1.Application of needle aspiration cytopathology in the lymphoma diagnosis
Dongmei ZHOU ; Gang CHEN ; Cheng HE ; Xiongwei ZHENG ; Chao LI
Journal of Leukemia & Lymphoma 2013;22(11):674-677
Objective To investigate the feasibility of fine needle aspiration cytopathology in the diagosis of lymphoma.Methods To compare cytopathology with histopathology of 72 lymphoma cases and analyse immunochemistry stain of 3 cases.Results 61 of 72 cases were diagnosised as lymphoma in cytopathology,while 58 of 61 cases were diagnosised as lymphoma in histopathology.The sensitivity,specificity,accuracy,positive predictive value and negative predictive value rates of cytopathology were 89.2 % (58/65),57.1% (4/7),86.1% (62/72),95.1% (58/61),and 36.4 % (4/11) respectively.Conclusion Fine needle aspiration cytopathology is significant to the diagnosis of lymphoma.It should greatly improve the accuracy of lymphoma diagnosis and make it possible to subclassify lymphoma,combined cytopathology with other auxilary detection.
2.Effect of Baoxin capsule on hemodynamics of myocardial ischemia in dogs
Hui YANG ; Weikang WU ; Jianping CHEN ; Lin ZHOU ; Chao CHENG
Chinese Journal of Pathophysiology 1986;0(03):-
AIM: To observe the effect of Baoxin capsules on hemodynamics of ischemic myocardium of dogs. METHODS: Fed Baoxin capsule for continuous three days, left anterior descending branch of the coronary artery of the dogs were ligated to observe the change of cardiac output, blood pressure and ?dp/dt max in different time of myocardial ischemia. RESULTS: Baoxin capsules increased the blood flow obviously, and increased left ventricular function simultaneously, the difference with ischemic group is significant (P
3.The impact of cigarette cessation intervention on mental state of patients with coronary heart disease
Zhiming ZHOU ; Xiaohan XU ; Jing LIANG ; Bin HU ; Yujing CHENG ; Chao SHI ; Yujie ZHOU
Chinese Journal of Internal Medicine 2016;55(11):854-858
Objective This study was designed to observe the impact of cigarette cessation on anxiety and depression in patients with coronary heart disease (CHD).Methods A total of 690 cigarette smoking patients with CHD identified by coronary angiography (CAG) were included and analyzed in the study.The mental state were scored with Hamilton anxiety (HAMA) and depression (HAMD) scale both on admission and at 6-month follow-up.The patients were divided into two groups based on the cigarette cessation.The score of mental state between the two groups were compared.The patients were treated with percutaneous coronary intervention (PCI),coronary artery bypass grafting (CABG),or medicine therapy (MT).Results The clinic data and score of mental state were similar at the time of admission (HAMA:10.66±5.53 vs 11.09 ±5.61,P =0.311;HAMD:29.81 ± 10.13 vs 28.94 ± 10.22,P =0.266 4) between the two groups.After 6 months,the proportions of subjects in smoking cession group with anxiety (24.2% vs 32.3%,P <0.05),depression (18.0% vs 27.5%,P <0.05),and anxiety and depression (7.0% vs 16.2%,P <0.001) decreased significantly compared with those in smoking group irrespective of the treatment strategy.Both the HAMA and HAMD scores were lower in smoking cessation group (HAMA:9.83±3.40;HAMD:24.91 ±7.90) than in smoking group (HAMA:10.98 ±4.87;HAMD:27.70 ± 11.16) (all P < 0.001).Conclusions Smoking cessation is good for the relief of anxiety and depression in CHD patient.
4.Culture conditions optimization and high cell density fermentation of recombinant bacteria producing heparinase II from Flavobacterium heparinum.
Bin ZHOU ; Yongmei CHENG ; Chao DENG ; Weichao LIU ; Chaoliang CHEN ; Jinghua CHEN ; Zhenghong XU
Chinese Journal of Biotechnology 2014;30(4):674-678
Heparinase II (Hep II) from Flavobacterium heparinum is an enzyme that could specifically cleave certain sequence of heparin and heparan sulfate. In this work, fermentation conditions of recombinant heparinase II (His-Hep II) producing bacteria were optimized, including initial induction time, inducer (IPTG) concentration, induction temperature and induction time. The optimum conditions were as follows: cultivating recombinant bacteria to exponential prophase under 37 degrees C, then adding IPTG to a final concentration of 0.3 g/L, finally cultivating recombinant bacteria under 20 degrees C for 10 h. The total crude enzyme activity reached 570 U/L. Based on these results, high cell density fermentation of recombinant bacteria was studied. The final OD600 could reach 98 and the total crude enzyme activity of His-Hep II increased to 9 436 U/L.
Fermentation
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Flavobacterium
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metabolism
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Microbiological Techniques
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Polysaccharide-Lyases
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biosynthesis
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Recombinant Proteins
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biosynthesis
5.Identification of Epmedii Folium and its counterfeit leaf of Quercus variabilis.
Shuai KANG ; Chao ZHOU ; Yi HE ; Ji ZHANG ; Ai-hua WEI ; Jing LU ; Shuang-cheng MA
China Journal of Chinese Materia Medica 2015;40(9):1676-1680
Epmedii Folium is a commonly used traditional Chinese drug, and is beneficial for the "liver" and "kidney" s function in Chinese medicine. Recently, the origin of this drug is more complex. Most of the identification studies are emphasized on the species certified by the pharmacopoeia and other related species from the same genus of Epimedium, but few was emphasized on the counterfeit. In this paper, one counterfeit of Epmedii Folium, identified as the dried leaf of Quercus variabilis (Fam. Fagaceae), has been reported based on field investigation, comparing specimen of Epmedii Folium and Q. variabilis,using the macroscopic, microscopic and TmC methods. It is resulted that they could be identified clearly not only by the macroscopic features, such as the vein character and the tooth apex, but also by the microscopic features, such as the vascular bundles of the midrib, the non-glandular hair, the anticlinal wall of the epidermis cell and the calcium oxalate crystal. Furthermore their TLC chromatograms showed also difference. This study will give reference for the identification of Epmedii Folium and the related supervision and inspection work.
