BACKGROUND:The repair effects of bone marrow mesenchymal stem celltransplantation on brain injury were not ideal. Combined therapy with medicine and biological engineering materials is needed. OBJECTIVE:To investigate the effects of bone marrow mesenchymal stem celltransplantation and fingolimod immunosuppressants on memory function recovery in rats with brain injury. METHODS:A total of 60 healthy Sprague-Dawley rats were subjected to hydraulic shock with peak value of 253.312 5-303.975 kPa with a hydraulic head injury instrument so as to induce a model of severe hydraulic head injury. They were randomly divided into brain injury group, bone marrow mesenchymal stem celltransplantation group and fingolimod+bone marrow mesenchymal stem celltransplantation group. The Morris water maze test was tested at 21-28 days after PKH-26-labeled bone marrow mesenchymal stem celltransplantation. The PKH-26 immunofluorescence and hematoxylin-eosin staining were conducted in brain tissues at 4 weeks after brain injury. RESULTS AND CONCLUSION:At 4 weeks after transplantation, the average escape latency was gradual y decreased in each group. The average escape latency was shorter in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the bone marrow mesenchymal stem celltransplantation group (P<0.05), and significantly shorter than in the brain injury group (P<0.01). The number of times of crossing the platform and the percentage of swimming distance to total distance were higher in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the brain injury group and bone marrow mesenchymal stem celltransplantation group (P<0.05). The number of PKH-26-positive cells was significantly higher in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the brain injury group and bone marrow mesenchymal stem celltransplantation group (P<0.05). Results confirmed that bone marrow mesenchymal stem celltransplantation could apparently improve memory function of rats with severe brain injury. The combined application of fingolimod immunosuppressants has synergistic effects.

Objective To explore the related high risk factors of hyponatremia after transsphenoidal approach for pituitary adenomectomy and to direct postoperative nursing. Methods The clinical data of seventy-seven patients suffering from pituitary adenomas with the way of treatment by transsphenoidal pituitary adenomectomy from June 2014 to May 2015 were summarized retrospectively. According to the value of postoperative serum natremia, the data were divided into hyponatremia group and non-hyponatremia group. The different data between the 2 groups were compared and the high risk factors related to hyponatremia were analyzed. Results Univariate analysis showed that gender(P=0.254) and high blood pressure(P=0.742) were unrelated to postoperative hyponatremia and there was significant difference between two groups in age (P=0.038), max- diameter of tumors(P=0.004), preoperative pituitary dysfunction (P=0.013), visual deficit (P=0.000), operative procedure duration (P=0.008) and diabetes mellitus rate (P=0.023). While Logistic regression analysis showed that preoperative visual deficit (OR=0.152,P=0.004,95%CI 0.043-0.542) and preoperative pituitary dysfunction (OR=0.069, P=0.046,95%CI 0.005-0.950) were independent factors for postoperative hyponatremia. Conclusions Hyponatremia is a common complication after transsphenoidal pituitary adenomectomy. The optimal treatment can benefit the early recovery and more careful nursing should be focused on these patients who are in high risk of postoperative hyponatremia.

Extracellular vesicles (EV) miRNAs are a class of small non-coding RNAs that are encapsulated in membrane-containing vesicles from a variety of cells, and which can mediate intercellular communication and transfer of functional molecules. EV miRNAs in circulation can be served as novel molecular markers for cancer diagnosis, monitoring and prognosis, and also the most promising liquid biomarkers for clinical translation and utility currently, while optimal research strategy is crucial for identifying valuable EV miRNAs in clinical study. Moreover, EV miRNAs have recently also been implicated in cancer progression as direct regulators of metabolic reprogramming, mesenchymal-to-epithelial transition, angiogenesis, anti-cancer immune response and drug resistance in the tumor microenvironment. Study on some key EV miRNAs involving in those processes may highlight their potential utility as liquid biopsies in early diagnosis, companion diagnosis, metastasis, therapeutic control, prognosis analysis as their therapeutic potential for patients with cancer.

