1.Integrin-linked kinase and diabetic nephropathy
Journal of Medical Postgraduates 2004;0(01):-
Cellular functions like proliferation,differentiation,migration,morphogenesis and apoptosis are modulated by the extracellular matrix.Integrins are the prototypic heterodimeric transmembrane matrix receptors with competing affinities for individual extracellular matrix ligands.The intracellular integrin domain clusters cytoplasmic proteins into focal adhesion plaques for bidirectional(outside-in and inside-out) signaling.Integrin-linked kinase(ILK) is an intracellular serine/threonine protein kinase that interacts with the cytoplasmic domains of ?-integrins.ILK organizes the connections of the extracellular matrix via integrins to the cytoskeleton and is involved in adhesion plaque signaling,and it plays a crucial role in the pathogenesis of chronic renal fibrosis.High glucose induces the upregulation of the synthesis and activity of ILK in cultivated mouse podocytes and mesangial cells.The ILK protein level is significantly increased in diabetic glomeruli.ILK activity is likely to provide the basis for an effective therapeutic method for diabetic nephropathy.This review gives an introduction of ILK structure and function,followed by a summary of our current understanding of ILK in diabetic nephropathy with a special focus on glomerular cell-matrix interaction.
2.Reseach Progress on Molecular Detection Technology of Influenza Virus
Journal of Modern Laboratory Medicine 2016;(1):68-70
Influenza viruses are global epidemic and diversely difficult to distinguish,which threaten human’s survival and development very much.In recentyears,the frequent outbreaks of influenza prompt the rapid development of Influenza virus detection.Compare with the traditional isolated culture and immunological detection,molecular diagnostic technology is of high detection speed,high sensitivity and specificity,that gradually play an important role in the current Influenza virus de-tection.In order to provide a theoretical basis for the rapid diagnosis of Influenza virus in the clinic,the article summarize the update progress of molecular biology and diagnostic techniques of Influenza viruses.
3.Effects of short-term insulin pump treatment on pancreatic ?-cells in newly diagnosed type 2 diabetes mellitus
Fuzhen LIU ; Wenpu CHEN ; Yu CHEN
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
Objective To explore the effects of insulin pump on pancreatic ?-cells in type 2 diabetes mellitus accompanied with hyperglycaemia.Methods We treated 46 cases of newly diagnosed type 2 diabetes mellitus with fasting blood glucose≥10.0 mmol/L by insulin pump for 2 weeks.Their blood sugar and insulin were determined before and after treatment by oral glucose tolerance test and insulin releasing test.We then calculated the function index,morning secretion of pancreatic ?-cells and insulin resistance index.The patients were followed up for 6 months and the blood glucose was monitored.Results After treatment,insulin,function index and the morning secretion were increased significantly,while the blood sugar and resistance index were decreased.In all the patients,16 could maintain ideal blood glucose only by alimentary control and exercise.Conclusion With the insulin pump in a short term,patients with type 2 diabetes mellitus can rapidly control blood glucose,obviously improve pancreatic ?-cells,and reduce insulin resistance.
7.Epidemiology of serum lipid abnormality in patients with coronary artery disease
Hao LIU ; Jinming YU ; Fang CHEN
Chinese Journal of Practical Internal Medicine 2000;0(12):-
Objective To investigate the lipidemia of patients with coronary artery disease(CAD)in China.Methods The survey engaged 52 centers in 7 cities in China recruiting patients with coronary artery disease in a hospital-based setting.Each center was asked to recruit all hospitalized patients with CHD from 1-Jun-2005 to 31-Sep-2005.The study population consisted of 3513 patients with CAD.Results Of the 3513 patients,the prevalence of the lipidemia was 79.7%.And the prevalence of the lipidemia in
8.The influence of smoking upon level of serum interleukin and TNF-? in patients with bronchial asthma
Changli YU ; Sa LIU ; Zhiquan CHEN
Chinese Journal of Practical Internal Medicine 2006;0(13):-
Objective To explore the influence of smoking upon the level of the serum interleukin and TNF-? in patients with bronchial asthma.Methods Sixty-five patients with bronchial asthma,grade 3 or 4 as asthma group and 50 healthy examinees as control group were selected.According to the smoking history,each group was divided into the smoking subgroup and the non-smoking subgroup.For each group,the level of cytokines in serum were detected,and at the same time,their smoking history and general condition were collected.The differences between the groups were compared and the relationship of all indexes was analyzed.Results Compared with the control group,the level of IL-2、IL-6、IL-8 and TNF-? of the asthma group was significantly higher(P0.05).The level of IL-2、IL-8 and TNF-? of the smoking asthma subgroup was significantly higher than the smoking control subgroup(P0.05).Conclusion The influence of smoking upon the level of cytokines of asthma patients is significant.The level of IL-8、TNF-? in serum of a smoking asthma patient increases.The level of five cytokines in serum of 65 asthma patients in our research is related to patient's condition severity.
9.Dynamic changes and their clinical significance of plasma ET, CGRP and ANP in elderly patients with acute cerebral infarction
Jing YU ; Xueyuan LIU ; Yujuan CHEN
Journal of Clinical Neurology 1997;0(06):-
Objective To determine the dynamic changes of plasma endothelin (ET), calcitonin gene related peptide (CGRP) and atial natriuretic peptide (ANP) in elderly patients with acute cerebral infarction and explore the pathological role of ET, CGRP and ANP in the development of acute cerebral infarction.Methods The concentrations of ET, CGRP and ANP in plasma were determined with radioimmunoassay in 78 elderly patients with acute cerebral infarction and 60 normal controls.Results (1)The levels of ET and ANP in patient group were significantly higher than those in control group ( P3 cm) and small area infarct group (1.5~3.0 cm) were significantly higher (all P
10.Diagnosis and treatment of radial nerve injury following internal fixation for humeral shaft fracture
Zhigang LIU ; Jiaao YU ; Lei CHEN
Chinese Journal of Orthopaedic Trauma 2004;0(12):-
Objective To explore the etiology and treatmen t of radial nerve injury following in ternal fixa-tion for humeral shaft fracture.Methods 15patients were treated in our department from 1997to 2003for radial nerve injury following steal-plate internal fixation.They were selected for this study and followed up from6to 18months.Their injury cause,clinica l manifestation,methods of treatme nt,diagnosis and prognosis were retrospec-tively analyzed.Results The symptoms of nerve injury disappeared in all patients.Their muscle strength of wrist and digit extension returned to grad e V.The electromyogram showed that t heir nerve conduction velocity and l atent period returned to normal.Conclusions Excessive traction,careless manip ulation and steel plate compression are the main causes for iatrogenic radia l nerve injury.The nerve function can be completely recovered with early diag-nosis and early neurolysis.[