To build a reversed phase ion-pair chromatography to determination content of Dencichine from Panax notoginseng. Using Tetrabutyl ammonium hydroxide ions by the combination of reagent and HPLC method without derivatization to test the content of dencichine directly. The optimum conditions of supersonic extraction were solid-to-liquid ratio 1: 20, Continuous ultrasonic extraction: twice, each time 15 minutes; 3,500 r · min⁻¹, then centrifuging 15 minutes. Dencichine in different age, place, part and the different Processing mode were examined. The method is simple with sound separation degree and stability, which can facilitate the determination of dencichine content directly and provide the basis in quality standard of raw material.
Amino Acids, Diamino ; analysis ; Chromatography, Reverse-Phase ; methods ; Drugs, Chinese Herbal ; analysis ; Panax notoginseng ; chemistry ; Plant Roots ; chemistry

imaging features of myositis ossificans have some characteristics. Misdiaguosis could be avoided when the disease was evaluated with the course.

[Summary] The clinical data of 3 inpatients clinically diagnosed as Gitelman syndrome ( GS ) were collected. The genomic DNA was isolated from the peripheral blood and the primers were designed to amplify all the exons and flanking introns in the SLC12A3 and CLCNKB genes by PCR. Direct sequencing of PCR products in the two genes was performed in all patients. Three patients manifested with recurrent hypokalemia, hypomagnesemia, hypocalciuria, hypochloremic metabolic alkalosis, but normal blood pressure. Gene sequencing results showed that one novel mutation p. L891V was identified in SLC12A3 gene in case 2. Seven and 12 types of polymorphic loci in the CLCNKB gene were found in case 1 and case 3, respectively. However, mutations were not found in the SLC12A3 and CLCNKB gene.

BACKGROUND: Anterior cervical discectomy and fusion (ACDF) is the gold standard for degenerative cervical disease,which would be replaced by cervical disc arthroplasty (CDA) with the wide application of CDA. But, the mid- and long-term outcomes of ACDF versus CDA remain controversial.OBJECTIVE: To compare the mid- and long-term outcomes of ACDF and CDA in the treatment of single-level cervical spondylosis.METHODS: PubMed, Medline, EMbase, Cochrane, CBM, CNKI, VIP and WanFang databases were searched for randomized controlled trials addressing CDA versus ACDF for single-level cervical spondylosis published before August 2016. The quality of trails was strictly evaluated, the data were extracted and a meta-analysis was performed on ReviewManager5.3 software.RESULTS AND CONCLUSION: (1) Totally 15 randomized controlled trials involving 2781 patients were included, with 4-10 years of follow-up. (2) Meta-analysis results showed that compared with ACDF, CDA had better SF-36 scores,larger range of motion at operation level, lower the Neck Disability Index, and Visual Analogue Scale scores for arm pain,lower reoperation rate at operation level and adjacent level at mid- and long-term follow-up. (3) The Visual Analogue Scale scores for neck pain, neurologic success and all-complication rate did not differ significantly between two groups.(4) These results manifest that CDA is superior to ACDF in the mid- and long-term outcomes for single-level cervical spondylosis; however, further large-scale, multi-center and high-quality randomized controlled trials will be necessary.

In order to establish a method for the determination of the sterols of the oil in the freeze-dried acai (Euterpe oleracea Mart.) and to evaluate its antioxidant activities, a saponification/extraction procedure and high performance liquid chromatography (HPLC) analysis method were developed and validated for the analysis of phytosterols in PEE (Petroleum ether extract). Separation was achieved on a Purosper STAR LP C18 column with a binary, gradient solvent system of acetonitrile and isopropanol. Evaporative light scattering detection (ELSD) was used to quantify β-sitosterol and the total sterols. Peak identification was verified by retention times and spikes with external standards. Standard curves were constructed (r = 0.999 2) to allow for sample quantification. Recovery of the saponification and extraction was demonstrated via analysis of spiked samples. The highest content of total sterols is β-sitosterol. The antioxidant activities of the extracts were evaluated using the total oxyradical scavenging capacity assay (TOSC assay). The result showed that the PEE exhibited significant antioxidant properties, sample concentration and the antioxidant capacity had a certain relevance.
Antioxidants ; analysis ; Arecaceae ; chemistry ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; analysis ; Phytosterols ; analysis ; Sitosterols ; analysis

