cut-off point of cross-sectional area (CSA) and CSA tumefaction ratio was 0.11 cm~2 and 141.50%, respectively. The sensitivity of US was 92.86% compared with intra-operative results, and was 100% when combined with pre-operative electromyography. Conclusion High-frequency ultrasound can be used as an effective method to diagnose cubital tunnel syndrome.

Objective To study the recent effect of ovary dislocation during the operation of cervical squa- mous carcinoma with stageⅠon ovary function.Methods 12 cases with cervical squamous carcinoma of stageⅠwere treated with surgery.During the operation bilateral ovaries with blood vessels were dislocated the abdominal cavity over the umbilical line through postperitoneum and fixed on the postperitoneum.12 cases were hysterec- tomized simply as the control.The change of ovary function was observed in one year after the operation.Results The levels of FSH and E_2 were decreased in the dislocating group(P

Objective To compare the analgesic efficacy and side effects of patient-controlled intravenous analgesia (PCIA) with Lornoxicam and patient-controlled epidural analgesia(PCEA) with morphine in patients undergoing Orthopaedics surgery. Methods 100 ASA Ⅰ-Ⅱ Patients scheduled for Orthopaedic surgery were divided randomly into two groups: Lornoxicam PCIA group( n =50) , Lornoxicam 48mg was diluted to 100ml with normal saline (0.9%NS). Morphine PCEA group( n =50), Morphine 9mg + bupivacaine 150mg were diluted to 100ml with normal saline (0.9%NS) .In both groups the patients were received PCA at a rate of 2ml/h with the bolus dose of 0.5ml and lockout interval of 15min. The loading dose was Lornoxicam 8mg plus Ondansetron 4mg in group PCIA and morphine 1mg and Ondansetron 4mg diluted to 10ml with normal saline (0.9%NS) in group PCEA 30 minutes before the end of operation. BP, RR, ECG, SpO_2 were monitored continuously after operation. Efficacy of analgesia was assessed by VAS scores. Side effects such as drowsiness, sweat, nausea, vomiting and skin pruritus were also observed. Results There was no significant difference in the mean VAS score between group PCIA and group PCEA at 4h, 8h, 16h ,20h, 24h, 32h and 48h. after operation. There was no significant difference in side effects between the two groups except the occurrence of sweat, nausea, vomiting and skin pruritus which was lower in group PCIA than in group PCEA ( P

With growing dependence on picture archiving and communication systems(PACS) for viewing images,a quality assurance program to the condition of workstation displays and image quality guidelines for better cost-efficacy and diagnostic accuracy has become increasingly important.This article analyses and synthesizes the quality control of the PACS phantom workstation mainly from such aspects as the monitor resolution and brightness,the environment degree of illumination,statistics assessment method(ROC curve) and so on.

Objective To observe the effect on body distribution tendency of paclitaxel liposome in the lung cancer rats after acupuncture of Feishu,Taiyuan acupoints in order to develop the new path for the target study of anticancer drugs.Method The paclitaxel liposome was labeled by technetium(Tc).Four methods including tail intravenous injection of paclitaxel liposome merely and the combination of tail intravenous injection of paclitaxel liposome with acupuncture of Feishu,Taiyuan(concerned acupoint) and Shenmen(unconcerned acupoint) acupoints respectively were carried on the intervention to the lung cancer rat models(18 in each group).The rats were sentenced to death at 60,90,120 minutes(6 at each time point) respectively after drug injection,and the drug content of paclitaxel liposome in the lung,liver,kidney organs were measureed by the ?-radiate immunity counting implement respectively.Result Acupuncturing at Feishu and Taiyuan can lead to the drug content increment of paclitaxel liposome in the lung organization of the lung cancer rats in all or parts of time points.On the contrary,acupuncturing at Shenmen can not.Conclusion Acupuncturing Feishu and Taiyuan can make tropism effect on the disposition of paclitaxel liposome in vivo in lung cancer rats.Feishu is stronger than Taiyuan on the drug tropism of the lung.

With the enforcement of YY 0505-2012 standard,more attention has been paid to the electromagnetic compatibility test of medical electrical equipment.In all the test items involved in YY 0505-2012,the radiation emission test is the most complicated and the hardest to be passed,so it is very important to master the test method.Microwave therapy equipment is a kind of equipment using microwave radiation energy to treat diseases,and is supervised as class Ⅲ medical equipment.According to the requirements of the relevant standards,all the electromagnetic compatibility testing items of microwave equipment are listed,and the test difficulty and the test focus of the equipment are studied.In the frequency band of the radiation emission experiment,microwave treatment equipment is divided into different categories according to the purpose and environment of the application.By comparing the data of the tests,the differences of different classes of microwave treatment equipment in the radiation emission tests are summarized.