Objective:To observe the clinical efficacy of rt-PA in the treatment of patients with acute ischemic stroke ( CIS) and hyperdense middle cerebral artery sign ( HMCAS) . Methods:Totally 107 patients with CIS and HMCAS were randomly divided into the control group (n=54) and the treatment group (n=53). The control group was treated with urokinase, while the treatment group was treated with recombinant tissue type plasminogen activator ( rt-PA) . The treatment course was 14 days, and then the clinical effi-cacy and safety were evaluated. Results:Compared with that before the treatment, the score of national institute of health stroke scale ( NIHSS) in both groups was decreased after the treatment, and the decrease in the treatment group was more notable than that in the control group with statistically significant difference (P<0. 05). The clinical effective rate of the treatment group was 88. 68%, while that of the control group was 79. 63%, and there was statistically significant difference between the groups (P<0. 05). Compared with that before the treatment, BI index in both groups was increased after the treatment, and the increase in the treatment group was more significant than that in the control group with statistically significant difference (P<0. 05). Conclusion:rt-PA thrombolytic therapy in the patients with CIS and HMCAS has remarkable clinical efficacy, which can improve patients’ life quality and is worthy of promo-tion.

Cognitive function is the advanced features of brain and neonatal cognitive function has its own development characteristics. Quantitative electroencephalogram can reflect the state of brain function directly and objectively and can be used in cognitive research. Event-related potential,induced by stimulation,is the neu-roelectrophysiologic of brain cognitive processing and time-locked to cognitive events. In recent years,research-ers have made great progress in exploring the neonatal cognitive function with the help of quantitative electroen-cephalogram and event-related potential. This review summarizes the progress of these two technologies in neo-natal brain cognitive function.

0.1mv. The forty animals were randomly divided into five groups of eight animals each: in group I normal saline 5.2ml?kg-1?h-1 was infused (control); while in group II , III , IV and V propofol was infused at a rate of 30,45, 60,75mg?kg-1?h-1 for 30 min. MAP, HR, left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), ? dp/dtmax and myocardial oxygen consumption index were recorded before and 30 min after drug administration. Blood samples was taken at the end of 30 min propofol infusion for determination of plasma propofol concentration. Myocardial tissue was taken for electron microscopic examination in some rats. The extent of infarct area was determined by TTC staining. Results The plasma propofol concentration ranged from 3.4?0.9 to (12.9 ? 2.4) ?g/ml. Propofol significantly decreased MAP, HR, LVSP,? dp/dtmax and myocardial oxygen consumption index in a dose-dependent manner. The size of myocardial infarct area ranged from 23.7% to 29.2% but there was significant difference in the size of infarct area and myocardial ultrastructure between the five groups. Conclusions Propofol inhibits hemodynamics in rats with acute myocardial infarction but has little effect on infarct size and ultrastruture of myocardial tissue.

Objective To investigate clinical effects of video assisted thoracoscopic lung volume reduction surgery(LVRS) for severe emphysema. Methods Six patients with severe emphysema underwent lung volume reduction surgery by video assisted thoracoscopy.The LVRS was performed unilateraly in 4 and bilateraly in 2 through median stemination.20%～30% of total volume of lung was resected. Results There was no operative death.All patients were followed up for 3 to 17 months.After LVRS,the mean forced expiratory volume in 1 second(FEV 1) and PaO 2 increased by 24 6% and 8 3%,respectively,Total lung capacity(TLC),residual volume(RV) and ventilatory assistance decreased by 24 6%,20 3% and 47 1% respectively Conclusions LVRS by video-assited thoracoscopy is an effective and safe technique for patients with severe emphysema.It can relieve dyspnea and improve excise tolerance and the quality of life.

