Objective:To establish the quality control for Yangxueyin oral liquids. Methods:TLC was applied to identify Angeli-cae Sinensis Radix and Fructus Ziziphi Jujubae. The content of astragaloside A in Yangxueyin oral liquids was determined by HPLC-ELSD. A Luna C18(250 mm ×4.6 mm,5 μm)column was used, the mobile phase was acetonitrile-water (38∶62)with the flow rate of 0. 8 ml·min-1 , and the column temperature was 40℃. The temperature of the drift tube was 85℃, and the flow rate of the carrier air was 2.0 L·min-1. Results: The linear range of astragaloside A was 0.522-4.176 μg(r =0.999 8). The average recovery was 97. 9% with RSD of 1. 03% (n=6). Conclusion:The method is convenient, sensitive and accurate, which can be used in the quality control of Yangxueyin oral liquids.

Objective To determine the relation between the expression of urocortin and corticotropin-releasing hormone receptor 2? (CRH-R2?) in the placenta and pathogenesis of preeclampsia. Methods Placentas were collected from 20 pregnant women with preeclampsia as study group and 20 normal pregnant women as control group. Urocortin mRNA and CRH-R2? mRNA were measured by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Urocortin peptide was measured by immunohistochemistry. Results (1) The mRNA expression of urocortin was significantly higher (P

Background and purpose:Axillary lymph node metastasis of breast cancer has an important significance in prognosis and treatment of breast cancer. This study was to investigate the correlation between axillary lymph node metastasis and ultrasonographic characteristics of axillary lymph node combined with immunohistochemistry in breast cancer patients.Methods:A total number of 366 breast cancer patients were selected in this study. Seven hundred and twenty-eight axillary lymph nodes were collected. With ultrasonography, the maximum cortex thickness, the ratio of the height to the length, the ratio of the cortex to the medulla and blood lfow of axillary lymph nodes were observed, in order to study the correlation between these indicators and axillary lymph node metastasis combined with the postoperative immunohistochemical results.Results:According to univariate analysis, axillary lymph node maximum cortex thickness, the ratio of the height to the length, characteristics of blood flow and the positive expression rate of p53 were related to axillary lymph node metastasis (P<0.05). Multivariate logistic regression analysis and receiver operating characteristic (ROC) curve showed that axillary lymph node maximum cortex thickness was the best indicator to determine axillary lymph node metastasis. The positive expression rate of p53 in patients with maximum cortex thickness >3 mm (42.78%) of axillary lymph node was signiifcantly higher than that in patients with maximum cortex thickness≤3 mm (25.82%) (P<0.01).Conclusion:Ultrasonographic characteristics of axillary lymph node and immunohistochemistry method are closely correlated with axillary lymph node metastasis in patients with breast cancer, which is important in diagnostic and treatment in clinic.

Objective To examine the expression of urocortin mRNA during labor and the effect of uroco rtin on myometrial contractility, and to investigate its role in the onset and p rogress of labor. Methods (1) Semi-quantitative reverse transcription-polymerase chain reaction (RT -PCR),using ?-actin as internal standard was applied to determine the levels of urocortin mRNA in human placenta and myometrium from the group of cesarean se ction before (10 cases) and during (10 cases in latent phase and 10 cases in act ive phase) labor.(2) The isolated myometrial strips of pregnant women (n=24 ) were prepared.The effects of urocortin with or without prostaglandin F 2? (PGF 2?) and oxytocin on myometrial contractility were evaluated by area s under the curve. Results (1) Semi-quantitative RT-PCR showed that the expression level of urocortin mRN A in placenta and myometrium after the onset of labor were higher than before la bor (1.23 ?0.52, 1.32?0.22; 0.83?0.38, 0.94?0.13, respectively, P0.05). Conclusion The study indicates urocortin may indirectly m odulate myometrial contractility during labor.

