Orthotopic liver transplantation(OLT) has been performed for 42 years.Survival rates have climbed to as high as 88% at 1-year and operative mortality rates have dropped to as low as 0%.Liver resection should still be considered the first-line treatment for patients with hepatocellular carcinoma(HCC) in non-cirrhotic and resectable livers.Liver transplantation must be the preferred approach for cirrhotic livers.OLT for HCC at a more advanced stage is not indicated.Combination therapy with hepatitis B immune globulin(HBIG) and lamivudine for hepatitis B virus(HBV) patients before and after OLT has been reported a survival benefit as compared to non-HBV recipients.Liver transplantation should not be advocated at the hospitals without primary working conditions.

Objective To establish malignant biliary obstruction model on SD rats and to study the effects of Danshen injection on the growth of hepatocellular carcinoma, morphological change and the expression of proliferating cell nuclear antigen (PCNA) in carcinoma and peri-carcinoma tissues. Methods Malignant biliary obstruction model on SD rats was made by vaccination of transplanted tumor in the liver nearby the portal hepatis with Walker-256 hepatocarcinoma cell line which infiltrating and metastasizing to proximal common bile duct causing an obstruction. The SD rats were divided into four groups: those treated with 0.9% N S (n=12), with Inosine+VC (n=20), with Danshen (n=20) and with 5-FU (n=20), respectively. Results The growth and expression of PCNA in carcinoma, peri-carcinoma adjacent lobe tissues were significantly downgraded after treatment by Danshen injection compared with the rats treated by 0.9% N S and Inosine combined with Vitamine C and the infiltration was increased. The expression of PCNA in carcinoma, peri-carcinoma adjacent lobe tissues were downgraded compared with that of the rats treated by 5-FU while growth of hepatocarcinoma was slightly downgraded after treatment with Danshen injection. Conclusion Danshen shows protective effects on morphological change in SD rat model of malignant biliary obstruction. It can also inhibit the growth of hepatocarcinoma through inhibiting the proliferation of hepatocarcinoma cells and increasing reversion of hepatocarcinoma cells.

Objective To investigate the role of FHIT protein and cPKC? in invasion and metastasis in non-small cell lung cancer(NSCLC).Methods The expression of FHIT protein and cPKC? in 41 specimens of NSCLC and 11 cases benign lung tissues were examined by immunohistochemical technique.Results The positive rate of FHIT protein and cPKC? in NSCLC tissues were 48.8% and 53.7% respectively.The positive rate of FHIT protein and cPKC? in benign lung tissues were 90.9% and 18.2% respectively.They were significantly different(P

Objective To investigate survivin as an anticancer therapeutic target by use of shepherdin [79-87],a novel peptide carrying the survivin sequence from Lys-79 through Leu-87,we constructed an recombinant vector containing fusion gene NT4-Ant-Shepherdin [79-87].Methods The gene of Ant-Shepherdin [79-87] was obtained by PCR and T-vector method.After cloned and digested with restricted enzyme,Ant-shepherdin [79-87] was inserted in PBV220NT4 vector.The recombinant vector was transformed into the competent cell,E.coli DH5?.The fusion gene of NT4-Ant-Shepherdin [79-87] was identified by agarose gel electrophoresis (AGE).Results DNA sequencing results verified that the sequence of Ant-Shepherdin [79-87] was consistent with what we had designed.After transformed E.coli DH5?,a fragment of 321 bp was confirmed.Conclusion The recombinant vector containing fusion gene NT4-Ant-Shepherdin [79-87] was successfully constructed in this experiment by molecular biology techniques,which provides the basis of further research of survivin for cancer gene therapy.

Objective To make a comparative analysis of conventional smear and liquid-based cytology method to diagnose lung cancer in sensitivity and false-negative,and evaluate the application value of liquid-based cytology in sputum samples.Methods We collected 287 sputum samples through clinical contrast conventional smear with liquid-based cytology method.The diagnosis was made by the same pathology panel in double blind way.We compared the two methods to diagnose lung cancer in sensitivity and false-negative.Results Liquid-based cytology method detected 62 cases of lung cancer,and conventional smear detected 49 cases of lung cancer,with the sensitivity rate being 21.6% and 17.1%,respectively.Combination of the two methods made the sensitivity rate 24.0%.The false-negative rate was 38.6% for liquid-based cytology method and 48.5% for conventional smear which did not differ significantly.By combining the two methods,the false-negative rate was 24.0%.There was no significant difference between liquid-based cytology method and conventional smear,and between liquid-based cytology method and the two-method combination in sensitivity and false-negative.But there was a significant difference between the two-method combination and conventional smear.Conclusion Through sputum sample detection of lung cancer cell,liquid-based cytology method is better than conventional smear,the former having obvious superiority;combining the two methods can detect more lung cancers.Therefore,it is a superior screening test in detecting lung cancer and worthy of clinical popularization.

Acute Disease ; Dexamethasone ; therapeutic use ; Glucocorticoids ; therapeutic use ; Humans ; Pancreatitis ; complications ; therapy ; Respiratory Distress Syndrome, Adult ; etiology ; prevention & control ; Systemic Inflammatory Response Syndrome ; etiology ; prevention & control