Objective To evaluate the effect of cold ischemia on the expression of tumor necrosis factor alpha (TNF-?) and interleukin-10 (IL-10) and hepatic regeneration in liver grafts.Methods Partial liver transplantation model in rats was developed. The rats were divided into 3 groups: control group receiving 50 % liver resection,experimental group 1 receiving partial liver transplantation 30 min after cold ischemia and experimental group 2 receiving partial liver transplantation 10 hr after cold ischemia. The expression of the proliferative cell nuclear antigen (PCNA),TNF-? and IL-10 in liver tissues obtained on the postoperative day 1,2 and 4 in rats was detected by using immunohistochemical method. Correlation analysis was performed on the liver proliferation and regeneration,and the expression of TNF-? and IL-10.Results The survival rate in control group,experimental group 1 and experimental group 2 was 100 % , 79 % and 29 % respectively. In the experimental group 2,the expression of PCNA,TNF-? was significantly decreased,while the expression of IL-10 increased as compared with that in experimental group 1 (both P

Objective To summarize experience of selection of donors of living related liver transplantation (LRLT) for Wilson’s Disease.Methods Twenty-nine cases of Wilson’s disease were subjected to LRLT in our center. A retrospective data analysis was carried on 70 potential donors. Results Twenty-three of the 70 potential donors were excluded. The causes were as follows: family and traditional background (n=10, 43.5 % ), copper metabolism abnormality, serum ceruloplasmin 100 ?g (n=6, 26.1 % ), hepatic steatosis (n=4, 17.4 % ) and hepatitis B (n=3, 13 %). The grafts were right liver (n=10) and left liver (n=19). Graft-recipient weight ratio was ( 1.38 ? 0.44 ) %. All donors recovered completely. Perioperative surgical complications occurred in 3 donors and were cured without reoperation, temporary biliary leakages (n=2) as well as postoperative bleeding from the wound of liver (n=1). Conclusions Major impact on living relative donors for Wilson’s Disease is traditional background, copper metabolism abnormalities, hepatitis B and hepatic steatosis. Comprehensive preoperative evaluation of potential donors and harvesting liver surgery innovation ensure the donor safety and graft quality.

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Neural Conduction ; physiology ; Paraplegia ; diagnosis ; physiopathology ; Sural Nerve ; physiopathology

Objective To explore the relation between thyroid nodular and thyroid calcification .Methods The clinical data of 430 cases with nodular goiter admitted from January 2011 to December 2013 were reviewed.All cases were performed ultrasonography and pathologic examination .Results Of 430 cases,there was thyroid carcinoma in 49 cases(11.4%),including microcalcification in 30 cases(61.2%) and macrocalcification in 3 cases(6.1%);benign lesion in 381 cases(88.6%),including microcalcification in 24 cases(6.3%) and macrocalcification in 39 cases(10.2%).The difference was remarkable (P=0.000).There was remarkable difference in malignant calci-fied nodules ratio between the patients with microcalcification and macrocalcification (55.6%vs 7.1%,P=0.000), between the patients aged 45 years below and above(48.8%vs 22.6%,P=0.007),between the patients with solita-ry and multiple nodules(45.0%vs 16.7%,P=0.005).But there was no statistic difference in malignant calcified nodules ratio between the male and female groups (24.2% vs.39.7%,P=0.130).Conclusion Microcalcification is a highly specific sign to predict thyroid carcinoma in ultrasonography exam .Positive intervention will be performed immediately when calcification appears in thyroid nodules .

Objective To investigate the feasibility of qualitative diagnosis of small pulmonary nodules (≤1 cm) using multi‐plane reconstruction (MPR) and volume rendering (VR) techniques based on spiral CT scan .Methods The CT images of 190 patients with small pulmonary nodules (≤1 cm) were analyzed retrospectively ,and compared the detection rates of several imaging features among different pathological types of nodules .Results In these 190 patients who were diagnosed as malignance by CT ,168 were confirmed by pathology with an accuracy rate of 88 .4% .The imaging features such as ground glass nodule ,vascular convergence in the benign group were significantly lower than those in the malignant group ,however the solid nodule was observed more frequently in the malignant group (P<0 .05) .The significant differences in other imaging features were not found (P>0 .05) .Except air bronchogram and vascular convergence ,some imaging features (pure ground glass nodule ,part solid ground glass nodule ,solid nodule) showed a decreasing trend ,whereas some others (speculation ,lobulation ,vacuole sign and pleural tag ) were increased among different pathological types of malignant nodules .The linear trend passed the significant test at 0 .017 level .Conclusion CT multi‐plane reconstruction (MPR) and volume rendering (VR) techniques can sufficiently demonstrate the malignant signs in small pulmonary nodules (≤1 cm ) ,can improve to identify different pathological types of such small pulmonary lesions .

