OBJECTIVE:To investigate the paracrine effects of bone marrow mesenchymal stem cells on osteoblast biological function. METHODS:Bone marrow mesenchymal stem cells were isolated using standard Ficol-Paquedensity gradient centrifugation. Mesenchymal stem cellconditioned medium was prepared to cultivate osteoblasts, MG63. Proliferation of MG63 cells was analyzed by cellcounting kit-8. Migration of MG63 cells was analyzed by cellscratch method. Alkaline phosphatase activity of MG63 cells was analyzed by microplate test kit. Real-time PCR was performed to evaluate osteoblast differentiation markers, alkaline phosphatase, col agen type I and osteocalcin. Alizarin red staining was performed to evaluate osteoblast mineralization. RESULTS AND CONCLUSION:The cells were strongly positive for CD44, CD73 and CD90, but negative for CD34. MG63 cells cultured in the conditioned medium showed better proliferation and migration than those cultured in the Dulbecco’s modified Eagle’s medium. The activity and mRNA expression of alkaline phosphatase were much higher after induction of 4, 7 days (P<0.01). There was no significant difference in expression of col agen type I and osteocalcin after induction of 4 days, but they were significantly higher than those in the control group after induction of 7 days (P<0.05). Alizarin red staining showed that the number of calcium nodules was increased and the mineral apposition was enhanced after induction of 21 days with the conditioned medium. These findings suggest that the paracrine substance of bone marrow mesenchymal stem cells can significantly promote osteoblast proliferation, migration, differentiation and mineralization.

Objective To systematically review the effect of picture exchange communication system (PECS) on social communication and speech in children with autism spectrum disorder (ASD). Methods Literatures were retrieved from PubMed, Medline, EBSCO, Elsvier, VIP, CNKI, evaluated and screened out. Results Six articles were included in the systematic review. Two of them researched the effect of PECS on social communication and speech, two for social communication and other two for speech. Four of them supported that PECS might promote the social communication, three of them supported that PECS promote the speech, and one indicated that PECS did less for speech. Conclusion PECS benefits social communication in children with ASD, especially for the request, and may facilitate the increase of the vocabulary and speech. The characteristics of children with ASD may associate with the efficacy of PECS.

Objective To evaluate 18F-FDG PET/CT imaging in primary adrenal lymphoma (PAL). Methods Three male patients with clinically diagnosed PAL, aged 59 to 77 years old, were examined by18F-FDG PET/CT before histopathologic examination. The SUVmax ratio of tumor to liver (T/L)was calculated on 18F-FDG PET images. All patients underwent R-CHOP ( rituximab-cyclophosphamide,doxorubicin, vincristine, and prednisone) chemotherapy regimens. One patient had serial follow-up 18F-FDG PET/CT post therapy. Results The final diagnosis of all 3 cases was bilateral diffuse large B-cell lymphoma. The pathologic results of bone marrow biopsy were negative. Case 1 was in disease remission based on four times of follow-up 18F-FDG PET/CT. The patient's clinical follow-up was also negative and he was currently alive for more than 19 months. Case 2 was of late stage and suffered from adrenal insufficiency. He died 6 months after the diagnosis. Case 3 (the eldest patient) was treated with partial excision followed by the chemotherapy. He died of lung infection 12 months after treatment. Conclusion 18 F-FDG PET/CT may be of important value for maximizing patient management and survival for patients with PAL, a rare and highly malignant disease.

Objective To observe the curative effects of metformin combined with cyproterone therapy on polycystic ovary syndrome(PCOS). Methods Ninety diagnosed PCOS patients were treated. The serum luteinizing hormone(LH), luteinizing hormone and follicule stimulating hormone(LH/FSH) ratio and T, waist hip ratio(WHR) of the patients before therapy were compared with using merformin combined with- cyproterone three cycles. Results A significant reduction(P<0.05 or P<0.01) in LH,LH/FSH,T and WHR of patients after the therapy was observed. Menses became regular after the therapy, and ache and hirsutism mitigated. Conclusion Metformin com- bined with cyproterone is a perfect means to treat polycystic ovary syndrome(PCOS).

Advances in resuscitation and intensive care have led to high rates of survival among neonates with life -threatening conditions such as asphyxia,prematurity and congenital malformations.The sequelae of neurologic con-ditions arises in the neonatal period include lifelong disabilities such as cerebral palsy,epilepsy,intellectual and beha-vioral disabilities.There is an increasing demand for resource -intense strategies for acute neurological care within neo-natal intensive care unit.Neonatal neurocritical care is a multidisciplinary subspecialty that combines expertise in neo-natology,pediatric neurology,radiology,rehabilitation,surgery,and has led to improved outcomes in newborn that have critical illnesses.Neonatal neurocritical care focus the needs of the developing newborn brain,including attention to physiology to help prevent secondary brain injury,a protocol -driven approach for common conditions such as hypoxic -ischemic encephalopathy and seizures,education of specialized teams that use brain monitoring and imaging to evaluate the effect of critical illness on brain function.