Objective To observe the clinical efficacy of bone-setting manipulation combined with TCM internal and external treatment for lumbar disc herniation.Methods Totally 240 cases with lumbar disc herniation were selected, and were divided into treatment group and control group according to the random number table method, with 120 cases in each group. Both groups were in bed for rest and did functional exercise. At the same time, modified Mahuang Fuzi XixinDecoction combined withDuhuo JishengDecoction were given, 1 dose per day, three times a day, orally, and were wrapped with a cloth bag dregs, adding 200 mL of vinegar, placed in lumbosacral region afer heating, 2 times a day, external treatment. The treatment group was given bone-setting manipulation combined with TCM internal and external treatment, and the control group was given traction therapy, 1 time for Monday, Wednesday and Friday, 4 weeks as a course of treatment, for continuous 2 courses. The clinical efficacy, visual analogue scale (VAS), Oswestry disability index (ODI), and serum NO content were observed.Results Compared with before the treatment, VAS and ODI score decreased significantly after the first and second courses (P<0.05); The treatment group was significantly lower than the control group at the same time after treatment (P<0.05). The total effective rate was 91.67% (110/120) in treatment group, and 83.33% (100/120) in control group, with statistical significance (Z=-2.103,P=0.036). Compared with before treatment, the NO level of the two groups significantly decreased after treatment, with statistical significance (P<0.05). The NO level in the treatment group was lower than that in the control group after treatment (t=7.843,P=0.041).Conclusion Bone-setting manipulation combined with TCM internal and external treatment have obvious efficacy for lumbar disc herniation, which may be related to down-regulating serum NO level.

Asian-pacific area,especially China,is Hepatitis B high epidemic area.Since 2011,the first generation of oral direct anti-HCV agents (DAAs) came to clinical use,the treatment of chronic hepatitis C has switched from interferon-based regimen era to DAA era.There is an increased awareness of hepatitis B (HBV) reactivation in chronic hepatitis C (CHC) patients coinfected with HBV treated with pan-oral direct-acting antivirals(DAAs).Compared with interferon-based regimen,HBV reactivation occurred earlier and more severe among patients received DAA regimen,and even fetal cases or case end up with liver transplantation was reported.Thus,association of liver diseases called to alert the occurrence of HBV reactivation among CHC patients who received DAAs regimen.It is hence important to have HBV serology screened in all CHC patients before initiation of pan-oral DAAs therapy and the usefulness of preemptive administration of effective anti-HBV nucleos(t) ide analogues in coinfected patients need to be further studied.

Objective To study the feasibility and the efficacy of total extraperitoneal repair (TEP) under two laparoscopes.Methods Clinical data of 51 patients with inguinal hernia who underwent TEP under two laparoscopes from July 2015 to March 2016 in our hospital were analyzed retrospectively.During TEP,we added a 5-mm laparoscope for monitoring and exploration in the abdominal cavity.Results All the operations of TEP under two laparoscopes were successful.The operation time lasted 42-176 min with a mean of (74 ±27) min.The intraoperative blood loss was 5-30 ml with a mean of (12.8 ±5.9) ml.The length of postoperative hospital stay was 2-7 days with a mean of (4.0 ± 1.1) days.The percentage of postoperative complications was 11.8％ (6/51),including inguinal area seroma which accounted for 2.0％ (1/51),inguinal region pain accounting for 3.9％ (2/51),and postoperative urinary retention for 5.9％ (3/51).No infection was found.The follow-up period ranged from 3 to 11 months (mean,7 months),no recurrence was observed during this period.Conclusions TEP under two laparoscopes can treat inguinal hernia precisely.It is also helpful to find compound inguinal hernia and occult inguinal hernia.