Adult ; Asthma ; metabolism ; physiopathology ; Basic-Leucine Zipper Transcription Factors ; genetics ; physiology ; Fanconi Anemia Complementation Group Proteins ; genetics ; physiology ; Female ; Glutamate-Cysteine Ligase ; metabolism ; Humans ; Inflammation ; metabolism ; pathology ; Male ; NF-E2-Related Factor 2 ; genetics ; physiology ; RNA, Messenger ; genetics ; metabolism ; Sputum ; cytology

Objective To investigate the therapeutic and preventive method of foreignbodies in esophagus.Methods Clinical date of 78 cases of foreignbodies in esophagus were analyzed retrospectively.Results Among the 78 foreignbodies in esophagus,76 cases were removed from esophagus in different way.2 cases fulled into mastach.No severe complication occurred.The cure rate was 97％.Conclusions Foreignbodies in esophagus should be removed as soon as it is diagnosed.It is important that correctly treating foreignbodies in esophagus can reduce the incidence of severe complications.

Objective To explore the value of follow-up of serum TSH stimulating thyroglobulin (Tg) test and 131I whole body scan(WBS) in patients with differentiated thyroid cancer(DTC) who were treated by 131I.Methods By SPSS software,Kappa test was performed between results of serum Tg,TgAb test and WBS for diagnosis of persistent/recurrent DTC.Results Consistency test of TSH stimulating Tg,TgAb and 131I WBS for diagnostic persistent/recurrent or metastasis of DTC:kappa value =0.587,SE =0.076,P ＜ 0.01.Although the results showed that two measurements appeared definite consistency,it is dissatisfactory.Conclusion Serum TSH stimulating Tg test and 131I whole body scan(WBS) are important follow-up tools for patients with DTC.Because of Tg interfering it is necessary to performed TgAb test simultaneously,when Tg test was performed.Especially 131I WBS do is not absent because false negative would be able to appeared in single serum TSH stimulating Tg and TgAb test.

Objective To establish a new method to analyze the position accuracy of multileaf collimator (MLC) in the dynamic mode.Methods The MLC test sequence was created in a field,where intentional leaf positional errors ranging from 0.1 to 1 mm per centimeter were introduced.In order to establish the relationship between the ion chamber readings and leaf position,whose slope indicated the leaf position error per centimeter,a two-dimensional ion chamber array was used to measure absorbed dose while leaves were moving at dose rates of 100,300 and 600 MU/min,respectively.For routine test,leaf position error was easily found via dose profile in y direction of the field created by dynamic leaves,where the position error could be quantitatively calculated as the slope of absorbed dose line of x direction of the same field.Results The error of 0.2 mm or more per centimeter was obviously shown through y dose profile.The calibration curve was linear at different dose rates.At 600 MU/min,a 0.1 mm leaf position error corresponded to a slope variation of 0.74％,and the differences between the tested errors and the introduced errors were within 0.1 mm.Conclusions The simple and reliable method is helpful to establish the intensity modulated radiation therapy (IMRT) quality control (QC) system.

Objective To investigate the diagnostic value of T-SPOT.TB in patients with fever.Methods T-SPOT.TB was carried out in 456 febrile patients who had no determined reason for fever or who could not be excluded from infection with Mycobacterium tuberculosis.These patients were followed up for their final clinical diagnosis and the diagnostic value of T-SPOT.TB was evaluated using the receiver operating characteristic (ROC) curve.Results In the present study,85 patients were clinically diagnosed with tuberculosis (TB) (including 34 cases of pulmonary TB,24 cases of TB infection defined as T-SPOT.TB positive without TB lesion,but effective with anti-TB treatment,10 cases of tuberculous pleurisy and 17 cases of other extrapulmonary TB) and 371 patients had no definite evidence of Mycobacterium tuberculosis infection (including 42 cases of suspected TB and 329 cases of other diseases).T-SPOT.TB had a high sensitivity of 84.3％ in TB patients and a high negative predictive value of 94.2 ％,but the specificity was 68.1％ and the positive predictive value was 41.4 ％.The Kappa value was 0.380 and the area under ROC curve was 0.762 (95％CI:0.707-0.817).T-SPOT.TB had a higher sensitivity in extrapulmonary TB than in pulmonary TB (88.2 ％ vs 78.1 ％),with the negative predictive values of 95.3 ％ and 92.6 ％,Kappa values of 0.438 and 0.299,and the areas under ROC of 0.791 (95 ％ CI:0.724-0.857) and 0.719 (95％CI:0.623-0.815),respectively.Ninety-nine cases were T-SPOT.TB positive out of 329 patients diagnosed with other diseases.Conclusions T-SPOT.TB test can be used as a method for TB diagnosis in patients without obvious TB lesions.Due to its relatively high negative predictive value,T-SPOT.TB can be used to rule out infection with Mycobacterium tuberculosis.