Gastroesophageal reflux disease and sliding hiatal hernia are chronic disease,and sometimes there are not typical clinical symptoms.Patients don't have enough cognition or put enough em-phasis on it.Determining the sliding length of the sliding hiatal hernia and gastroesophageal reflux disease respectively by means of high resolution manometry and 24 hour pH monitoring,in turn,analyzing the rela-tionship between sliding length of the sliding hiatal hernia and gastroesophageal reflux disease.

Recent studies suggest that astrocyte elevated gene 1 ( AEG-1 ) is almost highly expressed in all types of malignant solid tumors and correlates with poor prognosis,which becomes a prognostic marker for many kinds of tumors.As a strongly basic protein,AEG-1 possesses a transmembrane domain and multiplenuclear localization signals.It is present in the cell membrane,cytoplasm and nucleus.As an oncogene,AEG-1 palys an important role in a virety of malignant biological behaviors of cancer,which range from cell proliferation,apoptosis,migration,adhesion,invasion to tumor angiogenesis and chemotherapy resistance.Its critical role in tumor genesis and progression has made it a potential therapeutic target.

A series of the positive cord dorsum potentials (CDP)were recorded in the dorsal surface of the spinal cord when the region of the nucleus raphy magnus (NRM)was stimulated by electricity. This CDP (NRM—CDP) consisted of three potentials. They were a Compound potential with a short time course and two slow potentials with a long time course which followed the compound one. The characteristics of the slowly potentials—NRM—CDP—1 and NRM—CDP—2 waves were analysed the parameters concerned were recorded. Therefore, a good experiment animal model and new information are provided for the further a study of the NRM action.

Objective To reach parameter on sterilization wrap of medical instrument. Methods The parameter of one hundred twenty sterilization wraps with size 30 cm?40 cm?60 cm and weight 20kg was tested by Belimed sterilizer. Results There are 155 masculine gender and 445 negative in 600 quick reading biological indicators of 120 sterilization wraps and 53 masculine in bacteria detection, 9 cases failure in 1243 indicator, 52 wet wraps. Conclusion The parameter was to take three times negative pressure pulse and five times pressure pulse, sterilization temperature 134℃, sterilization time 11min and dry time 11min, matching to sterilization request of "Technical Standard For disinfection".

Asthma ; drug therapy ; metabolism ; Cytokines ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Humans ; Inflammation Mediators ; metabolism ; Phytotherapy ; Signal Transduction

OBJECTIVE:To observe clinical efficacy and safety of Chaihu shugan powder combined with clonazepam in the treatment of liver qi stagnation type anxiety. METHODS:96 patients with liver qi stagnation type anxiety were randomly divided in-to observation group and control group,with 48 cases in each group. Control group was given Clonazepam tablet with initial dose of 0.5 mg,increasing to 4.0 mg gradually,tid;observation group was additionally given Chaihu shugan powder 300 ml,bid,on the basis of control group. Both groups were treated for 6 weeks. Clinical efficacy of 2 groups were observed,and HAMA and SAS were observed before and after treatment;the incidence of ADR were compared between 2 groups. RESULTS:The total effective rate of observation group(97.92%)was significantly higher than that of control group(83.33%),with statistical significance(P<0.05). There was no statistical significance in HAMA and SAS between 2 groups before treatment(P>0.05);HAMA and SAS of 2 groups decreased significantly 1,3 and 6 weeks after treatment,and the observation group was lower than the control group, with statistical significance(P<0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:Chaihu shugan powder combined with clonazepam is effective,improve patient anxiety and safe in the treatment of liver qi stagnation type anxiety.

