The scale of craniofacial intervention lies between the fields of neuro-intervention and peripheral intervention for the main purpose to investigate,diagnose and treat the disease entities originating from or supplied by the external carotid arterial system. Patients are usually refered to the oral and maxillofacial surgery,plastic surgery and otolaryngeal surgery. Craniofacial intervention includes mainly the diagnosis and treatment with adjuvant embolization of high-flow vascular diseases,intra-arterial chemotherapy of malignant tumors,embolization of epistaxis,etc. At present,there is no consensus with regard to the diagnosis and treatment of some craniofacial diseases,therefore further investigation and discussion are needed.(J Intervent Radiol,2006,15: 321-322)

0.05) from that of the cryoprecipitate, but was shorter than that in the FFP and frozen platelet. Conclusion The clinical use of the platelet cryoprecipitate suspension is superior to that of common blood components, because it includes platelets and coagulable factors.

Automobile Driving ; psychology ; Burnout, Professional ; prevention & control ; Emotions ; Humans ; Railroads

Multidrug resistance (MDR) is widely thought to be a major cause of the failure in cancer chemotherapy. The detection of MDR marker is therefore valuabe in studying the mechanisms of MDR and to predict response to chemotherapy in patient with carcinoma. Previous studies have examined MDR marker in gastric cancer, but the major part of the investigation in patients have focused on a single parameter. The purposes of this study were to detect the expression of glutathione S transferase pi(GST ?), multidrug resistance associated protein(MRP), lung resistance protein(LRP) and multidrug resistance gen 1 (MDR1) in the patients with primary gastric cancer, who had not received prior chemotherapy, and evaluate the correlation between GST ?, MRP, LRP and MDR1. The expression of GST ?, MRP, LRP and MDR1 mRNA in carcinoma tissue and the adjacent non cancerous tissue from 50 patients was examined by semiquantitative reverse transcription polymerase chain reaction (RT PCR). The positive rate of GST ? mRNA, MRP mRNA, LRP mRNA and MDR1 mRNA in gastric cancer tissue were 36.00%, 12.00%, 10.00%, and 10.00% respectively. Their expression in gastric cancer tissue was significantly higher than that in the adjacent non cancerous tissue. The overall positive rate of their expression in gastric tissue was 48.00%. The results indicated that GST ? mRNA, MRP mRNA, LRP mRNA and MDR1 mRNA are overexpressed in various degrees and no co expression exists among the studied genes in gastric cancer,and perhaps intrinsic MDR exists in 46% patients with primary gastric cancer .

Objective To investigate the influence of Norcantharidin(NCTD)on apoptosis-related gene expression of gastric cancer cell line SGC-7901.Methods The experiment was divided into control group,5-Fu group,NCTD group and 5-Fu+NCTD group.The inhibitory rate,apoptosis rate and expression of survivin,bcl-2,and caspase-3 were detected by MTT assay,flow cytometry and SABC immunohistochemical method,respectively.Results NCTD showed that the inhibitory effect on growth of SGC-7901 cells with a dose-and time-effective dependent manner.The apoptotic rate increased from(8.30?1.49)% to(20.56? 1.32)%.The expressions of survivin decreased from(86.57?4.39)% to(26.11?2.27)% and bcl-2 from(85.35? 3.25)% to(30.26?1.83)%,while caspase-3 increased from(54.49?3.07)% to(92.78?2.47)%,5-Fu had the synergistic effects with NCTD.Conclusion NCTD can inhibit the growth of SGC-7901 cell lines.5-Fu had the synergistic effects with NCTD.The mechanism might correlate with induction of cell apoptosis via effect of the expression of apoptotic-related genes such as survivin,bcl-2 and caspase-3.

Adult ; Diagnosis, Differential ; Female ; Fibroma ; metabolism ; pathology ; Fingers ; Giant Cell Tumors ; metabolism ; pathology ; Humans ; Mucin-1 ; metabolism ; Nerve Sheath Neoplasms ; metabolism ; pathology ; surgery ; Neurilemmoma ; metabolism ; pathology ; Sclerosis ; metabolism ; pathology ; Soft Tissue Neoplasms ; metabolism ; pathology ; surgery ; Tendons ; Young Adult

[Objective] To discuss the application value of spiral CT tri-dimensional reestablishment in ankle joint fracture.[Method] For 42 cases,make X-ray picture and spiral CT scanning at adem position;compare it with CT tri-dimensional reestablishment and operation.[Result] CT tri-dimensional reestablishment has key directing meaning to ankle fracture and operation style.

A new type of killer cells, named PHA-?CD3LAK, was induced by means of costimulating the peripheral blood mononuclear cells (PBMC) with anti-CD3McAb (?CD3) and rIL-2 after PHA-priming for 48 hours. Some biological characteristics of PHA-?CD3LAK, PHA-LAK and CD3AK were compared. The results showed that PHA-?CD3LAK exhibited some advantages over CD3AK and PHA-LAK in proliferation, cytotoxicity, the expression level of mIL-2R, as well as the utilizing of IL-2, suggesting the synergistic enhancing role of PHA, ?CD3 and IL-2. All three groups of effector cells were heterogeneous populations, predominantly CD3 + CD8 + T cells. The CD8 ~(+) cell percentage of PHA-?CD3LAK was higher than that of the other two groups. The application of PHA-?CD3LAK might open a new prospect to clinical therapeutic approach.

Objective To prepare an antiserum of human putative magnesium transporter protein (HPT protein) to study its biological effects on the drug resistance of gastric cancer cell by observing its expression level. Methods The cDNA encoding HPT protein was cloned and sequenced. Prokaryotic expression vector was constructed and HPT protein fused with histidine tag was expressed. Mice were immunized with the polyacrylamide gel particles containing the fusion protein, and the antisera of them were detected for antibody activity by Western blot. Using the antiserum, HPT protein expression level was assessed in different gastric cancer cell line. Results The sequence of cloned cDNA fragment was consistent with that of HPT. After BL21/pRSETB bacterium was induced with IPTG, a new protein band with a relative molecular weight of 55kD was showed on SDS PAGE profile, and further proved to be fusion protein with histidine tag by Western blot. The antiserum of immunized mice only recognized a unique protein band with a relative molecular weight of 50kD. HPT protein expression level in SGC7901/ADR was higher than in SGC7901. Conclusion The antiserum of HPT protein was successfully prepared, and HPT was found to probably take part in the gastric cancer cell MDR

ObjectiveTo study the characteristics of the relationship between injury and disease in foren-sic identification cases of cervical trauma with cervical vertebra degeneration, and to explore the prob-lems about how to identify the participation rates of injury and disease using the clinical information, forensic examination and imaging examination.MethodsSeventeen forensic identification cases of cervi-cal trauma with cervical vertebra degeneration were collected. The age distributions, injury formations, injury severities and imaging findings of these cases were analyzed and the relationship between injury and disease was evaluated comprehensively.ResultsMiddle-aged and elderly were common in 17 cases and every case was involved with intervertebral disc herniation. The main reasons of injuries were hy-perextension. The degree of injury severity and vertebra degeneration were graded according to the imag-ing findings. The participation rates of injury and disease were also calculated comprehensively.Conclu-sionThe forensic identification cases of cervical trauma with cervical vertebra degeneration should be evaluated with clinical information, forensic examination and imaging finding.