OBJECTIVE:To establish an HPLC method to determine the content of main component in idrocilamide ointment. METHODS: The samples were separated on HP-ODS column with column temperature maintained at room temperature. The mobile phase consisted of methanol-water (65∶35) with a flow rate of 1.0 mL?min-1. The detection wavelength was set at 276 nm and the injection volume was 20 ?L. RESULTS: The linear range of idrocilamide was 0.05～4 ?g?mL-1,the average recovery rate was 99.97% (RSD=0.80%). CONCLUSION: This method is sensitive,accurate and applicable for the determination of the content of main component in idrocilamide ointment.

AIM:To construct human interleukin-18 receptor ?(IL-18R?) and interleukin-18 receptor ?(IL-18R?) eukaryotic expression vectors named pcDNA3.1-zeocin/IL-18R? and pcDNA3.0/IL-18R? respectively,and then stably transfect pcDNA3.0/IL-18R? into 293 cells in order to establish the cell line stably expressing IL-18R? and cell model which responds IL-18 signal transduction in vitro. METHODS:The experiment was performed at the laboratory of Department of Immunology,Peking University Health Science Center from June 2006 to May 2007. DNA encoding IL-18R? or IL-18R? was amplified and cloned into pcDNA3.1-zeocin or pcDNA3.0 vector,respectively. The recombinant of pcDNA3.0/IL-18R? was transfectd into 293 cells. After screening culture by G418,stable transfected cell line was established and the expression of IL-18R? was identified by Western-blot assay. The stable cell line with the combinant eukaryotic expression vector pcDNA3.1-zeocin /IL-18R? to establish the IL-18 signaling pathway,which was detected by NF-?B-dependent Luciferase. RESULTS:The eukaryotic expression vectors of IL-18R? and IL-18R? were constructed successfully. The gene of IL-18R? stably expressed in 293 cells and cell model of IL-18 signaling transduction in vitro could be established in these stable 293 cell lines. CONCLUSION:The establishment of NF-?B-activated cell model in vitro which responds to IL-18 can provide solid foundation for further experimental studies on IL-18 signaling transduction pathway.

Combined with the features of clinical noviciate teaching of orthopaedics and our teaching practice,we discuss how to improve the quality of clinical noviciate teaching of orthopaedics and strengthen the cultivation of medicos' capability and diathesis,thus correctly guiding medicos to finish their interim study commendably and change their roles from students to doctors.

Objective To explore the blood level lead and the related high-risk factors in Uigur and Han children in Urumqi by answer tree. Methods Cluster sampling was used to investigate blood lead level of 988 Uigur and Han children aged 6-10 years,the related high-risk factors were investigated by using a standardized questionnaire,the related high-risk of blood lead level was analyzed by answer tree. Results The geometric mean of blood lead concentration of children was 54.46 ?g/L,in 23.28% of the children,the blood lead concentration was more than 100 ?g/L . No significant blood lead level difference was seen between Uigur and Han children. Such high-risk factors as parents occupation,bad habits and housing condition were associated with the blood lead level. Conclusion The blood lead level and lead poisoning rate of children in Urumqi are higher,many factors may influence the blood lead level of children in Urumqi.

The nutritional status in early life have been gradually recognized that it can change the status of development and metabolism of adults.Epidemiological evidence and animal model study have found that low birth weight is the risk factors of adult metabolic syndrome and cardiovascular disease.Insulin resistance is a common pathophysiological basis.Renin-angiotensin system and insulin signaling systems interact to promote the development of insulin resistance.

0.05).Conclusions The serum MMP-9 can be tested as an objective index in appraisal the severity of the left to right shunt.The increased level of MMP-9 may be one of important mechanism for developing heart failure process in children with left to right shunt CHD.Monitor the level of MMP-9 will help us find adverse myocardial remodeling earlier and begin treatment immediately to prevent heart failure developing.

Objective To survey the cognition of women to labor analgesia in Shanghai.Methods One hundred randomized pregnant women filled in the questionnaires at obstetric outpatient department in our hospital.One hundred women took part in the survey after labor at maternity ward,ninety of whom underwent patient-controlled epidural analgesia with 0.1% ropivacaine plus 0.002% fentanyl,and the rest 10 inhaled 50% nitrous oxide mixed with 50% oxygen.Results Before labor analgesia,98% pregnant women thought that the labor was painful;42% were nervous and frightened about the labor pain;90% knew nothing about labor analgesia;63% had no idea about the influences of labor analgesia on infants;and 14% worried that analgesia would have side effects on infants.After labor analgesia,99% women were satisfied with its pain relief effect;100% considered that labor analgesia was necessary;and 20% worried about their newborn babies.Conclusion It is necessary to popularize the knowledge about labor analgesia,which is an important part of work on labor health.

45.0?5.4)was higher than those who underwent omnidistance tourniquet technology.The incidence rate of hematoma and venous thrombus of lower extremity had no obvious difference.[Conclusion]The postoperative blood loss can be reduced and early postoperative clinical effect and affected limb function can be improved by halfway tourniquet technology for patients who underwent TKA.

[Objective]To explore the "acute angle" of femur,and evaluate the bone tunnel technique in posterior cruciate ligament reconstruction.[Method]Using the porcine flexor digitorum tendon as graft,the author reconstructed posterior cruciate ligament.The bone tunnel was established with the angle of 80?,90?and 100?comparison the tunnel and intercondylar fossa of femur side wall,and press sensitive film was set between the edge of tunnel and graft.The pressure between was used to represent abrasion.The bigger the pressure was,the larger the abrasion was.The difference of pressure between bone tunnel technique group and the control group was compared.[Result]The pressure of angle 100?was lowest(3.55?0.21 MPa).And the pressure of burnishing group(3.29?0.19 MPa)was lower than that of the control group(3.55?0.21 MPa).[Conclusion]The bone tunnel technique can reduce the femoral;"acute angle" of femur in posterior cruciate ligament reconstruction.

AIM To investigate the effects of apoptosis and the expression of telomerase hTERT mRNA activity of HL-60 cells treated with As 2O 3 in different concentrations. METHODS HL-60 cells was cultured with As 2O 3 in different concentrations for 48 hours, then, cell apoptosis was detected with flow cytometry and the expression of telomerase hTERT mRNA was determined with RT-PCR respectively. RESULTS The percentage of the apoptosis in HL-60 cells were 2 28%?0 40%?2 55%?0 22% and 47 57%?2 79% respectively, as the cells were treated with 0 1 ?mol?L -1,1 0 ?mol?L -1,5 0 ?mol?L -1 As 2O 3 for 48 hours. The difference was significant between 5 0 ?mol?L -1 and 0 1～1 0 ?mol?L -1 groups(P