Aim To study the inhibitory effect of Oxymatrine-Baicailin compound on the secretion of hepatitis B viral antigens in HepG 2.2.2.15 cells.Methods HepG2.2.2.15 cells were cultured and treated with a series of Oxymatrine,Baicailin,or Oxymatrine-Baicailin compound respectively.The toxicity effect was determined by MTT colorimetry.Con-tents of the hepatitis surface antigen(HBsAg) and hepatitis e antigen(HBeAg) in the culture supernatants were determined by Enzyme linked immunosorbent assay.Results Oxymatrine at concentrations between 0.125 and 1 g?L~(-1) had little toxicity effect on cells,but Oxymatrine at 2 g?L~(-1) and 4 g?L~(-1) had much more toxicity effect on cells.The inhibitory effect of Oxymatrine on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1).The toxicity of Baicailin on cells increased from 0.625 to 2 g?L~(-1),especially when the dose surpassed 1 g?L~(-1).The inhibitory effect of Baicailin on HBsAg and HBeAg increased in a dose-dependent manner from 0.125 to 1 g?L~(-1),but its efficacy was inferior to Oxymatrine's efficacy.Oxymatrine-Baicailin compound had good inhibitory effect on hepatitis B viral antigens secretion,and the inhibition effect of the compound on HBeAg was superior to the effect on HBsAg.The Group C Oxymatrine-Baicailin compound had good synergism inhibition effect on hepatitis B viral antigens secretion and the inhibition rate of the specific group compound was significantly superior to that of Oxymatrine treatment alone(HBsAg:P=0.043;HBeAg: P=0.026).Conclusion Oxymatrine-Baicailin compound has good synergism effect on hepatitis B viral antigens secretion in HepG2.2.2.15 cells.

Animals ; Breast Neoplasms ; pathology ; Cell Division ; physiology ; Cells, Cultured ; Humans ; Macrophages ; cytology ; Signal Transduction ; immunology ; physiology ; Space Flight ; Tumor Cells, Cultured ; Weightlessness

Burns ; therapy ; Fluid Therapy ; Humans

ObjectiveTo study the relation of phonation and respiration in normal subjects. MethodsUsing Aerophone Ⅱ Model 6800 and Visipitch 6097, vital capacity was recorded first , then the simultaneous phonation volume, maximum sustain phonation time,mean airflow rate,sound pressure level and average pitch were recorded in order to induce the relation of phonation and respiration in normal subjects. ResultsThere was a linear correlation between phonation volume and vital capacity. Maximum sustain phonation time varied with changes of mean airflow rate and phonation volume and mean airflow rate varied little in different subjects and had no relation with average pitch and sound intensity. ConclusionMaximum phonation time, the mean airflow rate and ratio of phonation volume to vital capacity can be used as indicator in clinical application.

砄bjective:To study the effects of superlong pedicled latissimus dorsi muscle transplantation (SPLDMT) in the treatment of facial paralysis.Method: 16 cases of unilateral long standing facial paralysis were treated with SPLDMT in 1991～1998;follow up was conducted and the effects were analysed. Results:After operation satisfied movement recovery of the facial muscles was observed in 12 and improvenent in 3 of the 16 patients,no change was found in only 1 case. Conclusion: SPLDMT is effective in the treatment of unilateral long standing facial paralysis.

AlM:To analyze and explore the clinical efficacy of strengthening lateral tarsal strip and advancing lower eyelid retractor for the treatment of degenerative entropion with eyelid relaxation
METHODS: Reviewed from January 2011 to January 2013, 21 cases (33 eyes) of degenerative entropion eyelid relaxation were treated with operation of strengthening lateral tarsal strip and advancing lower eyelid retractor ( fornix conjunctival incision ) , the follow-up was 12-24mo, curative effect was observed.
RESULTS: All the patients were cured completely, have no recurrence or overcorrection phenomenon. The cure rate of operation was 100%, there was no complication.
CONCLUSlON: Operation of strengthening lateral tarsal strip and advancing lower eyelid retractor is a safe, reliable, efficient operation to the treatment of degenerative entropion with mode of eyelid relaxation.

AIM: To investigate the adsorption mechanism of pueraria on the molecular imprinting polywer(MIP) and Scatchard analysis was carried out. METHODS: Pueraria MIP was synthesized using pueraria as the template,acrylamide as the functional monomer and ethylene glycol dimethaerylate(EDMA) as the crosslinker. RESULTS: The result showed that at least two classes of binding sites were formed in the imprinted polymer.Extracting compounds from Radix Pueraria was separated by MIP. CONCLUSION: The component of the products is analyzed by HPLC.The result shows that the final products contain pueraria and the other two kinds of isoflavone.

In the paper, a microtiter plate method was used to determine the residual prekallikrein activator(PKA) activity in blood products.The result of study indicated that it would be satisfactory that the reaction mixture of PKA and prekallikrein (PK) was iucul ?? at room temperature for 30 minutes with 0.05M Tris/0.15M NaCl buffer(pH 8.0) , and then the mixture continued to be incubated at room temperature for another 15 minutes after the chromogenic substrate for kallikrein was added. The method had a good reproducibility. The PKA contents determined in IVIG agreed with that of the processing method of IVIG and the hypotensive test of rats, and were basically consistent with the result, determined by the method developed by the Finnish Red Cross Blood Transfusion Service.

Objective To investigate the effect of mytomycin C in the operation of dacryocystorhinostomy for curing lacrimal passage obstruction. Method Forty-one patients with traumatic lacrimal passage obstruction were divided into treatment group (n=21, 14 males and 7 females with an average age of 32) and control group. (n=20, 12 males and 8 females with an average age of 35). The patients in treatment group received 0.4mg/ml of mytomycin C when undergoing dacryocystorhinostomy, while the patients in control group received no medicine when undergoing the same operation. Lacrimal passage was cleaned with water after operation, and the operational effectiveness of dacryocystorhinostomy were compared between the treatment group and control group. Results Twenty-one eyes were cured in treatment group, and the cure rate reached 100%. While for the 20 eyes in control group, 17 eyes were cured, and the cure rate was 85%. There existed statistical significance between the two groups (P

Casein was hydrolysed with sulphuric acid under pressure and the hydrolysate was oxidized and nitrated by a mixture of sulphuric acid and potassium nitrate. After decolorization and neutralization to pH 4, the residue was removed by filtration.After adjusting the filtrate to pH 6.8 and supplementing it with tryp-tophane, cystine, methionine, and tyrosine, it was used as the source of amino acids ill the preparation of the medium for the microbiological assay of phenyl-aianine with Lactobacillus ardbinosus 17-5 as test microorganism.The standard curve shows that the medium is almost free from active phenyl-alanine, for blank values are of the same magnitude as those of a synthetic medium.Increasing the quantity of nitrated casein hydrolysate from 8 g. to 10 g. doesnot cause any difference in acid production. Further supplementation of themedium with fustidine, arginine, and lysine is also without effect.The results obtained by analyses of several foods with the present medium and the findings of Sauberlich and Baumann (1946), and Schweigert (1944) are in