Adult ; Antibodies, Monoclonal, Murine-Derived ; administration & dosage ; therapeutic use ; Female ; Hemophilia A ; drug therapy ; Humans ; Rituximab

OBJECTIVE: To develop an HPLC method for the determination of Gastrodin in Qianglitianmaduzhong capsule. METHODS: The samples were separated on Diamond C18 column (250 mm?4.6 mm,5?m).The mobile phase consisted of acetonitrile - 0.1% phosphoric acid(2∶98) at a flow rate of 1.0 mL?min-1.The UV detection wave length was set at 210 nm and the column was kept at room temperature. RESULTS: The linear range of Gastrodin was 0.126 4～3.160 0 ?g(r=0.999 8), and the average recovery was 98.30%(RSD=0.8%,n=6).CONCLUSION: The method is suitable for the determination of gastrodin in Qianglitianmaduzhong capsule.

Objective To compare the effects of desflurane and propofol on systemic hemodynamics and arterial oxygenation undergoing one-lung ventilation (OLV) during video-assisted thoracoscopic surgery (VATS)Methods Twenty patients,ASA physical status Ⅱ or Ⅲ, scheduled for elective VATS undergoing OLV , were prospectively randomized into two groups : desflurane group(group D,n=10) and propofol group (group P,n=10) Anesthesia was induced with intravenous propofol 2mg?kg -1(group P) or thiopental 5 mg?kg -1( group D) combined with intravenous fentanyl 4?g?kg -1 ,vecuronium 01mg?kg -1 and succinylcholine 100mg , and was maintained with infusion of propofol at rate of 2-8 mg? kg -1?h -1 (group P) or inhalation of desflurane 2%-4% (group D) The Carlen double-lumen endobronchial tube was intubated to ventilated mechanically with 100% oxygen MAP and HR were monitored during the entire procedures, and blood gas analysis was made before operation, 30 min following two-lung ventilation, 30 min and 60 min following OLV in both groups,respectively Results PaO 2 were decreased significantly following OLV in both groups(P005)Conclusions Desflurane can be used as safely as propofol with one-lung ventilation during video-assisted thoracoscopic surgery

The explosive terror attack is the most frequent in all terror activities in the world. The authors reviewed its current situation and the challenges we are confronted with in China and elsewhere. In the present paper, the types, characteristics and emergency medical support (particularly classifying and emergency treatment, triage, transportation and evacuation, seeking and unearthing for buried victims) of explosive injuries in explosive attack site were systematically expounded.

Objective To summarize the experience of diagnosis and treatment of solid-pseudopapillary tumor of pancreas (SPTP).Methods The clinical data of 15 patients with SPTP who were treated in our hospital from March,2003 to March,2006 were analyzed retrospectively.Results The 15 cases were all women, and the average age was 29.4 years.The chief manifestations were abdominal mass, abdominal pain or other abdominal discomfort. None of the 15 cases had history of pancreatitis or abdominal trauma, and no long history of drinking or smoking. Six cases were negative for CEA,CA50,CA199,CA125 and other tumor markers.Solid and solid-cystic masses in pancreas or solid and solid-cystic tumors in retroperitoneum were found both by B-mode ultrasonography and CT examinations. Preoperative fasting blood sugar was within normal limits. The tumor in 8 cases was located in the pancreatic head, in 6 cases was in the body and tail of pancreas, and in 1 case was in the neck of pancreas. The diameter of the tumors was 2.5-10 cm. No metastasis was found in the abdominal cavity or liver. Local excision was performed in 6 cases, distal pancreatectomy was performed in 5 cases, including 2 cases combined with splenectomy, and pancreaticoduodenectomy was performed in 3 cases, segmental pancreatectomy was performed in one patient with tumor in the neck of pancreas. The 15 cases showed typical pathologic manifestation of SPTP by microscopy. At followed up for 16-52 months, no evidence of recurrence or metastasis in these cases was found.Conclusions Solid-pseudopapillary tumor of pancreas primarily affects young women, and it may be located in any part of pancreas. Surgical resection is recommended as the treatment of choice, and the prognosis is good.

