Objective:To probe the correlation of intra-operative hyaluronic acid(HA) changes with hepatic allografts cold ischemia reperfusion injury in liver transplantation.Methods:The patients with orthotopic liver transplantation were assigned to 3 groups:① group A :liver allografts were stored in University of Wisconsin's solution(0℃) for 5-6 h before implantation;② group B :liver allografts were stored in U W's solution(0℃) for 8-9 h before implantation;③ group C :liver allografts were stored in U W's solution(0℃) for 12 h before implantation.The serum samples were taken up at 5 min,60 min,120 min,180 min after the donor liver was transplanted and reperfused to detect the concentration of HA.Results:After donor liver was transplanted and reperfused,serum HA concentration decreased immediately and gradually.HA decreased rate :group A 4.58 ?g/L?min-1,group B 4.16 ?g/L?min-1,group C 2.26 ?g/L?min-1.Serum HA decrease rate was faster in group A and B than that in group C(P

Highly active antiretroviral therapy (HAART) can effectively suppress thehuman immunodeficiency virus (HIV) replication andsignificantly reduce morbidity and mortality of HIV-infectedpatients,which howevercan't completely remove the virus,and eventually progressinto chronic viral-infection disease.Chronic HIVinfection destroys host immune system,leading to intestinal barrier damage,intestinal mucosal dysfunction,microbial translocation,and further accelerates the disease progress.The reconstruction of intestinalmicroflora balance and improvement of intestinal mucosa function areessential to reestablish the host immune system.This paper will review the current research advanceson intestinal barrier damage of HIV infection and gut-target therapy of AIDS.The aim is to provide valid evidences for further research targeting improvement of treatment strategiesandreduction of morbidity and mortality in HIV infection.

Biopsy ; methods ; Drainage ; Humans ; Jaundice, Obstructive ; etiology ; therapy ; Lymph Node Excision ; methods ; Neoplasm Staging ; Pancreatic Neoplasms ; complications ; diagnosis ; pathology ; surgery ; Pancreaticoduodenectomy ; methods ; Salvage Therapy

Objective To evaluate the effect of cold ischemia on the expression of tumor necrosis factor alpha (TNF-?) and interleukin-10 (IL-10) and hepatic regeneration in liver grafts.Methods Partial liver transplantation model in rats was developed. The rats were divided into 3 groups: control group receiving 50 % liver resection,experimental group 1 receiving partial liver transplantation 30 min after cold ischemia and experimental group 2 receiving partial liver transplantation 10 hr after cold ischemia. The expression of the proliferative cell nuclear antigen (PCNA),TNF-? and IL-10 in liver tissues obtained on the postoperative day 1,2 and 4 in rats was detected by using immunohistochemical method. Correlation analysis was performed on the liver proliferation and regeneration,and the expression of TNF-? and IL-10.Results The survival rate in control group,experimental group 1 and experimental group 2 was 100 % , 79 % and 29 % respectively. In the experimental group 2,the expression of PCNA,TNF-? was significantly decreased,while the expression of IL-10 increased as compared with that in experimental group 1 (both P

Objective To summarize experience of selection of donors of living related liver transplantation (LRLT) for Wilson’s Disease.Methods Twenty-nine cases of Wilson’s disease were subjected to LRLT in our center. A retrospective data analysis was carried on 70 potential donors. Results Twenty-three of the 70 potential donors were excluded. The causes were as follows: family and traditional background (n=10, 43.5 % ), copper metabolism abnormality, serum ceruloplasmin 100 ?g (n=6, 26.1 % ), hepatic steatosis (n=4, 17.4 % ) and hepatitis B (n=3, 13 %). The grafts were right liver (n=10) and left liver (n=19). Graft-recipient weight ratio was ( 1.38 ? 0.44 ) %. All donors recovered completely. Perioperative surgical complications occurred in 3 donors and were cured without reoperation, temporary biliary leakages (n=2) as well as postoperative bleeding from the wound of liver (n=1). Conclusions Major impact on living relative donors for Wilson’s Disease is traditional background, copper metabolism abnormalities, hepatitis B and hepatic steatosis. Comprehensive preoperative evaluation of potential donors and harvesting liver surgery innovation ensure the donor safety and graft quality.

