Objective In order to understand the mechanism of solute transperitoneal transport, we studied the relationship between IL-8 level and protein transperitoneal transport. Method 12 New Zealand White rabbits were randomly divided into experimental and control groups. 2 5% glucose peritoneal dialysis solution (40ml/kg)contained 3?10 9 CFU staphylococcus aureus 1ml was injected into the abdominal cavity of experimental NZW rabbits and 2 5% glucose peritoneal dialysis solution (40ml/kg)contained 0.9%NaCl solution 1ml was injected into the abdominal cavity of controlling NZW rabbits. The plasma and effluent concentrations of blood creatinine ,glucose,total proteins and albumine were determined respectively, the D/P or D/Do values of creatinine , total proteins ,albumine or glucose were calculated respectively. Results The D/P ratios of creatinine, total proteins and albumin were increased significantly, while the D/Do of glucose was decreased in experimental group on different dwelling time points ,there was a significantly difference,as compared with the control group (P0 05),while there was significantly correlation after 60 mins(P

Breath Tests ; Humans ; Mining ; Pneumoconiosis ; diagnosis ; Radon ; analysis

OBJECTIVE: To establish the preparation method and detection technology of ribavirin polymorphism, and to estimate the bioavailability differences among the ribavirin polymorphs. METHODS: Through polymorphism screening, ribavirin crystals of the form A, B, C, D were obtained. The crystal forms of ribavirin were characterized by different analysis methods, such as single crystal X-ray diffraction method (SXRD), powder X-ray diffraction method (PXRD), differential scanning calorimetry method (DCS), infrared spectrum method (IR) and melting point method (MP). solid ribavirin in different forms were orally administered to rats, and an HPLC-MS method was established to determine the plasma levels of ribavirin, and the bioavailability was analyzed. RESULTS: Of the four polymorphs of ribavirin, form C and form D were reported for the first time. The four polymorphic forms all could be identified by PXRD, DSC, IR and MP. The ρmax and AUC0-t of form A were superior to those of the other forms. CONCLUSION: Characteristic data for ribavirin polymorphism are obtained; and form A of ribavirin is a suitable medicinal crystal. This study has provided the basis for choice of medicinal crystal and the improvement of quality standards.

Hearing Loss ; epidemiology ; Humans

Objective To construct the eukaryotic expression vector of HBEBP2 gene,and screen the exonic genes of proteins in hepatocytes interacting with HBEBP2.Methods The DNA fragment of HBEBP2 was amplified by PCR.The eukaryotic expression vector pGBKT7-HBEBP2 was constructed successfully by yeast-two hybrid system 3 and then transformed into yeast cells AH109.The transformed AH109 were then mated with yeast cells Y187 containing hepatic cDNA library plasmid.The diploid yeast cells were plated on synthetic dropout nutrient medium(SD/-Trp/-Leu/-His/-Ade) containing X-?-gal for the first selection.Library plasmids pACT2-DNA were extracted and co-transformed into yeast cell AH109 together with pGBKT7-HBEBP2.Then the yeast cells were plated on synthetic dropout nutrient medium(SD/-Trp/-Leu /-His/-Ade) containing X-?-gal for the second screening to eliminate false positive clones.The real positive clones were sequenced and analyzed by bioinformatics.Results Six proteins binding to HBEBP2 were screened,including human sapiens lactate dehydrogenase D,human sapiens mitochondrion,human sapiens mannose,human sapiens aldehyde oxidase,human sapiens serpin peptidase inhibitor and other two unknown proteins.Conclusions A novel class of proteins in hepatocytes interacting with HBEBP2 has been obtained.It is presumed that HBEBP2 protein is correlated with glycosylation,lipid metabolism and cell proliferation,etc.

Objective To identify the molecular components involved in acquired methotrexate-resistance of human choriocarcinoma. Methods Methotrexate-resistant cell line(JAR/MTX) was derived from JAR cell line by exposed to intervally and progressively increasing higher concentration of MTX. cDNA microarray analyses of JAR/MTX and its parental cell line JAR were performed. Results JAR/MTX was established after one year with stable resistance. Its resistant index to MTX was 7.3. Nine genes were differential expressed between JAR/MTX and JAR cells. INSR,SLC1A3,SAT,HBB,and FLJ12443 were underexpressed and HS1,TXNRD1,TAGLN2,and EEF2 were overexpressed in JAR/MTX cells. Conclusion The cDNA microarray system showed that several alterations of gene expression were present in acquired methotrexate-resistance of human choriocarcinoma.

The protective effect of selenium (So) on the subacute cardiomyopathy was investigated in the mouse given a single dose of adriamycin (ADR 17. 5mg/kg ip). Se pretreatment (80?g/kg?10 ip) produced protection against the ADR-induced early myocardial damage associated with increased permcability of cell membranes as evidenced by apparent D- PAS (diastase- periodic acid-Schiff) staining material noted in the sarcolemma. The protective effect of Se could be partially explained as the detoxication of lipid peroxides via the action of enhanced cardiac Se-glutathione peroxidase.

Objective To observe the changes of macula in patients with high myopia after phacoemulsification. Methods In 20 patients with high myopia with ocular axial length≥27 mm, optical coherence tomography (OCT) was performed on the operative and contralateral eyes 1 week before and after monocular phacoemulsification, respectively, and the OCT images of macula of the operative eyes were observed and compared. Results One week before and after phacoemulsification, the mean macular fovea thickness of the patients with high myopia was (131.6?16.37) ?m and (189.75?45.69) ?m, respectively, with a significant difference (t=2.805, P=0.01). Simultaneously, the mean macular fovea thickness of the contralateral eyes was (133.5?15.12) ?m and (133.5?14.63) ?m, respectively, with a non-significant difference (t=1.367, P=0.853). In 20 operative eyes 1 week after phacoemulsification, 3 had vitreous strand around the macula with retinal thickening, 1 had retinoschisis in macular area, and 2 had obvious retinal thickening with slight retinal edema. Conclusion Retinal thickening occurs in the patients with high myopia after phacoemulsification. Traction of retina by vitreous strand or subclinical retinoschisis may occur in some patients.

Objective To investigate telomerase activity in patients with psoriasis. Methods The telomerase activity in the skin lesions of patients was detected by TRAP (Telomeric repeat amplification protocol)——ELISA which is based upon PCR amplification of the initial telomerase product and detected by ELISA. Results Telomerase activity was detected in the lesions of psoriatic patients, but the level of it was lower in comparison with that in tissues of malignant tumor and K562 cell line. Conclusion These findings support that the increase of telomerase activity may be linked to the pathogenesis of psoriasis and that the telomerase activity can be detected not only in malignant tumors but also in nonmalignant skin diseases.

Objective To analyze the diagnostic and therapeutic process of 3 cases of relapsing polychondritis(RP) and explore strategies for early diagnosis and effective therapy.Methods With retrospective study on three relapsing polychondritis patients in our hospital,we observed their major manifestations at admissions and summarized therapeutic experiences.Results All three patients had symptoms of otalgia,hoarse voice and nasal bridge pit,while their entry causes at admission were anemia,renal function failure and chest pain respectively.Only one of them was insensitive to prednisone but sensitive to methotrexate.Conclusions Because PR being easily misdiagnosed,clinical doctors should improve their caution of diagnostic criteria of RP.Prednisone is the best choice for therapy,but for those insensitive ones methotrexate is also alternative.