Multi-drug resistance (MDR) of cancer cells is a major cause of failure of chemotherapy in cancer treatment. Most MDRs are caused by overexpression of P-glycoprotein (P-gp) which effuses chemotherapeutic agents out of cells. Nano drug delivery systems (NDDS) have been valued for their characteristics including drug targeting, slow-releasing, and long circulation time in the body. Through mechanisms such as non-specific endocytosis and receptor-mediated endocytosis, NDDS can inhibit P-gp at the gene and protein level, leading to improved concentrations of chemotherapeutic drugs in cancer cells, and therefore reverse MDR.

Objective Objective To study the application of oral ranitidine to MRCP. Methods 20 healthy subjects, including 12 males and 8 femals, were performed with random double-blind MRCP examination by 1.5T MR machine. Two experienced radiologist assessed the results. Results Without any side effect, the proximal end and distal end of the common bile duct, bile-cyst were displayed well (P

To discuss the immune mechanism of the acupuncture and moxibustion on allergic rhinitis(AR),which resulted from the summary of the immune mechanism research on AR in the last more than ten years,so as to improve diagnosis and treatment of the acupuncture and moxibustion on AR.To analyze and summarize the immune mechanism of the acupuncture and moxibustion on AR,which based on the data about that in the last more than ten years.

AIM　to compare the bioavailability and pharmacokinetics of two acyclovir tablets. METHODS　Concentrations of acyclovir in 10 men serum after po 600 mg of two acyclovir tablets were determined , in random 2-way crossover design. Pharmacokinetic parameters were also estimated. RESULTS　The peak concentrations of test and reference tablets were 1.24±0.49 and 1.17±0.20 μg/ml, the AUC 7.56±1.80 and 7.56±2.10μg*h/ml, respectively. The relative bioavailability was 101.36%±11.62%. The test formulation was found bioequivalent to the reference in AUC and Cmax by two one-side t test. CONCLUSION　Two tablets were bioequivalent.

Objective To investigate the employment pressure among graduates from nursing school,and explore influencing factors,in order to make suggestions on education management,career guidance and psychological intervention.Methods Students graduated from Nanyang Medical College in 2012 who majored in nursing and nursing graduates from Nanyang Institute of Technology were surveyed with medical students employment pressure questionnaire designed by DU Tian-jiao from China Medical University.SPSS13.0 was used to establish database and analyze data.Results The total employment pressure of all students was M=3.2.Seven stressors scores from high to level were:post requirements,social environment,family support,personal qualities,the school effect,professional requirements,and career guidance.Multiple regression analysis showed that whether or not the student leaders,different sources and the education background were the influencing factors of employment pressure.Conclusions This research suggests that different psychological intervention should be given to students majored in different specialty and sources in order to release employment pressure and anxiety.

Objective To observe the effects of camellia oil on the development of allergic contact dermatitis in mice. Methods Mice models of allergic contact dermatitis were established with dinitrofluo-robene (DNFB). Then, certain concentrations of camellia oil and halcinonide were used topically on the ears of mouse models twice daily for 6 days. Skin specimens were obtained from the treated ears and subjected to histology examination and pathological analysis. RT-PCR was performed to detect the mRNA expressions of interleukin 2 (IL-2) and interferon γ (IFN-γ) in the skin tissues. Results Allergic contact dermatitis was successfully induced by DNFB in mice with a marked increase in the mRNA expression of IL-2 and IFN-7. There was a slight decrease in the ear swelling degree, inflammatory reaction intensity, number of infiltrating inflammatory cells, and pathological severity in camellia oil-treated mice and halcinonide-treated mice compared with glycerinetreated mice. Camellia oil and halcinonide also induced different degrees of reduction in the mRNA expression of IL-2 and IFN-γ in inflammatory tissues. Conclusion Camellia oil can alleviate the pathological damage and down-regulate the expression of IL-2 and IFN-7 in mice with DNFB-induced allergic contact dermatitis.

Objective To study the clinical characteristics, the outcome of pregnancy and the risk factors of uteroplacental apoplexy complicating severe placental abruption. Methods A retropectively study of the 52 cases of placental abruption who had delivered in our hospital from Jan. 2002 to Dec. 2006 was conducted. These cases were divided into 2 groups: 17 cases of uteroplacental apoplexy complicating placental abruption as observation group, the others with no uteroplacental apoplexy as control group. The risk factors of disease, clinical characteristics and the outcome of pregnancy between the two groups were compared. Results (1)The incidence of placental abruption was 0. 15% (52/35 049) among the total deliveries patients with uteroplacental apoplexy complicating placental abruption took up 0. 05% (17/35 049) of all deliveries and 33% (17/52) of all abruption cases. (2) General information and delivery : There were no significant differences ( P > 0. 05 ) regarding their mean age and BMI in two groups. All women in observation group had C-section delivery, which were 21 in control group. 14 women had vaginal delivery. The incidence of premature labour was 88% ( 15/17 ) in observation group, and 49% (17/35 ) women in control group delivered after 37 weeks. Significant differences were observed regarding delivery methods and gestational weeks(P <0. 01 ). (3)Risk factors: the incidence of preeclampsia, 71% (12/17), and the duration of disease, 6. 4 hours, in observation group were more than those in control group, 20% (7/35) and 4. 2 hours( P < 0.01 ). There were no significant differences between two groups in premature rupture, polyhydroamnions ( P > 0. 05 ). (4) Clinical characteristics in two groups : bloody amniotic fluid, fetal distress, hematometra and postpartum hemorrhage occurred in 82% (14/17) vs 26% (9/35), 65%(11/17) vs 29% (10/35), 35% (6/17) vs 6% (2/35), and 59% (10/17) vs 11% (4/35), with a significant difference (P <0. 01), but no statistical difference existed between indices such as abdominal pain, vaginal bleeding and abdominal tension ( P > 0. 05 ). (5) Placenta sites and abruption areas: placenta sites were distributed from anterior or posterior of uterine body 5/17 vs 24/35 , the fundus or cornu of uterus 12/17 vs 11/35 ( P < 0. 01 ). All cases in observation group presented abruption areas> 1/3, and 9 cases ≥2/3, 27 cases abruption areas < 1/3 and 8 cases abruption areas 1/3 -2/3 in control group (P<0.01). (6) Other complications and outcome: Hemorrhagic shock 3 vs 0, DIC 3 vs 0, hysterectomy 1 vs 0, intrauterine fetal death 3 vs 2, neonatal asphyxia 8 vs 5 and neonatal death 1 vs 0. There were significant differences ( P < 0. 01 ) between the two groups. Conclusions Preeclampsia, long duration of disease and fundal or cornual placenta a risk factors for uteroplacental apoplexy complicating placental abruption, which may lead to a poor maternal-fetal prognosis.