Objective To observe the expression of HER-2 and TOPO-Ⅱα in ovarian epithelial cancer,analyze the correlation between their expression and provide theoretical basis for clinical diagnosis,prognosis and treatment. Methods Expression levels of HER-2 and TOPO- Ⅱα in 10 normal ovarian tissues,20 benign tumors and 58 cases of ovarian epithelial cancers were detected by immunohistochemical method, and their correlations with pathological features were analyzed. Results The positive expression rate of HER-2 in normal ovarian and benign tumor tissues were significantly lower than ovarian epithelial cancers respectively ( 10. 0% , 15.0% VS 46. 6% ;P ＜ 0. 05 ). The positive expression rate of TOPO- Ⅱα in ovarian epithelial cancers was significantly higher than normal and benign epithelial ovarian tumor tissue (53.4% vs 10. 0%, and 15.0%,Ps ＜ 0. 05 ), but we did not find significant difference in the comparison between normal and benign epithelial ovarian tumor tissue ( Ps ＞ 0. 05 ). The expression of HER-2 and TOPO- Ⅱα were significantly correlated with clinical stages, histological differentiation of tumor cells (Ps ＜ 0. 05 ) ,but there were no correlations between the age or histological type. In ovarian cancer tissues, a positive correlation between the expression of HER-2 and TOPO- Ⅱα was observed ( r = 0. 324, P ＜ 0. 05 ) . Conclusion The overexpression of HER-2 and TOPO- Ⅱαplay an important role in ovarian carcinogenesis and development. The expression of HER-2 is positively correlated with TOPO- Ⅱα in ovarian epithelial cancers. Coexpression of the two moleculars may be involved in the development and progression of ovarian epithelial cancer, which should be further studied.

Objective To analyse the expression of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in ovarian epithelial cancer and to investigate the relationship between PTEN and clinical pathology and prognosis of ovarian epithelial cancer.Methods Expression of PTEN in 10 normal ovarian tissues,20 benign tumors and 60 cases of ovarian epithelial cancers were detected by immunohistochemical method.Results The positive expression of PTEN in normal ovarian and benign tumor tissues were significantly higher than that in ovarian epithelial cancers (100％ (10/10) vs 80.0％ (16/20) vs 53.3％ (32/60),x2 =7.778 and 4.444 respectively,P ＜ 0.05).The expression of PTEN was correlated to clinical stage,histilogical grade,presence and metastasis of lymph node (x2 =4.339;6.465 ;3.896;10.452;P ＜0.01),but there was no correlation to age,histological type and ascites (x2 =0.004 ;0.388 ; 1.057 ; P ＞ 0.05).Conclusion The expression of PTEN is related to ovarian carcinogenesis and development and may has great value in clinical diagnosis and prognosis of ovarian carcinogenesis.

BACKGROUND: Wnt signaling pathway plays an important regulative role in the embryonic development processes. Accordingly, it is of great significance to establish the cell model of Wnt signaling pathway so as to conduct study on it. OBJECTIVE: To establish Wnt signaling pathway cell model by transfecting L-M (TK-) cells with Wnt3a eukaryotic expression plasmid, and to investigate the effect of canonical Wnt signal pathway on the β-catenin subcellular distribution. METHODS: The eukaryotic expression plasmid pgk-Wnt3a-pcDNA3.0 after amplification was digested by restriction endonuclease first. Then it was transfected together with the control plasmid pgk-neo-pcDNA3.0 into L-M (TK-) cells via lipofection, after which the cell colony was screened by G418 for amplification. RT-PCR was used for detecting the expression products and the indirect immunofluorescence assay for observing the effect of Wnt3a on the β-catenin subcellular localization of L-M (TK-) cells. RESULTS AND CONCLUSION: The Wnt3a plasmid was verified by endonuclease digestion to have produced the expected plasmids after amplification. According to the RT-PCR detection to the 10 stably-transfected cell colonies achieved by 3 weeks of G418 screening, it was seen, on the L-Wnt3a cDNA, a strip of bright band of 320 bp in length, which showed that the products of amplification were exactly the expected fragments and that the Wnt3a plasmid was expressed on mRNA transcriptional level after being transfected with L-M (TK-) cells. In contrast, no expected band was found on the cDNA of L-M (TK-) calls transfecting the control plasmid. In addition, the immunofiuorescence assay detection showed that the protein expression of Wnt3a was found in the cytoplasm of the L-M(TK-) cells tranfecting Wnt3a plasmid, while for those transfecting the control plasmid, it was opposite. β-catenin, as showing by bright red fluorescence, was found to concentrate and enter into the nucleus of the L-M (TK-) cells transfecting Wnt3a plasmid, while for those transfecting the control plasmid, it was opposite. Cell model with continually activated Wnt signaling pathway is established. The stable expression of Wnt3a in L-M (TK-) cells transfected with pgk-Wnt3a-pcDNA3.0 is obtained. The expression of Wnt3a is able to promote the transfer of β-catenin from cytoplasms into nucleus in L-M (TK-) cells.