Highly active antiretroviral therapy (HAART) can effectively suppress thehuman immunodeficiency virus (HIV) replication andsignificantly reduce morbidity and mortality of HIV-infectedpatients,which howevercan't completely remove the virus,and eventually progressinto chronic viral-infection disease.Chronic HIVinfection destroys host immune system,leading to intestinal barrier damage,intestinal mucosal dysfunction,microbial translocation,and further accelerates the disease progress.The reconstruction of intestinalmicroflora balance and improvement of intestinal mucosa function areessential to reestablish the host immune system.This paper will review the current research advanceson intestinal barrier damage of HIV infection and gut-target therapy of AIDS.The aim is to provide valid evidences for further research targeting improvement of treatment strategiesandreduction of morbidity and mortality in HIV infection.

Objective To evaluate the clinical application value of flow fluorescence assay in determination of serum pepsinogenⅠ and Ⅱ.Methods The precision of flow fluorescence assay in the detection of serum pepsinogen Ⅰ and Ⅱ were evaluated. Methodology comparison were conducted between flow fluorescence assay and enzyme-linked immunosorbent assay(ELISA)kit through detecting clinical samples.The reference ranges for PGⅠ and PGⅡ/PGⅠ ratio of healthy population were established. The levels of PGⅠand PGⅡ/PGⅠ ratio were detected in serum samples of patients suffering superficial gastritis,atrophic gastritis and gastric cancer.Results The within-run and between-run coefficient of variation of PGⅠ were 4.26%-5.35% and 6.73%-7.75%,respectively.And those of PGⅡ were 5.48% - 6.42% and 8.46% -8.85%,respectively.Methodology comparison be-tween flow fluorescence assay and ELISA demonstrated good linear correlations.The linear equation wasY =0.91 1X -22.635(r=0.966,P <0.05)and Y =0.892X -0.548(r=0.980,P <0.05)for PGⅠ and PGⅡ,respectively.The lower limit of the reference range of PGⅠ and PGⅡ/PGⅠ ratio were 32.77 ng/mL and 4.1 6,respectively.The PGⅠ and PGⅠ /PGⅡ ratio of patients suf-fering atrophic gastritis and gastric cancer were statistically significantly lower than those in patients suffering superficial gastritis (P <0.05).Conclusion The flow fluorescence assay could conduct simultaneous detection of PGⅠ and PGⅡwith good methodo-logical performance and high efficiency.The determination of PGⅠ and PGⅡlevels through flow fluorescence assay could provide laboratory basis for the screening and diagnosis of atrophic gastritis and early gastric cancer.

Objective:To probe the correlation of intra-operative hyaluronic acid(HA) changes with hepatic allografts cold ischemia reperfusion injury in liver transplantation.Methods:The patients with orthotopic liver transplantation were assigned to 3 groups:① group A :liver allografts were stored in University of Wisconsin's solution(0℃) for 5-6 h before implantation;② group B :liver allografts were stored in U W's solution(0℃) for 8-9 h before implantation;③ group C :liver allografts were stored in U W's solution(0℃) for 12 h before implantation.The serum samples were taken up at 5 min,60 min,120 min,180 min after the donor liver was transplanted and reperfused to detect the concentration of HA.Results:After donor liver was transplanted and reperfused,serum HA concentration decreased immediately and gradually.HA decreased rate :group A 4.58 ?g/L?min-1,group B 4.16 ?g/L?min-1,group C 2.26 ?g/L?min-1.Serum HA decrease rate was faster in group A and B than that in group C(P

OBJECTIVE To investigate the pathogens and their drug-resistance in Fuwai Cardiovascular Hospital and provide antibiotics use suggestion for clinical treatment.METHODS The pathogens were identified by VITED 32 and analyzed by WHONET 5.4 RESULTS The pathogens mainly consisted of Gram-negative bacilli,which were highly sensitive to imipenem and meropenem except Pseudomonas aeruginosa;E.faecalis was much more sensitive to penicillin and gentamicin than E.faecium.Most coagulase-negative Staphylococcus(CNS) were resistant to oxacillin and showed low susceptibility rates to most antibiotics.No Gram-positive cooci were found to be resistant to vancomycin and teicoplanin.CONCLUSIONS To investigate the pathogens and their drug resistance is very important to prevent and control nosocomical infections.