1.Review on processing mechanism of Herba Epimedii and new idea
Yan CHEN ; Xiaobin JIA ; Anwei DING
China Journal of Traditional Chinese Medicine and Pharmacy 2006;0(09):-
To elucidate the processing mechanism of Herba Epimedii from the new view of the intestinal absorption and metabolism.From analyzing the literatures of processing mechanism and togethering with the research of our lab,a new idea of processing mechanism of Herba Epimedii was brought:the pharmacologically active and easier absorbed flavonoids might be present more in the herbs when changing the heating processing parameters and thereby increased or maintained the efficacy.This thesis first pointed the new idea and method that the intestinal absorption and metabolism of herbs should be considered when studying the mechanism of processing.
2.Influence of intraoperative posture intervention on drainage in patients after gynecological laparoscopic surgery
Yun CHEN ; Ming ZHANG ; Yan DING
Chinese Journal of Nursing 2010;45(4):314-316
Objective To investigate the influence of intraoperative posture intervention on drainage in patients after gynecological laparoscopic surgery. Methods A total of 1250 patients who were scheduled for gynecological laparoscopic surgery including laparoscopic-assisted salpingoplasty,oophorocystectomy,ectopic pregnancy,vaginal hysterectomy,myomectomy and appendectomy were randomly divided into two groups. The patients' posture was changed from Trendelenburg position to level lithotomic position during suction by the circulating nurses in the experimental group,while after surgery in the control group. The volume and time of drainage,and the length of stay after surgery were compared between the two groups. Results The volume and time of drainage,as well as the length of stay after surgery had significant differences between the two groups (P<0.05). Conclusions Intraoperative posture intervention is helpful for the treatment effect of drainage after gynecological laparoscopic surgery.
3.Management of primary vesicoureteral reflux in children.
Yan CHEN ; Jie DING ; Jian-ping HUANG
Chinese Journal of Pediatrics 2004;42(10):750-752
4.Efficacy of PFNA and Gamma nail in treatment of intertrochanteric fractures in elderly
Hong DING ; Yingying HE ; Yan CHEN
Journal of Regional Anatomy and Operative Surgery 2014;(3):272-274
Objective to observe the efficacy and application value of PFNA and Gamma in the treatment of intertrochanteric fractures in elderly. Methods 100 patients with intertrochanteric fractures were divided into PFNA group and Gamma group, who received PFNA treatment and Gamma treatment respectively. And the operation status of patients at different ages,postoperative recovery,and complications were observed. Results The operation time,intraoperatve blood soss of PFNA group were lower than those of Gamma group(P<0. 05). There was no significant difference in fracture healing time. The Harris score of the over 75s in PFNA group was higher than that in Gamma group(P<0. 05),and the complications were less than Gamma group(P<0. 05). Conclusion PFNA was suitable for the patients at differ-ent ages with the advantages of more rigid fixation,good anti-rotation,fewer trauma and fewer complications.
5.Standardization of processing method for Pollen Typhae Carbonisatus
Hui YAN ; Peidong CHEN ; Anwei DING
Chinese Traditional and Herbal Drugs 1994;0(12):-
Objective To establish optimum processing method for Pollen Typhae Carbonisatus. Method Processing method was studied by orthogonal test and the total flavones were determined by HPLC. Conditions of HPLC used to determine the total flavones were: Waters Nove-Park C18 (150 mm?3.9 mm, 4 ?m), mobile phase: MeOH-THF-0.05%TFT (16∶24∶60), flow rate: 0.8 mL/min, column temperature: 30 ℃, detection wavelength: 360 nm. Results The optimum processing method was skir-baked for 8 min at 210 ℃. Conclusion The optimized processing method is available for the processing of Pollen Typhae Carbonisatus.
6.Establishment of Female Adult Rat Models with Acute Stress Reproductive- endocrine Disorder
Xiaoyuan LIAN ; Yan DING ; Qi CHEN ; Juntian ZHANG
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(03):-
Objective To observe the effects of acute stress on reproductive- endocrine function in female adult rats. Methods Acute immobilization stress was used to challenge the female adult rats and then the serum levels of progesterone (P), estradiol (E2) and corticosterone were measured 2 h after the stress.Results Acute stress significantly reduced E2 levels in each period of sexual cycle and increased the levels of P and corticosterone in the stressed rats.In addition, the ratio of estradiol and progesterone was disordered in stressed rats.Conclusion Acute stress can cause the disorder of estradiol and progesterone.Since stress is a major factor contributing to reproductive- endocrine disorder, this model will be useful for the research and development of drugs for reproductive- endocrine disorder.
