Objective:To explore the anti-fatigue effect of Tibet maca in mice. Methods: The mice were respectively given the powder or the alcohol extract of Tibet maca. The lactic acid concentration in blood, serum lactate dehydrogenase ( LDH) , the time of weight loading swimming and serum urea ammonia level after the exercise in the mice were detected, and the anti-fatigue effect of the powder and the alcohol extract of Tibet maca was compared. Results: After the 30-day feeding, the serum LDH activity of the mice taking the powder or alcohol extract of Tibet maca was obviously higher than that of the mice in the control group(P<0. 05), the time of weight loading swimming was significantly longer than that in the control group (P<0. 05), and the blood lactic acid concentration after the exercise was obviously lower than that in the control group (P<0. 05). Conclusion: Tibet maca can improve the time of weight loading swimming of mice, and reduce the level of serum urea ammonia after exercise and blood lactic acid concentration, sug-gesting the powder and alcohol extract of Tibet maca have obvious anti-fatigue effect.

Sera were collccted from mice immunized separately with schizonts, merozoitcs or different parasite stages, or from mice which had been recovered from Plasmodium yoelii yoelii infection. The effect of protection of cach serum against P. y. infection was assayed by passivc transfer technique. It was shown that only sera collected from mice which either had been trcated with chloroquine or had been naturally recovcred from P. y. infections can transfer certain dcgree of resistance to normal mice. The cffectivc sera delayed the appearance and the peak of parasitemia, and prolonged the survival time of the challenged mice. After these sera were fractionatcd by 50%(NH4)2SO4 precipitation, the prccipitated fraction showed similar degree of protection as the non-fractionated sera. Chloroquine-treated serum was shown to be able to inhibit the invasion of new erythrocytes by marozoite in vitro. 35S-methionine-labelcd antigens were precipitated by protective or non-protective sera and the bands were compared on SDS-PAGE and autoradiogram. Since thc antigcn bands of 245, 210, 190, 156 and 130 KD can only bc precipitated by protective sera but not by non-protectivc sera, it is possible that the protection of chloroquine-treated serum is induced by the specific antibodies against the above antigens.

Objective To explore the clinical efficacy of tension-free vaginal tape obturator inside-outside(TVT-O)in the treatment of female stress urinary incontinence.Methods In this study,we enrolled the patients with stress urinary incontinence who were treated with TVT-O(n=72)or midodrine hydrochloride(n=72).The patient self-evaluation and the Results of pad test and urodynamic test 6 and 24 months after the treatment were compared.Results These 2 kinds of treatment significantly improved the symptoms in patients with stress urinary incontinence,and in patients treated with TVT-O the clinical efficacy 24 months after the treatment was better.Conclusion TVT-O is safe and effective in treating female stress urinary incontinence,and the long-term follow-up and the improvement of urodynamic indices show it is better than simple drug treatment.

AlM:To analyze and explore the clinical efficacy of strengthening lateral tarsal strip and advancing lower eyelid retractor for the treatment of degenerative entropion with eyelid relaxation
METHODS: Reviewed from January 2011 to January 2013, 21 cases (33 eyes) of degenerative entropion eyelid relaxation were treated with operation of strengthening lateral tarsal strip and advancing lower eyelid retractor ( fornix conjunctival incision ) , the follow-up was 12-24mo, curative effect was observed.
RESULTS: All the patients were cured completely, have no recurrence or overcorrection phenomenon. The cure rate of operation was 100%, there was no complication.
CONCLUSlON: Operation of strengthening lateral tarsal strip and advancing lower eyelid retractor is a safe, reliable, efficient operation to the treatment of degenerative entropion with mode of eyelid relaxation.

