OBJECTIVE: To prepare solid dispersion of berberine hydrochloride poloxamer 188. METHODS: Melting method was adopted to prepare solid dispersion. The influences of the ratio of the drug to poloxamer 188, melting temperature and cooling temperature on the dissolution of drugs were investigated. The difference of the dissolution between solid dispersion and physical mixture were explored. RESULTS: When the ratio of the drug to poloxamer 188 rose to 1 ∶ 1, the amount of the carrier was enough to distribute the drug evenly. Melting temperature had little influence on the dissolution of drugs while cooling temperature had strong influence on the dissolution of drugs. The fastest dissolution rate was achieved emerged when the cooling tempreture was 0℃. As compared with physical mixture, the dissolution rate of berberine hydroclride was nearly increased one time. CONCLUSION: Berberine hydrocloride poloxamer 188 solid dispersion improves the in vitro dissolution rate of berberine hydrocloride.

Objective To study the clinical features of meniscal cyst of the knee. Methods From January 2001 to December 2007,totally 5986 cases of meniscus operation were performed in our hospital,104 of them were diagnosed as having meniscal cyst in the knee. The clinical data of these patients,including gender and age distributions,causes and mechanism of injury,clinical presentations,characteristics of the cyst,and the type of the injury,were investigated. Results Incidence rate of meniscal cyst was 1.7% (104/5986) and the ratio between medial and lateral meniscal cysts was 1∶12 (8∶96). The mean age of the 104 patients was (30.7?12.2) years (range,13 to 65 years),and the radio of male to female was 1.2 to 1. Of the patients,60 cases (57.7%) had history of trauma,32 or them were hurt in sports,including football (11 cases),basketball (8 cases) and others. The most common primary symptom was pain (102 cases,98.1%),followed by swelling (50 cases,48.1%). Tenderness over the joint line (85 cases,81.7%) and palpable mass (79 cases,76.0%) with or without signs of meniscal tear were common presentations. The anterior and middle portions of the medial meniscus (5/8,62.5%),and posterior extension of the lateral meniscus (88/96,91.7%) were the most commonly involved sites (?2=15.167,P=0.000). The most frequently observed meniscal tear was a horizontal cleavage,alone (25/104,24.0%) or associated with another types of lesions (52/104,50.0%). In 5.8% (6/104) of the cases,the discoid meniscus was involved. Conclusions Meniscus cysts are unusual. The ratio between medial and lateral meniscal cysts favors the lateral meniscus. Meniscal tear and myxoid degeneration are the common causes of the cyst. The most frequently observed meniscal tear is a horizontal cleavage,and the anterior and middle portions of the medial meniscus and posterior extensions of the lateral meniscus are the most commonly involved sites.

Obesity has a harmful impact on health and is imposed by both environmental and genetic factors. Recently, over 60 obesity-related susceptible loci have been identified through genome-wide association study. However, it still remained unknown whether these loci were specific or generalized to the ethnic groups for genetic distinction. This review summarized the obesity-related susceptible loci in Asian populations, which were recently identified among Europeans or Asians.

OBJECTIVE: To investigate the effect of LARS artificial ligament on reconstruction of anterior and posterior cruciate ligament of keen joint.METHODS: Articles addressing reconstruction of anterior and posterior cruciate ligament by LARS artificial ligament were retrieved from VIP database (http://www.cqvip.com/) with the key words of "LARS artificial ligament, cruciate ligament,reconstruction" in Chinese between January 2000 and February 2010.Inclusion criteria: advantages and disadvantages of reconstructing anterior and posterior cruciate ligament by LARS artificial ligament; clinical application of reconstructing anterior and posterior cruciate ligament by LARS artificial ligament.Exclusion criteria: Duplicated studies or old references were excluded.A total of 31 articles were included in the final analysis.RESULTS: Reconstruction of anterior and posterior cruciate ligament by LARS artificial ligament characterized by convenient operation, short operative time, mild trauma, engaging in movement early, great strength, simple implantation, and reliable fixation; however, most patients were not able to bear the high medical cost, thus clinical application was limited.Scholars thought that LARS artificial ligament had great effects on reconstruction of anterior and posterior cruciate ligament.CONCLUSION: Patients who had multi-ligament injury, overhauling of ligament, and early movement were treated with LARS artificial ligament.

