BACKGROUND:The repair effects of bone marrow mesenchymal stem celltransplantation on brain injury were not ideal. Combined therapy with medicine and biological engineering materials is needed. OBJECTIVE:To investigate the effects of bone marrow mesenchymal stem celltransplantation and fingolimod immunosuppressants on memory function recovery in rats with brain injury. METHODS:A total of 60 healthy Sprague-Dawley rats were subjected to hydraulic shock with peak value of 253.312 5-303.975 kPa with a hydraulic head injury instrument so as to induce a model of severe hydraulic head injury. They were randomly divided into brain injury group, bone marrow mesenchymal stem celltransplantation group and fingolimod+bone marrow mesenchymal stem celltransplantation group. The Morris water maze test was tested at 21-28 days after PKH-26-labeled bone marrow mesenchymal stem celltransplantation. The PKH-26 immunofluorescence and hematoxylin-eosin staining were conducted in brain tissues at 4 weeks after brain injury. RESULTS AND CONCLUSION:At 4 weeks after transplantation, the average escape latency was gradual y decreased in each group. The average escape latency was shorter in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the bone marrow mesenchymal stem celltransplantation group (P<0.05), and significantly shorter than in the brain injury group (P<0.01). The number of times of crossing the platform and the percentage of swimming distance to total distance were higher in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the brain injury group and bone marrow mesenchymal stem celltransplantation group (P<0.05). The number of PKH-26-positive cells was significantly higher in the fingolimod+bone marrow mesenchymal stem celltransplantation group than in the brain injury group and bone marrow mesenchymal stem celltransplantation group (P<0.05). Results confirmed that bone marrow mesenchymal stem celltransplantation could apparently improve memory function of rats with severe brain injury. The combined application of fingolimod immunosuppressants has synergistic effects.

Objective To explore the related high risk factors of hyponatremia after transsphenoidal approach for pituitary adenomectomy and to direct postoperative nursing. Methods The clinical data of seventy-seven patients suffering from pituitary adenomas with the way of treatment by transsphenoidal pituitary adenomectomy from June 2014 to May 2015 were summarized retrospectively. According to the value of postoperative serum natremia, the data were divided into hyponatremia group and non-hyponatremia group. The different data between the 2 groups were compared and the high risk factors related to hyponatremia were analyzed. Results Univariate analysis showed that gender(P=0.254) and high blood pressure(P=0.742) were unrelated to postoperative hyponatremia and there was significant difference between two groups in age (P=0.038), max- diameter of tumors(P=0.004), preoperative pituitary dysfunction (P=0.013), visual deficit (P=0.000), operative procedure duration (P=0.008) and diabetes mellitus rate (P=0.023). While Logistic regression analysis showed that preoperative visual deficit (OR=0.152,P=0.004,95%CI 0.043-0.542) and preoperative pituitary dysfunction (OR=0.069, P=0.046,95%CI 0.005-0.950) were independent factors for postoperative hyponatremia. Conclusions Hyponatremia is a common complication after transsphenoidal pituitary adenomectomy. The optimal treatment can benefit the early recovery and more careful nursing should be focused on these patients who are in high risk of postoperative hyponatremia.

Extracellular vesicles (EV) miRNAs are a class of small non-coding RNAs that are encapsulated in membrane-containing vesicles from a variety of cells, and which can mediate intercellular communication and transfer of functional molecules. EV miRNAs in circulation can be served as novel molecular markers for cancer diagnosis, monitoring and prognosis, and also the most promising liquid biomarkers for clinical translation and utility currently, while optimal research strategy is crucial for identifying valuable EV miRNAs in clinical study. Moreover, EV miRNAs have recently also been implicated in cancer progression as direct regulators of metabolic reprogramming, mesenchymal-to-epithelial transition, angiogenesis, anti-cancer immune response and drug resistance in the tumor microenvironment. Study on some key EV miRNAs involving in those processes may highlight their potential utility as liquid biopsies in early diagnosis, companion diagnosis, metastasis, therapeutic control, prognosis analysis as their therapeutic potential for patients with cancer.

