MicroRNA (miRNA) is a class of endogenous non-coding RNA which found in eukaryotes and has regulatory function.Its size is about 20 to 25 nucleotides.Recent studies found that miRNA is closly related to the EBV,LMP1,signal transduction pathway,tumor-associated gene network,cell mitosis,tumor angiogenesis,tumor invasion and metastasis of nasopharyngeal.Therefore,studing the relationship between miRNA and nasopharyngeal will help to deepen the understanding of its pathogenesis,and also will provide new inspiration and basis for clinical diagnosis and treatment.

A Review] The biological characteristics of viral L6565 leukemia cell clone were as follows: (1) The chromosome counts varied 38～114 , and stem cells were 42; (2) Virus particles type A and type C found in the cytoplasm of clone cells; (3) X-C assays were positive, c- myc and c- fos gene overexpressed in clone cells; (4) Differential markers CD4, CD8, CD45R were negative, CD45RO ? were positive; (5) The supernatant of clone cells could induce T or B lymphocytic leukemia/lymphoma and granulocytic leukemia in SSB strain mice. The leukemogenic effect of concentrate supernatant was stronger than non-concentrate supernatant( P

BACKGROUND: Xiongbitong capsule (capsule) increases blood flow in myocardial ischemic area in patients with coronary heart disease, lessens the size of myocardial infarction and improves ischemic S-T alternation in electrocardiogram (ECG).OBJECTIVE: To observe the effect of the capsule on NO metabolism on arterial wall in rabbits and explore the improvement of such drug on myocardial blood flow and protection on myocardium.DESIGN: Randomized group division and control experiment were designed.SETTING: Department of First Internal Medicine, Hospital of Traditional Chinese Medicine, Weifang, Shandong.MATERIALS: The experiment was performed in Teaching-Research Room of Physiology of Weifang Medical College from April 2003 to February 2004. Totally 30 healthy adult rabbits were employed, of either sex and randomized into 3 groups, named normal control, model group and treatment group, 3 rabbits in each one.METHODS: ① Preparation of hyperlipemia (HP) model: In normal control, common granular forage was used. In model group, modeling forage was applied (common granular forage + 3% pig oil + 1% cholesterol powder). In treatment group, besides modeling forage, at the end of the 6th week, the capsule [composed of taizishen (Radix Pseudstellariae), yunling (Poria), gualou (Frnctus Trichosanthis), qingxia (Rhizoma Pinelliae), juhong (Exocarpium Citri Grandis), shichangpu (Rhizoma Acori Graminei), yujin (Radix Curcumaea), zhifuhua (Flos Inulae), jiangxiang (Lignum Dalbergiae Odoriferae), danggui (Radix Angelicae Sinensis), maidong (Radix Liriopis Platyphyllae) and wuweizi (Frnctus Schisandrae).] was administrated every day for gastric perfusion at 1 pill/kg with 10 mL physiological saline for 3 weeks. The intervention was applied totally for 8 weeks. ② Nitrieoxide synthase (NOS) and NO metabolic product kits were provided from Radiation Medical Institute of Military Academy of Medical Sciences. The operation was based on the instrnction. NOS activity was calculated and expressed by NO amount (nmol) produced in one-gram tissue per minutes.Spectrophotometer was used to measure the optic density of sample at 545nm. Sodium nitrite solution was used to prepare standard curve. The concentration of serum NO metabolic products, NO2- and NO3- was expressed with μmol/L. ③ T test was applied for difference comparison among measurement data.MAIN OUTCOME MEASURES: Comparison of NOS activity and concentration of serum NO metabolic products in vessel-wall tissue in eachgroup.RESULTS: Totally 30 rabbits all entered result analysis. For NOS activity and concentration of serum NO metabolic products in arterial-wall tissue,the results in model group and treatment group were lower remarkably than the control (P ＜ 0.05-0.01) and the results in treatment group were higher remarkably than model group (P ＜ 0.05).CONCLUSION: ① NO system of arterial wall is damaged in HP rabbits and NO metabolic level is decreased in serum. ② Xiongbitong capsule improves the above situation and brings the resistance of myocardial ischemia and protection of myocardium into play.

Objective To investigate effect of polymorphism of apolipoprotein E (APOE) gene on early apoptosis of astrocytes after hypoxic injury.Methods Astrocytes separated from APOE wild mice and APOE transgenic mice (ε3,ε4) were primarily cultured,and then purified and identified.Models of astrocyte hypoxic injury were set up by hypoxia.Morphological changes of astrocytes and mitochondria were observed by transmission electron microscope.Early apoptosis rate and changes of mitochondrial membrane potential were detected by flow cytometry.Results Cell foot process tumidness and mitochondria with irregular outline,vacuoles and irregular cristae were observed in each group by electron microscopy at six hours after hypoxia.There were no significant differences of cellular form changes among groups.Early apoptosis rate and decreasing degree of mitochondrial membrane potential in APOFε4 group were significantly higher than those in APOEε3 group and APOE wild group (P ＜ 0.05).Conclusion Compared with astrocytes from APOEε3 group and APOE wild group,mitochondrial membrane potential in astrocytes from APOEε4 group at early period after hypoxia declines more significantly,as may be one of causes for more astrocyte apoptosis.