Objective: To review the experience of the esophageal replacement with colon. Methods: The data from 548 patients were summarized and analyzed, including the diseases type, operations type, selection of the colon segment, colon blood supply and pathway for pulling-up of transplanted colon. Results: Postoperative complications occurred in 86 cases (15。69%), with 10 deaths (mortality rate 1。82%). The 1,3,5 years survival rate were 85。6%, 60。8% and 32。4%, respectively. All the patients in the group of benign esophageal diseases survived well with normal lives and activities, after 2～25 years of follow-up. Conclusion: There are several keys to ameliorate the results and reduce the complications rate to 15。69% and mortality rate to 1。82%. The keys are choosing of isoperistaltic transposition, ascending branch of left colica artery, retrosternal tunnel, a single-row suturing by cervical esphagocolostomy and prevention of complications (injury of recurrent laryngeal nerve, thoracic colon syndrome and esophageal pouch syndrome).

Objective To study the effect of brain-derived neurotrophic factor (BDNF) on the environmental nutrition and neural differentiation of the transplanted stem cells under hypothermia.Methods The BDNF gene mediated by liposome was transfected into 293T cell line, and ELISA assay was applied to find the peak time of BDNF expression. When BDNF was highly expressed, the supernatant was collected for establishment of SD rat models of brain injury. The rats were divided into Group A (stem cell transplantation group) and Group B (stem cell transplantation and BDNF group). Rats in both groups were under hypothermia treatment for five days. Four and eight days later ( three days from rewarming), rat brain tissues were obtained to detect the expressions of proliferating cell nuclear antigen (PCNA), nestin, neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) by immunohistochemical method and to detect the apoptosis by in situ hybridization. Finally, the nerve function scores were obtained for evaluation of the nerve function. Results The ELISA showed that the high level of BDNF expression was at 48 to 60 hours after gene transfection. PCNA and nestin were highly expressed, while NES and GFAP showed nil or low level of expression in both groups at the fourth day after hypothermia, with little apoptotic cells especially in the Group B (P ＜0.05). The expressions of PCNA and nestin were decreased, but the expressions of NSE and GFAP were increased at the third day after rewarming. The positive rate of NSE expression in the Group B was much higher and the apoptotic cells were much less compared with the Group A ( P ＜ 0. 05 ). A better nerve score was obtained in the Group B. Conclusion BDNF can enhance the survival rate of the transplanted stem cells and induce their differentiation into neurons under hypothermia.

The lumber ligaments play an important role in spinal bsomechanics. The results of three-dimensional finite element analysis showed that one of functions of lumbar ligaments is transmission of the tensile load between the lumbar vertebrae. The anterior longitudinal ligament is loaded in extension of lumbar spine and the resistance to the tensile load in flexion is provided by other ligaments. These ligaments are subject to much more tension with degsneration of the intervertebral disc so that a series of pathological changes occur. Relevant significance in clinical aspect is also discussed.

Objective To observe blood plasma matrix metalloproteinase-9 (MMP-9) of the patients with different types of carotid plaque and investigate the relationship between MMP-9 and carotid plaque vulnerability. Methods Totally 64 patients were examined by CDI, TCD, cranium CT, MRI, DSA and nerves function score (NIHSS) to judge their types of carotid plaque. Their plasma levels of MMP-9 and C-reactive protein (CRP) were detected. Results Plasma MMP-9 had direct correlation with plaque vulnerability(r=0.92,P

Object To study the chemical constituents of Dracaena cochinchinensis (Lour.) S. C. Chen. Methods The constituents were isolated on silica gel chromatography, preparative TLC, and spectral data. Results Six compounds were isolated and identified from the resin of D. cochinchinensis as: 1, 2, 4, 5-tetrachloro-3, 6-dimethoxybenzene (Ⅰ), cholest-4?-methyl-7-en-3?-ol (Ⅱ), cholest-4?-methyl-7-en-3-one (Ⅲ), hexacosane (Ⅳ), cholest-7-en-3?-ol (Ⅴ), cholest-7-en-3-one (Ⅵ). Conclusion Compounds Ⅳ-Ⅵ were isolated from D. cochinchinensis for the first time.

Objective To identify and compare melanin distribution in iris and skin tissues of rabbits from four breeds including WHBE rabbit.Methods Ferrous sulphate staining was performed for iris and skin tissue of rabbits from different breeds.Results It showed dark green staining in both iris stroma and iris pigment epithelium of WHBE rabbit, while light green staining was observed in its follicles.Conclusion The results of histologic evaluations provide some clues to the future research on mechanism of WHBE rabbit phenotype mutation.