With the enforcement of YY 0505-2012 standard,more attention has been paid to the electromagnetic compatibility test of medical electrical equipment.In all the test items involved in YY 0505-2012,the radiation emission test is the most complicated and the hardest to be passed,so it is very important to master the test method.Microwave therapy equipment is a kind of equipment using microwave radiation energy to treat diseases,and is supervised as class Ⅲ medical equipment.According to the requirements of the relevant standards,all the electromagnetic compatibility testing items of microwave equipment are listed,and the test difficulty and the test focus of the equipment are studied.In the frequency band of the radiation emission experiment,microwave treatment equipment is divided into different categories according to the purpose and environment of the application.By comparing the data of the tests,the differences of different classes of microwave treatment equipment in the radiation emission tests are summarized.

Objective To observe the effect and mechanism of quercetin on the proliferation of rat hepatic stellate cell(HSC)induced by TGF-β1.Methods HSC was isolated by orthotopic hepatic perfusion with collagenase Ⅳ.MTT assay was used to detect the inhibitory effect of quercetin on the proliferation of HSC.The expression of typeⅠcollagen in HSC was detected by ELISA.The effect of quercetin on the expression of typeⅠcollagen,Smad2 and Smad7 mRNA in HSC was detected by real-time quantitative PCR.The expression of type Ⅰ collagen,Smad2 and Smad7 protein in HSC was detected by Western blot.Results Quercetin could inhibit the proliferation of HSC,down-regulated the expression of type Ⅰ collagen,Smad2 mRNA and protein,and Smad7 mRNA and protein expression were significantly up-regulated(all P<0.05).Conclusion The anti-hepatic fibrosis effect of quercetin may be related to its blocking TGF-β1/Smad pathway of HSC and blocking the activation and proliferation of HSC.

Tumor metastasis is a primary cause of leading to death of tumor patients,and difficult to control.The relationship between primary tumor(PT)and metastasis tumor(MT)is extremely complicated. Considerable animal experiments and clinical data confirm that treatment of PT encourages the growth of MT, but the mechanism is not still fully clear.Some studies show that the density depression of antiangiogenesis factor and immune escape of tumor cell are main reasons for MT accelerated growth.This article reviews the animal experiment,clinical observation and occurrence mechanism of primary tumor treatment promoting tu- mor metastasis.

Community associated methicillin-resistant Staphylococcus aureus is a human pathogen.It can cause a series of infections cause morbidity and mortality,including bacteremia,pneumonia and soft tissue infections.USA300 clone is highly toxic and contagious.Its prevalence in the United States continues to rise,and has begun to spread to the rest of the world.This article briefly reviews the recent research on relevant aspects of molecular epidemiological characteristics,grug resistance mechanisms and treatment of USA300 clone.

Objective To observe the value of drug therapy by the arterio of thigh in the treatment of osteosarcoma of the leg bones. Methods 13 cases of patients with osteosarcoma of the leg bones were treaded with DDP, ADM and VCR by the arterio of thigh, IFO by phlebo. Results The response rates(PP+CR) to osteosarcoma lesions were 69.2 %. Conclusion The comdination regimen with drug therapy by the arterio of thigh is simple and safe and effective for osteosarcoma of the leg bones, which is worth spreading and applying.

By establishing a series of Dynamic Cervical Implants (DCI) within C5-C6 cervical spinal segments, the biomechanical finite element analysis for DCI with different width and thickness were carried out to investigate the influence of the width and thickness of DCI's curved section on its equivalent stress and range of motion (ROM), so as to provide some theoretical basis for the optimization of DCI's design. The results show that the width of DCI's curved section has more obvious influence on the ROM of lateral bending and torsion, in comparison with the thickness of DCI's curved section. By appropriate reduction in width, the ROMs of lateral bending and torsion increase obviously, i.e. the overall movement function of patients is improved. Furthermore, the increase of equivalent stress could be counteracted by corresponding increase of thickness.
Biomechanical Phenomena ; Cervical Vertebrae ; Finite Element Analysis ; Humans ; Movement ; Neck ; Prostheses and Implants ; Prosthesis Design ; Range of Motion, Articular