7.Change of serum metalloproteinases and metalloproteinases inhibitors in exfoliation syndrome in Uyghur population
Yan, TANG ; Ruxin, DING ; Tingyu, XIE ; Xueyi, CHEN
Chinese Journal of Experimental Ophthalmology 2014;32(7):631-634
Background Exfoliation syndrome is a systemic disease with abnormal accumulation of extracellular matrix.Oxidative stress and imbalance between matrix metalloproteinases(MMPs) and tissue inhibitor of matrix metalloproteinases(TIMPs)may play an important role in the pathogenesis of exfoliation syndrome.Researches showed that the incidence of exfoliation syndrome is higher in Uyghur nationality than that in Han nationality.However,whether the imbalance of serum MMPs and TIMPs is associated with pathogenesis of different ethnic groups is unclear.Objective The aim of the study was to discuss the change of serum MMP-9 and TIMP-2 in Uyghur patients.Methods This study protocol was approved by Ethic Committee of Xinjiang Medical University and followed Declaration of Helsinki.A prospective cohort study was performed.Forty Uyghur nationality (46 eyes) with exfoliation syndrome were collected from March 2012 to May 2013 in Affiliated First Hospital of Xinjiang Medical University and First People Hospital of Kashi.Forty cases(40 eyes)age-and gender-matched normal volunteers were included in the same duration.The peripheral blood was collected under the informed consent.Serum MMP-9 and TIMP-2 levels were detected by ELISA,and the results between the two groups were compared using independent samples t test.Results Slit-lamp examination found that part of pigmentation was depigmented and white dandrufflike substance attached in the pupil margin in all the patients,and stripping white dandruff-like substance was deposited in the front surface of the lens capsule which distributed in 3 zones.The pupils were disk-shaped pupil,and the surrounding area was the ring granular and the middle was transparent area without ablative material after dilation.Serum MMP-9 levels were (57.88±18.63)μg/L in the exfoliation syndrome group and (9.35±2.78)μg/L in the normal control group;serum TIMP-2 levels were (17.36±4.66) μg/L in the exfoliation syndrome group and (25.73±3.59) μg/L in the control group.The ratios of MMP-9/TIMP-2 were 3.57± 1.45 in the exfoliation syndrome group and 0.37±0.11 in the control group,with statistically significant differences in serum MMP-9 and TIMP-2 levels as well as ratio of MMP-9/TIMP-2 between the two groups(t=11.52,-6.36,9.87,all at P=0.00).Conclusions The upregulation of serum MMP-9 and downregulation of serum TIMP-2 are found in Uyghur patients with exfoliation syndrome.It is verified that the imbalance of serum MMP-9 and TIMP-2 contributes to the pathogenesis of exfoliation syndrome in Uyghur patients.
8.Clinical observation of iron sucrose injection in treating old patients with iron deficiency anemia
Sheng LI ; Bo SUN ; Guoqiang YAN ; Ding CHEN
Chinese Journal of Postgraduates of Medicine 2011;34(7):26-27
Objective To observe the effect and adverse reactions of iron sucrose injection in treating old patients with iron deficiency anemia(IDA). Methods Thirty-two old patients with IDA were divided into injection group(17 patients)and oral group(15 patients)by random digits table. The effects of improvement on IDA and their adverse reactions were observed. Results After treatment, hemoglobin(Hb)in the injection group and oral group was(100.18±8.71)g/L and(85.80 ± 6.92)g/L(P<0.01), and serum iron was(23.84±5.34)μmol/L and(14.63±3.29)μmol/L(P<0.01).There were no obvious adverse reactions in injection group, whereas there were 6 cases(40%)with gastric and colon adverse reaction in oral group. Conclusions The treatment of intravenous injection of iron sucrose can be effectively used to cure old patients with IDA. It has better effect and has lower rate of adverse reaction.