Culture ; Drugs, Chinese Herbal ; Formularies as Topic ; Humans ; Language ; Medicine, Chinese Traditional ; Tablets ; Translations

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Neural Conduction ; physiology ; Paraplegia ; diagnosis ; physiopathology ; Sural Nerve ; physiopathology

AIM: To observe the effect of ischemic preconditioning (IPC) on the subsequent permanent ischemic rat retina. METHODS: A model of two-vessel occlusion (2VO) was used to give an ischemic insult to rat retina. Bilateral carotid arteries were occluded directly in the simple ischemic groups. A procedure of double 2 min ischemia- 3 min reperfusion was applied to IPC groups before their carotid arteries were occluded. Experimental control groups received the same operation except that their exposed vessels were not occluded. Other 6 normal rats served as immunohistochemical controls. Eyeballs were taken out after being subjected to 1, 3 and 7 days of ischemia. The morphometry of retina was measured by stereologic methods. The apoptosis during the retinal ischemia was detected by TUNEL. Immunohistochemistry staining was used for the localization of bcl-2 in retina. RESULTS: In IPC groups, the thickness of retina was thinner than that in simple ischemic groups. The apoptosis was less when compared with the simple ischemic groups at the same ischemic time. The apoptotic cells show only in INL and the apoptosis of RGCs was not appeared until 7 days postischemia. There was no significant difference of the numerical density of RGCs at the different time after the operation. The bcl-2 immunoreactivity was weaker than that of the simple ischemic groups too. CONCLUSION: IPC reduced the injury caused by ischemia in retina, showing a protective effect.

The study of subjcts with T2DM (n=120) and NGT (n=106) in using the screening of PGC-1? gene for SNPs by PCR-SSCP and DNA sequencing showed that four SNPs(Thr394Thr、Gly482Ser、Thr528Thr、Thr612Met) in the PGC-1? gene were found in 50 Han Chinese type 2 diabetic patients.A Gly482Ser polymorphism of PGC-1? gene was associated with type 2 diabetes.[

Objective To develop a portable, easily-used and effective fracture fixation material during emergency treatment of battle wound. Methods Based on vacuum plasticity, macromolecule particles were filled into prefabricated bag, in which the air was deflated. The bag became stiff. When it was bound with belt, it acted as fracture fixation. Results Experiments showed that this kind of splint attached closely with body without any accessorial material. The effect was good without any restriction from region or environment. Conclusion This kind of fracture fixation splint has advantages in operation time, applicable parts and fixation effects compared with other fixation material.

BACKGROUND: Tongxinluo acts on benefiting qi, promoting circulation in collateral, activating blood circulation and resolving stasis, which is well effective in treatment of ischemic cerebral vascular disease. Single-photon emission computed tomography (SPECT) provides reliable reference evidence objectively and sensitively for clinical evaluation of therapeutic effects.OBJECTIVE: SPECT and clinical neurofunctional defect score (NDS)were used to observe the therapeutic effect of tongxinluo capsule on cerebral infarction.DESIGN: Case analysis was designed.SETTING: Internal Department of Neurology of First Affiliated Hospital of Chongqing Medical University.PARTICIPANTS: Totally 22 cases of acute cerebral infarction were admitted in Internal Department of Neurology of First Affiliated Hospital of Chongqing Medical University from March 2002 and July 2003, in which 12 cases were male and 10 cases were female, aged varied from 40 to 78 years, sick duration in range from 3 hours to 3 days.METHODS: Voluntarily, 22 cases of acute cerebral infarction were randomized in tongxinluo capsule group (capsule group)(11 cases) and the control (11 cases). In the control, danshen tablet was administrated,4 tablet/time, 3 times/d. In capsule group, tongxinluo capsule group was administrated, 4 pills/time, 3 times/d. The drugs were administrated for 15 days in two groups. Before and after treatment, SPECT ROI technique was used to assay ROI average radioactive counts on the affected and healthy sides to understand local blood flowing volume of brain in two groups. Neurofunctional defect evaluation was done before and after treatment on each patient.in two groups.RESULTS: Totally 22 cases of cerebral infarction all entered result analcontrol after treatment [(49.182±5.344 5), (28.364±4.610 3) score P ＜ 0.001].the affected side of the control and treatment group before treatment was decreased. Fifteen days after treatment, the volume in original decreased flowing area of brain was improved after taking the capsule (66.536±18.676,ter administration of the capsule reflected in SPECT radionuclide, but it was not improved remarkably in the control.CONCLUSION: Tongxinluo capsule improves definitely cerebral blood flow in patients with cerebral infarction. With improvement of blood flowing in brain, the neurofunctional defect score is promoted clinically, too. It is explained that the capsule promotes the recovery of nerve function.