Objective To assess the post-licensure safety and immunogenicity of freeze-dried live attenuated hepatitis A vaccine(H_2 strain) in order to popularize it as an immunization program vaccine.Methods Totally 5 000 healthy infants and young children aged 18-24 months were injected with single dose of freeze-dried live attenuated hepatitis A vaccine, and the adverse events of 0-14 d after vaccination were monitored. Among them, 200 cases were selected into immunogenic subgroups, and the serum hepatitis A virus(HAV) specific IgG antibody(anti-HAV IgG) was measured 8 weeks before and after immunization.Results A total of 5 000 participants completed inoculation, and 4 999 participants completed safety observation, among which, 424 cases of adverse reactions were reported, and the incidence of solicited adverse reactions was 8. 48%. The common local solicited adverse reactions were redness, tenderness, itching, rash and swelling at the inoculation site, while the systemic adverse reactions were mainly fever and anorexia. The severity was mainly grade 1-2, and no serious adverse events related to the vaccine occurred. Among 200 immunogenic subgroups, 188 participants were included in the per protocol set(PPS), the seroconversion rate of anti-HAV IgG antibody was 98. 91% [95% confidence interval(CI): 96. 11%-99. 87%], with a geometric mean titer(GMT) of 83. 67 mIU/mL(95% CI: 77. 05-90. 85 mIU/mL).Conclusion A single dose freeze-dried live attenuated hepatitis A vaccine(H_2 strain) presents good safety and immunogenicity for infants and young children aged 18-24 months.

Angelman syndrome is a neurodevelopmental disorder characterized by developmental delay, intellectual disability, dyskinesia, speech impairment, pleasant expression, epilepsy and abnormal electroencephalogram.The early symptoms of this disease are not typical, and attention should be paid to identification.In this article, the recent advances in clinical, ubiquitin-protein ligase E3A gene, genetic characteristics, genetic counseling and the treatment strategies of Angelman syndrome will be reviewed.

Objective To explore the changes of the internal structure and research topics of oncology from 2005 to 2014,and offer reference for the development of the related studies and research policies. Methods The articles published in 203 journals in the area of oncologyfrom 2005 to 2014 included by JCR were chosen.Firstly,we cleaned the author keywords using the TDA software.After that,we standardized and categorized the keywords according to the Medical Subject Headings (MeSH)manually.In the end,we builted the co-occurrence matrixes of high-frequency words in three stages:2005—2007,2008—2010 and 2011 —2014, and drew the evolution maps and strategic diagrams of each stage to analyze the changes of the internal structure and research topics of oncology.Results While the word frequence increasing rapidly,the main keywords reduced gradually from 2005 to 2014.The traditional treatments such as chemotherapy and radiotherapy,the diagnostic and the epidemiological investigation were the core subject of oncology.With the screening of neoplasms,the research on the cell physiological phenomena and the genetic phenomena gained vigorous expan-sion.Breast neoplasms,the topic whose deep exploration also led the development of other topics,has always been the hard nut as well as the hot topic of the oncology.During this period,researches on the screening of neo-plasms and the genetic phenomena were developed rapidly.Conclusion The content of oncology will be more thoroughly with some merge,division,development,declining and perishing of the internal themes occurred as well.The boundaries among the sub-structure are becoming more and more blurred and the screening of neo-plasms and the genetic phenomena has gradually become the dominant trend in general practices.

Objective To establish immortalized bone marrow mesenchymal stem cells (BMSCs) from SD rats in vitro for further research on the characteristics and clinical application of BMSCs.Methods By using L ipo-fectamine TM 2000 -mediated gene transfection, plasmid pCMVSV40T/PUR containing the simian virus 40 large T antigene gene (SV40Tag) was transfected into BMSCs.BMSCs were screened by puromycin and then cultured on an extended scale.Their cell morphology and growth conditions were observed.Growth curve of cells was graphed.The expression of SV40Tag in transfected cells was identified by enzyme digestion method for the detection of tumor forma-tion.Results The growth rate of the experimental group of BMSCs after the 7th generation was significantly higher than that of the control group.No tumorigenicity was found in BMSCs after the 7th generation.Conclusion In vitro, the immortalized BMSCs by pCMVSV40T/PUR can provide basis on a large scale for its application in clinic and sci-entific research.

In the paper,we will deeply research sorts of ethical problems in medical genetics and their origins through a view of the human dual evolutions,and bring forward the viewpoint that any ethical problem can be originated from the conflict between human biological evolution and cultural evolution.Based on fully reasoning the relation between ethical problems in medical genetics and human evolution,the authors put forward a series of corresponding strategies to solve the ethical problems in medical genetics.

: Mild cognitive impairment (MCI) represents a transitional status between the cognition of normal aging and the fully developed clinical features of dementia. It has a high risk to progress to Alzheimer disease. Neuroimaging has become increasingly important in the clinical assessment and early diagnosis of MCI. It can also predict the course of MCI and monitor treatment effects.

Objective:To investigate the effect of shoulder girdle strengthening exercise combined with acupuncture and rehabilitation exercise on the recovery of stroke patients with hemiplegic shoulder pain.Method:Eighty-six stroke patients with hemiplegic shoulder pain who received treatment in the Department of Neurology of Ningbo No. 6 Hospital from January 2017 to December 2019 were included in this study. They were randomly assigned to undergo rehabilitation exercise and acupuncture (control group, n = 41) or shoulder girdle strengthening exercise combined with acupuncture and rehabilitation exercise (observation group, n = 45) for 8 successive weeks. The recovery of limb function, activities of daily living and shoulder pain were evaluated. Results:After intervention, the Visual Analogue Scale score in the observation group was significantly lower than that in the control group [(1.14 ± 0.48) points vs. (3.01 ± 0.62) points, t = 15.530, P < 0.05]. The Fugl-Meyer assessment score in the observation group was significantly higher than that in the control group [(42.68 ± 5.46) points vs. (30.78 ± 4.93) points, t = 10.622, P < 0.05]. The modified Barthel index in the observation group was significantly higher than that in the control group [(82.75 ± 8.93) vs. (71.48 ± 9.06), t = 5.801, all P < 0.05]. Conclusion:Shoulder girdle strengthening exercise combined with acupuncture and rehabilitation exercise can help improve patient’s ability of daily living and strengthen limb function. This treatment method is of great clinical significance and worthy of clinical popularization.

Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes,the incidence increased year by year,so the research of pathogenesis and interventions of DR is in the ascendant.The mechanism of DR mainly include glucose toxicity products and its effects on cell signaling pathways,leading to vascular disease and neuropathy.Research shows that,DR may occur with actived polyol pathway,increased,oxidative stress,the formation of advanced glycation end products (AGEs),protein kinase C pathway and vascular endothelial growth factor (VEGF)expression.db / db mice as an animal model of spontaneous type 2 diabetes,which is very similar to human type 2 diabetes,is more widely used in studies of DR.This article reviews some progresses of db / db mouse in the DR,and provide clues to further elucidate the pathogenesis of DR and development effective preventive measures.

Clinical efficacy scale of traditional Chinese medicine (TCM) should be built according to the characteristics of TCM, scientific demand, and demonstrated by practice. This paper provides some considerations of the scale.