Evidence-Based Medicine ; Humans ; Rhinitis, Allergic, Perennial ; drug therapy ; Rhinitis, Allergic, Seasonal ; drug therapy

Child ; Humans ; Rhinitis, Allergic, Perennial ; diagnosis ; therapy

Child ; Humans ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial

Humans ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; diagnosis

Objective:To observe the therapeutic effect of the method of clearing lung and descending stomach and its mechanism on gastroesophageal reflux cough(GERC).Method:125 cases of GERC were divided randomly into the treatment group(n=65) treated by the therapy of clearing lung and descending stomach(with a self-designed decoction) and the control group(n=60) treated with Omeprazole and Domperidone,and they were all treated for 12 weeks.Results:The total effective rate was 92.3% in the treatment group and 78.3% in the control group,with a significant difference between the two groups(P

Asthma ; psychology ; therapy ; Humans ; Integrative Medicine ; Stress, Psychological

Humans ; Medicine, Chinese Traditional ; Respiratory Tract Diseases ; prevention & control ; therapy ; virology ; Virus Diseases ; prevention & control ; therapy

Objective To evaluate the influence of expression of pre S1 protein on the genomic expression of hepatitis B virus (HBV) infected hepatocyte with microarray. Methods The differentially expressed genes between the hepatoblastoma cell line HepG2 transfected by pcDNA3.1(-) and pcDNA3.1(-)-preS1, were respectively compared by cDNA microarray technique. The HBV pre-S1 coding DNA fragment was amplified with polymerase chain reaction (PCR) technique by using G376-7 plasmid DNA containing the full length of HBV genome as the template. The expressive vector of pcDNA3.1-preS1 was constructed by routine molecular biological methods. HepG2 cells were transfected by pcDNA3.1(-) and pcDNA3.1-preS1, respectively, using FuGENE6 Transfection Reagent. The total RNA was isolated and reversely transcribed. Results The cDNAs were subjected for microarray screening with 1152 cDNA probes. From the scanning results, it was found that 30 genes were up-regulated and 38 genes were down-regulated by pre-S1 protein of HBV. Conclusion The expression of pre-S1 protein affected the genomic expression spectrum of HBV infected hepatocyte(HepG2 cell line).

Animals ; Diabetic Nephropathies ; drug therapy ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Hypoglycemic Agents ; pharmacology ; Phytotherapy

Female ; Humans ; Infant ; Metabolism, Inborn Errors ; Methylmalonic Acid ; blood