Objective To evaluate the scan technique and clinical value of multi-slice spiral CT angiography(MSCTA) of coronary artery.Methods MSCTA was performed in 86 patients,and 25 of them were also investigated with selective coronary angiography(SCA).The imaging quality of MSCTA in different scanning condition were analyzed.The patency of coronary artery and other branches were examined.The distribution and extent of coronary arterial stenoses were assessed.The findings demonstrated on MSCTA were compared with those seen on SCA.Results(1)The imaging quality on MSCTA of coronary artery was significant better in patients with a heart rate below 60 beats per minute,contrast flowing rate 3.0~3.5 ml/s,volume 120~150 ml and delayed time 22~25 s;(2)in 25 cases,of 71 coronary artery and branches without stenoses examined with MSCTA,68 had identical appearance on both MSCTA and SCA.The distribution and extent of 19 coronary arterial stenoses revealed by MSCTA were correlated exactly with SCA in 29 coronary arterial stenoses.The negative and positive predicted value were 95.8% and 65.5% respectively.Sensitivity,specificity and accuracy were 86.4%,87.2% and 87.0% respectively.Conclusion MSCTA as a noninvasive examination is a valuable method in detecting diseases or variations of coronary artery.

Objective: To validate the clinical value of CT-guided curve-needle percutaneous ethanol injection (CNPEI) for celiac plexus block analgesia. Methods: Thirty-two patients with end-stage cancer, including 13 complicated with extensive retroperitoneal lymph node enlargement and fusion, were enrolled in this study. All patients complained of refractory upper abdominal pain and had received narcotic analgesics and radiotherapy, but the analgesic effect was not good. CT-guided CNPEI was therefore prescribed. Results: The effective rates of CT-guided CNPEI were 100%, 100%, 96.9%, 90.6%, 87.5%, and 84.4% immediately, and at 2 weeks, 4 weeks, 8 weeks, 12 weeks, and 16 weeks after treatment, respectively. All enlarged lymph nodes had obvious necrosis and became shrunk. Conclusion: Combined application of bilateral anterior and posterior diaphragmatic crura block and trans-lymph node block can produce good analgesic effects, and curve-needle puncture make the above technique simpler.

Objective Try to discover the safe and effective inhibitors of lymphangiogenesis. Methods Lymphatic endotheial cells from pig thoracic duct were isolated and cultured. The observation of specimens by LM and EM was made. The control group, endostatin and PF-4 experimental groups were set. Methods of the scraped line and MTT were used to examine their inhibitive effect on the lymphangiogenesis. Results The P value is

Objective To evaluate the role of bispectral index (BIS) in assessing the depth of sedation in hypogastric operation of children. Methods Forty children undergoing hypogastric operation were divided into two groups with 20 cases each by pre-school (age 3-5) (Ⅰ group) and school (age 6-12)(Ⅱ group). Sedation was solely maintained with TCI propofol. The infusion was adjusted till adequate scdation (OAA/S≤ 1 score) before sacral block. The course of propofol infusion and BIS of recovery period were closely observed. Sedation scores were assessed by special anesthetists. Results During recovery period, the mean values of BIS were gradually increased, when BIS > 76 predicted 94% sensitivity and 50% specificity in Ⅰ group, 100% sensitivity and 100% specificity in Ⅱ group. ROC area under the curve of the two groups were 0.917 ± 0.007, 0.955 ± 0.004. Conclusions BIS can predict fairly well the level of consciences during recovering periods. BIS monitoring is an effective and reliable method to guide children recovery.

Background and purpose:Exposure to estrogen is an important independent determinant of the risk of breast cancer.Moreover,chemokines and its corresponding receptors play an important role in breast cancer occurrence and development.Our study is to investigate the impact and related mechanism of estrogen on chemokines expression in breast cancer cells.Methods:Microarray was performed to analyse chemokine and corresponding receptors' mRNA expression in MCF-7 cells treated with estradiol.Real-time(RT)-PCR was applied to confirm the change of chemokine expression at different time point with different dose of estradiol in presence or absence of ICI182,780.ELISA was used to analyse the change of extracelluar secrete protein.Results:Physiological estradiol could obviously up-regulate CXCL12 mRNA expression in breast cancer cell MCF-7,which emerged from 4h after treatment and continued to 24 h.In addition,estradiol could stimulate CXCL12 protein secretion from 2h to 24h after treatment.These effects could be totally inhibited when MCF-7 was treated with ICI182,780(ER antagonist)prior to estradiol.Conclusions:Our study demonstrated that estrogen could up-regulate CXCL12 mRNA expression and its extracelluar protein secretion,which was mediated through ER signaling pathway.

Objective To evaluate the diagnostic and differential value of triphasic, especially the arterial phase, contrast enhanced CT scans for the hepatocellular adenoma, and to improve CT diagnostic accuracy. Methods Spiral CT scanning of pre and post contrast arterial phase, portal venous phase and delayed phase was performed in 5 patients with hepatocellular adenomas proved surgically and pathologically. The enhancement was administrated with venous injection of 3 ml/sec, totally 100 ml angiografin. Arterial phase of contrast enhanced scanning started 20～30 sec, portal venous phase 60～70 sec and delay scanning 3 min after the injection of contrast medium. The degrees of the enhancement of adnoma and normal tissues of liver in the three contrast enhanced phases were calculated and compared statistically. Results In plain CT, the lesions were iso density in 4 cases and slight hypodensity in 1 case, which was hardly distinguished from the normal tissue. In all 5 cases, the lesions showed marked enhancement in the arterial phase compared with normal tissue of liver (38 HU high), and there were markedly statistical difference ( t =18 94, P 0.05). The mean CT value of hepatocellular adenoma in the arterial phase was much higher than that in portal venous and delay phase, there were statistical differences between them ( F =18.39, P

Brain Injuries ; nursing ; Custodial Care ; methods ; Humans ; Infant, Newborn ; Spectroscopy, Near-Infrared

Objective To study the changes of cerebral blood flow and cerebral oxygenation during selective head cooling (SHC) in neonates with hypoxic-ischemic brain damage (HIBD). Methods Thirty-eight neonates with moderate and severe HIBD were enrolled in two groups: SHC group (n=18) and normal temperature group (n=20). The gestational age of all subjects were more than 38 weeks and Apgar score in 5 minutes after birth was less than 5 scores. Also, nerve system symptoms and electroencephalogram abnormal was found in 6 hours after birth. Neonates in SHC group had nasopharyngeal temperature maintained 34℃ and in normal trmerature guoup had rectum temperature maintained about 36℃. Seven healthy neonates were as control group. Three groups were observed 84 h. Cerebral blood flow velocity (CBFV) of middle cerebral artery (MCA) was measured with transcranial Doppler (TCD) at 6, 12, 24, 48, 72 and 84 hours after birth. Cerebral cytochrome aa3 (Cytaa3 ) was monitored continuously throughout the study using near infrared spectroscopy (NIRS). Results At 6th hours after birth, Vs [(25. 05?7. 69)cm/s]、Vd [(7. 22?3. 01) cm/s] and Vm [(15. 61?5. 97) cm/s] of MCA and △Cytaa3 in SHC group were lower, and RI (0. 81?0. 15) was higher than control group (P

Humans ; Neoadjuvant Therapy ; Stomach Neoplasms ; therapy