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Neural Conduction ; physiology ; Paraplegia ; diagnosis ; physiopathology ; Sural Nerve ; physiopathology

Protein Kinase C (PKC) is an important messenger in intracellular signal transduction. So far, at least 11 members of PKC isoforms have been isolated and purified. The mutation of the non-synonymous SNP (1425G/A) of the η isoform of protein kinase C (PKC η), a protein kinase Cη gene (PRKCH) may result in the increased PKCη activity, which is considered as a new risk factor for lacunar infarction. In recent years, the studies about the role of PRKCH in cell differentiation and apoptosis and its relation with some signal transduction pathways have made some new advances, especially, PKCη participates in the regulation of some key enzyme activity that mitogen-activated protein kinase, inducible nitric oxide-synthase and matrix metalloproteinase are closely correlated with the process of atherosclerosis. It will provide a new way of thinking for the clinical intervention of cerebral infarction in the future.

Nerve agents (NAs) belong to the class of organic phosphorus compounds which are acetylcholinesterase ( AChE) inhibitors, including soman, sarin, tabun,VX, etc.NAs are extremely toxic and considered as the most danger-ous chemical warfare agents.The current standard treatment for poisoning by nerve agents consists of the combined adminis-tration of anticholinergic drugs such as atropine sulphate, AChE reactivators such as pralidoxime, obidoxime and HI-6 and diazepam for anticonvulsant effects, but oximes are therapeutic antidotes against nerve agent intoxications which exert the therapeutic purposes primarily by reactivating the NAs-inhibited AChE.In this paper, the mechanism of nerve agents, the main working procedure of anti-NAs drugs, the chemical structure of classic reactivator, the corresponding antitoxic action, in vivo and in vitro effects and metabolic kinetics are reviewed.

Objective To explore the improvement of traditional incision in thoracotomy and the application of subaxillary vertical small incision in lung operations. Methods We carried out a retrospective analysis on documents of 680 cases of subaxillary vertical small incision pneumonectomy conducted in this hospital between December 1996 and June 2003. Results The length of incision was 8~13 cm (mean, 11 2 cm). The time for thoracotomy was 4 5~10 min (mean, 6 min) and the operation time was 50~ 170 min (mean, 135 min). The intraoperative blood loss was 100~1200 ml (mean, 350 ml), the postoperative drainage volume was 120~800 ml (mean, 300 ml), and the perioperative blood transfusion, 0~1400 ml (mean, 300 ml). Postoperative pain classification results revealed that 585 cases of grade 1, 60 cases of grade 2 and 35 cases of grade 3 severity were observed. The postoperative hospital stay was 10~21 days (mean, 14 days). No surgical death occurred. Conclusions Subaxillary vertical small incision can be simply made. It offers minimal invasion, less blood loss, mild postoperative pain, quick recovery and good cosmetic results, being a viable option in most operations of pneumonectomy.

Objective To observe the clinical effects of chemotherapy with intraperitoneal hyper-thermal perfusion for malignant ascites. Methods 40 patients with malignant ascites were randomly divided into two groups. In the treated group, 21 patients underwent intraperitoneal hyperthermal perfusion and local thermotherapy. 19 patients in the control group received commonly intraperitoneal perfusion. Results The CR, PR patients were 6,10 and 0,7 in the treated group and control group, respectively. The efficacy rate in the treated group was significantly higher than that in the control group. 2 patients oc-cured bellyache in the treated group and 1 patient in the control group, there was no statistically significant difference between the two groups. Conclusion Chemotherapy with intraperitoneal hyperthermal perfusion can significantly improve the therapeutic effects for malignant ascites, and has no more side effects.