The cellular effects of glucocorticoids on proliferation and differentiation of a human inegakaryoblastic leukemia cell line (HIMeg) were studied in comparison with sex steroid hormones. In both liquid and methylcellulose culture systems, glucocorticoids suppressed the proliferate of HIMeg cells in a dose-dependent manner. In contrast, sex steroid hormones except progesterone had little effects on the proliferation of HIMeg cells. In liquid culture systems, morphological changes were not evident, and the percentage of cells with multilobular nuclei increased slightly from 2% to 8% after glucocorticoid treatment. Similarly, only 2% of HIMeg cells expressed glycoprotein Ⅱb/Ⅲa (GP Ⅱb/Ⅲa) antigen without hormone, whereas 30% of the cells expressed GP Ⅱb/Ⅲa with the addition of glucocorticoids. These data indicated that glucocorticoids could induce differentiation of HIMeg cells. To clarify the molecular mechanisms, glucocorticoid receptor (GR) expression was examind by Scatchard analysis, and it was found that there was a saturable, high affinity GR in HIMeg cells. Furthermore, the cellular effects of glucocorticoids on HIMeg cells could be reversed by RU486, a potent glucocorticoid antagonist. These observations suggest that the cellular effects of glucocorticoids on HIMeg cells were mediated by GR.

Objective To investigate the chemical constituents in the leaves of Dracaena cochinchinensis.Methods The compounds were separated with column chromatography and their chemical structures were identified by physicochemical and spectral method,respectively.Results Thirteen compounds were isolated from the plant. They were identified as isorhamnerin(Ⅰ),quercetin(Ⅱ),25(R)-spirostane-5-en-3?-ol(Ⅲ),gracillin(Ⅳ),25(R)-spirostane-5-en-3?,14?-diol(Ⅴ),25(R)-spirostane-5-en-3?,14?-diol-3-O-?-D-glucopyranoside(Ⅵ),25(R)-spirostane-5-en-3?,14?-diol-3-O-?-L-rhamnopyranosy(1→4)-?-D-glucopyranoside(Ⅶ),25(R)-spirostane-14?-hydroxy-4-en-3-one(Ⅷ),7?-hydroxysistosterol-3-O-?-D-glucopyranoside(Ⅸ),?-stigmasterol(Ⅹ),stigmasterol-3-O-?-D-glucopyranoside(Ⅺ),daucosterol (Ⅹ Ⅱ),and methyl ?D-glucopyranoside(Ⅹ Ⅲ).Conclusion Spirostane-type steroids are the major constituents in the leaves of D.cochinchinensis.Compounds Ⅰ-Ⅲ,Ⅴ,Ⅵ,Ⅷ,and Ⅸ are isolated from this plant for the first time.

Objective To summarize the experience on bronchiallavage to cure pulmonary atelectasis and pulmonary infection after thoracotomy.Methods 48 patients were selected and treated by bronchiallavage under monitoring,and antibiotics was used according to sputum culture and drug sensitive test.The out come was analyzed and evaluated.Results All patients of the pulmonary atelectasis were cured and those with pulmonary infection were controled.There was no serious complication.Conclusion Bronchiallavage is applied to treat pulmonary atelectasis and pulmonary infection after thoracotomy,the curative rate is significant.The appliance of antibiotics is guided by drug sensitivity.It is such a safe and feasible therapeutic tool that the bronchofibroscope is applied to cure pulmonary atelectasis and pulmonary infection after thoracotomy.

The effects of MC903, a novel 1,25-dihydroxyvitamin D3 [1,25(OH)2 D3] analogue, on proliferation and differentiation of a human megakaryoblastic leukemia cell line (HIMeg) were investigated. MC903 was found to inhibit cell proliferation and induce cell differentiation of HIMeg cells. Colony formation assay showed that MC903 (10-10 to 10-6mol/L) almost completely inhibited the colony-forming ability of HIMeg cells. Cell cycle analysis demonstrated that MC903 altered the cell cycle distribution; the fraction of cells in G0 + G1 increased while those in S and G2 + M decreased after treatment with MC903 for 4d. The effect of MC903 on proliferation and differentiation of HIMeg cells was in a dose- and time-dependent manner and was more active than that of 1, 25(OH)2, D3,. The results of the present study suggested that 1,25(OH)2 D3 as well as its novel analogue MC903 might play an important role in modulating the proliferation and differentiation of megakaryocytic lineage.

The activity of the negative regulator of cell cycle, P27, affects the normal process of cell cycle. As a type of cell cycle diseases, tumor is closely correlated with the activation of P27. It is shown that the level of P27 decreases in most kinds of tumors.By its influence on cell proliferation and apoptosis, P27 plays an important role in the occurrence and development of tumor. The activity of P27 is regulated by several pathways either at or after transcription, so various ideals which directly change the protein level of P27 or indirectly interfere the activities of elements in regulative pathways of P27 will provide many novel and efficient ways for therapies in tumors.

Thirty strains of seven Candida species from CICC(China Center of Industrial Culture Collection)were studied. The strains were differentiated by ITS1 region PCR-SSCP fingerprinting analysis and ITS2 region PCR-SSCP fingerprinting analysis. Results showed that both ITS1 region and ITS2 region were able to differentiate the seven species of Candida clearly. Contrasting the maps and effects on the identification of Candida species of ITS1 region with that of ITS2 region, result indicated that on the identification of Candida species the application of ITS2 region was better than ITS1 region.

The author reviewed some researches on teacher's work related stress in China and abroad. The present researches mainly focus on the sources of stress in teachers' work life. Some practices of work related stress in USA and Chinese special education field were discussed in the article. In the end, the author took a perspective on future research of teacher's work related stress in Chinese special education field.

Objective To study the distribution of CTX-M-ESBLs and class Ⅰ integron in enterobacter cloacae resistant to third generation cephalosporins,and discuss the relationship between CTX-M-ESBLs and class Ⅰ integron. Methods K-B test for resistance;DDST and PCR for CTX-M-(ESBLs);PCR,nested-PCR and sequence analysis for finding class Ⅰ integron carrying CTX-M-ESBLs gene cassette. Results Among 37 strains,there are 21 isolates producing CTX-M-ESBLs,20 isolates with class Ⅰ integron and 13 isolates carrying CTX-M-ESBLs as well as class Ⅰ integron.In 3 isolates: CH4,CH11 and Q1,each class Ⅰ integron haboured CTX-M-ESBLs gene cassette. Conclusions There is few report on class Ⅰ integron carrying CTX-M-ESBLs,which caused more dangers of horizontal transmission of ESBLs and was important factor to nosocomial outbreak of multiresistant clinical isolates.

0.05).The HBeAg-turned-to-negative rate was 57% in the treated group and 38.8% in the control group.The HBVDNA-turned-to-negative rate was 59.3% in the treated group and 42.4% in the control group (P

Objective To review the various imaging modalities and their appropriate applications in the preoperative evaluation of both donors and recipients in liver transplantation (LT). Methods All the relevant literatures were reviewed. Different kinds of imaging modalities have been used in potential LT donors and recipients to assess the volume, the parenchyma and the vasculature of the liver, the biliary system, and the extrahepatic region of the upper abdomen and beyond. The techniques, the imaging findings, and the merits and shortcomings of various imaging modalities were presented.Results Traditional imaging techniques are still of valuable, while CT can provide rich and accurate information. "One-stop" comprehensive MR examination is emerging as the method of choice for the preoperative evaluation LT recipients and donors. Conclusion Each imaging method has its merits and limitations. Those imaging methods which can provide accurate and comprehensive information are likely to have better application future.