The effects of MC903, a novel 1,25-dihydroxyvitamin D3 [1,25(OH)2 D3] analogue, on proliferation and differentiation of a human megakaryoblastic leukemia cell line (HIMeg) were investigated. MC903 was found to inhibit cell proliferation and induce cell differentiation of HIMeg cells. Colony formation assay showed that MC903 (10-10 to 10-6mol/L) almost completely inhibited the colony-forming ability of HIMeg cells. Cell cycle analysis demonstrated that MC903 altered the cell cycle distribution; the fraction of cells in G0 + G1 increased while those in S and G2 + M decreased after treatment with MC903 for 4d. The effect of MC903 on proliferation and differentiation of HIMeg cells was in a dose- and time-dependent manner and was more active than that of 1, 25(OH)2, D3,. The results of the present study suggested that 1,25(OH)2 D3 as well as its novel analogue MC903 might play an important role in modulating the proliferation and differentiation of megakaryocytic lineage.

The cellular effects of glucocorticoids on proliferation and differentiation of a human inegakaryoblastic leukemia cell line (HIMeg) were studied in comparison with sex steroid hormones. In both liquid and methylcellulose culture systems, glucocorticoids suppressed the proliferate of HIMeg cells in a dose-dependent manner. In contrast, sex steroid hormones except progesterone had little effects on the proliferation of HIMeg cells. In liquid culture systems, morphological changes were not evident, and the percentage of cells with multilobular nuclei increased slightly from 2% to 8% after glucocorticoid treatment. Similarly, only 2% of HIMeg cells expressed glycoprotein Ⅱb/Ⅲa (GP Ⅱb/Ⅲa) antigen without hormone, whereas 30% of the cells expressed GP Ⅱb/Ⅲa with the addition of glucocorticoids. These data indicated that glucocorticoids could induce differentiation of HIMeg cells. To clarify the molecular mechanisms, glucocorticoid receptor (GR) expression was examind by Scatchard analysis, and it was found that there was a saturable, high affinity GR in HIMeg cells. Furthermore, the cellular effects of glucocorticoids on HIMeg cells could be reversed by RU486, a potent glucocorticoid antagonist. These observations suggest that the cellular effects of glucocorticoids on HIMeg cells were mediated by GR.

Objective To summarize the experience on bronchiallavage to cure pulmonary atelectasis and pulmonary infection after thoracotomy.Methods 48 patients were selected and treated by bronchiallavage under monitoring,and antibiotics was used according to sputum culture and drug sensitive test.The out come was analyzed and evaluated.Results All patients of the pulmonary atelectasis were cured and those with pulmonary infection were controled.There was no serious complication.Conclusion Bronchiallavage is applied to treat pulmonary atelectasis and pulmonary infection after thoracotomy,the curative rate is significant.The appliance of antibiotics is guided by drug sensitivity.It is such a safe and feasible therapeutic tool that the bronchofibroscope is applied to cure pulmonary atelectasis and pulmonary infection after thoracotomy.

Objective To investigate the chemical constituents in the leaves of Dracaena cochinchinensis.Methods The compounds were separated with column chromatography and their chemical structures were identified by physicochemical and spectral method,respectively.Results Thirteen compounds were isolated from the plant. They were identified as isorhamnerin(Ⅰ),quercetin(Ⅱ),25(R)-spirostane-5-en-3?-ol(Ⅲ),gracillin(Ⅳ),25(R)-spirostane-5-en-3?,14?-diol(Ⅴ),25(R)-spirostane-5-en-3?,14?-diol-3-O-?-D-glucopyranoside(Ⅵ),25(R)-spirostane-5-en-3?,14?-diol-3-O-?-L-rhamnopyranosy(1→4)-?-D-glucopyranoside(Ⅶ),25(R)-spirostane-14?-hydroxy-4-en-3-one(Ⅷ),7?-hydroxysistosterol-3-O-?-D-glucopyranoside(Ⅸ),?-stigmasterol(Ⅹ),stigmasterol-3-O-?-D-glucopyranoside(Ⅺ),daucosterol (Ⅹ Ⅱ),and methyl ?D-glucopyranoside(Ⅹ Ⅲ).Conclusion Spirostane-type steroids are the major constituents in the leaves of D.cochinchinensis.Compounds Ⅰ-Ⅲ,Ⅴ,Ⅵ,Ⅷ,and Ⅸ are isolated from this plant for the first time.

The activity of the negative regulator of cell cycle, P27, affects the normal process of cell cycle. As a type of cell cycle diseases, tumor is closely correlated with the activation of P27. It is shown that the level of P27 decreases in most kinds of tumors.By its influence on cell proliferation and apoptosis, P27 plays an important role in the occurrence and development of tumor. The activity of P27 is regulated by several pathways either at or after transcription, so various ideals which directly change the protein level of P27 or indirectly interfere the activities of elements in regulative pathways of P27 will provide many novel and efficient ways for therapies in tumors.

Thirty strains of seven Candida species from CICC(China Center of Industrial Culture Collection)were studied. The strains were differentiated by ITS1 region PCR-SSCP fingerprinting analysis and ITS2 region PCR-SSCP fingerprinting analysis. Results showed that both ITS1 region and ITS2 region were able to differentiate the seven species of Candida clearly. Contrasting the maps and effects on the identification of Candida species of ITS1 region with that of ITS2 region, result indicated that on the identification of Candida species the application of ITS2 region was better than ITS1 region.

