Objective: To investigate the anticancer activity of histone deacetylase (HDAC) inhibitor trichostatin A (TSA) on osteosarcoma in vitro and its possible mechanism. Methods: Osteosarcoma MG-63 cells were cultured under the mimic hypoxia condition induced by desferrioxamine and treated with TSA in vitro. The effects of TSA on invasion of MG-63 cells was detected by Transwell migration assay. The effect of TSA on mRNA and protein expressions of HIF-1α and VEGF were tested by RT-PCR, Western blotting, and ELISA at various concentrations and different time points. Results: After treatment with TSA at 50, 100, 200, 300, and 500 nmol/L the invasion capability of MG-63 cells decreased significantly compared with the control group (P < 0.05). Mimic hypoxia induced the expression of HIF-1α and VEGF protein. TSA markedly inhibited the expression of HIF-1α and VEGF protein in a dose- and time-dependent manner. Conclusion: The histone deacetylase inhibitor TSA had an significant anticancer activity in vitro and inhibitory effects on the expression of HIF-1α and tumor angiogenesis. It might be a novel anti-cancer agent by targeting HIF-1α and subsequently inhibiting angiogenesis in osteosarcoma.

Objective: To investigate the expression of HIF-1α and its relationship with angiogenesis in osteosarcoma. Methods: Osteosarcoma MG-63 cells were cultured in vitro under hypoxia and mimic hypoxia conditions. Thirty paraffin-embedded osteosarcoma tissues and 20 fresh frozen osteosarcoma specimens were collected. The mRNA and protein expression of HIF-1α and VEGF were detected by RT-PCR, Western blotting, ELISA, and immunohistochemistry methods. The mean vessel density (MVD) were also calculated. Results: The mRNA level of HIF-1α had no change under hypoxia and minic hypoxia conditions, whereas the protein expression was increased dramaticaly. The mRNA and protein expression of VEGF was significantly increased under hypoxia and minic hypoxia conditions. The positive rate of HIF-1α mRNA (90%) and VEGF(100%) in 20 fresh frozen tissues were higher than those of the para-tumor tissues(P<0.05). The positive rates of HIF-1α and VEGF protein in paraffin-embedded osteosarcoma were 86.7% and 93.3%, respectively, significantly higher than those in the para-tumor tissues (6.7%,26.7%, P<0.05). Spearman related analysis demonstrated that the expression of HIF-1α (P = 0.005, r = 0.767) and VEGF(P<0.002, r = 0.701) had a positive relation with MVD. Conclusion: HIF-1α is overexpressed in osteosarcoma and might be closely associated with tumor angiogenesis. Overexpression of HIF-1α might indicate a poor prognosis of osteosarcoma.

Objective To explore the clinical characteristics and diagnosis of mycoplasma pneumoniae(MP) pneumonia showing lobar pneumonia.Methods Patients from Jan.2004 to Dec.2005 were selected as researching case,which had samptoms and signs of respiratory in clinic,the chest Xray shown lobar pneumonia,the MP of down respiratory′s secretion detected by polymerase chain reaction(PCR) and MP-IgM of serum detected by indirect hemagglutination were positive.The clinical data of them were reviewed.Results MP pneumonia showing lobar pneumonia was same to streptococcus pneumoniae pneumonia in clinic,but it had some characters:1.majority of the lobar MP pneumonia were children being student;2.the process was long,the samptoms of respiratory was tipical,the continuous fever was few,and the infectious samptoms was not obvious as streptococcus pneumoniae pneumonia;3.the injury to lung was more and tipical;4.the increase of white blood cell,neutrocyte and C-reactive protein were not clearly as to streptococcus pneumoniae pneumonia,but the absorbing of lung′s focal was relatively fast;5.the ?-lactams antibiotic was not effective,but macrolides was effective.Conclusions Clinical characters of MP pneumonia are needed to master,the variety of chest X-ray changes and differentiate from other pneumonia infected by other are needed to notice.