9.Effects of erigeron on hippocampus adenosine triphosphate of gerbils with cerebral ischemia reperfusion injury
Yan ZHANG ; Qun CHEN ; Haozhong DING ; Yinming ZENG
Chinese Journal of Tissue Engineering Research 2005;9(41):176-178
BACKGROUND: Erigeron, the inhibitor of protein kinase C, functions to decrease the death of neurocytes caused by cerebral ischemia reperfusion.OBJECTIVE: To study the effects of erigeron on hippocampus adenosine triphosphate (ATP) and adenosine triphosphatase (ATPase) of gerbils with cerebral ischemia reperfusion injury.DESIGN: A random controlled experiment.SETTING: Anesthesia Department of the Fourth Hospital of Suzhou University, Anesthesia Department of the Fourth People's Hospital of Wuxi City, and Anesthesia Department of the Affiliated Hospital of Xuzhou Medical College.MATERIALS: The experiment was done in the key laboratory of anesthesiology of Jiangsu Province from March 2002 to May 2003. The 90 Mongolian gerbils were divided into 9 groups: sham operated group, cerebral ischemia group, cerebral ischemia reperfusion 1 hour group, cerebral ischemia reperfusion 12 hours group, cerebral ischemia reperfusion 24 hours group, erigeron + cerebral ischemia group, erigeron + cerebral ischemia reperfusion 1 hour group, erigeron + cerebral ischemia reperfusion 12 hours group, and erigeron + cerebral ischemia reperfusion 24 hours group,with 10 gerbils in each. The erigeron injection with flavone 4.5 g/mL was made by the Biomedicine Factory of Yunnan Province and numbered 990103.METHODS: Apart from the sham operated group, all groups were established the ischemia reperfusion models. The erigeron groups were abdominally injected erigeron 10 mL/kg 30 minutes before the ischemia. Meanwhile, the other groups were injected the normal saline 10 mL/kg. The 6 gerbils of each group were taken blood for testing ATP content and ATPase activity and the rest 4 were only examined the pathological changes of neurocytes of hippocampus CA1 region. During the ischemia, the temperature of drum membrane (reflecting the brain temperature) was monitored and kept at (37±0.2)℃ in order to avoid the influence of temperature on the result. The comparison among groups was shown with the single factor analysis of variance.CA1 region.of ATP content and ATPase activity: Compared with the cerebral ischemia reperfusion 24 hours group, the ATP content of the erigeron 1, 12, 24 hours groups was obviously increased [(0.25 ±0.08), (0.81 ±0.12), (0.58 ±0.07),(0.43±0.09) mmol/kg, P < 0.01], the Na+-K+-ATPase activity was obviously P < 0.01], and the Ca2+-ATPase activity was also obviously promoted days after ischemia, the findings under the low power lens were: the pyramidal belt of the cerebral ischemia reperfusion groups became thinner and loose and even disappeared, but that of the erigeron groups became obviously wider and thicker; the findings under the high power lens were: most of the pyramidal cells of the cerebral ischemia reperfusion groups were in the state of coagulation necrosis, and those survived pyramidal cells with complete nuclear membrane and clear nucleole were very few, but the survived pyramidal cells of the erigeron groups were obviously more than those of the cerebral ischemia reperfusion groups.CONCLUSION: Erigeron increases obviously the ATP content after ischemia reperfusion, especially at the early stage of reperfusion, and at the same time, the ATPase activity is also in an improved tendency, relieving the ischemia reperfusion injury and protecting the neurocytes.
10.Application of postoperative nutritional index in prognosis evaluation of colorectal cancer patients
Ming ZHANG ; Xinqiang ZHU ; Chuang DING ; Lingyong KONG ; Yan CHEN
Practical Oncology Journal 2017;31(2):131-136
Objective The objective of this study was to evaluate the prognostic value of postoperative prognostic nutritional index(p-PNI)in the prognosis of colorectal cancer patients.Methods Retrospective analytical cases came from 89 patients with radical resection of colorectal cancer confirmed by the pathological section in Nanjing Drum Tower Hospital Suqian City People's Hospital from August 2009 to August 2012.The p-PNI was calculated based on the results of blood routine and biochemical tests after the first visit to the hospital.The Youden index was calculated using the receiver operating characteristic curve(ROC),and the PNI value combined with the best sensitivity and specificity was used as the threshold for the PNI packet.Kaplan-Meier method was used to study the effect of p-PNI on postoperative prognosis of patients with colorectal cancer.Cox regression model was selected to study the effect of p-PNI on the prognosis of colorectal cancer patients after surgery.Results The p-PNI of 89 cases of colorectal cancer patients was 25.18~60.74(43.81±6.47).The p-PNI in patients(less than 70 years old)was significantly increased when the number of lymph node metastases was less than 4,the preoperative CEA was less than 5ng/mL,and the intraoperative hemorrhage was less than 150 mL(P<0.05).When the p-PNI value was 43,the Youden exponent was the largest.Data analysis showed that p-PNI was correlated with age,lymph node metastasis,intraoperative blood loss and preoperative CEA(P<0.05).The survival rate of patients with high p-PNI was higher than that of low p-PNI group(X2=15.639,P<0.001).Single factor and multivariate analysis confirmed that p-PNI was an independent factor in the prognosis of patients with colorectal cancer(HR=2.179,95% CI:1.057~3.561,P<0.001).Conclusion p-PNI is an independent prognostic factor in patients with colorectal cancer,which is associated with long-term prognosis after surgery.