Accidents, Occupational ; mortality ; Animals ; Cause of Death ; Humans ; Male ; Nitriles ; poisoning ; Rats

The article sort out the main planting information of Chinese medicinal materials in Tanchang county from 2012 to 2014, through doing a questionnaire survey, setting technical training and communicating with people who plant, process, and sell the Chinese medicinal materials in the main producing area in 25 villages and towns of Tanchang county. By analyzing the results, the author pointed out the current situation of Chinese medicinal material planting, processing, trading and also the existing problems, and gave a proposal and strategy to the industrial development of Chinese medicinal materials, therefore, set an example to the development of the county territory.
China ; Data Collection ; Drugs, Chinese Herbal ; Plants, Medicinal ; growth & development

Integrin-linked kinase (ILK) is a widely expressed protein kinase that relate to cellular growth and differentiation. It is most abundant in the heart. Recently, many researches revealed that ILK is highly relevant to cardiac response to biomechanical stresses. Also, ILK plays important roles in regulation of the occurrence and development of cardiac hypertrophy, dilated cardiomyopathy, viral myocarditis and myocardial senescence via correlation to several classical signal transduction pathway. Meanwhile, ILK functions in protection after myocardial infarction. This article will try to summarize the effects and relevant mechanism of ILK in above-mentioned aspects, overall reveals the roles of ILK in heart and its potential clinical significance.

Objective To summarize the experience on bronchiallavage to cure pulmonary atelectasis and pulmonary infection after thoracotomy.Methods 48 patients were selected and treated by bronchiallavage under monitoring,and antibiotics was used according to sputum culture and drug sensitive test.The out come was analyzed and evaluated.Results All patients of the pulmonary atelectasis were cured and those with pulmonary infection were controled.There was no serious complication.Conclusion Bronchiallavage is applied to treat pulmonary atelectasis and pulmonary infection after thoracotomy,the curative rate is significant.The appliance of antibiotics is guided by drug sensitivity.It is such a safe and feasible therapeutic tool that the bronchofibroscope is applied to cure pulmonary atelectasis and pulmonary infection after thoracotomy.

OBJECTIVE To investigate the pathogens and their drug-resistance in Fuwai Cardiovascular Hospital and provide antibiotics use suggestion for clinical treatment.METHODS The pathogens were identified by VITED 32 and analyzed by WHONET 5.4 RESULTS The pathogens mainly consisted of Gram-negative bacilli,which were highly sensitive to imipenem and meropenem except Pseudomonas aeruginosa;E.faecalis was much more sensitive to penicillin and gentamicin than E.faecium.Most coagulase-negative Staphylococcus(CNS) were resistant to oxacillin and showed low susceptibility rates to most antibiotics.No Gram-positive cooci were found to be resistant to vancomycin and teicoplanin.CONCLUSIONS To investigate the pathogens and their drug resistance is very important to prevent and control nosocomical infections.

Objective To study the possibility of ultr asonic examination in evaluation of cleft palate repair. Methods The length, open-close movement of soft palate and velopharyngeal insufficiency were observed by ultrasonography in submaxillary region pre-operation and post-operation in 7 patients. Results After operation- the length of soft palate was longer than that before operation, the mean extended length was 13.7- mm; the movement of soft palate was increased; the velopharyngeal insufficiency was improved. Conclusions The observation of soft palate structure pre-operation and post-operation by ultrasonic examination is an objective index for evaluating the cleft palate repair.

Objective To evaluate the effect of esmolol on refractory veatricular fibrillation just after aortic declamp during cardiopulmonary bypass.Methods 40 patients undergoing valve replacement occurring refractory ventricular fibrillation after release of aortic cross-clamp was randomly given esmolol (prepared to 10 mg/ml) 1 mg/kg(group esmolol,n =20),or Lidocaine (prepared to 10 mg/ml) 1 mg/kg(group control,n=20),the endocardial electric defibrillation was continued after 2min.If the fibrillation still can not be reversed after another two times defribrillation,the routine clinical method would beused.The time of aortic cross-clamp,rectal temperature,MAP,and the value of serum Lactic acid,potassium,and PH were recorded.After intervention,the times of defibrillation,heart rate and rhythm 5 min after reversal were recorded.At the end of CPB,the CPB time and the dosage of positive inotropic drugs were also recorded.Results The success rate of defibrillation was higer in group esmolol than control group(P ＜0.05).Heart rate after reversal in group esmolol were slower than that in control group(P ＜ 0.05).The CPB time of control group was longer than group esmolol (P ＜ 0.05),and the dosage of positiveinotropic drugs was significantly higher in control group(P ＜ 0.01).Conclusion When occurring refractory ventricular fibrillation just after aortic declamp during CPB,Using esomolol by way of intravenous infusion can apparently reduce the frequency of defibrillation,and improve the rate of rewersal.It can also be favorable to cardiac function,and decrease the dependency of positive inotropic drugs,and shorten the time of CPB.