China
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Discriminant Analysis
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Epimedium
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anatomy & histology
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chemistry
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Plant Leaves
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anatomy & histology
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chemistry
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Plants, Medicinal
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anatomy & histology
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chemistry
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Quercus
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anatomy & histology
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chemistry
6.Correlation between thyroid function and glucolipid metabolism in type 1 diabetic adults
Yiping CHENG ; Xinli ZHOU ; Fei JING ; Lei KONG ; Ling GAO ; Qingbo GUAN ; Jiajun ZHAO ; Chao XU
Chinese Journal of Internal Medicine 2021;60(1):51-54
To assess the correlation between thyroid function and glucolipid metabolism in type 1 diabetic adults. A retrospective analysis was conducted in 230 type 1 diabetic adults who were hospitalized in the Department of Endocrinology of Shandong Provincial Hospital Affiliated to Shandong University from January 2008 to January 2020. It showed that thyroid stimulating hormone(TSH) was significantly positively correlated with total cholesterol (TC) ( r=0.239), triglycerides (TG) ( r=0.166) and low-density lipoprotein cholesterol (LDL-C) ( r=0.249), respectively (all P<0.05). Free triiodothyronine (FT 3) was significantly negatively correlated with fasting plasma glucose (FPG) ( r=-0.272), glycated hemoglobin (HbA1c) ( r=-0.240), TC ( r=-0.197) and LDL-C ( r=-0.220), respectively (all P<0.05). Free thyroxine (FT 4) was negatively correlated with TC ( r=-0.171) and LDL-C ( r=-0.170), respectively (all P<0.05). TC was an independent predictor of TSH, FT 3 and FT 4, FT 3 and FT 4 were independent predictors of HbA1c. TSH was an independent predictor of TC, TG and LDL-C. Thyroid function is closely related to glucolipid metabolism in type 1 diabetic adults.
7.Therapeutic effectiveness of low-power Nd:YAG laser on myofascial pain syndrome:73 cases report
Yan WANG ; Tong-chao GENG ; Bao-yu ZHOU ; Liru CHENG ; Heyu KANG
Chinese Journal of Rehabilitation Theory and Practice 2002;8(9):550-550
ObjectiveTo investigate the therapeutic effectiveness of low-power laser on myofascial pain syndrome. Methods73 self-controlled patients with myofascial pain syndrome were irradiated on myofascial trigger points with Nd:YAG laser in wavelength 830nm, power 500mW, 20 minutes per day for 5 times. At pre-and post-treatment,pain intensity and pressure pain of myofascial trigger points were checked. ResultsAfter treatment, score of pain intensity was reduced signficantly from (7.24±2.41) to (2.21±1.22) (P<0.001). The pressure pain of myofascial trigger points were improved . Conclusions Low-power laser can reduce the pain intensity and increase the pressure pain threshold of myoficial trigger points.
8.Research advances in neonatal nasal intermittent positive pressure ventilation.
Zhi-Cheng ZHU ; Jian-Guo ZHOU ; Chao CHEN
Chinese Journal of Contemporary Pediatrics 2017;19(12):1301-1305
Nasal intermittent positive pressure ventilation (NIPPV) can augment nasal continuous positive airway pressure (nCPAP) by delivering intermittent positive pressure ventilation in a noninvasive way and can provide a new option for neonatal noninvasive respiratory support. NIPPV has an advantage over nCPAP in primary and post-extubation respiratory support. Moreover, it can reduce severe apnea of prematurity. Synchronized NIPPV has promising application prospects. This review article summarizes the advances in the application of NIPPV in neonatal respiratory support to promote the understanding and standardization of this technique.
Airway Extubation
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Humans
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Infant, Newborn
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Intermittent Positive-Pressure Ventilation
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adverse effects
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instrumentation
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methods
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Prognosis
10.Screening of TNF-alpha antagonist peptides from a random peptide library displayed with Escherichia coli flagellar.
Chao LI ; Yan-Rong ZHOU ; Du-Sheng CHENG ; Pei-Tang HUANG
Chinese Journal of Biotechnology 2002;18(5):556-560
Tumor necrosis factor(TNF-alpha) plays an improtant role in the process of anti-infection and anti-cancer. It can both protect and make damage to the host. In order to find new way of inhibiting its host-damaging activity, An E. coli flagella displayed random peptide library was constructed and TNF-alpha antagonist peptides were screened using the peptide library. After 5 rounds of panning and DNA sequencing, six peptide sequences were obtained. Two of them(TBP2, TBP3) have the same sequence frame of V--N-WG. After primary comparation of TNF-alpha binding ability, four peptides were synthesised and purified with RP-HPLC. The activity of inhibiting TNF-alpha was detected with L929 cell and MTT method. The data show that TBP2 and TBP3 can inhibit 90% of TNF-alpha activity when TNF-alpha gives about 30% cell toxicity on L929. The two sequences have not been reported.
Escherichia coli
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genetics
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Peptide Library
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Peptides
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isolation & purification
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pharmacology
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Tumor Necrosis Factor-alpha
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antagonists & inhibitors