Objective To investigate the clinical features ,diagnosis and treatment of cesarean scar pregnancy (CSP) .Methods We retrospectively analyzed the medical history ,clinical manifestation ,diagnoses ,treatments and outcomes of 42 patients with ec‐topic pregnancy in the cesarean scar .Results All cases got diagnosed by transvaginal ultrasound .The error rate of first diagnosis was 40 .4% (17/42) .There were 25 cases of conservative therapy ,in which 12 cases were resolved with laparoscopic surgery and 1 case resolved with open surgery ;in the first process 15 cases were carried out laparoscopic surger in which 1 case were converted to laparotomy ;abdominal surgery were underwented in 1 case and were cured .Only one case underwent abdominal total hysterectomy as of failure after uterine artery embolization .After initial conservative treatment 3 cases were hospitalized again and 2 cases under‐went reoperation .41 patients were successfully retained the uterus and normal menstrual cycle returned at two months after opera‐tion .Conclusion The early diagnosis for CSP mainly depended on ultrasound and the thickness of incision muscle layer is an impor‐tant basis for choice of treatment .Checking the thickness of incision muscle layer for CSP mainly depended on ultrasound ,and lapa‐roscopic surgery is an ideal method for diagnosis and treatment of CSP .

Objective: To observe the effect of elk antlers ethanolic fluidextract on behavior and immune function of aging model mice. Methods: ICR mice were randomly divided into 4 groups: Normal control group (NG), model control group (MG), low-dose drug treatment group (LG,2g/kg) and high-dose drug treatment group (HG,4g/kg). Mice were given subcutaneous injection of D-galactose (D-gal) at 120 mg/kg to induce subacute aging model. At the same time LG and HG were respectively administrated corresponding concentration of elk antlers ethanolic extract. Six weeks later, the learning and memory test was run by Y-maze. Then single agar immunodiffusion method was used to detect IgG level in serum, and spleen lymphocyte transforming stimulation index (SI) was determined by methyl thiazolyl tetrazolium(MTT) method as well as the concentration of Interleukin-2(IL-2) and interferon-gama(IFN-?) in serum were determined by ELISA method. Results: The memory and immune function of aging mice declined signif icantly. Elk antlers ethanolic fluidextract could obviously improve memory ability, increase the IgG level, raise the lymphocyte transforming SI, and elevate the concentration of IL-2 and IFN-? in model mice induced by D-gal. Conclusions: Elk antlers ethanolic extract could improve behavior and immune function of aging mice to delay aging process.

Objective To explore the diagnostic and differential diagnostic value of Ki-67,Arg-1,HepPar-1 and Glypican-3 in primary hepatocellular carcinoma and benign hepatocellular lesions.Methods A total of 118 cases of primary hepatocellular carcinomas,100 cases of benign hepatocellular lesions and 263 cases malignant carcinomas originated from other organs were adopted in this study.Immunohistochemistry of Ki-67,Arg-1,HepPar-1 and Gly-3 were performed and evaluated in all specimens.Results The expressions of Ki-67 were concordant with the differentiation of hepatocellular carcinomas and the difference between primary hepatocellular carcinomas and benign hepatocellular lesions were statistically significant (P ＜0.01).The combination of Arg-1 + HepPar-1 + Glypican-3 had the most sensitivity and specificity in differential diagnosis between primary hepatocellular and metastatic malignant cancers,while the combination of Ki-67 + Glypican-3 + Arg-1 showed the better diagnostic value in primary hepatocellular carcinomas and benign lesions.Conclusions The application of different immunohistochemical antibody spectra in primary liver cancers could efficiently improve diagnostic accuracy and reliability.

