ObjectiveAfter performed the silicone-rubber membrane implantation in rabbits, we investigated the efficacy and security of the silicone-rubber membrane implant through observed intraocular pressure, bleb and morphologic result. MethodsForty rabbits were divided into 4 groups by randomized fashiones. Each group consisted of 10 rabbits. Subscleroflapectomy and the silicone membrane implantation were operated in one eye of each rabbit. And the other eye was only performed subscleroflapectomy, which be controlled eye. The change of intraocular pressure, bleb were observed postoperatively. And every eye for histopathologic examination. ResultsThe lasted time of lower intraocular pressure and filtration bleb in implanted eyes were longer than controlled eyes in two groups. Light microscope revealed that patent drainage tract and bite site could be seen in cilicone membrafe implant eyes. The activity and regularity of fibroblast proliferation in implanted eyes and controlled eyes were similarly. The excessive expression of fibroblast proliferation was not excit ed by silicone membrane. ConclusionIt is characterized by safely, effectively and operate simply that silicone membrane implant drainage surgery act as a new drainage surgery.

Objective To observe the status and effects of the social support on rehabilitation in stroke patients.Method Personal resource Questionnaire(PRQ) were compared 198 stroke patients with 200 healthy persons,assessed neurological functional defect and Activities of Daily living(ADL) of stroke patients,when they were discharged and after two months.To observed relationship between social support and effects of rehabilitation.Result The social support scores in stroke patients were lower than those in healthy ones(P<0.01).Neurological functional defect and ADL scores were increased over the social support scores(P< 0.01).Conclusion To strengthen social support could promote rehabilitation of stroke patients.

Applications of molecular diagnostics in clinical microbiology laboratory were introduced in the article,including nucleic acid hybridization,nucleic acid amplification,DNA sequencing,gene chips and mass spectrometry.Molecular diagnostic techniques provide major tools for rapid diagnosis of infectious diseases,molecular epidemiology investigation,rapid identification of microbial pathogens and the study of the pathogenicity and antibiotics resistance.The application of these new technologies,as the complement of traditional culture methods,increases sensitivity,accuracy and diagnostic efficiency of assays.

Hypoxic ischemic encephalopathy (HIE) is one of the leading causes of neonatal morbidity and mortality worldwide, and it has a series of clinical manifestations of encephalopathy.Survivors often have different degrees of neurodevelopmental impairment.In addition to supporting symptomatic treatment, hypothermia is the safest and most effective treatment for the neuroprotection and the improvement of the prognosis of neonatal HIE.However, hypothermia cannot provide complete neuroprotection and is limited by gestational age, time, and facilities and so on.Therefore, clinicians and researchers actively seek for supplements and/or alternative therapies for neonatal HIE.It was reported that stem cell transplantation has a good application prospect in the treatment of HIE.Erythropoietin and xenon and melatonin may also play neuroprotective roles in HIE.In order to provide a better theoretical basis for clinical practices, this paper reviewed the research progress of neonatal HIE treatment.

[Summary] Laparoscopic sleeve gastrectomy ( LSG) as a sole and definitive bariatric surgery has achieved well results in terms of decreasing the body mass index ( BMI ) and percentage of excess weight loss (%EWL ) , therefore reducing the obesity-related complications , but the mechanism remains unclear .As a restrictive surgical procedure , the most important mechanism of LSG might be the reduction of the volume of the stomach resulting in a limited food intake .Therefore, to achieve the best weight loss after surgery , measuring the gastric volume is an indispensable technology .Currently, gastric capacity measurement methods include imaging measurements and intraoperative direct measurement . Imaging methods include upper gastrointestinal radiography , CT three-dimensional reconstruction , MRI and radionuclide scanning , with each having its advantages and disadvantages .In this article, the measurement methods of gastric capacity before and after LSG were reviewed .

The toxic and teratogenic effect of AlCl_3 on leghorn chick embryos were studied. Different concentrations of AlCl_3 dissolved in redistilled water were injected into the air sac of the eggs (0.2 ml per egg) on the third day of incubation. It was identified that the maximal permissible dosage was 1.708 ?mol/egg, the minimal lethal dosage was 2.25 ?mol/egg, the absolute lethal dose was 35.34 ?mol/egg, and LD_(50) was 8.09 ?mol/egg. The mortality of the embryos raised with the increase of the dose of AlCl_3. AlCl_3 also retarded the development of chick embryos and showed apparent tetratogenic effects.

Objective To study the effect of cyclosporine A drug delivery system (CsA-DDS) on the prevention of posterior capsule opacification (PCO) after experimental intraocular lens implantation in rabbit eyes. Methods Twenty healthy New Zealand white rabbits, whose left eyes and right eyes were used respectively as experiment eyes and controls, were subjected to extracapsular lens extraction and artificial lens implantation. During the operation, CsA-DDS with poly (lactideco-glycolide) as carriers or empty DDS was implanted in the capsular bag for the experimental eyes and controls respectively. After the operation, anterior chamber reaction, intraocular pressure (IOP) and CsA concentration were monitored and twelve weeks after the operation, the eyes were extracted for histopathological and morphological examinations. Results There were no differences between the two groups in conjunctival congestion,IOP change and anterior chamber reaction. PCO was less severe in the experimental eyes than in the controls. Light microscopy revealed that posterior capsular membrane in the experimental eyes was slick, with no obvious proliferation,whereas in the controls, there were lens epithelial cell proliferation and cortex regeneration of different degrees.Morphological examination with electron microscope showed that in the experimental eyes, lens epithelial cells did not function actively and apoptosis occurred, whereas in the controls, epithelial cells presented active function. No marked ultrastructural changes were found in either group. Conclusion Cs-DDS can inhibit PCO after intraocular lens implantation in rabbit eyes and does not have toxic effects on the surrounding ocular tissues. Therefore, it has a good potential for clinical use in prevention of PCO.

OBJECTIVE:To prepare aspirin gastric floating capsule and to study its in vitro drug-release characteristics.METHODS:Aspirin gastric floating capsule was prepared with aspirin as model drug,and with hydroxypropyl methylcellulose(HPMC)under different viscosity and different quantity and carbopol as main excipients.The release rate of the capsules was determined.RESULTS:When the optimal ratio between HPMC K4M and HPMC K15M was3to1,then the in vitro drug release of aspirin gastric floating capsule within0h～10h conformed to apparent zero-order kinetics with rate constant Kr at10.3%/h and release parameter at0.6173.CONCLUSIONS:The prepared capsule has a marked slow-release effect,the quantity and the viscosity of HPMC were the main factors affecting the release rate of gastric floating capsule,the in vitro drug release characteristics were associated with the joint action of bulk erosion and drug diffusion.

In recent years , respiratory tract infectious diseases and public health events caused by human adenovirus type 7(HADV-7) had been rising.Especially in Asia, the outbreaks of acute respiratory illness caused by HADV-7, accounting for more than 60%in the world, had brought great influence to the public health.In order to prevent the occurrence of HADV-7 epidemic and improve the diagnostic performance of laboratory , the research progress of HADV biological features and typing , HADV-7 epidemiological characteristics , clinical and differential diagnosis and laboratory examination methodologies were reviewed in this paper.