Enzyme-linked immunosorbent assay ( ELISA) was applied to detect the antibody against CoxA16 and EV71 among 0~14 age groups. In 1 000 cases of children, the total positive rate of anti-EV71 IgM was 19. 9%, the total positive rate of anti-EV71 IgG was 47. 7%, the total positive rate of anti-CoxA16 IgM was 22. 8%, the to-tal positive rate of anti-CoxA16 IgG was 51. 3%. There was a negative correlation between positivity for EV71 IgM and positivity for CoxA16 IgM(χ2 =2. 794,P>0. 05). There was also a negative correlation between positivity for EV71 IgG and positivity for CoxA16 IgG(χ2 =2. 793,P>0. 05). The positive rate of IgG antibody against CoxA16 and EV71 was higher in neonatal group in which anti-EV71 IgG was 41 . 5% and anti-CoxA16 IgG was 49 . 5%. The lowest positive rate of IgG antibody against CoxA16 and EV71 was in infant group in which anti-EV71 IgG was 38. 0% and anti-CoxA16 IgG was 43. 5%. Except for neonatal group,the positive rate of IgG antibody against Cox-A16 and EV71 increased gradually with the age growth, there were statistical differences among the age groups (χ2=27. 04 and 19. 98,P<0. 01). With the age growth,the positive rate of IgM antibody against EV71 and CoxA16 increased,there were statistical differences among the age groups(χ2 =41. 12 and 37. 13,P<0. 01). There were not statistical differences between gender groups (χ2 =0. 51,1. 77,0. 36 and 2. 12,P>0. 05).

Objective To compare the diagnosic performance of conventional 2D and 3D Cube MR imaging to identify complete and partial tears of anterior cruciate ligament(ACL).Methods Farty patients suspected of having tears of anterior cruciate ligament were prospectively studied by 3.0 T conventional 2D and 3D Cube MR imaging and arthroscopy.MR images were interpreted in consensus by two experienced radiologists,and the ACL was diagnosed as being normal,partially torn,or completely torn.Diagnostic accuracy of 2D and 3D Cube MR for the detection of both complete and partial tears of ACL was calculated using arthroscopy as the standard of reference.Area under curve (AUC) of both methods were calculated using ROC curves and were compared using Hanley& McNeil curve comparisions.Results Sixteen patients had intact ACL,12 had complete tear,and 12 had partial tear of the ACL at arthroscopy.For complete ACL tear,AUC of 2D MR and 3D Cube was 0.839 and 0.923 respectively,and there was no significant difference on ROC curves (Z =1.245,P =0.213).For partial ACL tears,AUC of 2D and 3D Cube MR were 0.643 and 0.881 respectively,and there was significant difference on ROC curves (Z =2.384,P =0.017).Conclusions Both 2D MR and 3D Cube MR have high sensitivity and specificity for identifying the complete ruptures of ACL.3D Cube MRI appears to be superior inidentifying partial rupture of ACL.

Objective To investigate the effects of remifentanil on lipopolysaccharide ( LPS)-induced acute lung injury (ALI) in rabbits.Methods Thirty healthy male New Zealand white rabbits weighing 2.5-3.5 kg were randomly divided into 5 groups ( n = 6 each) : group Ⅰ control (group C) ;group Ⅱ ALI;group Ⅲ, Ⅳ, Ⅴ low, median and high dose RF + LPS (group LR, MR, HR) . The animals were anesthetized with intravenous 3% pentobarbital sodium 30 mg/kg, tracheostomized and mechanically ventilated. The carotid artery and jugular vein were cannulated for MAP and HR monitoring, blood sampling, and fluid and drug administration. LPS 0.5 mg/kg in 10 ml of normal saline (NS) was infused over 30 min in group Ⅱ-Ⅴ. Remifentanil 0.2, 0.4 or 0.8 μg·kg~(-1)·min~(-1) was infused starting from 15 min before LPS administration until the death of the animals. MAP, HR, peak airway pressure (P_(peak) ), PaO_2 and plasma intercellular adhesion molecule 1 (ICAM-1) concentration were measured immediately before LPS infusion (T_0, baseline) and at 1, 2.5 and 5.5 h after the end of LPS infusion. The animals were killed and the lungs were immediately removed for microscopic examination and determination of W/D lung weight ratio. Results MAP, HR and PaO_2 were significantly decreased while W/D ratio and P_(peak) were significantly increased after iv LPS infusion as compared with control group. LPS significantly increased plasma ICAM-1 concentration and damaged the structure of lung tissue. Remifentanil infusion significantly attenuated the LPS-induced changes in a dose-dependent manner. Conclusion RF has protective effect against LPS-induced ALI and inhibition of ICAM-1 expression is involved in the mechanism.