Objective To explore the changes of the internal structure and research topics of oncology from 2005 to 2014,and offer reference for the development of the related studies and research policies. Methods The articles published in 203 journals in the area of oncologyfrom 2005 to 2014 included by JCR were chosen.Firstly,we cleaned the author keywords using the TDA software.After that,we standardized and categorized the keywords according to the Medical Subject Headings (MeSH)manually.In the end,we builted the co-occurrence matrixes of high-frequency words in three stages:2005—2007,2008—2010 and 2011 —2014, and drew the evolution maps and strategic diagrams of each stage to analyze the changes of the internal structure and research topics of oncology.Results While the word frequence increasing rapidly,the main keywords reduced gradually from 2005 to 2014.The traditional treatments such as chemotherapy and radiotherapy,the diagnostic and the epidemiological investigation were the core subject of oncology.With the screening of neoplasms,the research on the cell physiological phenomena and the genetic phenomena gained vigorous expan-sion.Breast neoplasms,the topic whose deep exploration also led the development of other topics,has always been the hard nut as well as the hot topic of the oncology.During this period,researches on the screening of neo-plasms and the genetic phenomena were developed rapidly.Conclusion The content of oncology will be more thoroughly with some merge,division,development,declining and perishing of the internal themes occurred as well.The boundaries among the sub-structure are becoming more and more blurred and the screening of neo-plasms and the genetic phenomena has gradually become the dominant trend in general practices.

In the paper,we will deeply research sorts of ethical problems in medical genetics and their origins through a view of the human dual evolutions,and bring forward the viewpoint that any ethical problem can be originated from the conflict between human biological evolution and cultural evolution.Based on fully reasoning the relation between ethical problems in medical genetics and human evolution,the authors put forward a series of corresponding strategies to solve the ethical problems in medical genetics.

Objective To establish immortalized bone marrow mesenchymal stem cells (BMSCs) from SD rats in vitro for further research on the characteristics and clinical application of BMSCs.Methods By using L ipo-fectamine TM 2000 -mediated gene transfection, plasmid pCMVSV40T/PUR containing the simian virus 40 large T antigene gene (SV40Tag) was transfected into BMSCs.BMSCs were screened by puromycin and then cultured on an extended scale.Their cell morphology and growth conditions were observed.Growth curve of cells was graphed.The expression of SV40Tag in transfected cells was identified by enzyme digestion method for the detection of tumor forma-tion.Results The growth rate of the experimental group of BMSCs after the 7th generation was significantly higher than that of the control group.No tumorigenicity was found in BMSCs after the 7th generation.Conclusion In vitro, the immortalized BMSCs by pCMVSV40T/PUR can provide basis on a large scale for its application in clinic and sci-entific research.

Angelman syndrome is a neurodevelopmental disorder characterized by developmental delay, intellectual disability, dyskinesia, speech impairment, pleasant expression, epilepsy and abnormal electroencephalogram.The early symptoms of this disease are not typical, and attention should be paid to identification.In this article, the recent advances in clinical, ubiquitin-protein ligase E3A gene, genetic characteristics, genetic counseling and the treatment strategies of Angelman syndrome will be reviewed.

Glycotechnology is a new branch of biotechnology, emerged early 1990's. In this article, the international background of glycotechnology is briefly introduced and history of glycotechnology in China is reviewed.
Biotechnology ; China ; Glycomics ; history ; History, 20th Century ; History, 21st Century

China ; Humans ; Practice Guidelines as Topic ; Rhinitis, Allergic ; diagnosis ; therapy

Child ; Glucocorticoids ; therapeutic use ; Histamine Antagonists ; therapeutic use ; Humans ; Rhinitis, Allergic, Perennial ; drug therapy

Mycoplasma pneumoniae(MP) is a common pathogen of community-acquired pneumonia in children.In recent years,with increase in the incidence of MP infection,the severe cases caused by the later pathogen are also incresing.At present the pathogenesis of severe Mycoplasma pneumoniae pneumonia(SMPP) is still not very explicit,and increased macrolide resistance of MP made a great impact on the treatment.This article reviewed the recent progress in the study of the pathogenesis,clinical manifestations,diagnosis and treatment of SMPP.

The author described the application of "people oriented" management concept to the construction of teacher's team of the university in four aspects:talent recruitment;the stability and the sustainable development of current team:the management of teacher's team,the rationalization of incentive mechanism.And the author also put forward some corresponding proposal and solution in the construction of teacher's team of the university.

Objective To analyze distribution of human papilloma virus(HPV) subtypes in women infected by HPV in Guigang area ,and to determine the dominant type in this region .Methods 21 genital HPV genotypes were screened in 13 156 women in out‐patient clinic in Guigang area from July 2013 to July 2014 by using rapid flow‐through hybridization technique of nucleic acid mole‐cules ,and high‐risk HPV detection and typing was carried out for screening cervical cancer .Results A total of 4 682 HPV‐positive women were identified and the infection rate was 35 .59% .All of 21 genetypes were checked out ,dominated by single infection .The percentages of HPV genotypes from high to low was HPV‐52 (16 .28% ) ,HPV‐16 (14 .01% ) ,HPV‐58 (8 .48% ) ,HPV8304 (7 .58% ) ,HPV‐6(6 .79% )and HPV‐11(5 .72% ) .Conclusion The dominant genetype of HPV of women in this area is HPV‐52 .