Aim To study the inhibitory effect of Oxymatrine-Baicailin compound on the secretion of hepatitis B viral antigens in HepG 2.2.2.15 cells.Methods HepG2.2.2.15 cells were cultured and treated with a series of Oxymatrine,Baicailin,or Oxymatrine-Baicailin compound respectively.The toxicity effect was determined by MTT colorimetry.Con-tents of the hepatitis surface antigen(HBsAg) and hepatitis e antigen(HBeAg) in the culture supernatants were determined by Enzyme linked immunosorbent assay.Results Oxymatrine at concentrations between 0.125 and 1 g?L~(-1) had little toxicity effect on cells,but Oxymatrine at 2 g?L~(-1) and 4 g?L~(-1) had much more toxicity effect on cells.The inhibitory effect of Oxymatrine on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1).The toxicity of Baicailin on cells increased from 0.625 to 2 g?L~(-1),especially when the dose surpassed 1 g?L~(-1).The inhibitory effect of Baicailin on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1),but its efficacy was inferior to Oxymatrine's efficacy.Oxymatrine-Baicailin compound had good inhibitory effect on hepatitis B viral antigens secretion,and the inhibition effect of the compound on HBeAg was superior to the effect on HBsAg.The Group C Oxymatrine-Baicailin compound had good synergism inhibition effect on hepatitis B viral antigens secretion and the inhibition rate of the specific group compound was significantly superior to that of Oxymatrine treatment alone(HBsAg:P=0.043;HBeAg: P=0.026).Conclusion Oxymatrine-Baicailin compound has good synergism effect on hepatitis B viral antigens secretion in HepG2.2.2.15 cells.

Animals ; Breast Neoplasms ; pathology ; Cell Division ; physiology ; Cells, Cultured ; Humans ; Macrophages ; cytology ; Signal Transduction ; immunology ; physiology ; Space Flight ; Tumor Cells, Cultured ; Weightlessness

Burns ; therapy ; Fluid Therapy ; Humans

Objective To study the expressive levels of KAI-1mRNA,Kiss-1mRNA and MTA1 in the benign and malignant lesions of breast,and their clinicopathological significance.Methods We detected the expression levels of KAI-1mRNA,Kiss-1mRNA and MTA1 in the sections from 60 cases of breast cancer and 30 cases of benign lesions of breast.Results The positive rates and scores of KAI-1mRNA and Kiss-1mRNA were significantly lower in breast cancer than those in benign lesions(P

Renal Tubular Cell Apoptosis refers to activation,proliferation,distension,cast formation and cellular apoptosis of the tubular epithelium when the renal tubule undergoes hypoxia,poisoning,inflammatory reaction,plasma protein or amylaceum.This text summarized the researches on the prevention and control of lenal tubular cell apoptosts in renal interstitial fibrosis in the recent years.

AIM: To investigate the adsorption mechanism of pueraria on the molecular imprinting polywer(MIP) and Scatchard analysis was carried out. METHODS: Pueraria MIP was synthesized using pueraria as the template,acrylamide as the functional monomer and ethylene glycol dimethaerylate(EDMA) as the crosslinker. RESULTS: The result showed that at least two classes of binding sites were formed in the imprinted polymer.Extracting compounds from Radix Pueraria was separated by MIP. CONCLUSION: The component of the products is analyzed by HPLC.The result shows that the final products contain pueraria and the other two kinds of isoflavone.

In the paper, a microtiter plate method was used to determine the residual prekallikrein activator(PKA) activity in blood products.The result of study indicated that it would be satisfactory that the reaction mixture of PKA and prekallikrein (PK) was iucul ?? at room temperature for 30 minutes with 0.05M Tris/0.15M NaCl buffer(pH 8.0) , and then the mixture continued to be incubated at room temperature for another 15 minutes after the chromogenic substrate for kallikrein was added. The method had a good reproducibility. The PKA contents determined in IVIG agreed with that of the processing method of IVIG and the hypotensive test of rats, and were basically consistent with the result, determined by the method developed by the Finnish Red Cross Blood Transfusion Service.

Objective To investigate the effect of mytomycin C in the operation of dacryocystorhinostomy for curing lacrimal passage obstruction. Method Forty-one patients with traumatic lacrimal passage obstruction were divided into treatment group (n=21, 14 males and 7 females with an average age of 32) and control group. (n=20, 12 males and 8 females with an average age of 35). The patients in treatment group received 0.4mg/ml of mytomycin C when undergoing dacryocystorhinostomy, while the patients in control group received no medicine when undergoing the same operation. Lacrimal passage was cleaned with water after operation, and the operational effectiveness of dacryocystorhinostomy were compared between the treatment group and control group. Results Twenty-one eyes were cured in treatment group, and the cure rate reached 100%. While for the 20 eyes in control group, 17 eyes were cured, and the cure rate was 85%. There existed statistical significance between the two groups (P

Objective To induce experimental autoimmune myasthenia gravis (EAMG) rabbits by using T?125～147 Methods Peptides T?125～147 were synthesized referring to the residue sequence of acetylcholine receptor of Torpedo California and the rabbits were inoculated with the peptides Clinical manifestation was graded in 4 levels Electrophysiological function was assessed by repetitive nerve stimulation (RNS) and single fibre electromyography (SFEMG) tests Anti peptides antibodies were evaluated by enzyme linked immunosorbent assay (ELISA) Student t test was used to analyze the difference between the EAMG and healthy rabbits Results Following the second inoculation,the rabbits appeared weakness Clinical symptoms were improved by neostigmine At 3,5 and 10 Hz,the decrement of compound muscle action potential (CMAP) and the mean jitter (MCD) of the immunized rabbits were higher than in the healthy ones The percentage of decrement of CMAP in order of the control group and the T?125～147 group were:3 Hz: 1 625?1 317,25 375?7 945; 5 Hz: 2 000?1 732,25 625?9 102; 10 Hz: 1 750?1 392,28 875?8 709.Following the above sequence,the MCD were:3 Hz: (9 875?1 126) ?s,(25 875?7 945) ?s; 5 Hz: (11 375?0 916) ?s,(27 500?3 381) ?s; 10 Hz: (12 375?1 061) ?s,(31 000?4 811) ?s Anti peptide antibodies in immunized groups were significantly higher than those in the control group Conclusion T?125～147 might be served as the immunogenic to induce EAMG in rabbits,accompanied by elevation of anti peptide antibodies and the blockage of neuromuscular transmission

Objective To discuss the diagnosis and clinical value of spiral CT(SCT) in orbital blowout fracture.Methods The SCT MPR findings in conjunction with transaxia images in 120 cases of orbital blowout fracture were retrospectively analyzed. According to the this analysis,108 patients were treated conservatively only 12 were under the opereation.Results Interruption of bone continuity, fragments, depression and displacement of orbital bone wall were direct signs of orbital blowout fracture." Tear drop"sign in the maxillary or ethmoid sinus was a characteristic indirect sign of inferior or medial orbital wall fracture. Enophthalmos obviously compacting of extraocular muscler and distraction test male were operation signs.Conclusion Orbital blowout fracture can be diagnosed by SCT volume scanning findings of the MPR comprehensively and accurately, that is very important in choosing the treatment methods for orbital blowout fracture.