Purpose To investigate the MRI findings and to improve the understanding of spinal epidural angiolipomas. Materials and Methods MRI findings were retrospectively analyzed in two cases of surgery and pathology proven spinal epidural angiolipomas. Relevant literatures were reviewed. Results Two cases of spinal epidural angiolipomas were located in the lumber and lower thoracic spinal canal. The tumors were elongated or spindle in shape within the epidural space, and parallel to the longitudinal axis. In both cases part of the tumors were hyperintense on T1WI and T2WI, attenuated on fat-saturated sequences with mild enhancement. Some areas were hypointenseon T1WI, hyperintenseon T2WI, unattenuated on fat-saturated sequences with apparent enhancement. Angiolipomas were classified according to the MRI features. One was type I and the other was type III. Both cases were non-infiltrative. Conclusion MRI shows the size, shape, MRI signals and the association with the adjacent structure of spinal epidural angiolipomas. The MRI classification is helpful for improving the understanding of the tumor. MRI is the best imaging modality to diagnose spinal epidural angiolipomas.

Objective To observe the clinical effects of the combination of resuscitation-inducing acupuncture therapy and Naoluotong Recipe ( NR) for the treatment of acute cerebral infarction. Methods By using simple random method, 70 qualified patients were evenly divided into combination group and control group. Both groups were given conventional western medicine treatment and Xiangdan Injection, and additionally, the combination group was given resuscitation-inducing acupuncture therapy and oral use of NR decoction. After treatment, the two groups were evaluated by the United States National Institute of Health Stroke Scale ( NIHSS) scoring and Barthel index ( BI) for independent living ability. Results ( 1) The total effective rate showed by NIHSS scoring was 93. 3% in the combination group and was 72.4% in the control group (P<0.01). ( 2) The results of BI evaluation showed that the disability rate was 43.33% in the combination group and was 75.86%in the control group (P<0.01). (3) After treatment, NIHSS scores in both groups were decreased while BI scores were increased (P<0.05 compared with those before treatment), and the improvement of the scores in the combination group was superior to that of the control group ( P<0.05). Conclusion The combination of resuscitation-inducing acupuncture therapy and oral use of NR decoction can significantly improve neural function and reduce disability rate of patients with acute cerebral infarction, and the curative effect is superior to western medicine alone for the treatment of acute cerebral infarction.

Objective To investigate the effect of electroacupuncture on hypothalamic and hippocampal expressions of ghrelin and ghrelin receptor (GHS-R) mRNA in functional dyspepsia (FD) rats. Methods Eighty SD rats were randomly allocated to normal control, model control, medication and electroacupuncture groups, 20 rats each. A rat model of FD was made by tail clamp modeling method. The medication group received an oral gavage of cisapride solution and the electroacupuncture group, electroacupuncture treatment. The expressions of ghrelin protein and GHS-R mRNA in the hypothalamus and hippocampus were examined by Western blot and real-time PCR, respectively, in all the four groups. Results Compared with the model control group of rats, the expression of ghrelin protein in the hypothalamus and the expression of GHS-R mRNA in the hypothalamus and hippocampus increased in the medication group (P<0.05) and the expressions of ghrelin protein and GHS-R mRNA in the hypothalamus and hippocampus were up-regulated in the electroacupuncture group (P<0.05). Compared with the medication group of rats, the expressions of ghrelin protein and GHS-R mRNA in the hippocampus were up-regulated in the electroacupuncture group (P<0.05). Conclusions Electroacupuncture and medication can improve gastrointestinal functions and correct an imbalance in the brain-gut axis through the hippocampus-hypothalamic pathway by regulating hypothalamic and hippocampal ghrelin protein and GHS-R mRNA contents and activating hypothalamic neurons and hippocampal excitatory synapses.

Objective: To analyze the Effect of rt-PA thrombolytic therapy on elderly patients with cerebral infarction. Methods: Chosen elderly patients with cerebral infarction between November 2012 to November 2013 as study object, randomly divided into observe group receive rt-PA thrombolytic therapy and control group receive aspirin and Batroxobin, detecting neurological impairment, physical function score and adverse treatment outcomes. Results: 1)before thrombolytic treatment, neurological deficit situation of two groups were no significant difference (P>0.05), after treatment, observation group patients’ neurological deficit scores were significantly lower than control group at each time point (P<0.05); 2)after treatment, limb function defective number of observation group patient was significantly less than observation group(P<0.05); 3)after treatment, observation groups’ adverse outcomes as again infarction, large area cerebral hemorrhage after thrombolysis, plant survival, incidence of death was significantly lower than control group (P<0.05). Conclusion: The injection of rt-PA thrombolytic therapy can significantly optimize neurological and physical function in patients , reducing the incidence of adverse treatment outcomes, with positive clinical significance.

Trojan peptides,also named cell-penetrating peptides or protein transduction domains,are a class of small cationic peptides that often contains less than 30 amino acids.They can deliver a wide range of "cargos" such as peptides,proteins and nucleic acids efficiently through the cellular membrane.This review mainly focuses on the recent progress on utilizing Trojan peptides to deliver plasmid DNA and siRNA into cells in vitro and in vivo,and also highlights the implications of this technology in both gene function study and therapeutic potential.