The author reviewed some researches on teacher's work related stress in China and abroad. The present researches mainly focus on the sources of stress in teachers' work life. Some practices of work related stress in USA and Chinese special education field were discussed in the article. In the end, the author took a perspective on future research of teacher's work related stress in Chinese special education field.

Objective To observe the effect of rosuvastatin and memantine on EPC and learning or memory ability in VaD rats .Methods Forty adult male SD rats were randomly divided into sham operation group ,VaD model group ,memantine treatment group ,rosuvastatin treatment group , and memantine+rosuvastatin treatment group (combined treatment group) (8 in each group) .A VaD model was established by permanent ligation of bilateral common carotid arteries .The ani‐mals in rosuvastatin treatment group ,memantine treatment group and combined treatment group received gastric rosuvastatin 10 mg/(kg · d) ,memantine 10 mg/(kg · d) and memantine 10 mg/(kg · d)+rosuvastatin 10 mg/(kg · d) for 4 weeks while those in model group received gastric normal saline .Five weeks after operation ,the learning or memory ability was tested by Morris water maze test ,the escape latency and percentage of target quadrant were calculated ,the circulat‐ing EPC were detected by flow cytometry ,and the MVD in hippocampaus was assyed with vWF immunostaining .Results Five weeks after operation ,the learning or memory ability was signifi‐cantly lower in model group than in sham operation group (P< 0 .01) whereas the learning or memory ability ,the percentage of circulating EPC and the MVD in hippocampus were significantly higher in 3 treatment groups than in model group (P<0 .05) .Conclusion Rosuvastatin and me‐mantine can effectively improve the learning or memory ability of VaD rats by mobilizing their cir‐culating EPC and increasing the MVD in their hippocampus .However ,the effect of memantine or rosuvastatin does not differ greatly w hen they are used alone .

Objective To investigate the training of recruits′psychological stress and its relevant influencing factors ,so as to of‐fer scientific basis for the psychological training and psychological health education of recruits .Methods Questionnaire survey method was used and a total of 720 training of recruits in 2013 was sampled .The application of the stress response questionnaire (SRQ) ,eysenck personality questionnaire (EPQ) ,trait coping style questionnaire (TCSQ) ,symptom checklist (SCL‐90) were used to carry out the psychological test and analysis on these recruits .Results The total score of the SCL‐90 and the score of each factor were significantly lower than the norm of recruits in year of 2000(P<0 .01) .Compared with the norm of SCL‐90 of Chinese sol‐diers in 2004 ,the factor score of somatization ,anxiety and terror were significantly higher than those of domestic soldiers .However , the factor score of hostility and bigotry were significantly lower than those of domestic soldiers (P<0 .05) .The new soldiers who were the only child of the family and was born with urban registered permanent residence had more obvious psychological stress symptoms .The total score of psychological stress and each factor score of new soldiers with different educational level had no sig‐nificant difference (P>0 .05) .According to what was shown in the related analysis ,the SCL‐90 total score .SRQ total score had significant positive correlation with emotional stability and negative coping style(P<0 .01) ,but had significant negative correlation with introversion‐extroversion and waste score(P<0 .01) ,and the total score of SCL‐90 had significant positive correlation SRQ to‐tal score(P<0 .01) .It was shown in the regression analysis that factors had significant predictive effect on the psychological factors of new soldiers during the training period respectively were as follow :negative coping ,stability ,introversion‐extroversion ,psychoti‐cism and whether they are the only child or not .The decisive coefficient of 5 variables was R2 =0 .558 ,F=163 .65(P<0 .01) .Con‐clusion The overall mental health of 2013 recruits training are in good condition ,individual mental health status ,personality traits and coping style training of recruits psychological stress are significant predictors .

Objective To study the protective effect and Bcl-2 expression of salvia miltiorrhiza pretreatment on retinal ischemia-reperfu-sion injury ( RIRI) . Methods One hundred and thirty two Wistar rats were randomly divided into the normal control group, the ischemia-reperfusion group and the salvia miltiorrhiza pretreatment group. The model of retinal ischemia-reperfusion injury was constructed by increas-ing the intraocular pressure. The ischemia-reperfusion and salvia miltiorrhiza pretreatment group were divided into five subgroups according to the different reperfusion time (6 h, 12 h, 24 h, 48 h and 72 h). Observe the histological changes in retina by HE staining. The SABC ( strept avidin-biotin complex) and Western-blot were used to measure changes of Bcl-2 protein levels in retinal. Results The positive ex-pression of Bcl-2 protein was weak in normal group. In the ischemia-reperfusion group and salvia miltiorrhiza pretreatment group, the expres-sion of Bcl-2 protein began to increase at 6 hours after reperfusion, reached the peak at 24 hours after reperfusion, began to decrease at 48 hours after reperfusion, and started to weaken at 72 hours after reperfusion. The variation tendency of the two groups were the same, however, the expression of Bcl-2 was significantly stronger in the salvia miltiorrhiza pretreatment group compared with ischemia-reperfusion group, and there was significant difference between the two groups (P<0. 01). Conclusion Salvia miltiorrhiza pretreatment can protect the retina by reducing retinal ganglion cells apoptosis in retinal ischemia-reperfusion injury. The mechanism may be achieved by regulating the expression of Bcl-2 protein.