Objective:To determine the related substances in dexibuprofen and its sustained release tablets by HPLC .Methods:The related substances including a-methyl-4-butyl benzene acetic acid , L-ibuprofen and the others were detected by HPLC .An Agilent Eclipse XDB-C18 column (150 mm ×4.6 mm, 5 μm) was used for a-methyl-4-butyl benzene acetic acid and the other related sub-stances.A Kromasil 5-TBB column (250 mm ×4.6 mm, 5 μm) was used for L-ibuprofen.The mobile phases was acetonitrile and phosphate solution (45 ∶55, pH value was adjusted to 2.5 by phosphoric acid), hexane-tert-butyl ether-acetic acid (850 ∶150 ∶1), acetonitrile and sodium acetate buffer (60 ∶40, 6.13g sodium acetate was dissolved in 750ml water, and pH was adjusted to 2.5 by glacial acetic acid ) , respectively .The detection wavelength was 214 nm, 220 nm and 263 nm, respectively .The flow rate for a-meth-yl-4-butyl benzene acetic acid and the other related substances was 1.0 ml· min-1 .The flow rate for L-ibuprofen was 2.0 ml· min-1 . The column temperature was 30℃and the injection volume was 20 μl.Results:The method could effectively detect the contents of a-methyl-4-butyl-phenylacetic acid and L-ibuprofen, and identify and separate the related substances in dexibuprofen raw material and its sustained release tablets .Conclusion:The method is specific , accurate and easy to operate , which can be used for the quality control of ibuprofen raw material and its sustained release tablets .

Background and purpose:The incidence of lung cancer with brain metastasis tends to go up. The technique of intensity-modulated radiotherapy (IMRT) with simultaneous integrated boost (SIB) for tumor bed is a new treatment choice for patients with brain metastasis. This study aimed to investigate the feasibility of IMRT combined with SIB for tumor bed in the treatment of brain metastases of lung cancer by exploring its short-term clinical effect and treatment-related toxicities.Methods:Thirty-two patients who were diagnosed as having lung cancer with 1-4 brain metastases were prospectively collected. All the brain metastases were treated with whole brain 5 ifelds IMRT (40 Gy/20 f) combined with SIB (50 Gy/20 f). The dosage distribution within the target volume and dose-volume histogram were evaluated. Meanwhile, the overall response rate of radiotherapy, thelocal tumor control rate, 1-year survival rate, treatment-related acute and late toxicities were also calculated.Results:All patients were treated with IMRT successfully. The rate of Grade 2 vomitting, nausea, epilepsy was 9.4%, 15.6%, and 12.5%, respectively. Two cases had Grade 2 cognitive impairment, 4 cases had Grade 2 memory injury, and 2 cases had Grade 3 memory injury. There were no any Grade 4 events of acute and late toxicities. The overall response rate of radiotherapy was 71.9%. The local tumor control rate was 96.9%, and 1-year survival rate was 44%.Conclusion:It is feasible to use 5 ifelds IMRT (40 Gy/20 f) combined with SIB (boosting 10 Gy, i.e. total dose 50 Gy/20 f) for the treatment of brain metastases of lung cancer.

Objective To investigate the application effect of quality control management in the prevention of indwelling catheter associated urinary tract infection in intensive care unit (ICU) patients. Methods Cases of patients stayed in the department of ICU undergoing indwelling catheter over 10 d were selected by using time stage sampling method. Totally 136 cases of patients were selected from January 1, 2012 to December 31, 2012, as the control group. A total of 145 cases of patients were selected from January 1, 2013 to December 31, 2013, as the performance group. Cases in performance group were taken standardized training and quantify the performance appraisal on the basis of measures in the control group. The urine routine was tested and bacterial was cultured at indwelling catheter 3 d, 7 d and 10 d, respectively. The indwelling catheter associated urinary tract infections of the two groups were compared. Results After the implementation of the performance appraisal management, the incidences of indwelling catheter associated urinary tract infection at 3 d, 7 d and 10 d were 4.8%(7/145), 19.3% (28/145) and 32.4% (47/145), respectively. Within each quarter, the incidence of indwelling catheter associated urinary tract infection was rising with indwelling catheter time prolonged. And the incidences of the first and second quarter were higher than three and four quarter. The incidences of indwelling catheter associated urinary tract infections at 3 d, 7 d and 10 d in the performance group were lower than the control group, the differences were statistically significant (χ2=4.494, 30.660 and 49.307, P < 0.05). Conclusions Standardized training of nursing staff in ICU and implementation of performance appraisal management could effectively improve the enthusiasm and sense of responsibility of the nurses, and effectively reduce the incidence of indwelling catheter associated urinary tract infection.