After the problems found in academic information push service and information literacy training of sub-ject librarians in Medical Library of Chinese PLA were analyzed , the concrete contents of subscribing personal RSS products in a certain institute of Academy of Military Medical Sciences and the feedback of its users were described with suggestions put forward for deepening the academic information push service, such as establishing personal RSS account for research rooms and implanting certain RSS subscription contents into the subject service platform.

AIM:To investigate the impact of various levels of glucose on endothelial progenitor cells (EPCs) proliferation, senescence, and nitric oxide (NO) secretion,and the effect of insulin under high glucose conditions.METHODS: Mononuclear cells were collected from rat bone marrow by density gradient centrifugation, cultured with medium 199, and identified to be EPCs at 7th day by flk-1 and AC133 double staining. EPCss were harvested and incubated with glucose (5, 10, 20, 40 mmol/L) or insulin (0.1, 1, 10, 100 nmol/L) under high glucose conditions for 24 h or 7 days. Proliferative capacity, senescence level and NO secretion (after 24 h of incubation) were subsequently determined.RESULTS: High glucose (40 mmol/L) markedly inhibited EPCs proliferation, accelerated EPCs senescence, and decreased NO production (all P

Objective To explore the dysfunction of dendritic cells (DC) related to TGFβ reversed after blocking the TGFβ signal pathway by recombinant adenovirus vector encoding for Smad7.Methods Smad7 by recombinant adenovirus vector was transfected into dendritic cells.Expression of immunologic phenotypes was detected by FCM,and CTL activity induced by DC was compared.Results The DC modified with Smad7 still expressed high adhesiveness factor related to maturation even if existing exogenous TGFβ1,which was significant statistically compared with DC transfected with control adenoviral vector (P ＜0.01).Even if existing exogenous TGFβ1,the DC modified with Smad7 pulsed with soluble antigen associated with Lewis pulmonary carcinoma could still induce potent CTL activity against Lewis pulmonary carcinoma,which showed significant difference with DC-Ad-c (P ＜0.01).Conclusion The inhibitory effects on function of DC of TGFβ may be reversed by blocking the Smad signal of TGFβ pathway.

Objective To evaluate the incidence and epidemiologic characteristics of Kawasaki disease (KD)in Nanjing.Methods We reviewed the medical records and reports of all patients admitted to Nanjing Chil-dren′Hospital with KD diagnosed during 3 -year periods from 2012 to 2014.Results We studied 1 446 inpatients diagnosed with KD from 2012 to 2014.There were 1 135 cases(78.5%)with complete KD.The ratio of male to female was 1.7241.The disease occurred in all of the seasons,but the peak was from spring to summer.Age at onset ranged from 1 month to 14 years old,and the peak age group was 1 year old.Fever(99.45%)was the most common clinical symptom,followed by conjunctive congestion(93.15%),rash(73.32%),oral changes(51.76%), extremities desquamate(75.08%)and lymphadenopathy(45.89%).There were 131(9.05%)patients with coronary aneurysm,the ratio of male to female was 3.02  1.14 patients (0.97%)developed recurrent KD.Conclusion Patients with KD has become more and more in Nanjing,and clinical doctors should improve the understanding of KD. Early diagnosis and treatment is very important to reduce the sequelae and reduce the mortality of KD.

Animals ; Catechin ; analogs & derivatives ; pharmacology ; Disease Models, Animal ; Fatigue ; metabolism ; physiopathology ; Learning ; drug effects ; Male ; Memory ; drug effects ; Rats ; Rats, Sprague-Dawley ; Receptors, N-Methyl-D-Aspartate ; metabolism

OBJECTIVETo investigate the effects of epigallocatechin gallate (EGCG)against exercise-induced fatigue in mice.

METHODSTotal 120 mice were randomly divided into three groups and tested separately. For each test, there were 30 mice subdivided into high dose (50 mg/kg . d EGCG) and low dose (10 mg/kg . d EGCG) groups as well as saline control group(1 ml/kg . d) with 10 in each. Burden swimming, running wheel endurance, stick climbing and hypoxia tolerance exercise were used to establish fatigue mice training model in three groups. And intraperitoneal injection with different doses of EGCG per day for consecutively 28 days and the mice in the control group were treated with normal saline. After the last each test, the blood lactic acid (BLA), blood urea nitrogen (BUN), blood lactate dehydrogenase (LDH), muscle glycogen (MG) and liver glycogen (LG) of each group of mice were determined.

RESULTSEGCG treatment groups(B and C)revealed a prolonged the mice survival time of burden swimming test, hypoxia tolerance, running wheel time and the ability of stick climbing(P < 0.05 or P <0.01), and increased LDH activity and MG and LG contents, reduced contents of BLA and BUN. High dose group had an obviously increase effect than lower dose group(P <0.05).

CONCLUSIONEGCG has significant effects against exercise-induced fatigue in mice.

Animals ; Blood Urea Nitrogen ; Catechin ; analogs & derivatives ; pharmacology ; Exercise Tolerance ; Fatigue ; drug therapy ; Glycogen ; metabolism ; L-Lactate Dehydrogenase ; blood ; Mice ; Physical Conditioning, Animal