OBJECTIVETo evaluate the long-term clinical effect of dual anti-collagen membranes in guided tissue regeneration (GTR).

METHODSThis randomized clinical trial included 26 teeth in 24 patients, presenting a total of 31 lesions consisting of intrabony defects and furcation defects. Twenty-six teeth were divided into two groups and treated by GTR with dual anti-collagen membranes and atelocollagen membranes, respectively. At baseline, 6 months, 1, 3 and 6 years, the following parameters were recorded: clinical attachment level, probing depth, gingival recession and the quantity of alveolar bone analyzed by computer assisted densitometry image analysis (CADIA).

RESULTSAt 1 year after GTR surgery, the gain of clinical attachment in dual anti-collagen membranes group was (3.93 ± 1.74) mm, compared with (2.25 ± 1.90) mm in atelocollagen group (P = 0.044). The increasing of the value of CADIA in dual anti-collagen membrane and atelocollagen group were (53.14 ± 21.35) and (32.96 ± 17.97), P = 0.031. At 3 and 6 years, clinical parameters remained basically stable in both groups, compared to that at 1 year after surgery.

CONCLUSIONSThe regeneration of periodontal tissues obtained by GTR with dual anti-collagen membranes could be maintained on a long-term basis.

Adult ; Alveolar Bone Loss ; surgery ; Bone Regeneration ; Collagen ; Densitometry ; methods ; Dental Plaque Index ; Female ; Follow-Up Studies ; Furcation Defects ; surgery ; Guided Tissue Regeneration, Periodontal ; methods ; Humans ; Image Interpretation, Computer-Assisted ; methods ; Male ; Membranes, Artificial ; Middle Aged ; Periodontal Attachment Loss ; surgery ; Periodontal Index ; Young Adult

Objectlve To explore the effect of Qibai Pingfei capsule on the right ventricular hypertrophy index (RVHI)of rats with phlegm and blood stasis of COPD.Methods 60 rats were randomly divided into 6 groups,a control group,a model group,a Ligustrazine group,a Nifedipine group,a high dose Qibai Pingfei capsule group and a low dose Qibai Pingfei capsule group.Composite factors method was adopted to establish phlegm and blood stasis COPD rat model.At the same time of modeling.Qibai Pingfei capsule,Ligustrazine,Nifedipine were also given to these rats.Observed the changes of RVHI in each group.Results RVHI did not show statistic difierence between high dose of the Qibai Pingfei capsule group and the control group(P＞0.05).and between the Nifedipine group and the model group(P＞0.05).RVHI manifested significant diffbrencc between the Ligustrazine group and the low dose Qibai Pingfei capsule group(P＜0.01),and between the Ligustrazine group and the control group(P＜0.01).Conclusion High dose Qibai Pingfei capsule can effectively prevent the right ventricular hypertrophy of model rats with the phlegm and blood stasis of COPD.Ligustrazine has certain effects but not as good as high dose Qibai Pingfei capsule.Nifedipine can not prevent the right ventricular hypertrophy of model rats.

Objective: To investigate the relationship between the melatonin and the development of testicle in rabbits. Methods: Thirty male rabbits (unreach puberty) were randomly divided into 2 groups:15 were actively immunized with melatonin and complete antigen, which was successfully synthesized through Mannich reaction by combining melatonin with BSA, the other 15 were taken as controls.The specific antibodies of rabbits were detected by ELISA and RIA.The serum testosterone(T) and luteinizing hormone(LH) were detected by RIA, the mass of testicle or epididymis and the number of sperm were measured. Results: The serum T and LH concentration in experiment group were significantly lower than that in the control group.The mass of testicle or epididymis and the number of sperm in experiment group were also significantly lower than that in the control group. Conclusion: The results suggest that the immunization of melatonin can block the development of testicle in male rabbits.