Objective To explore the concept,denomination and technique of miniature tissue transplantation in hand clinic. Methods In 537 cases,local skin or composite tissue defect in 756 thumbs or fingers were repaired with 4 kinds of miniature island flap and 10 kinds of miniature vascularized composite tissue.Some of them were created in clinic practice.The precess,main points and indication of the operation were expounded and compared. Results In 639 fingers covered with miniature island flap,the transferred flaps were surviverd completely in 621 fingers(97.2%),survivered partly in 11 fingers(1.7%)and necrosised in 7 fingers(1%).In 117 fingers repaired with miniature vascularized composite tissue,all the transplanted tissues were survivered.In all the injuried fingers,beautiful outlook were recovered and no fat and clumsy were found,two-point difference was 6-8 mm,effctive sensibility and good function were restored,and all the patients were satisfied. Conclusion Miniature tissue transplantation were good methods to cover the local defect in fingers or thumbs,worth of generalization and aplication in hand clinic,and have an important significance for improve the trauma repair quality and treatment level in hand surgery.

Objective To explore the superiority of dual-phase contrast-enhancement multi-slice computed tomography (MSCT) in observing intracranial tuberculosis.Methods Thirty patients with intracranial tuberculosis,admitted to our hospital from January 2003 to November 2011,were chosen in our study; regular 16-slice spiral CT scan was performed,and then,dual-phase MSCT was performed as follows:contrast-enhanced scan was performed 25 seconds after arrival of contrast material on vascular phase,and 5 minutes after that (lag phase),conventional scanning was performed.According to the different lesions and morphologies of the tuberculosis,they were divided into three types:meningeal thickening,meningeal tuberculoma and parenchymal tuberculoma; the lesion sizes and edge definition and the relationship of the lesions with the surrounding vascular lesions in these three types were scored and calculated,and the differences of the image quality were compared at the vascular phase and lag phase.Results A total of 526 lesions in 30 patients were found,including 22 with meningeal thickening,235 with meningeal tuberculomas/tubercles,and 269 with parenchymal tuberculomas/tubercles.As compared with these three types at the vascular phase (0.36±0.49,0.36±0.52 and 0.41±0.53; 0.00,0.27±0.45 and 0.12±0.32; 1.09±0.68,1.22±0.74 and 1.27±0.75),these three types at the lag phase had significant differences in the scores of lesion sizes (1.64±0.58,1.64±0.58 and 1.59± 0.60) and lesion edge definition (2.00,1.73±0.49 and 1.88±0.34) and the relationship of the lesions with the surrounding vascular lesions (1.82±0.39,2.00±0.06 and 2.00±0.06,P＜0.05).Conclusion Images in the lag phase have advantages on diagnosis of intracranial tuberculosis.

