Objective To observe the effects of early rehabilitation training on endothelial progenitor cells (EPCs),stromal cell-derived factor alpha 1 (SDF-lα) and motor function recovery in hemiplegic patients with acute cerebral infarction.Methods Fifty hemiplegic patients after a first acute cerebral infarction were randomly divided into a control group (n =25) and an experimental group (n =25).Both groups of patients were given routine drug therapy,while the experimental group also received early rehabilitation (within 48 hours of onset).EPCs and SDF-1α levels in peripheral blood were measured before and after one week of treatment,and Fugl-Meyer assessments and modified Barthel index scoring were conducted at admission and after three months of treatment.Results There was no difference between the 2 groups before the treatment.After a week of treatment,however,the EPC and SDF-1α values of both groups had increased,with those of the experimental group increasing significantly more than in the control group.Spearman rank correlation analysis showed that the increase in EPCs was positively correlated with the SDF-1α increment in the first week.After 3 months of treatment,the average FMA and MBI results of the experimental group were significantly better than those of the control group.Conclusion Early rehabilitation training can help to further improve the recovery of motor function for hemiplegic patients after acute cerebral infarction.This may be related to its effect in upregulating the expression of SDF-1α,thus contributing to the mobilization of EPCs in the bone marrow.

Objective To explore the effect of electroacupuncture on CD 34 +VEGFR2 +endothelial progenitor cell (EPC)-derived vessels and stromal cell-derived factor-1α(SDF-1α)/CXCR4, and study its mechanism of promoting an-giogenesis in hippocampus after focal cerebral ischemia /reperfusion .Methods A total of 180 healthy male adult Sprague Dawley (SD) rats were randomly divided into sham operation (sham) group, model (I/R) group, electroacupuncture (I/RE) group, I/RE plus AMD3100 (A specific antagonist of CXCR4) group (I/REA) and AMD3100 (I/RA) group. The rats received filament occlusion of the right middle cerebral artery for 2 hours followed by reperfusion .Electroacupunc-ture was applied to “Baihui” (GV20)/“Siguan” (Hegu LI 4/Taichong LR 3) acupoints for 30 min, once a day.The mR-NA expression of SDF-1αand CXCR4 were detected by reverse transcription-polymerase chain reaction ( RT-PCR) .Double immunofluorescence was used to stain CD 34 +VEGFR2 +EPC-derived vessels.Results Compared with the sham group, the mRNA expressions of SDF-1αand CXCR4 were significantly upregulated in I/R and I/RE group ( P<0.05 ) , but that in I/RE group was more significantly increased than I/R group(P<0.05).In addition, the mRNA expression of SDF-1αand CXCR4 were highly increased on day 1 in the I/REA group than that of I/RE group, but decreased than that of I/RE group on day 7 after reperfusion (P<0.01).CD34 +VEGFR2 +EPCs-derived vessels were obviously increased on 3d and 7d in the I/RE group compared with that of the I/R group, and significantly decreased on 7d in the I/REA group compared with that of the I/RE group ( P<0.01) .Conclusions Electroacupuncture can effectively promote an-giogenesis through upregulating the expression of SDF-1αand CXCR4 in rat ischemic hippocampus after focal cerebral is-chemia/reperfusion.

Objective:To explore the symptom perception and coping experiences of patients with low anterior resection syndrome (LARS) after anus-preserving surgery for rectal cancer.Methods:This study adopted the phenomenological research method in qualitative research. From October 2022 to January 2023, purposive sampling was used to select 15 postoperative LARS patients with rectal cancer who underwent anus-preserving surgery and underwent outpatient follow-up or hospitalization at Henan Cancer Hospital as the research subject for semi-structured in-depth interviews. Colaizzi 7-step analysis method was used to analyze, summarize, and extract themes from interview data.Results:Four themes were extracted, including attitudes and emotional responses towards symptoms, daily activities influenced by symptoms, coping status with symptom management strategies, and the need for multidimensional support for symptoms.Conclusions:LARS patients after anus-preserving surgery for rectal cancer have many discomfort symptoms and are eager to receive support and assistance. Medical and nursing staff should pay attention to the complexity of patient symptom management and promptly conduct effective symptom assessment and intervention, providing patient-centered comprehensive supportive nursing, and improving their quality of life.

Objective : To explore the biocompatibility of Polydimethylsiloxane/Chlorhexidine gluconate ( PDMS/ CHG) composite coating on titanium surface . Methods : CHG with different concentrations were covalently grafted to the surface of titanium through PDMS and divided into 5 groups: Group Ti: blank control group; Group P:PDMS coating group; Group C1:treated with PDMS and 0.2 mg/ml CHG;Group C2:treated with PDMS and 0.4 mg/ml CHG;Group C3:treated with PDMS and 0.8 mg/ml CHG;the surface morphology of titanium was observed with scanning electron microscope(SEM),and rat gingival fibroblasts(RGFs) were inoculated to the sample surface co-cultivation.Confocal laser scanning microscope(CLSM)was used to observe the cells, on the surface of titanium, morphology and the state of the cell after FITC-labeled phalloidin and AO/EB staining, besides, CCK-8 was used to detect cells proliferation. Results: It could be seen by SEM that films had been formed on the surface of titanium sheets in each experimental group.Combined with elemental analysis, the films on the surfaces of C2 and C3 groups were uniform and dense; CLSM showed that the cell morphology of each experimental group was consistent with that of the titanium group, with better extension, and the results of AO/EB staining showed that there was a lot of green fluorescence in each group, and the cells were in good shape.CCK-8 results showed that there was no difference between each group(P>0.05). Conclusion Titanium surface combined with concentration of no more than 0.8 mg/ml PDMS/CHG coating has good biocompatibility.

Objective : To explore the antibacterial coating of polydimethylsiloxane⁃chlorhexidine gluconate (PDMS⁃CHXG) constructed on the smooth titanium surface and antibacterial properties for Porphyromonas gingivalis of CHXG solution with different concentrations. Methods : The titanium was polished to 7 000 mesh to mirror shape of abutment, cleaned and dried, then treated with alkalization. The samples were randomly divided into 5 groups: blank control group (C), test groups: grafted CHXG concentration of 0 (T0 ), 0. 4 (T1 ), 0. 8 (T2 ), 1. 6 mg/ml (T3 ) . The surface structural changes were observed by cold field emission scanning electron microscope (CFESEM), and the component elements of coating were analyzed semi⁃quantitatively. The antibacterial properties of the coating were evaluated by antibacterial zone, live/dead bacteria staining and crystal violet experiments. Results: A dense film was formed on the surface of titanium under the CFESEM compared with C group. The content of Cl element increased with the increase of CHXG concentration. There was no inhibition zone around the samples in C and T0 groups, but it was found in T1 , T2 and T3 groups. Live/dead bacteria staining showed no viable bacteria in the T2 and T3 groups. The results of crystal violet staining showed that T1 , T2 and T3 groups were statistically different from C and T0 groups, but the difference between the groups T2 and T3 was not statistically significant. Conclusion The antibacterial coating of PDMS⁃CHXG is constructed successfully. The PDMS⁃CHXG coating displays an exceptional antibacterial property when the concentration of CHXG reaches 0. 8 mg/ml.