Objective To investigate error-related negativity characteristics of patients with internalizing mental disorders and its diagnostic value.Methods Fifty-five patients met with DSM-Ⅳ anxiety or depression criteria were sampled from outpatients and inpatients,and 20 normal adults were selected as control group.With Eriksen and Simon tasks as evoked stimuli,error-related negativities of each participant were acquired using Canada Stellate-64 channel EEG/ERP Instrument and BESA5.2 software,and the data of general information,clinical symptoms,reaction time,and error rate were collected.Results (1) Correct reaction time ((377.5±98.8) ms)and errors reaction time ((337.3±96.6)ms) of patient group were significantly longer than those of control group ((288.5±105.9)ms and (281.9±94.3)ms) on the Eriksen task and Simon task,and there were no significantly group differences on other indicators (P＞0.05).(2) ERN amplitudes at 6 sities (Fz =(4.97±5.74) μV,F3 =(5.50±4.59) μV,F4 =(6.26±6.79) μV,Cz =(4.74±5.51) μV,C3 =(5.44±4.8) μV,C4 =(5.50± 5.28) μV) of patients group were significantly greater than those of control group ((1.17± 1.47)μV,(2.22±3.95) μV,(3.40±2.55)μV,(2.28± 1.41) μV,(3.05±2.47) μV,(3.16±2.51) μV) on the Eriksen task.ERN amplitudes at Fz((5.43±6.61) μV) and F4((6.00±5.50)μV) of patient group were significantly were greater than those of control group ((2.05 ±2.34) μV,(3.09±2.86)μV) on the Simon task,and there were no significantly group differences on the ERN amplitudes at other sites (P＞0.05).(3) ERN latencies ((87.6±34.4) ms and (84.9±34.8) ms) of patient group at Fz and F4 were significantly longer than control group ((54.0±46.5) ms and (36.3±42.4)ms) on the Simon task,and there were no significantly group differences on the ERN latencies at other sites (P＞0.05).Conclusion The increased error related potentials suggest that patients with internalizing mental disorders has an internal error monitoring defects,and ERN might be a useful endophenotype for internalizing mental disorders.

Objective To obtain a single toxin component from the jellyfish Cyanea capillata and provide a foundation for the further study on bioactivity and function of the serine proteases from C.capillata.Methods Primers designed with restriction enzyme were used to amplify the coding region of cDNAs (CcSP1, CcSP2 and CcSP3).PCR fragments were ligated with the pET-24a( +) vector to construct the recombinant plasmids (pET24a-CcSP1, pET24a-CcSP2 and pET24a-CcSP3).After screening and identification,the recombinant plasmids were transformed into the Rosetta (DE3).plysS for protein expression.After induction with IPTG, SDS-PAGE and Western-blot were used to detect the expression of the recombinant proteins.Results SDS-PAGE showed that the proteins of rCcSP1, rCcSP2 and rCcSP3 were expressed in a single band at about 34 kDa, 42 kDa and 42kDa, respectively.Western-blot detection with anti-His antibody further confirmed that these recombinant proteins were His-tagged CcSP1, CcSP2 and CcSP3 fusion protein were obtained.Conclusion Prokaryotic recombinant plasmids of C.capillata serine proteases are contructed and recombinant proteins are obtained, which establishes the foundation for future study on the function of serine proteases from jellyfish.

Objective To study the value of Geneva score assessment combined with simplified rapid plasma D-dimer assay(SRPDDA)for early diagnosis of pulmonary embolism(PE).Methods Clinical data of 658 suspected cases of PE hospitalized at Shanghai Pulmonary Hospital during January 1,1995 to November 30,2007 were retrospectively analyzed.The patients were divided into three categories based on their Geneva score assessment,highly suspected with equal to or greater than 9 scores,intermediately suspected with 5-8 scores and mildly suspected with equal to or less than 4 scores,respectively.Clinical diagnosis for PE of the patients was made based on their probability Geneva score developed by Wicki,SRPDDA,and both Geneva score assessment and SRPDDA,respectively,and compared with results of lung imaging.Results Finally,PE diagnosis was established in 267 cases.Sensitivity for Geneva score and SRPDDA to diagnose PE was 88.4%and 90.3%,with negative predictive value(NPV)of 88.9%and 90.2%and Youden Index(YI)of 51.7%and 51.4%,respectively.And,sensitivity of both Geneva score assessment combined with SRPDDA wag 97.8%,with NPV and YI of 97.3%and 53.8%,respectively,with a likelihood ratio(LR)for both positive test results of 2.62 and a LB for both negative test results of 0.04,respectively.Conclusions As a routine and non-invasive diagnostic test,Geneva score assessment,as well as SRPDDA,play a screening role in early diagnosis for PE,which can help to save unnecessary cost of imping,and complement each other so to improve accuracy of diagnosis and reduce misdiagnosis.

50%) of the stent at 2 weeks follow-up,so did 3 cases at 12 weeks follow-up,and the total stenosis rate was 37.5% and 5 cases manifested full endothelialization(3 different locations of the sample all manifested full endothelialization) ,3 cased manifested partial endothelialization(at least 1 location of the sample didn't show full endothelialization) ,and the two terminal parts were easier to get endothelialization than the central part.Before and after the stent implantation,hepatic function of all cases didn't demonstrate any obvious changes.Conclusions Balloon-expandable covered stent can be implanted in canine hepatic artery successfully,with good apposition ability,full endothelialization,and no influence on hepatic function.