Objective To investigate the expression of RhoC mRNA in the gastric carcinoma (GC) and paracarcinoma gastric mucosa tissues (PGMT) and their relationship with clinical pathological features.Methods RhoC mRNA was examined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) in 23 cases of primary gastric carcinoma and the paracarcinoma gastric mucosa tissues.Results The opacity density of RhoC mRNA in 23 cases of GC was 1.40±0.23,higher than that of PGMT (0.36±0.15)(P<0.01).In addition.RhoC mRNA in gastric carcinoma tissues were positively related to invasion depth,TNM stage and lymph node metastasis (P<0.01),Conclusion The overexpression of RhoC mRNA in GC may be closely related to the carcinogenesis,development,invasion and metastasis of gastric carcinoma,which is a frefered biomarker for early diagnosis.

Objective To observe the curative effect of low melocular weight heparin(LMWH) on the hypercoagulability in acute Kawasaki disease(KD).Methods Forty-six patients were diagnosed KD.Twenty-two cases out of all KD patients whose serum concentration of whether platelet(PLT) or fibrinogen(FIB) was significantly increased or who were found thrombus in their coronary artery by ultrasonic Doppler were treated with LMWH by subcutaneous injection once every day for 7-10 days.All the patients were divided into 2 groups accor-ding to whether using LMWH or not:H group(using LMWH) and NH group(no using LMWH).It were detected before and after treatment that included thrombin time(TT),activated partial thromboplastin time(APTT),international normalized ratio(INR),FIB,plasma mucosity,erythrocyte sedimentation rate(ESR),hematocrit(HCT) and situation of haemorrhage.Results 1.Before treatment,PLT and FIB of patients in H group were significantly higher than those in NH group(Pa

AIM:To study the effect of different surgeries for vision and postoperative complications in leprosy patients with lagophthalmos.METHODS:A retrospective analysis.Totally 68 leprosy patients (97 eyes) with lagophthalmos were selected who treated with different surgeries during May 2007 to September 2015 in our sanatorium.The surgeries included nylon thread correction surgery, tarsorrhaphy, temporalis transposition surgery.We observed and compared the best corrected visual acuity(BCVA) and the rate of postoperative complications, such as epiphora, lacrimal duct injury, hard to open eyes, infection at the inner and lateral canthus and scar formation of lacrimal apparatus.RESULTS:The effect on BCVA of patients treated with nylon thread correction surgery and tarsorrhaphy:the preoperative and postoperative BCVA was significantly different of the two groups (Z=-4.193,-4.213;P<0.05);the preoperative and postoperative BCVA was not significantly different of the patients underwent temporalis transposition surgery (Z=-1.532, P >0.05).The postoperative complications:(1) epiphora:there was 91% in patients underwent nylon thread correction surgery, 89% in patients underwent tarsorrhaphy, 59% in patients underwent temporalis transposition surgery, the difference was statistically significant (χ2=12.198, P<0.05);(2) lacrimal duct injury:there was 66% in patients underwent nylon thread correction surgery, 95% in patients underwent tarsorrhaphy, 41% in patients underwent temporalis transposition surgery, the difference was statistically significant (χ2=22.415, P<0.05);(3) hard to open eyes:there was 56% in patients underwent nylon thread correction surgery, 55% in patients underwent tarsorrhaphy, 22% in patients underwent temporalis transposition surgery, the difference was statistically significant (χ2=8.795, P<0.05);(4) infections:there was 84% in patients underwent nylon thread correction surgery, 3% in patients underwent tarsorrhaphy, 11% in patients underwent temporalis transposition surgery, the difference was statistically significant (χ2=60.858, P<0.05).CONCLUSION:There are certain clinical efficacy for lagophthalmos caused by leprosy after surgery, especially patients after temporalis transposition surgery can close eyes by oneself.And their vision does not change significantly, which suggests that this surgery may prevent exposure injury for cornea.But the iatrogenic injury, the vision decrease in patients underwent other surgeries and the ocular tissue damage, such as epiphora, lacrimal duct injury, hard to open eyes, infection at the inner and lateral canthus and scar formation of lacrimal apparatus should be pay more attention to.