Objective To establish a high performance liquid chroma-tography-tandam mass spectrometry ( HPLC-MS/MS) method for de-termining the concentration of sunitinib and N -desethyl sunitinib (SU12662) in human plasma.Methods Sunitinib, SU12662 and in-ternal standard sunitinib -D10 were extracted from plasma with one -step protein precipitation and the stereoselective analysis of sunitinib and SU12662 was achieved on an Agilent ZORBAX Extend C18 (2.1 mm ×75 mm ×3.5 μm) with gradient elution by a mobile phase consisting of wa-ter containing 0.1%formic acid and 95%acetonitrile.Electrospray ioni-zation source was applied, and multiple reaction monitoring mode was operated in the positive mode with the monitor ions at m/z 399.3→326.1 for sunitinib, m/z 371.3→283.1 for SU12662 and 409.3→326.2 for sunitinib-D10.Results The calibration curve for plasma sunitinib was linear in the range of 0.5-200 ng? mL-1 ( r=0.998 7) , the lower limi-tation of quantification was 0.5 ng? mL-1 , while the accuracy was ranged from 0.57% to 2.87%, the average recovery rate was ranged from 92.75%to 98.62%,and intra-and inter-day RSD were ranged from 0.91%to 1.92%and 5.40%to 7.87%, respectively.The calibration curve for plasma SU12662 was linear in the range of 0.25 -100 ng? mL-1 ( r =0.999 9 ) , while the accuracy was ranged from -2.08% to 2.80%, the average recovery rate was ranged from 96.23%to 101.12%, and intra-and inter-day RSD were ranged from 0.46%to 2.46%and 5.84%to 9.75%, respectively, the lower limitation of quantification was 0.25 ng? mL-1 .Conclusion The HPLC-MS/MS method was accurate, sensitive, specific, and could be used in the determination of sunitinib in plasma and the clinical study of its pharmacokinetics.

Objective To determine the bicalutamide concentration in human plasma by established HPLC-MS/MS method.Methods The plasma was treated with protein precipitation,Bicalutamide-d4 was used as internal standard,chromatographic column:Agilent ZORBAX SB-C18 column (2.1 mm ×S0.0 mm,5 μm),column temperature:20 ℃,mobile phase:methanol-0.1％ formic acid and 2 mmol · L-1 ammonium formate,isocratic elution,flow rate:0.35 mL · min-1,mass concentration of the sample was measured with negative ion scan and multiple reaction monitoring mode.The specificity,lower limit of quantitation and standard curve,precision and recovery rate and stability as well as the matrix effect were investigated.Results The standard curve of bicalutamide was y =6.82 × 10-3x + 1.33 × 10-2 (r =0.999 1),and has good linear relationship in 10-1500 ng · mL-1,the lowest concentration was 10 ng · mL-1.Intra-day and inter-day RSD of blood samples were less than 15％,the average recovery was ＞ 95％,and stability was good.Conclusion The method was simple,rapid,sensitive and accurate,specific,suitable for the determination of bicalutamide concentration in human plasma.

Objective To investigate the effects of ATP-binding cassette sub-family G member 2 (ABCG2) and CYP3A4 gene polymorphisms on the pharmacokinetics of bicalutamide and compare the pharmacokinetic properties of different formulations of bicalutamide (capsules and tablets).Methods The subjects were randomly divided into two groups:a single dose of oral test preparation or reference preparation 50 mg,the high performance liquid chromatography-mass spectrometry was used to determine of plasma concentration of bicalutamide,the Phoenix WinNonlin 6.4 was used to calculate pharmacokinetic parameters,and bioequivalence evaluation.Analysis the effects of ABCG2 gene polymorphisms on the pharmacokinetics of bicalutamide by SPSS.Results The main phannaeokinetie parameters of the bicalutamide in the test and reference preparation were as follows:Cmax were (900.00 ± 159.20) and (902.40 ± 146.10) ng· mL-1;tmax were (26.40 ± 9.60) and (35.30 ± 19.80) h;AUC0-t were (1.96 × 105 ±3.28 × 104) and (2.02 × 105 ±4.84 × 104) ng · h · mL-1;AUC0-∞ were(2.03 × 105 ±3.62 × 104)and(2.11 × 105 ±5.63 × 104)ng · h · mL-1.The 90％ confidence interval of Cmax was 88.93％-111.39％;the 90％ confidence interval of AUC0-t was 85.97％-110.76％,the AUC0-∞ 90％ confidence interval was 85.18％-111.51％.There was a significant difference of AUC0-t between CC,AA and CA group (P ＜0.05) and a significant difference of AUC0-∞ between CC and AA group (P ＜ 0.05).There was significant difference of CL between CC and AA group (P ＜ 0.05).Conclusion The pharmacokinetic properties of the two preparations are similar.